Restriction map for
pF1KB8818
(Circular) (Six-base) MAPSORT of: pF1KB8818.seq Check: 5168 from: 1 to: 4256
pF1KB8818 4256 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1294 1294
Size: 4256
Acc65I G'GTAC_C
Cuts at: 1272 1272
Size: 4256
AclI AA'CG_TT
Cuts at: 4096 4096
Size: 4256
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 125 1160 2081 2513 3260 3528 125
Size: 1035 921 432 747 268 853
Fragments arranged by size:
1035 921 853 747 432 268
AflII C'TTAA_G
Cuts at: 688 688
Size: 4256
AflIII A'CryG_T
Cuts at: 518 1608 2712 3689 518
Size: 1090 1104 977 1085
Fragments arranged by size:
1104 1090 1085 977
AgeI A'CCGG_T
Cuts at: 2644 2644
Size: 4256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 553 553
Size: 4256
AlwNI CAG_nnn'CTG
Cuts at: 152 1561 3128 152
Size: 1409 1567 1280
Fragments arranged by size:
1567 1409 1280
ApaLI G'TGCA_C
Cuts at: 3026 3026
Size: 4256
ApoI r'AATT_y
Cuts at: 366 377 868 1180 1260 3609 3865 366
Size: 11 491 312 80 2349 256 757
Fragments arranged by size:
2349 757 491 312 256 80 11
AseI AT'TA_AT
Cuts at: 20 575 20
Size: 555 3701
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4256
AvaI C'yCGr_G
Cuts at: 1276 1276
Size: 4256
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1199 1232 1199
Size: 33 4223
BamHI G'GATC_C
Cuts at: 1281 1281
Size: 4256
BanI G'GyrC_C
Cuts at: 1272 1686 1934 1969 1272
Size: 414 248 35 3559
Fragments arranged by size:
3559 414 248 35
BanII G_rGCy'C
Cuts at: 1270 2300 1270
Size: 1030 3226
BbeI G_GCGC'C
Cuts at: 1690 1938 1690
Size: 248 4008
BciVI GTATCCnnnnn_n'
Cuts at: 1186 2154 2915 4256 1186
Size: 968 761 1341 1186
Fragments arranged by size:
1341 1186 968 761
BclI T'GATC_A
Cuts at: 1776 1776
Size: 4256
BglII A'GATC_T
Cuts at: 1771 3808 1771
Size: 2037 2219
BlpI GC'TnA_GC
Cuts at: 1368 1368
Size: 4256
Bme1580I G_kGCm'C
Cuts at: 1881 1974 3030 1881
Size: 93 1056 3107
Fragments arranged by size:
3107 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1468 1549 1884 4028 1468
Size: 81 335 2144 1696
Fragments arranged by size:
2144 1696 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2623 2623
Size: 4256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1432 2311 2803 3101 1432
Size: 879 492 298 2587
Fragments arranged by size:
2587 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3960 3960
Size: 4256
BsaAI yAC'GTr
Cuts at: 1609 1609
Size: 4256
BsaBI GATnn'nnATC
Cuts at: 6 1770 3807 6
Size: 1764 2037 455
Fragments arranged by size:
2037 1764 455
BsaHI Gr'CG_yC
Cuts at: 1687 1935 2637 4133 1687
Size: 248 702 1496 1810
Fragments arranged by size:
1810 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1445 1966 2644 2918 3065 3476 1445
Size: 521 678 274 147 411 2225
Fragments arranged by size:
2225 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 153 153
Size: 4256
BseYI C'CCAG_C
Cuts at: 1681 2439 3016 3512 1681
Size: 758 577 496 2425
Fragments arranged by size:
2425 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1798 1844 3052 104
Size: 1694 46 1208 1308
Fragments arranged by size:
1694 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1270 2048 2238 3030 1270
Size: 778 190 792 2496
Fragments arranged by size:
2496 792 778 190
BsmI GAATG_Cn'
Cuts at: 179 179
Size: 4256
Bsp1286I G_dGCh'C
Cuts at: 1270 1881 1974 2048 2238 2300 3030 1270
Size: 611 93 74 190 62 730 2496
Fragments arranged by size:
2496 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3476 3476
Size: 4256
BspHI T'CATG_A
Cuts at: 3432 3432
Size: 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1306 1520 1822 2203 1306
Size: 214 302 381 3359
Fragments arranged by size:
3359 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2548 2602 4086 4251 50
Size: 2498 54 1484 165 55
Fragments arranged by size:
2498 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 398 2168 398
Size: 1770 2486
BsrFI r'CCGG_y
Cuts at: 2254 2435 2644 2254
Size: 181 209 3866
Fragments arranged by size:
3866 209 181
BsrGI T'GTAC_A
Cuts at: 521 521
Size: 4256
BssSI C'ACGA_G
Cuts at: 2527 2885 2527
Size: 358 3898
BstAPI GCAn_nnn'nTGC
Cuts at: 1633 1633
Size: 4256
BstBI TT'CG_AA
Cuts at: 2617 2617
Size: 4256
BstYI r'GATC_y
Cuts at: 1 1074 1281 1748 1771 2106 2352 3364 3450
Size: 1073 207 467 23 335 246 1012 86
Cuts at: 3450 3462 3808 1
Size: 12 346 449
Fragments arranged by size:
1073 1012 467 449 346 335 246 207
86 23 12
BtgI C'CryG_G
Cuts at: 248 248
Size: 4256
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2206 2387 2550 2206
Size: 181 163 3912
Fragments arranged by size:
3912 181 163
BtsI GCAGTG_nn'
Cuts at: 1226 1499 1226
Size: 273 3983
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 445 480 445
Size: 35 4221
DraI TTT'AAA
Cuts at: 1185 1255 1185
Size: 70 4186
DraIII CAC_nnn'GTG
Cuts at: 475 716 475
Size: 241 4015
DrdI GACnn_nn'nnGTC
Cuts at: 1962 2820 1962
Size: 858 3398
EaeI y'GGCC_r
Cuts at: 1841 2015 2406 2433 3778 1841
Size: 174 391 27 1345 2319
Fragments arranged by size:
2319 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1841 1841
Size: 4256
EarI CTCTTCn'nnn_
Cuts at: 2279 2489 2279
Size: 210 4046
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2464 2774 2920 4066 2464
Size: 310 146 1146 2654
Fragments arranged by size:
2654 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1268 1268
Size: 4256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 125 1160 2081 2513 3260 3528 125
Size: 1035 921 432 747 268 853
Fragments arranged by size:
1035 921 853 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1395 1395
Size: 4256
EcoRI G'AATT_C
Cuts at: 1260 1260
Size: 4256
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2275 2275
Size: 4256
FspI TGC'GCA
Cuts at: 2037 2037
Size: 4256
HaeII r_GCGC'y
Cuts at: 1690 1938 2960 3939 1690
Size: 248 1022 979 2007
Fragments arranged by size:
2007 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 636 668 2098 2128 2130 2160 636
Size: 32 1430 30 2 30 2732
Fragments arranged by size:
2732 1430 32 30 30 2
HincII GTy'rAC
Cuts at: 278 1295 278
Size: 1017 3239
HpaI GTT'AAC
Cuts at: 278 278
Size: 4256
KasI G'GCGC_C
Cuts at: 1686 1934 1686
Size: 248 4008
KpnI G_GTAC'C
Cuts at: 1276 1276
Size: 4256
MluI A'CGCG_T
Cuts at: 3689 3689
Size: 4256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2927 3111 2927
Size: 184 4072
MscI TGG'CCA
Cuts at: 2017 2017
Size: 4256
MslI CAynn'nnrTG
Cuts at: 127 775 2245 2372 127
Size: 648 1470 127 2011
Fragments arranged by size:
2011 1470 648 127
MspA1I CmG'CkG
Cuts at: 152 1367 1681 2041 3054 3299 152
Size: 1215 314 360 1013 245 1109
Fragments arranged by size:
1215 1109 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2437 2437
Size: 4256
NarI GG'CG_CC
Cuts at: 1687 1935 1687
Size: 248 4008
NcoI C'CATG_G
Cuts at: 248 248
Size: 4256
NgoMIV G'CCGG_C
Cuts at: 2435 2435
Size: 4256
NspI r_CATG'y
Cuts at: 522 1309 1809 2716 522
Size: 787 500 907 2062
Fragments arranged by size:
2062 907 787 500
PciI A'CATG_T
Cuts at: 518 2712 518
Size: 2194 2062
PmeI GTTT'AAAC
Cuts at: 1255 1255
Size: 4256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3395 3427 3395
Size: 32 4224
PsiI TTA'TAA
Cuts at: 184 184
Size: 4256
PstI C_TGCA'G
Cuts at: 1303 1988 1303
Size: 685 3571
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4256
PvuII CAG'CTG
Cuts at: 152 1681 2041 152
Size: 1529 360 2367
Fragments arranged by size:
2367 1529 360
RsrII CG'GwC_CG
Cuts at: 2451 2451
Size: 4256
SacI G_AGCT'C
Cuts at: 1270 1270
Size: 4256
SalI G'TCGA_C
Cuts at: 1293 1293
Size: 4256
SapI GCTCTTCn'nnn_
Cuts at: 2279 2489 2279
Size: 210 4046
SbfI CC_TGCA'GG
Cuts at: 1303 1303
Size: 4256
ScaI AGT'ACT
Cuts at: 1248 3773 1248
Size: 2525 1731
SfcI C'TryA_G
Cuts at: 33 896 1299 1984 2977 3168 33
Size: 863 403 685 993 191 1121
Fragments arranged by size:
1121 993 863 685 403 191
SfoI GGC'GCC
Cuts at: 1688 1936 1688
Size: 248 4008
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4256
SmaI CCC'GGG
Cuts at: 1278 1278
Size: 4256
SmlI C'TyrA_G
Cuts at: 688 1411 2326 2818 3080 688
Size: 723 915 492 262 1864
Fragments arranged by size:
1864 915 723 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4256
SphI G_CATG'C
Cuts at: 1309 1809 1309
Size: 500 3756
StyI C'CwwG_G
Cuts at: 248 1390 1744 248
Size: 1142 354 2760
Fragments arranged by size:
2760 1142 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 569 1839 1972 2431 2640 569
Size: 1270 133 459 209 2185
Fragments arranged by size:
2185 1270 459 209 133
TatI w'GTAC_w
Cuts at: 521 918 1246 3771 521
Size: 397 328 2525 1006
Fragments arranged by size:
2525 1006 397 328
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 459 491 3773 3805 459
Size: 32 3282 32 910
Fragments arranged by size:
3282 910 32 32
Tth111I GACn'n_nGTC
Cuts at: 2053 2053
Size: 4256
XbaI T'CTAG_A
Cuts at: 1287 1287
Size: 4256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1751 1751
Size: 4256
XmaI C'CCGG_G
Cuts at: 1276 1276
Size: 4256
XmnI GAAnn'nnTTC
Cuts at: 1019 1019
Size: 4256
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI EcoNI EcoRV FseI FspAI
HindIII MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI