Restriction map for pF1KB8761
 (Circular) (Six-base) MAPSORT of: pF1KB8761.seq  Check: 2890  from: 1  to: 3731



pF1KB8761  3731 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:29  ..

AccI GT'mk_AC

Cuts at:    769     769 
   Size:       3731
 
Acc65I G'GTAC_C

Cuts at:    747     747 
   Size:       3731
 
AclI AA'CG_TT

Cuts at:   3571    3571 
   Size:       3731
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    362     452    1556    1988    2735    3003     362 
   Size:         90    1104     432     747     268    1090

  Fragments arranged by size: 

                1104    1090     747     432     268      90
 
AflIII A'CryG_T

Cuts at:   1083    2187    3164    1083 
   Size:       1104     977    1650

  Fragments arranged by size: 

                1650    1104     977
 
AgeI A'CCGG_T

Cuts at:   2119    2119 
   Size:       3731
 
AlwNI CAG_nnn'CTG

Cuts at:   1036    2603    1036 
   Size:       1567    2164
 
ApaLI G'TGCA_C

Cuts at:   2501    2501 
   Size:       3731
 
ApoI r'AATT_y

Cuts at:    735    3084    3340     735 
   Size:       2349     256    1126

  Fragments arranged by size: 

                2349    1126     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3731
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3731
 
AvaI C'yCGr_G

Cuts at:    751     751 
   Size:       3731
 
BamHI G'GATC_C

Cuts at:    636     756     636 
   Size:        120    3611
 
BanI G'GyrC_C

Cuts at:    747    1161    1409    1444     747 
   Size:        414     248      35    3034

  Fragments arranged by size: 

                3034     414     248      35
 
BanII G_rGCy'C

Cuts at:    573     745    1775     573 
   Size:        172    1030    2529

  Fragments arranged by size: 

                2529    1030     172
 
BbeI G_GCGC'C

Cuts at:   1165    1413    1165 
   Size:        248    3483
 
BbsI GAAGACnn'nnnn_

Cuts at:    253     253 
   Size:       3731
 
BciVI GTATCCnnnnn_n'

Cuts at:   1629    2390    3731    1629 
   Size:        761    1341    1629

  Fragments arranged by size: 

                1629    1341     761
 
BclI T'GATC_A

Cuts at:   1251    1251 
   Size:       3731
 
BglI GCCn_nnn'nGGC

Cuts at:    385     385 
   Size:       3731
 
BglII A'GATC_T

Cuts at:   1246    3283    1246 
   Size:       2037    1694
 
BlpI GC'TnA_GC

Cuts at:    843     843 
   Size:       3731
 
Bme1580I G_kGCm'C

Cuts at:    446    1356    1449    2505     446 
   Size:        910      93    1056    1672

  Fragments arranged by size: 

                1672    1056     910      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    943    1024    1359    3503     943 
   Size:         81     335    2144    1171

  Fragments arranged by size: 

                2144    1171     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2098    2098 
   Size:       3731
 
Bpu10I CC'TnA_GC

Cuts at:    572     583     572 
   Size:         11    3720
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    907    1786    2278    2576     907 
   Size:        879     492     298    2062

  Fragments arranged by size: 

                2062     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3435    3435 
   Size:       3731
 
BsaAI yAC'GTr

Cuts at:   1084    1084 
   Size:       3731
 
BsaBI GATnn'nnATC

Cuts at:      6    1245    3282       6 
   Size:       1239    2037     455

  Fragments arranged by size: 

                2037    1239     455
 
BsaHI Gr'CG_yC

Cuts at:   1162    1410    2112    3608    1162 
   Size:        248     702    1496    1285

  Fragments arranged by size: 

                1496    1285     702     248
 
BsaWI w'CCGG_w

Cuts at:    920    1441    2119    2393    2540    2951     920 
   Size:        521     678     274     147     411    1700

  Fragments arranged by size: 

                1700     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    550     592     550 
   Size:         42    3689
 
BseYI C'CCAG_C

Cuts at:   1156    1914    2491    2987    1156 
   Size:        758     577     496    1900

  Fragments arranged by size: 

                1900     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1273    1319    2527     104 
   Size:       1169      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1169      46
 
BsiHKAI G_wGCw'C

Cuts at:    745    1523    1713    2505     745 
   Size:        778     190     792    1971

  Fragments arranged by size: 

                1971     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    259     259 
   Size:       3731
 
Bsp1286I G_dGCh'C

Cuts at:    446     573     745    1356    1449    1523    1713    1775    2505 
   Size:        127     172     611      93      74     190      62     730

Cuts at:   2505     446 
   Size:       1672

  Fragments arranged by size: 

                1672     730     611     190     172     127      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   2951    2951 
   Size:       3731
 
BspHI T'CATG_A

Cuts at:   2907    2907 
   Size:       3731
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    781     995    1297    1678     781 
   Size:        214     302     381    2834

  Fragments arranged by size: 

                2834     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2023    2077    3561    3726      50 
   Size:       1973      54    1484     165      55

  Fragments arranged by size: 

                1973    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1643    1643 
   Size:       3731
 
BsrFI r'CCGG_y

Cuts at:   1729    1910    2119    1729 
   Size:        181     209    3341

  Fragments arranged by size: 

                3341     209     181
 
BsrGI T'GTAC_A

Cuts at:    630     630 
   Size:       3731
 
BssSI C'ACGA_G

Cuts at:   2002    2360    2002 
   Size:        358    3373
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1108    1108 
   Size:       3731
 
BstBI TT'CG_AA

Cuts at:   2092    2092 
   Size:       3731
 
BstYI r'GATC_y

Cuts at:      1     636     756    1223    1246    1581    1827    2839    2925 
   Size:        635     120     467      23     335     246    1012      86

Cuts at:   2925    2937    3283       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     635     467     449     346     335     246     120
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     406     107 
   Size:        299    3432
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1681    1862    2025    1681 
   Size:        181     163    3387

  Fragments arranged by size: 

                3387     181     163
 
BtsI GCAGTG_nn'

Cuts at:    537     974     537 
   Size:        437    3294
 
DraI TTT'AAA

Cuts at:    730     730 
   Size:       3731
 
DraIII CAC_nnn'GTG

Cuts at:    364     364 
   Size:       3731
 
DrdI GACnn_nn'nnGTC

Cuts at:   1437    2295    1437 
   Size:        858    2873
 
EaeI y'GGCC_r

Cuts at:   1316    1490    1881    1908    3253    1316 
   Size:        174     391      27    1345    1794

  Fragments arranged by size: 

                1794    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1316    1316 
   Size:       3731
 
EarI CTCTTCn'nnn_

Cuts at:   1754    1964    1754 
   Size:        210    3521
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    698    1939    2249    2395    3541     698 
   Size:       1241     310     146    1146     888

  Fragments arranged by size: 

                1241    1146     888     310     146
 
EcoICRI GAG'CTC

Cuts at:    743     743 
   Size:       3731
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    362     452    1556    1988    2735    3003     362 
   Size:         90    1104     432     747     268    1090

  Fragments arranged by size: 

                1104    1090     747     432     268      90
 
EcoO109I rG'GnC_Cy

Cuts at:    870     870 
   Size:       3731
 
EcoRI G'AATT_C

Cuts at:    735     735 
   Size:       3731
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1750    1750 
   Size:       3731
 
FspI TGC'GCA

Cuts at:   1512    1512 
   Size:       3731
 
HaeII r_GCGC'y

Cuts at:    426    1165    1413    2435    3414     426 
   Size:        739     248    1022     979     743

  Fragments arranged by size: 

                1022     979     743     739     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1573    1603    1605    1635    1573 
   Size:         30       2      30    3669

  Fragments arranged by size: 

                3669      30      30       2
 
HincII GTy'rAC

Cuts at:    770     770 
   Size:       3731
 
KasI G'GCGC_C

Cuts at:   1161    1409    1161 
   Size:        248    3483
 
KpnI G_GTAC'C

Cuts at:    751     751 
   Size:       3731
 
MluI A'CGCG_T

Cuts at:   3164    3164 
   Size:       3731
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2402    2586    2402 
   Size:        184    3547
 
MscI TGG'CCA

Cuts at:   1492    1492 
   Size:       3731
 
MslI CAynn'nnrTG

Cuts at:   1720    1847    1720 
   Size:        127    3604
 
MspA1I CmG'CkG

Cuts at:    408     842    1156    1516    2529    2774     408 
   Size:        434     314     360    1013     245    1365

  Fragments arranged by size: 

                1365    1013     434     360     314     245
 
NaeI GCC'GGC

Cuts at:   1912    1912 
   Size:       3731
 
NarI GG'CG_CC

Cuts at:   1162    1410    1162 
   Size:        248    3483
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3731
 
NdeI CA'TA_TG

Cuts at:    720     720 
   Size:       3731
 
NgoMIV G'CCGG_C

Cuts at:   1910    1910 
   Size:       3731
 
NspI r_CATG'y

Cuts at:    784    1284    2191     784 
   Size:        500     907    2324

  Fragments arranged by size: 

                2324     907     500
 
PciI A'CATG_T

Cuts at:   2187    2187 
   Size:       3731
 
PmeI GTTT'AAAC

Cuts at:    730     730 
   Size:       3731
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2870    2902    2870 
   Size:         32    3699
 
PstI C_TGCA'G

Cuts at:    778    1463     778 
   Size:        685    3046
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3731
 
PvuII CAG'CTG

Cuts at:   1156    1516    1156 
   Size:        360    3371
 
RsrII CG'GwC_CG

Cuts at:   1926    1926 
   Size:       3731
 
SacI G_AGCT'C

Cuts at:    745     745 
   Size:       3731
 
SacII CC_GC'GG

Cuts at:    409     409 
   Size:       3731
 
SalI G'TCGA_C

Cuts at:    768     768 
   Size:       3731
 
SapI GCTCTTCn'nnn_

Cuts at:   1754    1964    1754 
   Size:        210    3521
 
SbfI CC_TGCA'GG

Cuts at:    778     778 
   Size:       3731
 
ScaI AGT'ACT

Cuts at:   3248    3248 
   Size:       3731
 
SfcI C'TryA_G

Cuts at:     33     774    1459    2452    2643      33 
   Size:        741     685     993     191    1121

  Fragments arranged by size: 

                1121     993     741     685     191
 
SfoI GGC'GCC

Cuts at:   1163    1411    1163 
   Size:        248    3483
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3731
 
SmaI CCC'GGG

Cuts at:    753     753 
   Size:       3731
 
SmlI C'TyrA_G

Cuts at:    886    1801    2293    2555     886 
   Size:        915     492     262    2062

  Fragments arranged by size: 

                2062     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3731
 
SphI G_CATG'C

Cuts at:    784    1284     784 
   Size:        500    3231
 
StyI C'CwwG_G

Cuts at:    107     491     649     865    1219     107 
   Size:        384     158     216     354    2619

  Fragments arranged by size: 

                2619     384     354     216     158
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    592    1314    1447    1906    2115     592 
   Size:        722     133     459     209    2208

  Fragments arranged by size: 

                2208     722     459     209     133
 
TatI w'GTAC_w

Cuts at:    193     630    3246     193 
   Size:        437    2616     678

  Fragments arranged by size: 

                2616     678     437
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3248    3280    3248 
   Size:         32    3699
 
Tth111I GACn'n_nGTC

Cuts at:   1528    1528 
   Size:       3731
 
XbaI T'CTAG_A

Cuts at:    762     762 
   Size:       3731
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1226    1226 
   Size:       3731
 
XmaI C'CCGG_G

Cuts at:    751     751 
   Size:       3731
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NspI     PciI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI
     BmtI     BpmI    BsaXI     BsgI    BsiWI    BsmBI   BssHII   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI    CspCI    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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