Restriction map for
pF1KB8810
(Circular) (Six-base) MAPSORT of: pF1KB8810.seq Check: 9406 from: 1 to: 4196
pF1KB8810 4196 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 448 448
Size: 4196
AccI GT'mk_AC
Cuts at: 1234 1234
Size: 4196
Acc65I G'GTAC_C
Cuts at: 1212 1212
Size: 4196
AclI AA'CG_TT
Cuts at: 4036 4036
Size: 4196
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 762 1145 2021 2453 3200 3468 762
Size: 383 876 432 747 268 1490
Fragments arranged by size:
1490 876 747 432 383 268
AflIII A'CryG_T
Cuts at: 1548 2652 3629 1548
Size: 1104 977 2115
Fragments arranged by size:
2115 1104 977
AgeI A'CCGG_T
Cuts at: 2584 2584
Size: 4196
AlwNI CAG_nnn'CTG
Cuts at: 1123 1501 3068 1123
Size: 378 1567 2251
Fragments arranged by size:
2251 1567 378
ApaLI G'TGCA_C
Cuts at: 508 2966 508
Size: 2458 1738
ApoI r'AATT_y
Cuts at: 321 1131 1200 3549 3805 321
Size: 810 69 2349 256 712
Fragments arranged by size:
2349 810 712 256 69
AseI AT'TA_AT
Cuts at: 20 20
Size: 4196
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4196
AvaI C'yCGr_G
Cuts at: 1216 1216
Size: 4196
BamHI G'GATC_C
Cuts at: 1221 1221
Size: 4196
BanI G'GyrC_C
Cuts at: 1212 1626 1874 1909 1212
Size: 414 248 35 3499
Fragments arranged by size:
3499 414 248 35
BanII G_rGCy'C
Cuts at: 245 1210 2240 245
Size: 965 1030 2201
Fragments arranged by size:
2201 1030 965
BbeI G_GCGC'C
Cuts at: 1630 1878 1630
Size: 248 3948
BbsI GAAGACnn'nnnn_
Cuts at: 222 1169 222
Size: 947 3249
BciVI GTATCCnnnnn_n'
Cuts at: 668 2094 2855 4196 668
Size: 1426 761 1341 668
Fragments arranged by size:
1426 1341 761 668
BclI T'GATC_A
Cuts at: 1716 1716
Size: 4196
BfrBI ATG'CAT
Cuts at: 650 650
Size: 4196
BglII A'GATC_T
Cuts at: 761 1711 3748 761
Size: 950 2037 1209
Fragments arranged by size:
2037 1209 950
BlpI GC'TnA_GC
Cuts at: 1308 1308
Size: 4196
Bme1580I G_kGCm'C
Cuts at: 512 1821 1914 2970 512
Size: 1309 93 1056 1738
Fragments arranged by size:
1738 1309 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1408 1489 1824 3968 1408
Size: 81 335 2144 1636
Fragments arranged by size:
2144 1636 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2563 2563
Size: 4196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 417 417
Size: 4196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1372 2251 2743 3041 1372
Size: 879 492 298 2527
Fragments arranged by size:
2527 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3900 3900
Size: 4196
BsaAI yAC'GTr
Cuts at: 1138 1549 1138
Size: 411 3785
BsaBI GATnn'nnATC
Cuts at: 6 1710 3747 6
Size: 1704 2037 455
Fragments arranged by size:
2037 1704 455
BsaHI Gr'CG_yC
Cuts at: 1627 1875 2577 4073 1627
Size: 248 702 1496 1750
Fragments arranged by size:
1750 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1385 1906 2584 2858 3005 3416 1385
Size: 521 678 274 147 411 2165
Fragments arranged by size:
2165 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 587 617 587
Size: 30 4166
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 143 595 143
Size: 452 3744
BseYI C'CCAG_C
Cuts at: 1621 2379 2956 3452 1621
Size: 758 577 496 2365
Fragments arranged by size:
2365 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 961 961
Size: 4196
BsiEI CG_ry'CG
Cuts at: 104 1738 1784 2992 104
Size: 1634 46 1208 1308
Fragments arranged by size:
1634 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 512 1210 1988 2178 2970 512
Size: 698 778 190 792 1738
Fragments arranged by size:
1738 792 778 698 190
Bsp1286I G_dGCh'C
Cuts at: 245 512 1210 1821 1914 1988 2178 2240 2970
Size: 267 698 611 93 74 190 62 730
Cuts at: 2970 245
Size: 1471
Fragments arranged by size:
1471 730 698 611 267 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3416 3416
Size: 4196
BspHI T'CATG_A
Cuts at: 3372 3372
Size: 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 448 484 1246 1460 1762 2143 448
Size: 36 762 214 302 381 2501
Fragments arranged by size:
2501 762 381 302 214 36
BsrBI CCG'CTC
Cuts at: 50 2488 2542 4026 4191 50
Size: 2438 54 1484 165 55
Fragments arranged by size:
2438 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 389 497 2108 389
Size: 108 1611 2477
Fragments arranged by size:
2477 1611 108
BsrFI r'CCGG_y
Cuts at: 2194 2375 2584 2194
Size: 181 209 3806
Fragments arranged by size:
3806 209 181
BssSI C'ACGA_G
Cuts at: 244 2467 2825 244
Size: 2223 358 1615
Fragments arranged by size:
2223 1615 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1573 1573
Size: 4196
BstBI TT'CG_AA
Cuts at: 2557 2557
Size: 4196
BstYI r'GATC_y
Cuts at: 1 761 1221 1688 1711 2046 2292 3304 3390
Size: 760 460 467 23 335 246 1012 86
Cuts at: 3390 3402 3748 1
Size: 12 346 449
Fragments arranged by size:
1012 760 467 460 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 155 155
Size: 4196
BtgI C'CryG_G
Cuts at: 107 107
Size: 4196
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2146 2327 2490 2146
Size: 181 163 3852
Fragments arranged by size:
3852 181 163
BtsI GCAGTG_nn'
Cuts at: 1439 1439
Size: 4196
ClaI AT'CG_AT
Cuts at: 1096 1096
Size: 4196
DraI TTT'AAA
Cuts at: 1195 1195
Size: 4196
DrdI GACnn_nn'nnGTC
Cuts at: 1902 2760 1902
Size: 858 3338
EaeI y'GGCC_r
Cuts at: 194 1781 1955 2346 2373 3718 194
Size: 1587 174 391 27 1345 672
Fragments arranged by size:
1587 1345 672 391 174 27
EagI C'GGCC_G
Cuts at: 1781 1781
Size: 4196
EarI CTCTTCn'nnn_
Cuts at: 689 781 2219 2429 689
Size: 92 1438 210 2456
Fragments arranged by size:
2456 1438 210 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 128 2404 2714 2860 4006 128
Size: 2276 310 146 1146 318
Fragments arranged by size:
2276 1146 318 310 146
EcoICRI GAG'CTC
Cuts at: 1208 1208
Size: 4196
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 417 762 1145 2021 2453 3200 3468 417
Size: 345 383 876 432 747 268 1145
Fragments arranged by size:
1145 876 747 432 383 345 268
EcoNI CCTnn'n_nnAGG
Cuts at: 417 417
Size: 4196
EcoO109I rG'GnC_Cy
Cuts at: 1335 1335
Size: 4196
EcoRI G'AATT_C
Cuts at: 1200 1200
Size: 4196
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 4196
FspI TGC'GCA
Cuts at: 1977 1977
Size: 4196
HaeII r_GCGC'y
Cuts at: 1630 1878 2900 3879 1630
Size: 248 1022 979 1947
Fragments arranged by size:
1947 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2038 2068 2070 2100 2038
Size: 30 2 30 4134
Fragments arranged by size:
4134 30 30 2
HincII GTy'rAC
Cuts at: 1235 1235
Size: 4196
HindIII A'AGCT_T
Cuts at: 919 919
Size: 4196
KasI G'GCGC_C
Cuts at: 1626 1874 1626
Size: 248 3948
KpnI G_GTAC'C
Cuts at: 1216 1216
Size: 4196
MfeI C'AATT_G
Cuts at: 1005 1005
Size: 4196
MluI A'CGCG_T
Cuts at: 3629 3629
Size: 4196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2867 3051 2867
Size: 184 4012
MscI TGG'CCA
Cuts at: 1957 1957
Size: 4196
MslI CAynn'nnrTG
Cuts at: 502 2185 2312 502
Size: 1683 127 2386
Fragments arranged by size:
2386 1683 127
MspA1I CmG'CkG
Cuts at: 1307 1621 1981 2994 3239 1307
Size: 314 360 1013 245 2264
Fragments arranged by size:
2264 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2377 2377
Size: 4196
NarI GG'CG_CC
Cuts at: 1627 1875 1627
Size: 248 3948
NcoI C'CATG_G
Cuts at: 107 107
Size: 4196
NdeI CA'TA_TG
Cuts at: 453 908 453
Size: 455 3741
NgoMIV G'CCGG_C
Cuts at: 2375 2375
Size: 4196
NsiI A_TGCA'T
Cuts at: 652 652
Size: 4196
NspI r_CATG'y
Cuts at: 550 650 710 1043 1249 1749 2656 550
Size: 100 60 333 206 500 907 2090
Fragments arranged by size:
2090 907 500 333 206 100 60
PciI A'CATG_T
Cuts at: 2652 2652
Size: 4196
PfoI T'CCnGG_A
Cuts at: 938 938
Size: 4196
PmeI GTTT'AAAC
Cuts at: 1195 1195
Size: 4196
PmlI CAC'GTG
Cuts at: 1138 1138
Size: 4196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3335 3367 3335
Size: 32 4164
PsiI TTA'TAA
Cuts at: 736 736
Size: 4196
PstI C_TGCA'G
Cuts at: 1243 1928 1243
Size: 685 3511
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4196
PvuII CAG'CTG
Cuts at: 1621 1981 1621
Size: 360 3836
RsrII CG'GwC_CG
Cuts at: 2391 2391
Size: 4196
SacI G_AGCT'C
Cuts at: 1210 1210
Size: 4196
SalI G'TCGA_C
Cuts at: 1233 1233
Size: 4196
SapI GCTCTTCn'nnn_
Cuts at: 2219 2429 2219
Size: 210 3986
SbfI CC_TGCA'GG
Cuts at: 1243 1243
Size: 4196
ScaI AGT'ACT
Cuts at: 3713 3713
Size: 4196
SfcI C'TryA_G
Cuts at: 33 1239 1924 2917 3108 33
Size: 1206 685 993 191 1121
Fragments arranged by size:
1206 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1628 1876 1628
Size: 248 3948
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4196
SmaI CCC'GGG
Cuts at: 1218 1218
Size: 4196
SmlI C'TyrA_G
Cuts at: 1351 2266 2758 3020 1351
Size: 915 492 262 2527
Fragments arranged by size:
2527 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4196
SphI G_CATG'C
Cuts at: 1249 1749 1249
Size: 500 3696
StyI C'CwwG_G
Cuts at: 107 263 1330 1684 107
Size: 156 1067 354 2619
Fragments arranged by size:
2619 1067 354 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1779 1912 2371 2580 1779
Size: 133 459 209 3395
Fragments arranged by size:
3395 459 209 133
TatI w'GTAC_w
Cuts at: 723 3711 723
Size: 2988 1208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 587 619 3713 3745 587
Size: 32 3094 32 1038
Fragments arranged by size:
3094 1038 32 32
Tth111I GACn'n_nGTC
Cuts at: 1993 1993
Size: 4196
XbaI T'CTAG_A
Cuts at: 1227 1227
Size: 4196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1691 1691
Size: 4196
XmaI C'CCGG_G
Cuts at: 1216 1216
Size: 4196
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PfoI PmeI PmlI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I CspCI DraIII EcoRV FseI FspAI HpaI NheI
NotI NruI PacI PasI PflMI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI