Restriction map for
pF1KB8807
(Circular) (Six-base) MAPSORT of: pF1KB8807.seq Check: 1171 from: 1 to: 4178
pF1KB8807 4178 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1216 1216
Size: 4178
Acc65I G'GTAC_C
Cuts at: 142 500 1194 142
Size: 358 694 3126
Fragments arranged by size:
3126 694 358
AclI AA'CG_TT
Cuts at: 4018 4018
Size: 4178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 2003 2435 3182 3450 650
Size: 1353 432 747 268 1378
Fragments arranged by size:
1378 1353 747 432 268
AflIII A'CryG_T
Cuts at: 1530 2634 3611 1530
Size: 1104 977 2097
Fragments arranged by size:
2097 1104 977
AgeI A'CCGG_T
Cuts at: 2566 2566
Size: 4178
AlwNI CAG_nnn'CTG
Cuts at: 1483 3050 1483
Size: 1567 2611
ApaLI G'TGCA_C
Cuts at: 2948 2948
Size: 4178
ApoI r'AATT_y
Cuts at: 610 1182 3531 3787 610
Size: 572 2349 256 1001
Fragments arranged by size:
2349 1001 572 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4178
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
AvaI C'yCGr_G
Cuts at: 1198 1198
Size: 4178
BamHI G'GATC_C
Cuts at: 377 1203 377
Size: 826 3352
BanI G'GyrC_C
Cuts at: 142 500 791 1194 1608 1856 1891 142
Size: 358 291 403 414 248 35 2429
Fragments arranged by size:
2429 414 403 358 291 248 35
BanII G_rGCy'C
Cuts at: 540 1192 2222 540
Size: 652 1030 2496
Fragments arranged by size:
2496 1030 652
BbeI G_GCGC'C
Cuts at: 1612 1860 1612
Size: 248 3930
BbvCI CC'TCA_GC
Cuts at: 787 787
Size: 4178
BciVI GTATCCnnnnn_n'
Cuts at: 669 2076 2837 4178 669
Size: 1407 761 1341 669
Fragments arranged by size:
1407 1341 761 669
BclI T'GATC_A
Cuts at: 1698 1698
Size: 4178
BglI GCCn_nnn'nGGC
Cuts at: 257 257
Size: 4178
BglII A'GATC_T
Cuts at: 467 1693 3730 467
Size: 1226 2037 915
Fragments arranged by size:
2037 1226 915
BlpI GC'TnA_GC
Cuts at: 1290 1290
Size: 4178
Bme1580I G_kGCm'C
Cuts at: 221 796 1803 1896 2952 221
Size: 575 1007 93 1056 1447
Fragments arranged by size:
1447 1056 1007 575 93
BmgBI CAC'GTC
Cuts at: 165 165
Size: 4178
BmrI ACTGGGnnnn_n'
Cuts at: 204 411 791 1390 1471 1806 3950 204
Size: 207 380 599 81 335 2144 432
Fragments arranged by size:
2144 599 432 380 335 207 81
BmtI G_CTAG'C
Cuts at: 598 598
Size: 4178
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2545 2545
Size: 4178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 711 711
Size: 4178
Bpu10I CC'TnA_GC
Cuts at: 787 787
Size: 4178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1354 2233 2725 3023 1354
Size: 879 492 298 2509
Fragments arranged by size:
2509 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 127 3882 127
Size: 3755 423
BsaAI yAC'GTr
Cuts at: 1531 1531
Size: 4178
BsaBI GATnn'nnATC
Cuts at: 6 1692 3729 6
Size: 1686 2037 455
Fragments arranged by size:
2037 1686 455
BsaHI Gr'CG_yC
Cuts at: 1609 1857 2559 4055 1609
Size: 248 702 1496 1732
Fragments arranged by size:
1732 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1031 1367 1888 2566 2840 2987 3398 1031
Size: 336 521 678 274 147 411 1811
Fragments arranged by size:
1811 678 521 411 336 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 421 421
Size: 4178
BseYI C'CCAG_C
Cuts at: 1603 2361 2938 3434 1603
Size: 758 577 496 2347
Fragments arranged by size:
2347 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1720 1766 2974 104
Size: 1616 46 1208 1308
Fragments arranged by size:
1616 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 201 663 1192 1970 2160 2952 201
Size: 462 529 778 190 792 1427
Fragments arranged by size:
1427 792 778 529 462 190
Bsp1286I G_dGCh'C
Cuts at: 201 221 540 663 796 1192 1803 1896 1970
Size: 20 319 123 133 396 611 93 74
Cuts at: 1970 2160 2222 2952 201
Size: 190 62 730 1427
Fragments arranged by size:
1427 730 611 396 319 190 133 123
93 74 62 20
BspEI T'CCGG_A
Cuts at: 1031 3398 1031
Size: 2367 1811
BspHI T'CATG_A
Cuts at: 3354 3354
Size: 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 332 356 1049 1228 1442 1744 2125 332
Size: 24 693 179 214 302 381 2385
Fragments arranged by size:
2385 693 381 302 214 179 24
BsrBI CCG'CTC
Cuts at: 50 930 2470 2524 4008 4173 50
Size: 880 1540 54 1484 165 55
Fragments arranged by size:
1540 1484 880 165 55 54
BsrDI GCAATG_nn'
Cuts at: 267 2090 267
Size: 1823 2355
BsrFI r'CCGG_y
Cuts at: 2176 2357 2566 2176
Size: 181 209 3788
Fragments arranged by size:
3788 209 181
BsrGI T'GTAC_A
Cuts at: 458 458
Size: 4178
BssSI C'ACGA_G
Cuts at: 2449 2807 2449
Size: 358 3820
BstAPI GCAn_nnn'nTGC
Cuts at: 1555 1555
Size: 4178
BstBI TT'CG_AA
Cuts at: 2539 2539
Size: 4178
BstYI r'GATC_y
Cuts at: 1 377 467 1203 1670 1693 2028 2274 3286
Size: 376 90 736 467 23 335 246 1012
Cuts at: 3286 3372 3384 3730 1
Size: 86 12 346 449
Fragments arranged by size:
1012 736 467 449 376 346 335 246
90 86 23 12
BtgI C'CryG_G
Cuts at: 414 414
Size: 4178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2128 2309 2472 2128
Size: 181 163 3834
Fragments arranged by size:
3834 181 163
BtsI GCAGTG_nn'
Cuts at: 596 663 984 1421 596
Size: 67 321 437 3353
Fragments arranged by size:
3353 437 321 67
DraI TTT'AAA
Cuts at: 1177 1177
Size: 4178
DrdI GACnn_nn'nnGTC
Cuts at: 1884 2742 1884
Size: 858 3320
EaeI y'GGCC_r
Cuts at: 335 411 506 752 1763 1937 2328 2355 3700
Size: 76 95 246 1011 174 391 27 1345
Cuts at: 3700 335
Size: 813
Fragments arranged by size:
1345 1011 813 391 246 174 95 76
27
EagI C'GGCC_G
Cuts at: 1763 1763
Size: 4178
EarI CTCTTCn'nnn_
Cuts at: 358 608 626 638 897 2201 2411 358
Size: 250 18 12 259 1304 210 2125
Fragments arranged by size:
2125 1304 259 250 210 18 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2386 2696 2842 3988 2386
Size: 310 146 1146 2576
Fragments arranged by size:
2576 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1190 1190
Size: 4178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 711 2003 2435 3182 3450 650
Size: 61 1292 432 747 268 1378
Fragments arranged by size:
1378 1292 747 432 268 61
EcoO109I rG'GnC_Cy
Cuts at: 582 1317 582
Size: 735 3443
EcoRI G'AATT_C
Cuts at: 1182 1182
Size: 4178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 667 2197 667
Size: 1530 2648
FspI TGC'GCA
Cuts at: 1959 1959
Size: 4178
HaeII r_GCGC'y
Cuts at: 1612 1860 2882 3861 1612
Size: 248 1022 979 1929
Fragments arranged by size:
1929 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 924 956 2020 2050 2052 2082 924
Size: 32 1064 30 2 30 3020
Fragments arranged by size:
3020 1064 32 30 30 2
HincII GTy'rAC
Cuts at: 1217 1217
Size: 4178
KasI G'GCGC_C
Cuts at: 1608 1856 1608
Size: 248 3930
KpnI G_GTAC'C
Cuts at: 146 504 1198 146
Size: 358 694 3126
Fragments arranged by size:
3126 694 358
MluI A'CGCG_T
Cuts at: 3611 3611
Size: 4178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 999 2849 3033 999
Size: 1850 184 2144
Fragments arranged by size:
2144 1850 184
MscI TGG'CCA
Cuts at: 337 413 508 754 1939 337
Size: 76 95 246 1185 2576
Fragments arranged by size:
2576 1185 246 95 76
MslI CAynn'nnrTG
Cuts at: 844 958 2167 2294 844
Size: 114 1209 127 2728
Fragments arranged by size:
2728 1209 127 114
MspA1I CmG'CkG
Cuts at: 225 1289 1603 1963 2976 3221 225
Size: 1064 314 360 1013 245 1182
Fragments arranged by size:
1182 1064 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2359 2359
Size: 4178
NarI GG'CG_CC
Cuts at: 1609 1857 1609
Size: 248 3930
NcoI C'CATG_G
Cuts at: 414 414
Size: 4178
NgoMIV G'CCGG_C
Cuts at: 2357 2357
Size: 4178
NheI G'CTAG_C
Cuts at: 594 594
Size: 4178
NspI r_CATG'y
Cuts at: 423 1231 1731 2638 423
Size: 808 500 907 1963
Fragments arranged by size:
1963 907 808 500
PciI A'CATG_T
Cuts at: 2634 2634
Size: 4178
PmeI GTTT'AAAC
Cuts at: 1177 1177
Size: 4178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 4146
PstI C_TGCA'G
Cuts at: 1225 1910 1225
Size: 685 3493
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4178
PvuII CAG'CTG
Cuts at: 1603 1963 1603
Size: 360 3818
RsrII CG'GwC_CG
Cuts at: 2373 2373
Size: 4178
SacI G_AGCT'C
Cuts at: 1192 1192
Size: 4178
SalI G'TCGA_C
Cuts at: 1215 1215
Size: 4178
SapI GCTCTTCn'nnn_
Cuts at: 358 608 2201 2411 358
Size: 250 1593 210 2125
Fragments arranged by size:
2125 1593 250 210
SbfI CC_TGCA'GG
Cuts at: 1225 1225
Size: 4178
ScaI AGT'ACT
Cuts at: 3695 3695
Size: 4178
SexAI A'CCwGG_T
Cuts at: 947 947
Size: 4178
SfcI C'TryA_G
Cuts at: 33 1221 1906 2899 3090 33
Size: 1188 685 993 191 1121
Fragments arranged by size:
1188 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1610 1858 1610
Size: 248 3930
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
SmaI CCC'GGG
Cuts at: 1200 1200
Size: 4178
SmlI C'TyrA_G
Cuts at: 1333 2248 2740 3002 1333
Size: 915 492 262 2509
Fragments arranged by size:
2509 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4178
SphI G_CATG'C
Cuts at: 1231 1731 1231
Size: 500 3678
StyI C'CwwG_G
Cuts at: 414 1312 1666 414
Size: 898 354 2926
Fragments arranged by size:
2926 898 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1761 1894 2353 2562 1761
Size: 133 459 209 3377
Fragments arranged by size:
3377 459 209 133
TatI w'GTAC_w
Cuts at: 227 458 809 3693 227
Size: 231 351 2884 712
Fragments arranged by size:
2884 712 351 231
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3695 3727 3695
Size: 32 4146
Tth111I GACn'n_nGTC
Cuts at: 949 1975 949
Size: 1026 3152
XbaI T'CTAG_A
Cuts at: 1209 1209
Size: 4178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 201 1673 201
Size: 1472 2706
XmaI C'CCGG_G
Cuts at: 1198 1198
Size: 4178
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BsaXI
BsgI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI