Restriction map for
pF1KB8803
(Circular) (Six-base) MAPSORT of: pF1KB8803.seq Check: 123 from: 1 to: 4160
pF1KB8803 4160 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1198 1198
Size: 4160
Acc65I G'GTAC_C
Cuts at: 1176 1176
Size: 4160
AclI AA'CG_TT
Cuts at: 4000 4000
Size: 4160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 806 1985 2417 3164 3432 616
Size: 190 1179 432 747 268 1344
Fragments arranged by size:
1344 1179 747 432 268 190
AflIII A'CryG_T
Cuts at: 1512 2616 3593 1512
Size: 1104 977 2079
Fragments arranged by size:
2079 1104 977
AgeI A'CCGG_T
Cuts at: 2548 2548
Size: 4160
AhdI GACnn_n'nnGTC
Cuts at: 658 658
Size: 4160
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 848 880 848
Size: 32 4128
AlwNI CAG_nnn'CTG
Cuts at: 1137 1465 3032 1137
Size: 328 1567 2265
Fragments arranged by size:
2265 1567 328
ApaLI G'TGCA_C
Cuts at: 2930 2930
Size: 4160
ApoI r'AATT_y
Cuts at: 281 1164 3513 3769 281
Size: 883 2349 256 672
Fragments arranged by size:
2349 883 672 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4160
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
AvaI C'yCGr_G
Cuts at: 224 1180 224
Size: 956 3204
BamHI G'GATC_C
Cuts at: 1185 1185
Size: 4160
BanI G'GyrC_C
Cuts at: 1176 1590 1838 1873 1176
Size: 414 248 35 3463
Fragments arranged by size:
3463 414 248 35
BanII G_rGCy'C
Cuts at: 584 816 1174 2204 584
Size: 232 358 1030 2540
Fragments arranged by size:
2540 1030 358 232
BbeI G_GCGC'C
Cuts at: 1594 1842 1594
Size: 248 3912
BciVI GTATCCnnnnn_n'
Cuts at: 2058 2819 4160 2058
Size: 761 1341 2058
Fragments arranged by size:
2058 1341 761
BclI T'GATC_A
Cuts at: 1680 1680
Size: 4160
BglI GCCn_nnn'nGGC
Cuts at: 880 880
Size: 4160
BglII A'GATC_T
Cuts at: 1675 3712 1675
Size: 2037 2123
BlpI GC'TnA_GC
Cuts at: 1272 1272
Size: 4160
Bme1580I G_kGCm'C
Cuts at: 647 760 1785 1878 2934 647
Size: 113 1025 93 1056 1873
Fragments arranged by size:
1873 1056 1025 113 93
BmrI ACTGGGnnnn_n'
Cuts at: 1093 1372 1453 1788 3932 1093
Size: 279 81 335 2144 1321
Fragments arranged by size:
2144 1321 335 279 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 4160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 836 730
Size: 106 4054
Bpu10I CC'TnA_GC
Cuts at: 1130 1130
Size: 4160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 281 671 1018 1336 2215 2707 3005 268
Size: 13 390 347 318 879 492 298 1423
Fragments arranged by size:
1423 879 492 390 347 318 298 13
BsaI GGTCTCn'nnnn_
Cuts at: 221 367 3864 221
Size: 146 3497 517
Fragments arranged by size:
3497 517 146
BsaAI yAC'GTr
Cuts at: 1513 1513
Size: 4160
BsaBI GATnn'nnATC
Cuts at: 6 433 1674 3711 6
Size: 427 1241 2037 455
Fragments arranged by size:
2037 1241 455 427
BsaHI Gr'CG_yC
Cuts at: 115 1591 1839 2541 4037 115
Size: 1476 248 702 1496 238
Fragments arranged by size:
1496 1476 702 248 238
BsaWI w'CCGG_w
Cuts at: 1349 1870 2548 2822 2969 3380 1349
Size: 521 678 274 147 411 2129
Fragments arranged by size:
2129 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 592 622 592
Size: 30 4130
BseYI C'CCAG_C
Cuts at: 775 1585 2343 2920 3416 775
Size: 810 758 577 496 1519
Fragments arranged by size:
1519 810 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1702 1748 2956 104
Size: 1598 46 1208 1308
Fragments arranged by size:
1598 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 584 1174 1952 2142 2934 584
Size: 590 778 190 792 1810
Fragments arranged by size:
1810 792 778 590 190
BsmBI CGTCTCn'nnnn_
Cuts at: 106 106
Size: 4160
Bsp1286I G_dGCh'C
Cuts at: 584 647 760 816 1174 1785 1878 1952 2142
Size: 63 113 56 358 611 93 74 190
Cuts at: 2142 2204 2934 584
Size: 62 730 1810
Fragments arranged by size:
1810 730 611 358 190 113 93 74
63 62 56
BspEI T'CCGG_A
Cuts at: 3380 3380
Size: 4160
BspHI T'CATG_A
Cuts at: 3336 3336
Size: 4160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1210 1424 1726 2107 1210
Size: 214 302 381 3263
Fragments arranged by size:
3263 381 302 214
BsrBI CCG'CTC
Cuts at: 50 948 2452 2506 3990 4155 50
Size: 898 1504 54 1484 165 55
Fragments arranged by size:
1504 1484 898 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2072 2072
Size: 4160
BsrFI r'CCGG_y
Cuts at: 2158 2339 2548 2158
Size: 181 209 3770
Fragments arranged by size:
3770 209 181
BssSI C'ACGA_G
Cuts at: 2431 2789 2431
Size: 358 3802
BstAPI GCAn_nnn'nTGC
Cuts at: 1537 1537
Size: 4160
BstBI TT'CG_AA
Cuts at: 2521 2521
Size: 4160
BstXI CCAn_nnnn'nTGG
Cuts at: 527 527
Size: 4160
BstYI r'GATC_y
Cuts at: 1 267 1053 1185 1652 1675 2010 2256 3268
Size: 266 786 132 467 23 335 246 1012
Cuts at: 3268 3354 3366 3712 1
Size: 86 12 346 449
Fragments arranged by size:
1012 786 467 449 346 335 266 246
132 86 23 12
BtgI C'CryG_G
Cuts at: 107 438 444 556 107
Size: 331 6 112 3711
Fragments arranged by size:
3711 331 112 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2110 2291 2454 2110
Size: 181 163 3816
Fragments arranged by size:
3816 181 163
BtsI GCAGTG_nn'
Cuts at: 1403 1403
Size: 4160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 148 183 148
Size: 35 4125
DraI TTT'AAA
Cuts at: 965 1159 965
Size: 194 3966
DrdI GACnn_nn'nnGTC
Cuts at: 1866 2724 1866
Size: 858 3302
EaeI y'GGCC_r
Cuts at: 441 734 957 1745 1919 2310 2337 3682 441
Size: 293 223 788 174 391 27 1345 919
Fragments arranged by size:
1345 919 788 391 293 223 174 27
EagI C'GGCC_G
Cuts at: 1745 1745
Size: 4160
EarI CTCTTCn'nnn_
Cuts at: 821 2183 2393 821
Size: 1362 210 2588
Fragments arranged by size:
2588 1362 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2368 2678 2824 3970 2368
Size: 310 146 1146 2558
Fragments arranged by size:
2558 1146 310 146
EcoICRI GAG'CTC
Cuts at: 582 1172 582
Size: 590 3570
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 730 806 836 1985 2417 3164 3432 616
Size: 114 76 30 1149 432 747 268 1344
Fragments arranged by size:
1344 1149 747 432 268 114 76 30
EcoNI CCTnn'n_nnAGG
Cuts at: 1036 1036
Size: 4160
EcoO109I rG'GnC_Cy
Cuts at: 1299 1299
Size: 4160
EcoRI G'AATT_C
Cuts at: 1164 1164
Size: 4160
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 646 2179 646
Size: 1533 2627
FspI TGC'GCA
Cuts at: 1141 1941 1141
Size: 800 3360
HaeII r_GCGC'y
Cuts at: 1594 1842 2864 3843 1594
Size: 248 1022 979 1911
Fragments arranged by size:
1911 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 644 676 2002 2032 2034 2064 644
Size: 32 1326 30 2 30 2740
Fragments arranged by size:
2740 1326 32 30 30 2
HincII GTy'rAC
Cuts at: 1199 1199
Size: 4160
HindIII A'AGCT_T
Cuts at: 383 383
Size: 4160
KasI G'GCGC_C
Cuts at: 1590 1838 1590
Size: 248 3912
KpnI G_GTAC'C
Cuts at: 1180 1180
Size: 4160
MfeI C'AATT_G
Cuts at: 131 131
Size: 4160
MluI A'CGCG_T
Cuts at: 3593 3593
Size: 4160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 923 2831 3015 923
Size: 1908 184 2068
Fragments arranged by size:
2068 1908 184
MscI TGG'CCA
Cuts at: 443 736 959 1921 443
Size: 293 223 962 2682
Fragments arranged by size:
2682 962 293 223
MslI CAynn'nnrTG
Cuts at: 2149 2276 2149
Size: 127 4033
MspA1I CmG'CkG
Cuts at: 636 1271 1585 1945 2958 3203 636
Size: 635 314 360 1013 245 1593
Fragments arranged by size:
1593 1013 635 360 314 245
NaeI GCC'GGC
Cuts at: 2341 2341
Size: 4160
NarI GG'CG_CC
Cuts at: 1591 1839 1591
Size: 248 3912
NcoI C'CATG_G
Cuts at: 107 444 556 107
Size: 337 112 3711
Fragments arranged by size:
3711 337 112
NgoMIV G'CCGG_C
Cuts at: 2339 2339
Size: 4160
NspI r_CATG'y
Cuts at: 1213 1713 2620 1213
Size: 500 907 2753
Fragments arranged by size:
2753 907 500
PciI A'CATG_T
Cuts at: 2616 2616
Size: 4160
PflMI CCAn_nnn'nTGG
Cuts at: 450 450
Size: 4160
PmeI GTTT'AAAC
Cuts at: 1159 1159
Size: 4160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3299 3331 3299
Size: 32 4128
PstI C_TGCA'G
Cuts at: 1207 1892 1207
Size: 685 3475
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4160
PvuII CAG'CTG
Cuts at: 636 1585 1945 636
Size: 949 360 2851
Fragments arranged by size:
2851 949 360
RsrII CG'GwC_CG
Cuts at: 2355 2355
Size: 4160
SacI G_AGCT'C
Cuts at: 584 1174 584
Size: 590 3570
SalI G'TCGA_C
Cuts at: 1197 1197
Size: 4160
SapI GCTCTTCn'nnn_
Cuts at: 821 2183 2393 821
Size: 1362 210 2588
Fragments arranged by size:
2588 1362 210
SbfI CC_TGCA'GG
Cuts at: 1207 1207
Size: 4160
ScaI AGT'ACT
Cuts at: 3677 3677
Size: 4160
SexAI A'CCwGG_T
Cuts at: 843 843
Size: 4160
SfcI C'TryA_G
Cuts at: 33 1203 1888 2881 3072 33
Size: 1170 685 993 191 1121
Fragments arranged by size:
1170 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1592 1840 1592
Size: 248 3912
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
SmaI CCC'GGG
Cuts at: 1182 1182
Size: 4160
SmlI C'TyrA_G
Cuts at: 247 296 686 1033 1315 2230 2722 2984 247
Size: 49 390 347 282 915 492 262 1423
Fragments arranged by size:
1423 915 492 390 347 282 262 49
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4160
SphI G_CATG'C
Cuts at: 1213 1713 1213
Size: 500 3660
StuI AGG'CCT
Cuts at: 970 970
Size: 4160
StyI C'CwwG_G
Cuts at: 107 444 556 1002 1294 1648 107
Size: 337 112 446 292 354 2619
Fragments arranged by size:
2619 446 354 337 292 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1743 1876 2335 2544 1743
Size: 133 459 209 3359
Fragments arranged by size:
3359 459 209 133
TatI w'GTAC_w
Cuts at: 3675 3675
Size: 4160
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 592 624 3677 3709 592
Size: 32 3053 32 1043
Fragments arranged by size:
3053 1043 32 32
Tth111I GACn'n_nGTC
Cuts at: 1957 1957
Size: 4160
XbaI T'CTAG_A
Cuts at: 285 1191 285
Size: 906 3254
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1000 1655 1000
Size: 655 3505
XmaI C'CCGG_G
Cuts at: 1180 1180
Size: 4160
XmnI GAAnn'nnTTC
Cuts at: 767 767
Size: 4160
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BseRI BsgI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI DraIII EcoRV FseI FspAI HpaI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI