Restriction map for
pF1KB8788
(Circular) (Six-base) MAPSORT of: pF1KB8788.seq Check: 7210 from: 1 to: 4013
pF1KB8788 4013 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 346 346
Size: 4013
AccI GT'mk_AC
Cuts at: 1051 1051
Size: 4013
Acc65I G'GTAC_C
Cuts at: 1029 1029
Size: 4013
AclI AA'CG_TT
Cuts at: 3853 3853
Size: 4013
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1838 2270 3017 3285 1838
Size: 432 747 268 2566
Fragments arranged by size:
2566 747 432 268
AflIII A'CryG_T
Cuts at: 1365 2469 3446 1365
Size: 1104 977 1932
Fragments arranged by size:
1932 1104 977
AgeI A'CCGG_T
Cuts at: 2401 2401
Size: 4013
AlwNI CAG_nnn'CTG
Cuts at: 356 1318 2885 356
Size: 962 1567 1484
Fragments arranged by size:
1567 1484 962
ApaI G_GGCC'C
Cuts at: 383 383
Size: 4013
ApaLI G'TGCA_C
Cuts at: 2783 2783
Size: 4013
ApoI r'AATT_y
Cuts at: 1017 3366 3622 1017
Size: 2349 256 1408
Fragments arranged by size:
2349 1408 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4013
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4013
AvaI C'yCGr_G
Cuts at: 260 1033 260
Size: 773 3240
BamHI G'GATC_C
Cuts at: 1038 1038
Size: 4013
BanI G'GyrC_C
Cuts at: 1029 1443 1691 1726 1029
Size: 414 248 35 3316
Fragments arranged by size:
3316 414 248 35
BanII G_rGCy'C
Cuts at: 308 383 1027 2057 308
Size: 75 644 1030 2264
Fragments arranged by size:
2264 1030 644 75
BbeI G_GCGC'C
Cuts at: 1447 1695 1447
Size: 248 3765
BciVI GTATCCnnnnn_n'
Cuts at: 1911 2672 4013 1911
Size: 761 1341 1911
Fragments arranged by size:
1911 1341 761
BclI T'GATC_A
Cuts at: 449 1533 449
Size: 1084 2929
BglII A'GATC_T
Cuts at: 1528 3565 1528
Size: 2037 1976
BlpI GC'TnA_GC
Cuts at: 1125 1125
Size: 4013
Bme1580I G_kGCm'C
Cuts at: 383 1638 1731 2787 383
Size: 1255 93 1056 1609
Fragments arranged by size:
1609 1255 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1225 1306 1641 3785 1225
Size: 81 335 2144 1453
Fragments arranged by size:
2144 1453 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 144 2380 144
Size: 2236 1777
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 284
Size: 4013
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1189 2068 2560 2858 1189
Size: 879 492 298 2344
Fragments arranged by size:
2344 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 272 3717 272
Size: 3445 568
BsaAI yAC'GTr
Cuts at: 1366 1366
Size: 4013
BsaBI GATnn'nnATC
Cuts at: 6 1527 3564 6
Size: 1521 2037 455
Fragments arranged by size:
2037 1521 455
BsaHI Gr'CG_yC
Cuts at: 1444 1692 2394 3890 1444
Size: 248 702 1496 1567
Fragments arranged by size:
1567 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1202 1723 2401 2675 2822 3233 1202
Size: 521 678 274 147 411 1982
Fragments arranged by size:
1982 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 357 439 442 357
Size: 82 3 3928
Fragments arranged by size:
3928 82 3
BseYI C'CCAG_C
Cuts at: 408 1438 2196 2773 3269 408
Size: 1030 758 577 496 1152
Fragments arranged by size:
1152 1030 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 706 673
Size: 33 3980
BsiEI CG_ry'CG
Cuts at: 104 1555 1601 2809 104
Size: 1451 46 1208 1308
Fragments arranged by size:
1451 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1027 1805 1995 2787 1027
Size: 778 190 792 2253
Fragments arranged by size:
2253 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 308 383 1027 1638 1731 1805 1995 2057 2787
Size: 75 644 611 93 74 190 62 730
Cuts at: 2787 308
Size: 1534
Fragments arranged by size:
1534 730 644 611 190 93 75 74
62
BspEI T'CCGG_A
Cuts at: 3233 3233
Size: 4013
BspHI T'CATG_A
Cuts at: 3189 3189
Size: 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 346 1063 1277 1579 1960 346
Size: 717 214 302 381 2399
Fragments arranged by size:
2399 717 381 302 214
BsrBI CCG'CTC
Cuts at: 50 128 2305 2359 3843 4008 50
Size: 78 2177 54 1484 165 55
Fragments arranged by size:
2177 1484 165 78 55 54
BsrDI GCAATG_nn'
Cuts at: 1925 1925
Size: 4013
BsrFI r'CCGG_y
Cuts at: 2011 2192 2401 2011
Size: 181 209 3623
Fragments arranged by size:
3623 209 181
BssSI C'ACGA_G
Cuts at: 2284 2642 2284
Size: 358 3655
BstAPI GCAn_nnn'nTGC
Cuts at: 1390 1390
Size: 4013
BstBI TT'CG_AA
Cuts at: 2374 2374
Size: 4013
BstYI r'GATC_y
Cuts at: 1 1038 1505 1528 1863 2109 3121 3207 3219
Size: 1037 467 23 335 246 1012 86 12
Cuts at: 3219 3565 1
Size: 346 449
Fragments arranged by size:
1037 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 133 133
Size: 4013
BtgI C'CryG_G
Cuts at: 107 107
Size: 4013
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1963 2144 2307 1963
Size: 181 163 3669
Fragments arranged by size:
3669 181 163
BtsI GCAGTG_nn'
Cuts at: 694 1256 694
Size: 562 3451
DraI TTT'AAA
Cuts at: 927 1012 927
Size: 85 3928
DrdI GACnn_nn'nnGTC
Cuts at: 1719 2577 1719
Size: 858 3155
EaeI y'GGCC_r
Cuts at: 1598 1772 2163 2190 3535 1598
Size: 174 391 27 1345 2076
Fragments arranged by size:
2076 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1598 1598
Size: 4013
EarI CTCTTCn'nnn_
Cuts at: 506 752 809 2036 2246 506
Size: 246 57 1227 210 2273
Fragments arranged by size:
2273 1227 246 210 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2221 2531 2677 3823 2221
Size: 310 146 1146 2411
Fragments arranged by size:
2411 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1025 1025
Size: 4013
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 1838 2270 3017 3285 284
Size: 1554 432 747 268 1012
Fragments arranged by size:
1554 1012 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 196 196
Size: 4013
EcoO109I rG'GnC_Cy
Cuts at: 379 380 1152 379
Size: 1 772 3240
Fragments arranged by size:
3240 772 1
EcoRI G'AATT_C
Cuts at: 1017 1017
Size: 4013
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 477 2032 477
Size: 1555 2458
FspI TGC'GCA
Cuts at: 1794 1794
Size: 4013
HaeII r_GCGC'y
Cuts at: 1447 1695 2717 3696 1447
Size: 248 1022 979 1764
Fragments arranged by size:
1764 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 188 220 1855 1885 1887 1917 188
Size: 32 1635 30 2 30 2284
Fragments arranged by size:
2284 1635 32 30 30 2
HincII GTy'rAC
Cuts at: 1052 1052
Size: 4013
KasI G'GCGC_C
Cuts at: 1443 1691 1443
Size: 248 3765
KpnI G_GTAC'C
Cuts at: 1033 1033
Size: 4013
MluI A'CGCG_T
Cuts at: 3446 3446
Size: 4013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 497 2684 2868 497
Size: 2187 184 1642
Fragments arranged by size:
2187 1642 184
MscI TGG'CCA
Cuts at: 1774 1774
Size: 4013
MslI CAynn'nnrTG
Cuts at: 2002 2129 2002
Size: 127 3886
MspA1I CmG'CkG
Cuts at: 356 596 1124 1438 1798 2811 3056 356
Size: 240 528 314 360 1013 245 1313
Fragments arranged by size:
1313 1013 528 360 314 245 240
NaeI GCC'GGC
Cuts at: 2194 2194
Size: 4013
NarI GG'CG_CC
Cuts at: 1444 1692 1444
Size: 248 3765
NcoI C'CATG_G
Cuts at: 107 107
Size: 4013
NgoMIV G'CCGG_C
Cuts at: 2192 2192
Size: 4013
NspI r_CATG'y
Cuts at: 1066 1566 2473 1066
Size: 500 907 2606
Fragments arranged by size:
2606 907 500
PasI CC'CwG_GG
Cuts at: 376 376
Size: 4013
PciI A'CATG_T
Cuts at: 2469 2469
Size: 4013
PmeI GTTT'AAAC
Cuts at: 1012 1012
Size: 4013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3152 3184 3152
Size: 32 3981
PspOMI G'GGCC_C
Cuts at: 379 379
Size: 4013
PstI C_TGCA'G
Cuts at: 1060 1745 1060
Size: 685 3328
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4013
PvuII CAG'CTG
Cuts at: 1438 1798 1438
Size: 360 3653
RsrII CG'GwC_CG
Cuts at: 2208 2208
Size: 4013
SacI G_AGCT'C
Cuts at: 1027 1027
Size: 4013
SalI G'TCGA_C
Cuts at: 1050 1050
Size: 4013
SapI GCTCTTCn'nnn_
Cuts at: 2036 2246 2036
Size: 210 3803
SbfI CC_TGCA'GG
Cuts at: 1060 1060
Size: 4013
ScaI AGT'ACT
Cuts at: 3530 3530
Size: 4013
SfcI C'TryA_G
Cuts at: 33 1056 1741 2734 2925 33
Size: 1023 685 993 191 1121
Fragments arranged by size:
1121 1023 993 685 191
SfoI GGC'GCC
Cuts at: 1445 1693 1445
Size: 248 3765
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4013
SmaI CCC'GGG
Cuts at: 262 1035 262
Size: 773 3240
SmlI C'TyrA_G
Cuts at: 1168 2083 2575 2837 1168
Size: 915 492 262 2344
Fragments arranged by size:
2344 915 492 262
SpeI A'CTAG_T
Cuts at: 67 807 67
Size: 740 3273
SphI G_CATG'C
Cuts at: 1066 1566 1066
Size: 500 3513
StyI C'CwwG_G
Cuts at: 107 192 317 1147 1501 107
Size: 85 125 830 354 2619
Fragments arranged by size:
2619 830 354 125 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1596 1729 2188 2397 1596
Size: 133 459 209 3212
Fragments arranged by size:
3212 459 209 133
TatI w'GTAC_w
Cuts at: 3528 3528
Size: 4013
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3530 3562 3530
Size: 32 3981
Tth111I GACn'n_nGTC
Cuts at: 1810 1810
Size: 4013
XbaI T'CTAG_A
Cuts at: 214 1044 214
Size: 830 3183
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 324 483 1508 324
Size: 159 1025 2829
Fragments arranged by size:
2829 1025 159
XmaI C'CCGG_G
Cuts at: 260 1033 260
Size: 773 3240
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI