Restriction map for
pF1KB8829
(Circular) (Six-base) MAPSORT of: pF1KB8829.seq Check: 6959 from: 1 to: 4424
pF1KB8829 4424 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 1462 1462
Size: 4424
Acc65I G'GTAC_C
Cuts at: 1440 1440
Size: 4424
AclI AA'CG_TT
Cuts at: 4264 4264
Size: 4424
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 594 1098 2249 2681 3428 3696 594
Size: 504 1151 432 747 268 1322
Fragments arranged by size:
1322 1151 747 504 432 268
AflIII A'CryG_T
Cuts at: 229 1776 2880 3857 229
Size: 1547 1104 977 796
Fragments arranged by size:
1547 1104 977 796
AgeI A'CCGG_T
Cuts at: 2812 2812
Size: 4424
AleI CACnn'nnGTG
Cuts at: 303 1157 303
Size: 854 3570
AlwNI CAG_nnn'CTG
Cuts at: 1382 1729 3296 1382
Size: 347 1567 2510
Fragments arranged by size:
2510 1567 347
ApaLI G'TGCA_C
Cuts at: 3194 3194
Size: 4424
ApoI r'AATT_y
Cuts at: 313 1316 1428 3777 4033 313
Size: 1003 112 2349 256 704
Fragments arranged by size:
2349 1003 704 256 112
AseI AT'TA_AT
Cuts at: 20 1412 20
Size: 1392 3032
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4424
AvaI C'yCGr_G
Cuts at: 1444 1444
Size: 4424
BamHI G'GATC_C
Cuts at: 1449 1449
Size: 4424
BanI G'GyrC_C
Cuts at: 1440 1854 2102 2137 1440
Size: 414 248 35 3727
Fragments arranged by size:
3727 414 248 35
BanII G_rGCy'C
Cuts at: 1438 2468 1438
Size: 1030 3394
BbeI G_GCGC'C
Cuts at: 1858 2106 1858
Size: 248 4176
BbsI GAAGACnn'nnnn_
Cuts at: 182 891 182
Size: 709 3715
BciVI GTATCCnnnnn_n'
Cuts at: 2322 3083 4424 2322
Size: 761 1341 2322
Fragments arranged by size:
2322 1341 761
BclI T'GATC_A
Cuts at: 1944 1944
Size: 4424
BglII A'GATC_T
Cuts at: 1117 1939 3976 1117
Size: 822 2037 1565
Fragments arranged by size:
2037 1565 822
BlpI GC'TnA_GC
Cuts at: 1536 1536
Size: 4424
Bme1580I G_kGCm'C
Cuts at: 2049 2142 3198 2049
Size: 93 1056 3275
Fragments arranged by size:
3275 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 662 782 950 1636 1717 2052 4196 662
Size: 120 168 686 81 335 2144 890
Fragments arranged by size:
2144 890 686 335 168 120 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 136 2791 136
Size: 2655 1769
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 590 926 1010 1094 1262 1346 224
Size: 366 336 84 84 168 84 3302
Fragments arranged by size:
3302 366 336 168 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 378 1600 2479 2971 3269 378
Size: 1222 879 492 298 1533
Fragments arranged by size:
1533 1222 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4128 4128
Size: 4424
BsaAI yAC'GTr
Cuts at: 1777 1777
Size: 4424
BsaBI GATnn'nnATC
Cuts at: 6 255 1938 3975 6
Size: 249 1683 2037 455
Fragments arranged by size:
2037 1683 455 249
BsaHI Gr'CG_yC
Cuts at: 1855 2103 2805 4301 1855
Size: 248 702 1496 1978
Fragments arranged by size:
1978 1496 702 248
BsaWI w'CCGG_w
Cuts at: 171 1613 2134 2812 3086 3233 3644 171
Size: 1442 521 678 274 147 411 951
Fragments arranged by size:
1442 951 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1849 2607 3184 3680 1849
Size: 758 577 496 2593
Fragments arranged by size:
2593 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 152
Size: 4424
BsiEI CG_ry'CG
Cuts at: 104 1966 2012 3220 104
Size: 1862 46 1208 1308
Fragments arranged by size:
1862 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1438 2216 2406 3198 1438
Size: 778 190 792 2664
Fragments arranged by size:
2664 792 778 190
BsmI GAATG_Cn'
Cuts at: 458 902 458
Size: 444 3980
Bsp1286I G_dGCh'C
Cuts at: 1438 2049 2142 2216 2406 2468 3198 1438
Size: 611 93 74 190 62 730 2664
Fragments arranged by size:
2664 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 171 3644 171
Size: 3473 951
BspHI T'CATG_A
Cuts at: 1056 3600 1056
Size: 2544 1880
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1474 1688 1990 2371 1474
Size: 214 302 381 3527
Fragments arranged by size:
3527 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2716 2770 4254 4419 50
Size: 2666 54 1484 165 55
Fragments arranged by size:
2666 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1021 1105 1189 2336 1021
Size: 84 84 1147 3109
Fragments arranged by size:
3109 1147 84 84
BsrFI r'CCGG_y
Cuts at: 2422 2603 2812 2422
Size: 181 209 4034
Fragments arranged by size:
4034 209 181
BssSI C'ACGA_G
Cuts at: 2695 3053 2695
Size: 358 4066
BstAPI GCAn_nnn'nTGC
Cuts at: 1801 1801
Size: 4424
BstBI TT'CG_AA
Cuts at: 378 462 630 882 1134 2785 378
Size: 84 168 252 252 1651 2017
Fragments arranged by size:
2017 1651 252 252 168 84
BstYI r'GATC_y
Cuts at: 1 1117 1449 1916 1939 2274 2520 3532 3618
Size: 1116 332 467 23 335 246 1012 86
Cuts at: 3618 3630 3976 1
Size: 12 346 449
Fragments arranged by size:
1116 1012 467 449 346 335 332 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4424
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2374 2555 2718 2374
Size: 181 163 4080
Fragments arranged by size:
4080 181 163
BtsI GCAGTG_nn'
Cuts at: 369 1667 369
Size: 1298 3126
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 999 1034 999
Size: 35 4389
DraI TTT'AAA
Cuts at: 1423 1423
Size: 4424
DrdI GACnn_nn'nnGTC
Cuts at: 2130 2988 2130
Size: 858 3566
EaeI y'GGCC_r
Cuts at: 2009 2183 2574 2601 3946 2009
Size: 174 391 27 1345 2487
Fragments arranged by size:
2487 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2009 2009
Size: 4424
EarI CTCTTCn'nnn_
Cuts at: 203 2447 2657 203
Size: 2244 210 1970
Fragments arranged by size:
2244 1970 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2632 2942 3088 4234 2632
Size: 310 146 1146 2822
Fragments arranged by size:
2822 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1436 1436
Size: 4424
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 590 594 926 1010 1094 1098 1262 1346
Size: 366 4 332 84 84 4 164 84
Cuts at: 1346 2249 2681 3428 3696 224
Size: 903 432 747 268 952
Fragments arranged by size:
952 903 747 432 366 332 268 164
84 84 84 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 264 264
Size: 4424
EcoO109I rG'GnC_Cy
Cuts at: 1563 1563
Size: 4424
EcoRI G'AATT_C
Cuts at: 1316 1428 1316
Size: 112 4312
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2443 2443
Size: 4424
FspI TGC'GCA
Cuts at: 2205 2205
Size: 4424
HaeII r_GCGC'y
Cuts at: 1858 2106 3128 4107 1858
Size: 248 1022 979 2175
Fragments arranged by size:
2175 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2266 2296 2298 2328 2266
Size: 30 2 30 4362
Fragments arranged by size:
4362 30 30 2
HincII GTy'rAC
Cuts at: 1463 1463
Size: 4424
HindIII A'AGCT_T
Cuts at: 876 876
Size: 4424
KasI G'GCGC_C
Cuts at: 1854 2102 1854
Size: 248 4176
KpnI G_GTAC'C
Cuts at: 1444 1444
Size: 4424
MfeI C'AATT_G
Cuts at: 780 780
Size: 4424
MluI A'CGCG_T
Cuts at: 3857 3857
Size: 4424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3095 3279 3095
Size: 184 4240
MscI TGG'CCA
Cuts at: 2185 2185
Size: 4424
MslI CAynn'nnrTG
Cuts at: 303 669 803 895 1157 1223 2413 2540 303
Size: 366 134 92 262 66 1190 127 2187
Fragments arranged by size:
2187 1190 366 262 134 127 92 66
MspA1I CmG'CkG
Cuts at: 1535 1849 2209 3222 3467 1535
Size: 314 360 1013 245 2492
Fragments arranged by size:
2492 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2605 2605
Size: 4424
NarI GG'CG_CC
Cuts at: 1855 2103 1855
Size: 248 4176
NcoI C'CATG_G
Cuts at: 107 107
Size: 4424
NdeI CA'TA_TG
Cuts at: 666 666
Size: 4424
NgoMIV G'CCGG_C
Cuts at: 2603 2603
Size: 4424
NspI r_CATG'y
Cuts at: 183 233 1477 1977 2884 183
Size: 50 1244 500 907 1723
Fragments arranged by size:
1723 1244 907 500 50
PciI A'CATG_T
Cuts at: 229 2880 229
Size: 2651 1773
PfoI T'CCnGG_A
Cuts at: 201 201
Size: 4424
PmeI GTTT'AAAC
Cuts at: 1423 1423
Size: 4424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3563 3595 3563
Size: 32 4392
PsiI TTA'TAA
Cuts at: 500 874 500
Size: 374 4050
PstI C_TGCA'G
Cuts at: 1471 2156 1471
Size: 685 3739
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4424
PvuII CAG'CTG
Cuts at: 1849 2209 1849
Size: 360 4064
RsrII CG'GwC_CG
Cuts at: 2619 2619
Size: 4424
SacI G_AGCT'C
Cuts at: 1438 1438
Size: 4424
SalI G'TCGA_C
Cuts at: 1461 1461
Size: 4424
SapI GCTCTTCn'nnn_
Cuts at: 2447 2657 2447
Size: 210 4214
SbfI CC_TGCA'GG
Cuts at: 1471 1471
Size: 4424
ScaI AGT'ACT
Cuts at: 3941 3941
Size: 4424
SfcI C'TryA_G
Cuts at: 33 1467 2152 3145 3336 33
Size: 1434 685 993 191 1121
Fragments arranged by size:
1434 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1856 2104 1856
Size: 248 4176
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4424
SmaI CCC'GGG
Cuts at: 1446 1446
Size: 4424
SmlI C'TyrA_G
Cuts at: 357 1579 2494 2986 3248 357
Size: 1222 915 492 262 1533
Fragments arranged by size:
1533 1222 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4424
SphI G_CATG'C
Cuts at: 1477 1977 1477
Size: 500 3924
StuI AGG'CCT
Cuts at: 393 915 393
Size: 522 3902
StyI C'CwwG_G
Cuts at: 107 524 1558 1912 107
Size: 417 1034 354 2619
Fragments arranged by size:
2619 1034 417 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 178 1079 2007 2140 2599 2808 178
Size: 901 928 133 459 209 1794
Fragments arranged by size:
1794 928 901 459 209 133
TatI w'GTAC_w
Cuts at: 1127 3939 1127
Size: 2812 1612
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 153 185 3941 3973 153
Size: 32 3756 32 604
Fragments arranged by size:
3756 604 32 32
Tth111I GACn'n_nGTC
Cuts at: 2221 2221
Size: 4424
XbaI T'CTAG_A
Cuts at: 1455 1455
Size: 4424
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1919 1919
Size: 4424
XmaI C'CCGG_G
Cuts at: 1444 1444
Size: 4424
XmnI GAAnn'nnTTC
Cuts at: 691 775 1027 1111 1279 1316 1363 691
Size: 84 252 84 168 37 47 3752
Fragments arranged by size:
3752 252 168 84 84 47 37
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PfoI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoRV FseI
FspAI HpaI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI