Restriction map for
pF1KB8808
(Circular) (Six-base) MAPSORT of: pF1KB8808.seq Check: 3519 from: 1 to: 4178
pF1KB8808 4178 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 323 1160 1216 323
Size: 837 56 3285
Fragments arranged by size:
3285 837 56
Acc65I G'GTAC_C
Cuts at: 500 1194 500
Size: 694 3484
AclI AA'CG_TT
Cuts at: 4018 4018
Size: 4178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 656 802 2003 2435 3182 3450 656
Size: 146 1201 432 747 268 1384
Fragments arranged by size:
1384 1201 747 432 268 146
AflIII A'CryG_T
Cuts at: 1530 2634 3611 1530
Size: 1104 977 2097
Fragments arranged by size:
2097 1104 977
AgeI A'CCGG_T
Cuts at: 2566 2566
Size: 4178
AleI CACnn'nnGTG
Cuts at: 986 986
Size: 4178
AlwNI CAG_nnn'CTG
Cuts at: 1483 3050 1483
Size: 1567 2611
ApaLI G'TGCA_C
Cuts at: 2948 2948
Size: 4178
ApoI r'AATT_y
Cuts at: 1182 3531 3787 1182
Size: 2349 256 1573
Fragments arranged by size:
2349 1573 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4178
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
AvaI C'yCGr_G
Cuts at: 1198 1198
Size: 4178
AvrII C'CTAG_G
Cuts at: 1083 1099 1083
Size: 16 4162
BamHI G'GATC_C
Cuts at: 1203 1203
Size: 4178
BanI G'GyrC_C
Cuts at: 500 1194 1608 1856 1891 500
Size: 694 414 248 35 2787
Fragments arranged by size:
2787 694 414 248 35
BanII G_rGCy'C
Cuts at: 1192 2222 1192
Size: 1030 3148
BbeI G_GCGC'C
Cuts at: 1612 1860 1612
Size: 248 3930
BbsI GAAGACnn'nnnn_
Cuts at: 247 354 1095 247
Size: 107 741 3330
Fragments arranged by size:
3330 741 107
BbvCI CC'TCA_GC
Cuts at: 304 304
Size: 4178
BciVI GTATCCnnnnn_n'
Cuts at: 2076 2837 4178 2076
Size: 761 1341 2076
Fragments arranged by size:
2076 1341 761
BclI T'GATC_A
Cuts at: 1698 1698
Size: 4178
BglI GCCn_nnn'nGGC
Cuts at: 595 595
Size: 4178
BglII A'GATC_T
Cuts at: 692 1693 3730 692
Size: 1001 2037 1140
Fragments arranged by size:
2037 1140 1001
BlpI GC'TnA_GC
Cuts at: 1290 1290
Size: 4178
Bme1580I G_kGCm'C
Cuts at: 559 1803 1896 2952 559
Size: 1244 93 1056 1785
Fragments arranged by size:
1785 1244 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1390 1471 1806 3950 1390
Size: 81 335 2144 1618
Fragments arranged by size:
2144 1618 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2545 2545
Size: 4178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1137 1137
Size: 4178
Bpu10I CC'TnA_GC
Cuts at: 304 304
Size: 4178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 1354 2233 2725 3023 156
Size: 1198 879 492 298 1311
Fragments arranged by size:
1311 1198 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3882 3882
Size: 4178
BsaAI yAC'GTr
Cuts at: 1531 1531
Size: 4178
BsaBI GATnn'nnATC
Cuts at: 6 1692 3729 6
Size: 1686 2037 455
Fragments arranged by size:
2037 1686 455
BsaHI Gr'CG_yC
Cuts at: 1609 1857 2559 4055 1609
Size: 248 702 1496 1732
Fragments arranged by size:
1732 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1367 1888 2566 2840 2987 3398 1367
Size: 521 678 274 147 411 2147
Fragments arranged by size:
2147 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 321 1131 321
Size: 810 3368
BseYI C'CCAG_C
Cuts at: 1603 2361 2938 3434 1603
Size: 758 577 496 2347
Fragments arranged by size:
2347 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 757 757
Size: 4178
BsiEI CG_ry'CG
Cuts at: 104 1720 1766 2974 104
Size: 1616 46 1208 1308
Fragments arranged by size:
1616 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 830 1192 1970 2160 2952 830
Size: 362 778 190 792 2056
Fragments arranged by size:
2056 792 778 362 190
Bsp1286I G_dGCh'C
Cuts at: 559 830 1192 1803 1896 1970 2160 2222 2952
Size: 271 362 611 93 74 190 62 730
Cuts at: 2952 559
Size: 1785
Fragments arranged by size:
1785 730 611 362 271 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3398 3398
Size: 4178
BspHI T'CATG_A
Cuts at: 485 3354 485
Size: 2869 1309
BspMI ACCTGCnnnn'nnnn_
Cuts at: 359 1228 1442 1744 2125 359
Size: 869 214 302 381 2412
Fragments arranged by size:
2412 869 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2470 2524 4008 4173 50
Size: 2420 54 1484 165 55
Fragments arranged by size:
2420 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 607 839 2090 607
Size: 232 1251 2695
Fragments arranged by size:
2695 1251 232
BsrFI r'CCGG_y
Cuts at: 2176 2357 2566 2176
Size: 181 209 3788
Fragments arranged by size:
3788 209 181
BssSI C'ACGA_G
Cuts at: 2449 2807 2449
Size: 358 3820
BstAPI GCAn_nnn'nTGC
Cuts at: 903 1555 903
Size: 652 3526
BstBI TT'CG_AA
Cuts at: 2539 2539
Size: 4178
BstXI CCAn_nnnn'nTGG
Cuts at: 822 822
Size: 4178
BstYI r'GATC_y
Cuts at: 1 692 1203 1670 1693 2028 2274 3286 3372
Size: 691 511 467 23 335 246 1012 86
Cuts at: 3372 3384 3730 1
Size: 12 346 449
Fragments arranged by size:
1012 691 511 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 324 324
Size: 4178
BtgI C'CryG_G
Cuts at: 107 107
Size: 4178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2128 2309 2472 2128
Size: 181 163 3834
Fragments arranged by size:
3834 181 163
BtsI GCAGTG_nn'
Cuts at: 981 1421 981
Size: 440 3738
DraI TTT'AAA
Cuts at: 1177 1177
Size: 4178
DrdI GACnn_nn'nnGTC
Cuts at: 1884 2742 1884
Size: 858 3320
EaeI y'GGCC_r
Cuts at: 1763 1937 2328 2355 3700 1763
Size: 174 391 27 1345 2241
Fragments arranged by size:
2241 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1763 1763
Size: 4178
EarI CTCTTCn'nnn_
Cuts at: 778 2201 2411 778
Size: 1423 210 2545
Fragments arranged by size:
2545 1423 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2386 2696 2842 3988 2386
Size: 310 146 1146 2576
Fragments arranged by size:
2576 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1190 1190
Size: 4178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 656 802 1137 2003 2435 3182 3450 656
Size: 146 335 866 432 747 268 1384
Fragments arranged by size:
1384 866 747 432 335 268 146
EcoO109I rG'GnC_Cy
Cuts at: 861 1317 861
Size: 456 3722
EcoRI G'AATT_C
Cuts at: 1182 1182
Size: 4178
EcoRV GAT'ATC
Cuts at: 165 165
Size: 4178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 4178
FspI TGC'GCA
Cuts at: 1959 1959
Size: 4178
HaeII r_GCGC'y
Cuts at: 1612 1860 2882 3861 1612
Size: 248 1022 979 1929
Fragments arranged by size:
1929 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 154 186 372 404 2020 2050 2052 2082 154
Size: 32 186 32 1616 30 2 30 2250
Fragments arranged by size:
2250 1616 186 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1217 1217
Size: 4178
KasI G'GCGC_C
Cuts at: 1608 1856 1608
Size: 248 3930
KpnI G_GTAC'C
Cuts at: 504 1198 504
Size: 694 3484
MluI A'CGCG_T
Cuts at: 3611 3611
Size: 4178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2849 3033 2849
Size: 184 3994
MscI TGG'CCA
Cuts at: 1939 1939
Size: 4178
MslI CAynn'nnrTG
Cuts at: 484 490 748 986 2167 2294 484
Size: 6 258 238 1181 127 2368
Fragments arranged by size:
2368 1181 258 238 127 6
MspA1I CmG'CkG
Cuts at: 780 939 1289 1603 1963 2976 3221 780
Size: 159 350 314 360 1013 245 1737
Fragments arranged by size:
1737 1013 360 350 314 245 159
NaeI GCC'GGC
Cuts at: 2359 2359
Size: 4178
NarI GG'CG_CC
Cuts at: 1609 1857 1609
Size: 248 3930
NcoI C'CATG_G
Cuts at: 107 107
Size: 4178
NgoMIV G'CCGG_C
Cuts at: 2357 2357
Size: 4178
NspI r_CATG'y
Cuts at: 471 1231 1731 2638 471
Size: 760 500 907 2011
Fragments arranged by size:
2011 907 760 500
PciI A'CATG_T
Cuts at: 2634 2634
Size: 4178
PmeI GTTT'AAAC
Cuts at: 1177 1177
Size: 4178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 4146
PpuMI rG'GwC_Cy
Cuts at: 861 861
Size: 4178
PstI C_TGCA'G
Cuts at: 1225 1910 1225
Size: 685 3493
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4178
PvuII CAG'CTG
Cuts at: 780 939 1603 1963 780
Size: 159 664 360 2995
Fragments arranged by size:
2995 664 360 159
RsrII CG'GwC_CG
Cuts at: 2373 2373
Size: 4178
SacI G_AGCT'C
Cuts at: 1192 1192
Size: 4178
SalI G'TCGA_C
Cuts at: 1215 1215
Size: 4178
SanDI GG'GwC_CC
Cuts at: 861 861
Size: 4178
SapI GCTCTTCn'nnn_
Cuts at: 2201 2411 2201
Size: 210 3968
SbfI CC_TGCA'GG
Cuts at: 1225 1225
Size: 4178
ScaI AGT'ACT
Cuts at: 3695 3695
Size: 4178
SfcI C'TryA_G
Cuts at: 33 459 649 1221 1906 2899 3090 33
Size: 426 190 572 685 993 191 1121
Fragments arranged by size:
1121 993 685 572 426 191 190
SfoI GGC'GCC
Cuts at: 1610 1858 1610
Size: 248 3930
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
SmaI CCC'GGG
Cuts at: 1200 1200
Size: 4178
SmlI C'TyrA_G
Cuts at: 171 1333 2248 2740 3002 171
Size: 1162 915 492 262 1347
Fragments arranged by size:
1347 1162 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4178
SphI G_CATG'C
Cuts at: 1231 1731 1231
Size: 500 3678
SspI AAT'ATT
Cuts at: 1049 1049
Size: 4178
StyI C'CwwG_G
Cuts at: 107 809 1083 1099 1312 1666 107
Size: 702 274 16 213 354 2619
Fragments arranged by size:
2619 702 354 274 213 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 160 393 967 1761 1894 2353 2562 160
Size: 233 574 794 133 459 209 1776
Fragments arranged by size:
1776 794 574 459 233 209 133
TatI w'GTAC_w
Cuts at: 3693 3693
Size: 4178
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3695 3727 3695
Size: 32 4146
Tth111I GACn'n_nGTC
Cuts at: 1975 1975
Size: 4178
XbaI T'CTAG_A
Cuts at: 1209 1209
Size: 4178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 406 1673 406
Size: 1267 2911
XmaI C'CCGG_G
Cuts at: 1198 1198
Size: 4178
XmnI GAAnn'nnTTC
Cuts at: 194 694 1049 194
Size: 500 355 3323
Fragments arranged by size:
3323 500 355
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI BaeI BcgI BfrBI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII Bsu36I ClaI CspCI
DraIII EcoNI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI