(Circular) (Six-base) MAPSORT of: pF1KB8761.seq Check: 2890 from: 1 to: 3731 pF1KB8761 3731 bp With 182 enzymes: SgfI * June 20, 2008 10:29 .. AccI GT'mk_AC Cuts at: 769 769 Size: 3731 Acc65I G'GTAC_C Cuts at: 747 747 Size: 3731 AclI AA'CG_TT Cuts at: 3571 3571 Size: 3731 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 362 452 1556 1988 2735 3003 362 Size: 90 1104 432 747 268 1090 Fragments arranged by size: 1104 1090 747 432 268 90 AflIII A'CryG_T Cuts at: 1083 2187 3164 1083 Size: 1104 977 1650 Fragments arranged by size: 1650 1104 977 AgeI A'CCGG_T Cuts at: 2119 2119 Size: 3731 AlwNI CAG_nnn'CTG Cuts at: 1036 2603 1036 Size: 1567 2164 ApaLI G'TGCA_C Cuts at: 2501 2501 Size: 3731 ApoI r'AATT_y Cuts at: 735 3084 3340 735 Size: 2349 256 1126 Fragments arranged by size: 2349 1126 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3731 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3731 AvaI C'yCGr_G Cuts at: 751 751 Size: 3731 BamHI G'GATC_C Cuts at: 636 756 636 Size: 120 3611 BanI G'GyrC_C Cuts at: 747 1161 1409 1444 747 Size: 414 248 35 3034 Fragments arranged by size: 3034 414 248 35 BanII G_rGCy'C Cuts at: 573 745 1775 573 Size: 172 1030 2529 Fragments arranged by size: 2529 1030 172 BbeI G_GCGC'C Cuts at: 1165 1413 1165 Size: 248 3483 BbsI GAAGACnn'nnnn_ Cuts at: 253 253 Size: 3731 BciVI GTATCCnnnnn_n' Cuts at: 1629 2390 3731 1629 Size: 761 1341 1629 Fragments arranged by size: 1629 1341 761 BclI T'GATC_A Cuts at: 1251 1251 Size: 3731 BglI GCCn_nnn'nGGC Cuts at: 385 385 Size: 3731 BglII A'GATC_T Cuts at: 1246 3283 1246 Size: 2037 1694 BlpI GC'TnA_GC Cuts at: 843 843 Size: 3731 Bme1580I G_kGCm'C Cuts at: 446 1356 1449 2505 446 Size: 910 93 1056 1672 Fragments arranged by size: 1672 1056 910 93 BmrI ACTGGGnnnn_n' Cuts at: 943 1024 1359 3503 943 Size: 81 335 2144 1171 Fragments arranged by size: 2144 1171 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2098 2098 Size: 3731 Bpu10I CC'TnA_GC Cuts at: 572 583 572 Size: 11 3720 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 907 1786 2278 2576 907 Size: 879 492 298 2062 Fragments arranged by size: 2062 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3435 3435 Size: 3731 BsaAI yAC'GTr Cuts at: 1084 1084 Size: 3731 BsaBI GATnn'nnATC Cuts at: 6 1245 3282 6 Size: 1239 2037 455 Fragments arranged by size: 2037 1239 455 BsaHI Gr'CG_yC Cuts at: 1162 1410 2112 3608 1162 Size: 248 702 1496 1285 Fragments arranged by size: 1496 1285 702 248 BsaWI w'CCGG_w Cuts at: 920 1441 2119 2393 2540 2951 920 Size: 521 678 274 147 411 1700 Fragments arranged by size: 1700 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 550 592 550 Size: 42 3689 BseYI C'CCAG_C Cuts at: 1156 1914 2491 2987 1156 Size: 758 577 496 1900 Fragments arranged by size: 1900 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1273 1319 2527 104 Size: 1169 46 1208 1308 Fragments arranged by size: 1308 1208 1169 46 BsiHKAI G_wGCw'C Cuts at: 745 1523 1713 2505 745 Size: 778 190 792 1971 Fragments arranged by size: 1971 792 778 190 BsmI GAATG_Cn' Cuts at: 259 259 Size: 3731 Bsp1286I G_dGCh'C Cuts at: 446 573 745 1356 1449 1523 1713 1775 2505 Size: 127 172 611 93 74 190 62 730 Cuts at: 2505 446 Size: 1672 Fragments arranged by size: 1672 730 611 190 172 127 93 74 62 BspEI T'CCGG_A Cuts at: 2951 2951 Size: 3731 BspHI T'CATG_A Cuts at: 2907 2907 Size: 3731 BspMI ACCTGCnnnn'nnnn_ Cuts at: 781 995 1297 1678 781 Size: 214 302 381 2834 Fragments arranged by size: 2834 381 302 214 BsrBI CCG'CTC Cuts at: 50 2023 2077 3561 3726 50 Size: 1973 54 1484 165 55 Fragments arranged by size: 1973 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1643 1643 Size: 3731 BsrFI r'CCGG_y Cuts at: 1729 1910 2119 1729 Size: 181 209 3341 Fragments arranged by size: 3341 209 181 BsrGI T'GTAC_A Cuts at: 630 630 Size: 3731 BssSI C'ACGA_G Cuts at: 2002 2360 2002 Size: 358 3373 BstAPI GCAn_nnn'nTGC Cuts at: 1108 1108 Size: 3731 BstBI TT'CG_AA Cuts at: 2092 2092 Size: 3731 BstYI r'GATC_y Cuts at: 1 636 756 1223 1246 1581 1827 2839 2925 Size: 635 120 467 23 335 246 1012 86 Cuts at: 2925 2937 3283 1 Size: 12 346 449 Fragments arranged by size: 1012 635 467 449 346 335 246 120 86 23 12 BtgI C'CryG_G Cuts at: 107 406 107 Size: 299 3432 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1681 1862 2025 1681 Size: 181 163 3387 Fragments arranged by size: 3387 181 163 BtsI GCAGTG_nn' Cuts at: 537 974 537 Size: 437 3294 DraI TTT'AAA Cuts at: 730 730 Size: 3731 DraIII CAC_nnn'GTG Cuts at: 364 364 Size: 3731 DrdI GACnn_nn'nnGTC Cuts at: 1437 2295 1437 Size: 858 2873 EaeI y'GGCC_r Cuts at: 1316 1490 1881 1908 3253 1316 Size: 174 391 27 1345 1794 Fragments arranged by size: 1794 1345 391 174 27 EagI C'GGCC_G Cuts at: 1316 1316 Size: 3731 EarI CTCTTCn'nnn_ Cuts at: 1754 1964 1754 Size: 210 3521 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 698 1939 2249 2395 3541 698 Size: 1241 310 146 1146 888 Fragments arranged by size: 1241 1146 888 310 146 EcoICRI GAG'CTC Cuts at: 743 743 Size: 3731 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 362 452 1556 1988 2735 3003 362 Size: 90 1104 432 747 268 1090 Fragments arranged by size: 1104 1090 747 432 268 90 EcoO109I rG'GnC_Cy Cuts at: 870 870 Size: 3731 EcoRI G'AATT_C Cuts at: 735 735 Size: 3731 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1750 1750 Size: 3731 FspI TGC'GCA Cuts at: 1512 1512 Size: 3731 HaeII r_GCGC'y Cuts at: 426 1165 1413 2435 3414 426 Size: 739 248 1022 979 743 Fragments arranged by size: 1022 979 743 739 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1573 1603 1605 1635 1573 Size: 30 2 30 3669 Fragments arranged by size: 3669 30 30 2 HincII GTy'rAC Cuts at: 770 770 Size: 3731 KasI G'GCGC_C Cuts at: 1161 1409 1161 Size: 248 3483 KpnI G_GTAC'C Cuts at: 751 751 Size: 3731 MluI A'CGCG_T Cuts at: 3164 3164 Size: 3731 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2402 2586 2402 Size: 184 3547 MscI TGG'CCA Cuts at: 1492 1492 Size: 3731 MslI CAynn'nnrTG Cuts at: 1720 1847 1720 Size: 127 3604 MspA1I CmG'CkG Cuts at: 408 842 1156 1516 2529 2774 408 Size: 434 314 360 1013 245 1365 Fragments arranged by size: 1365 1013 434 360 314 245 NaeI GCC'GGC Cuts at: 1912 1912 Size: 3731 NarI GG'CG_CC Cuts at: 1162 1410 1162 Size: 248 3483 NcoI C'CATG_G Cuts at: 107 107 Size: 3731 NdeI CA'TA_TG Cuts at: 720 720 Size: 3731 NgoMIV G'CCGG_C Cuts at: 1910 1910 Size: 3731 NspI r_CATG'y Cuts at: 784 1284 2191 784 Size: 500 907 2324 Fragments arranged by size: 2324 907 500 PciI A'CATG_T Cuts at: 2187 2187 Size: 3731 PmeI GTTT'AAAC Cuts at: 730 730 Size: 3731 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2870 2902 2870 Size: 32 3699 PstI C_TGCA'G Cuts at: 778 1463 778 Size: 685 3046 PvuI CG_AT'CG Cuts at: 104 104 Size: 3731 PvuII CAG'CTG Cuts at: 1156 1516 1156 Size: 360 3371 RsrII CG'GwC_CG Cuts at: 1926 1926 Size: 3731 SacI G_AGCT'C Cuts at: 745 745 Size: 3731 SacII CC_GC'GG Cuts at: 409 409 Size: 3731 SalI G'TCGA_C Cuts at: 768 768 Size: 3731 SapI GCTCTTCn'nnn_ Cuts at: 1754 1964 1754 Size: 210 3521 SbfI CC_TGCA'GG Cuts at: 778 778 Size: 3731 ScaI AGT'ACT Cuts at: 3248 3248 Size: 3731 SfcI C'TryA_G Cuts at: 33 774 1459 2452 2643 33 Size: 741 685 993 191 1121 Fragments arranged by size: 1121 993 741 685 191 SfoI GGC'GCC Cuts at: 1163 1411 1163 Size: 248 3483 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3731 SmaI CCC'GGG Cuts at: 753 753 Size: 3731 SmlI C'TyrA_G Cuts at: 886 1801 2293 2555 886 Size: 915 492 262 2062 Fragments arranged by size: 2062 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3731 SphI G_CATG'C Cuts at: 784 1284 784 Size: 500 3231 StyI C'CwwG_G Cuts at: 107 491 649 865 1219 107 Size: 384 158 216 354 2619 Fragments arranged by size: 2619 384 354 216 158 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 592 1314 1447 1906 2115 592 Size: 722 133 459 209 2208 Fragments arranged by size: 2208 722 459 209 133 TatI w'GTAC_w Cuts at: 193 630 3246 193 Size: 437 2616 678 Fragments arranged by size: 2616 678 437 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3248 3280 3248 Size: 32 3699 Tth111I GACn'n_nGTC Cuts at: 1528 1528 Size: 3731 XbaI T'CTAG_A Cuts at: 762 762 Size: 3731 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1226 1226 Size: 3731 XmaI C'CCGG_G Cuts at: 751 751 Size: 3731 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BpmI BsaXI BsgI BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI