Restriction map for
pF1KB8804
(Circular) (Six-base) MAPSORT of: pF1KB8804.seq Check: 8166 from: 1 to: 4163
pF1KB8804 4163 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 1201 1201
Size: 4163
Acc65I G'GTAC_C
Cuts at: 1179 1179
Size: 4163
AclI AA'CG_TT
Cuts at: 4003 4003
Size: 4163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 1988 2420 3167 3435 437
Size: 1551 432 747 268 1165
Fragments arranged by size:
1551 1165 747 432 268
AflIII A'CryG_T
Cuts at: 1515 2619 3596 1515
Size: 1104 977 2082
Fragments arranged by size:
2082 1104 977
AgeI A'CCGG_T
Cuts at: 2551 2551
Size: 4163
AlwNI CAG_nnn'CTG
Cuts at: 495 1468 3035 495
Size: 973 1567 1623
Fragments arranged by size:
1623 1567 973
ApaLI G'TGCA_C
Cuts at: 2933 2933
Size: 4163
ApoI r'AATT_y
Cuts at: 220 351 1167 3516 3772 220
Size: 131 816 2349 256 611
Fragments arranged by size:
2349 816 611 256 131
AseI AT'TA_AT
Cuts at: 20 20
Size: 4163
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
AvaI C'yCGr_G
Cuts at: 1183 1183
Size: 4163
AvrII C'CTAG_G
Cuts at: 681 681
Size: 4163
BamHI G'GATC_C
Cuts at: 1188 1188
Size: 4163
BanI G'GyrC_C
Cuts at: 1179 1593 1841 1876 1179
Size: 414 248 35 3466
Fragments arranged by size:
3466 414 248 35
BanII G_rGCy'C
Cuts at: 1177 2207 1177
Size: 1030 3133
BbeI G_GCGC'C
Cuts at: 1597 1845 1597
Size: 248 3915
BbsI GAAGACnn'nnnn_
Cuts at: 1089 1089
Size: 4163
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 936 970 936
Size: 34 4129
BciVI GTATCCnnnnn_n'
Cuts at: 2061 2822 4163 2061
Size: 761 1341 2061
Fragments arranged by size:
2061 1341 761
BclI T'GATC_A
Cuts at: 920 1683 920
Size: 763 3400
BglII A'GATC_T
Cuts at: 719 1678 3715 719
Size: 959 2037 1167
Fragments arranged by size:
2037 1167 959
BlpI GC'TnA_GC
Cuts at: 610 1275 610
Size: 665 3498
Bme1580I G_kGCm'C
Cuts at: 1788 1881 2937 1788
Size: 93 1056 3014
Fragments arranged by size:
3014 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1375 1456 1791 3935 1375
Size: 81 335 2144 1603
Fragments arranged by size:
2144 1603 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 4163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1098 1098
Size: 4163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1339 2218 2710 3008 1339
Size: 879 492 298 2494
Fragments arranged by size:
2494 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3867 3867
Size: 4163
BsaAI yAC'GTr
Cuts at: 1516 1516
Size: 4163
BsaBI GATnn'nnATC
Cuts at: 6 199 1677 3714 6
Size: 193 1478 2037 455
Fragments arranged by size:
2037 1478 455 193
BsaHI Gr'CG_yC
Cuts at: 1594 1842 2544 4040 1594
Size: 248 702 1496 1717
Fragments arranged by size:
1717 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1352 1873 2551 2825 2972 3383 1352
Size: 521 678 274 147 411 2132
Fragments arranged by size:
2132 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1117 1117
Size: 4163
BseYI C'CCAG_C
Cuts at: 541 620 1588 2346 2923 3419 541
Size: 79 968 758 577 496 1285
Fragments arranged by size:
1285 968 758 577 496 79
BsiEI CG_ry'CG
Cuts at: 104 1705 1751 2959 104
Size: 1601 46 1208 1308
Fragments arranged by size:
1601 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1177 1955 2145 2937 1177
Size: 778 190 792 2403
Fragments arranged by size:
2403 792 778 190
BsmI GAATG_Cn'
Cuts at: 491 659 491
Size: 168 3995
Bsp1286I G_dGCh'C
Cuts at: 1177 1788 1881 1955 2145 2207 2937 1177
Size: 611 93 74 190 62 730 2403
Fragments arranged by size:
2403 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3383 3383
Size: 4163
BspHI T'CATG_A
Cuts at: 788 3339 788
Size: 2551 1612
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1213 1427 1729 2110 1213
Size: 214 302 381 3266
Fragments arranged by size:
3266 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2455 2509 3993 4158 50
Size: 2405 54 1484 165 55
Fragments arranged by size:
2405 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2075 2075
Size: 4163
BsrFI r'CCGG_y
Cuts at: 2161 2342 2551 2161
Size: 181 209 3773
Fragments arranged by size:
3773 209 181
BsrGI T'GTAC_A
Cuts at: 341 461 341
Size: 120 4043
BssSI C'ACGA_G
Cuts at: 2434 2792 2434
Size: 358 3805
BstAPI GCAn_nnn'nTGC
Cuts at: 304 1540 304
Size: 1236 2927
BstBI TT'CG_AA
Cuts at: 2524 2524
Size: 4163
BstXI CCAn_nnnn'nTGG
Cuts at: 114 294 114
Size: 180 3983
BstYI r'GATC_y
Cuts at: 1 719 1188 1655 1678 2013 2259 3271 3357
Size: 718 469 467 23 335 246 1012 86
Cuts at: 3357 3369 3715 1
Size: 12 346 449
Fragments arranged by size:
1012 718 469 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 287 107
Size: 180 3983
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 939 963 2113 2294 2457 939
Size: 24 1150 181 163 2645
Fragments arranged by size:
2645 1150 181 163 24
BtsI GCAGTG_nn'
Cuts at: 1406 1406
Size: 4163
DraI TTT'AAA
Cuts at: 1162 1162
Size: 4163
DrdI GACnn_nn'nnGTC
Cuts at: 1869 2727 1869
Size: 858 3305
EaeI y'GGCC_r
Cuts at: 1058 1748 1922 2313 2340 3685 1058
Size: 690 174 391 27 1345 1536
Fragments arranged by size:
1536 1345 690 391 174 27
EagI C'GGCC_G
Cuts at: 1748 1748
Size: 4163
EarI CTCTTCn'nnn_
Cuts at: 2186 2396 2186
Size: 210 3953
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2371 2681 2827 3973 2371
Size: 310 146 1146 2561
Fragments arranged by size:
2561 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1175 1175
Size: 4163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 1098 1988 2420 3167 3435 437
Size: 661 890 432 747 268 1165
Fragments arranged by size:
1165 890 747 661 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1073 1126 1302 1073
Size: 53 176 3934
Fragments arranged by size:
3934 176 53
EcoRI G'AATT_C
Cuts at: 1167 1167
Size: 4163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 4163
FspI TGC'GCA
Cuts at: 1944 1944
Size: 4163
HaeII r_GCGC'y
Cuts at: 1597 1845 2867 3846 1597
Size: 248 1022 979 1914
Fragments arranged by size:
1914 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2005 2035 2037 2067 2005
Size: 30 2 30 4101
Fragments arranged by size:
4101 30 30 2
HincII GTy'rAC
Cuts at: 131 1202 131
Size: 1071 3092
KasI G'GCGC_C
Cuts at: 1593 1841 1593
Size: 248 3915
KpnI G_GTAC'C
Cuts at: 1183 1183
Size: 4163
MluI A'CGCG_T
Cuts at: 3596 3596
Size: 4163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2834 3018 2834
Size: 184 3979
MscI TGG'CCA
Cuts at: 1060 1924 1060
Size: 864 3299
MslI CAynn'nnrTG
Cuts at: 292 793 2152 2279 292
Size: 501 1359 127 2176
Fragments arranged by size:
2176 1359 501 127
MspA1I CmG'CkG
Cuts at: 624 929 995 1274 1588 1948 2961 3206 624
Size: 305 66 279 314 360 1013 245 1581
Fragments arranged by size:
1581 1013 360 314 305 279 245 66
NaeI GCC'GGC
Cuts at: 2344 2344
Size: 4163
NarI GG'CG_CC
Cuts at: 1594 1842 1594
Size: 248 3915
NcoI C'CATG_G
Cuts at: 107 287 107
Size: 180 3983
NgoMIV G'CCGG_C
Cuts at: 2342 2342
Size: 4163
NspI r_CATG'y
Cuts at: 1216 1716 2623 1216
Size: 500 907 2756
Fragments arranged by size:
2756 907 500
PciI A'CATG_T
Cuts at: 2619 2619
Size: 4163
PfoI T'CCnGG_A
Cuts at: 1075 1075
Size: 4163
PmeI GTTT'AAAC
Cuts at: 1162 1162
Size: 4163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 4131
PpuMI rG'GwC_Cy
Cuts at: 1073 1126 1073
Size: 53 4110
PshAI GACnn'nnGTC
Cuts at: 179 179
Size: 4163
PsiI TTA'TAA
Cuts at: 875 875
Size: 4163
PstI C_TGCA'G
Cuts at: 1210 1895 1210
Size: 685 3478
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4163
PvuII CAG'CTG
Cuts at: 624 929 995 1588 1948 624
Size: 305 66 593 360 2839
Fragments arranged by size:
2839 593 360 305 66
RsrII CG'GwC_CG
Cuts at: 2358 2358
Size: 4163
SacI G_AGCT'C
Cuts at: 1177 1177
Size: 4163
SalI G'TCGA_C
Cuts at: 1200 1200
Size: 4163
SapI GCTCTTCn'nnn_
Cuts at: 2186 2396 2186
Size: 210 3953
SbfI CC_TGCA'GG
Cuts at: 1210 1210
Size: 4163
ScaI AGT'ACT
Cuts at: 1146 3680 1146
Size: 2534 1629
SfcI C'TryA_G
Cuts at: 33 177 359 1130 1206 1891 2884 3075 33
Size: 144 182 771 76 685 993 191 1121
Fragments arranged by size:
1121 993 771 685 191 182 144 76
SfoI GGC'GCC
Cuts at: 1595 1843 1595
Size: 248 3915
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
SmaI CCC'GGG
Cuts at: 1185 1185
Size: 4163
SmlI C'TyrA_G
Cuts at: 1318 2233 2725 2987 1318
Size: 915 492 262 2494
Fragments arranged by size:
2494 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4163
SphI G_CATG'C
Cuts at: 1216 1716 1216
Size: 500 3663
StyI C'CwwG_G
Cuts at: 107 287 681 1297 1651 107
Size: 180 394 616 354 2619
Fragments arranged by size:
2619 616 394 354 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 407 1746 1879 2338 2547 407
Size: 1339 133 459 209 2023
Fragments arranged by size:
2023 1339 459 209 133
TatI w'GTAC_w
Cuts at: 341 461 1144 3678 341
Size: 120 683 2534 826
Fragments arranged by size:
2534 826 683 120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3680 3712 3680
Size: 32 4131
Tth111I GACn'n_nGTC
Cuts at: 1960 1960
Size: 4163
XbaI T'CTAG_A
Cuts at: 1194 1194
Size: 4163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1658 1658
Size: 4163
XmaI C'CCGG_G
Cuts at: 1183 1183
Size: 4163
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmBI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI