Restriction map for
pF1KB8764
(Circular) (Six-base) MAPSORT of: pF1KB8764.seq Check: 7605 from: 1 to: 3767
pF1KB8764 3767 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 346 348 531 346
Size: 2 183 3582
Fragments arranged by size:
3582 183 2
AccI GT'mk_AC
Cuts at: 805 805
Size: 3767
Acc65I G'GTAC_C
Cuts at: 783 783
Size: 3767
AclI AA'CG_TT
Cuts at: 3607 3607
Size: 3767
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1592 2024 2771 3039 1592
Size: 432 747 268 2320
Fragments arranged by size:
2320 747 432 268
AflIII A'CryG_T
Cuts at: 1119 2223 3200 1119
Size: 1104 977 1686
Fragments arranged by size:
1686 1104 977
AgeI A'CCGG_T
Cuts at: 2155 2155
Size: 3767
AlwNI CAG_nnn'CTG
Cuts at: 1072 2639 1072
Size: 1567 2200
ApaI G_GGCC'C
Cuts at: 494 494
Size: 3767
ApaLI G'TGCA_C
Cuts at: 2537 2537
Size: 3767
ApoI r'AATT_y
Cuts at: 771 3120 3376 771
Size: 2349 256 1162
Fragments arranged by size:
2349 1162 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3767
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3767
AvaI C'yCGr_G
Cuts at: 787 787
Size: 3767
BamHI G'GATC_C
Cuts at: 474 792 474
Size: 318 3449
BanI G'GyrC_C
Cuts at: 259 314 388 547 783 1197 1445 1480 259
Size: 55 74 159 236 414 248 35 2546
Fragments arranged by size:
2546 414 248 236 159 74 55 35
BanII G_rGCy'C
Cuts at: 494 648 781 1811 494
Size: 154 133 1030 2450
Fragments arranged by size:
2450 1030 154 133
BbeI G_GCGC'C
Cuts at: 1201 1449 1201
Size: 248 3519
BbsI GAAGACnn'nnnn_
Cuts at: 128 128
Size: 3767
BciVI GTATCCnnnnn_n'
Cuts at: 1665 2426 3767 1665
Size: 761 1341 1665
Fragments arranged by size:
1665 1341 761
BclI T'GATC_A
Cuts at: 1287 1287
Size: 3767
BglI GCCn_nnn'nGGC
Cuts at: 350 489 350
Size: 139 3628
BglII A'GATC_T
Cuts at: 1282 3319 1282
Size: 2037 1730
BlpI GC'TnA_GC
Cuts at: 879 879
Size: 3767
Bme1580I G_kGCm'C
Cuts at: 262 317 391 494 550 1392 1485 2541 262
Size: 55 74 103 56 842 93 1056 1488
Fragments arranged by size:
1488 1056 842 103 93 74 56 55
BmrI ACTGGGnnnn_n'
Cuts at: 531 979 1060 1395 3539 531
Size: 448 81 335 2144 759
Fragments arranged by size:
2144 759 448 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2134 2134
Size: 3767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 556 670 694 457
Size: 99 114 24 3530
Fragments arranged by size:
3530 114 99 24
Bpu10I CC'TnA_GC
Cuts at: 589 589
Size: 3767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 1822 2314 2612 943
Size: 879 492 298 2098
Fragments arranged by size:
2098 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3471 3471
Size: 3767
BsaAI yAC'GTr
Cuts at: 1120 1120
Size: 3767
BsaBI GATnn'nnATC
Cuts at: 6 1281 3318 6
Size: 1275 2037 455
Fragments arranged by size:
2037 1275 455
BsaHI Gr'CG_yC
Cuts at: 1198 1446 2148 3644 1198
Size: 248 702 1496 1321
Fragments arranged by size:
1496 1321 702 248
BsaWI w'CCGG_w
Cuts at: 956 1477 2155 2429 2576 2987 956
Size: 521 678 274 147 411 1736
Fragments arranged by size:
1736 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 194 224 667 697 194
Size: 30 443 30 3264
Fragments arranged by size:
3264 443 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 148 193 445 667 148
Size: 45 252 222 3248
Fragments arranged by size:
3248 252 222 45
BseYI C'CCAG_C
Cuts at: 310 460 494 1192 1950 2527 3023 310
Size: 150 34 698 758 577 496 1054
Fragments arranged by size:
1054 758 698 577 496 150 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 380 380
Size: 3767
BsiEI CG_ry'CG
Cuts at: 104 1309 1355 2563 104
Size: 1205 46 1208 1308
Fragments arranged by size:
1308 1208 1205 46
BsiHKAI G_wGCw'C
Cuts at: 781 1559 1749 2541 781
Size: 778 190 792 2007
Fragments arranged by size:
2007 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 262 317 391 494 550 648 781 1392 1485
Size: 55 74 103 56 98 133 611 93
Cuts at: 1485 1559 1749 1811 2541 262
Size: 74 190 62 730 1488
Fragments arranged by size:
1488 730 611 190 133 103 98 93
74 74 62 56 55
BspEI T'CCGG_A
Cuts at: 2987 2987
Size: 3767
BspHI T'CATG_A
Cuts at: 2943 2943
Size: 3767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 346 348 531 817 1031 1333 1714 346
Size: 2 183 286 214 302 381 2399
Fragments arranged by size:
2399 381 302 286 214 183 2
BsrBI CCG'CTC
Cuts at: 50 2059 2113 3597 3762 50
Size: 2009 54 1484 165 55
Fragments arranged by size:
2009 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1679 1679
Size: 3767
BsrFI r'CCGG_y
Cuts at: 1765 1946 2155 1765
Size: 181 209 3377
Fragments arranged by size:
3377 209 181
BssSI C'ACGA_G
Cuts at: 305 2038 2396 305
Size: 1733 358 1676
Fragments arranged by size:
1733 1676 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1144 1144
Size: 3767
BstBI TT'CG_AA
Cuts at: 2128 2128
Size: 3767
BstYI r'GATC_y
Cuts at: 1 474 792 1259 1282 1617 1863 2875 2961
Size: 473 318 467 23 335 246 1012 86
Cuts at: 2961 2973 3319 1
Size: 12 346 449
Fragments arranged by size:
1012 473 467 449 346 335 318 246
86 23 12
BtgI C'CryG_G
Cuts at: 366 450 486 551 366
Size: 84 36 65 3582
Fragments arranged by size:
3582 84 65 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1717 1898 2061 1717
Size: 181 163 3423
Fragments arranged by size:
3423 181 163
BtsI GCAGTG_nn'
Cuts at: 392 611 1010 392
Size: 219 399 3149
Fragments arranged by size:
3149 399 219
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 492 527 492
Size: 35 3732
DraI TTT'AAA
Cuts at: 766 766
Size: 3767
DrdI GACnn_nn'nnGTC
Cuts at: 1473 2331 1473
Size: 858 2909
EaeI y'GGCC_r
Cuts at: 447 1352 1526 1917 1944 3289 447
Size: 905 174 391 27 1345 925
Fragments arranged by size:
1345 925 905 391 174 27
EagI C'GGCC_G
Cuts at: 1352 1352
Size: 3767
EarI CTCTTCn'nnn_
Cuts at: 125 179 436 1790 2000 125
Size: 54 257 1354 210 1892
Fragments arranged by size:
1892 1354 257 210 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1975 2285 2431 3577 1975
Size: 310 146 1146 2165
Fragments arranged by size:
2165 1146 310 146
EcoICRI GAG'CTC
Cuts at: 779 779
Size: 3767
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 556 670 694 1592 2024 2771 3039 457
Size: 99 114 24 898 432 747 268 1185
Fragments arranged by size:
1185 898 747 432 268 114 99 24
EcoNI CCTnn'n_nnAGG
Cuts at: 538 652 538
Size: 114 3653
EcoO109I rG'GnC_Cy
Cuts at: 482 491 906 482
Size: 9 415 3343
Fragments arranged by size:
3343 415 9
EcoRI G'AATT_C
Cuts at: 771 771
Size: 3767
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1786 1786
Size: 3767
FspI TGC'GCA
Cuts at: 1548 1548
Size: 3767
HaeII r_GCGC'y
Cuts at: 471 1201 1449 2471 3450 471
Size: 730 248 1022 979 788
Fragments arranged by size:
1022 979 788 730 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1609 1639 1641 1671 1609
Size: 30 2 30 3705
Fragments arranged by size:
3705 30 30 2
HincII GTy'rAC
Cuts at: 381 806 381
Size: 425 3342
KasI G'GCGC_C
Cuts at: 1197 1445 1197
Size: 248 3519
KpnI G_GTAC'C
Cuts at: 787 787
Size: 3767
MluI A'CGCG_T
Cuts at: 3200 3200
Size: 3767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2438 2622 2438
Size: 184 3583
MscI TGG'CCA
Cuts at: 449 1528 449
Size: 1079 2688
MslI CAynn'nnrTG
Cuts at: 241 1756 1883 241
Size: 1515 127 2125
Fragments arranged by size:
2125 1515 127
MspA1I CmG'CkG
Cuts at: 717 878 1192 1552 2565 2810 717
Size: 161 314 360 1013 245 1674
Fragments arranged by size:
1674 1013 360 314 245 161
NaeI GCC'GGC
Cuts at: 1948 1948
Size: 3767
NarI GG'CG_CC
Cuts at: 1198 1446 1198
Size: 248 3519
NcoI C'CATG_G
Cuts at: 450 551 450
Size: 101 3666
NgoMIV G'CCGG_C
Cuts at: 1946 1946
Size: 3767
NspI r_CATG'y
Cuts at: 423 713 820 1320 2227 423
Size: 290 107 500 907 1963
Fragments arranged by size:
1963 907 500 290 107
PasI CC'CwG_GG
Cuts at: 373 373
Size: 3767
PciI A'CATG_T
Cuts at: 2223 2223
Size: 3767
PfoI T'CCnGG_A
Cuts at: 533 671 533
Size: 138 3629
PmeI GTTT'AAAC
Cuts at: 766 766
Size: 3767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2906 2938 2906
Size: 32 3735
PspOMI G'GGCC_C
Cuts at: 490 490
Size: 3767
PstI C_TGCA'G
Cuts at: 738 814 1499 738
Size: 76 685 3006
Fragments arranged by size:
3006 685 76
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3767
PvuII CAG'CTG
Cuts at: 1192 1552 1192
Size: 360 3407
RsrII CG'GwC_CG
Cuts at: 1962 1962
Size: 3767
SacI G_AGCT'C
Cuts at: 781 781
Size: 3767
SalI G'TCGA_C
Cuts at: 804 804
Size: 3767
SapI GCTCTTCn'nnn_
Cuts at: 1790 2000 1790
Size: 210 3557
SbfI CC_TGCA'GG
Cuts at: 814 814
Size: 3767
ScaI AGT'ACT
Cuts at: 3284 3284
Size: 3767
SfcI C'TryA_G
Cuts at: 33 734 810 1495 2488 2679 33
Size: 701 76 685 993 191 1121
Fragments arranged by size:
1121 993 701 685 191 76
SfiI GGCCn_nnn'nGGCC
Cuts at: 489 489
Size: 3767
SfoI GGC'GCC
Cuts at: 1199 1447 1199
Size: 248 3519
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3767
SmaI CCC'GGG
Cuts at: 789 789
Size: 3767
SmlI C'TyrA_G
Cuts at: 922 1837 2329 2591 922
Size: 915 492 262 2098
Fragments arranged by size:
2098 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3767
SphI G_CATG'C
Cuts at: 423 713 820 1320 423
Size: 290 107 500 2870
Fragments arranged by size:
2870 500 290 107
SspI AAT'ATT
Cuts at: 286 286
Size: 3767
StyI C'CwwG_G
Cuts at: 450 478 551 901 1255 450
Size: 28 73 350 354 2962
Fragments arranged by size:
2962 354 350 73 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1350 1483 1942 2151 1350
Size: 133 459 209 2966
Fragments arranged by size:
2966 459 209 133
TatI w'GTAC_w
Cuts at: 741 3282 741
Size: 2541 1226
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 667 699 3284 3316 667
Size: 32 2585 32 1118
Fragments arranged by size:
2585 1118 32 32
Tth111I GACn'n_nGTC
Cuts at: 1564 1564
Size: 3767
XbaI T'CTAG_A
Cuts at: 798 798
Size: 3767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 457 1262 457
Size: 805 2962
XmaI C'CCGG_G
Cuts at: 787 787
Size: 3767
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI