Restriction map for
pF1KB8760
(Circular) (Six-base) MAPSORT of: pF1KB8760.seq Check: 6358 from: 1 to: 3722
pF1KB8760 3722 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 214 214
Size: 3722
AccI GT'mk_AC
Cuts at: 760 760
Size: 3722
Acc65I G'GTAC_C
Cuts at: 738 738
Size: 3722
AclI AA'CG_TT
Cuts at: 3562 3562
Size: 3722
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1547 1979 2726 2994 1547
Size: 432 747 268 2275
Fragments arranged by size:
2275 747 432 268
AfeI AGC'GCT
Cuts at: 694 694
Size: 3722
AflIII A'CryG_T
Cuts at: 674 1074 2178 3155 674
Size: 400 1104 977 1241
Fragments arranged by size:
1241 1104 977 400
AgeI A'CCGG_T
Cuts at: 2110 2110
Size: 3722
AleI CACnn'nnGTG
Cuts at: 502 502
Size: 3722
AlwNI CAG_nnn'CTG
Cuts at: 531 1027 2594 531
Size: 496 1567 1659
Fragments arranged by size:
1659 1567 496
ApaLI G'TGCA_C
Cuts at: 615 2492 615
Size: 1877 1845
ApoI r'AATT_y
Cuts at: 726 3075 3331 726
Size: 2349 256 1117
Fragments arranged by size:
2349 1117 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3722
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3722
AvaI C'yCGr_G
Cuts at: 475 742 475
Size: 267 3455
BamHI G'GATC_C
Cuts at: 747 747
Size: 3722
BanI G'GyrC_C
Cuts at: 257 266 738 1152 1400 1435 257
Size: 9 472 414 248 35 2544
Fragments arranged by size:
2544 472 414 248 35 9
BanII G_rGCy'C
Cuts at: 546 736 1766 546
Size: 190 1030 2502
Fragments arranged by size:
2502 1030 190
BbeI G_GCGC'C
Cuts at: 1156 1404 1156
Size: 248 3474
BbsI GAAGACnn'nnnn_
Cuts at: 369 639 369
Size: 270 3452
BciVI GTATCCnnnnn_n'
Cuts at: 1620 2381 3722 1620
Size: 761 1341 1620
Fragments arranged by size:
1620 1341 761
BclI T'GATC_A
Cuts at: 1242 1242
Size: 3722
BglII A'GATC_T
Cuts at: 146 1237 3274 146
Size: 1091 2037 594
Fragments arranged by size:
2037 1091 594
BlpI GC'TnA_GC
Cuts at: 834 834
Size: 3722
Bme1580I G_kGCm'C
Cuts at: 262 619 1347 1440 2496 262
Size: 357 728 93 1056 1488
Fragments arranged by size:
1488 1056 728 357 93
BmrI ACTGGGnnnn_n'
Cuts at: 934 1015 1350 3494 934
Size: 81 335 2144 1162
Fragments arranged by size:
2144 1162 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2089 2089
Size: 3722
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 898 1777 2269 2567 898
Size: 879 492 298 2053
Fragments arranged by size:
2053 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3426 3426
Size: 3722
BsaAI yAC'GTr
Cuts at: 1075 1075
Size: 3722
BsaBI GATnn'nnATC
Cuts at: 6 1236 3273 6
Size: 1230 2037 455
Fragments arranged by size:
2037 1230 455
BsaHI Gr'CG_yC
Cuts at: 1153 1401 2103 3599 1153
Size: 248 702 1496 1276
Fragments arranged by size:
1496 1276 702 248
BsaWI w'CCGG_w
Cuts at: 911 1432 2110 2384 2531 2942 911
Size: 521 678 274 147 411 1691
Fragments arranged by size:
1691 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 103 133 103
Size: 30 3692
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 577 580 643 577
Size: 3 63 3656
Fragments arranged by size:
3656 63 3
BseYI C'CCAG_C
Cuts at: 391 1147 1905 2482 2978 391
Size: 756 758 577 496 1135
Fragments arranged by size:
1135 758 756 577 496
BsiEI CG_ry'CG
Cuts at: 104 1264 1310 2518 104
Size: 1160 46 1208 1308
Fragments arranged by size:
1308 1208 1160 46
BsiHKAI G_wGCw'C
Cuts at: 619 736 1514 1704 2496 619
Size: 117 778 190 792 1845
Fragments arranged by size:
1845 792 778 190 117
BsmBI CGTCTCn'nnnn_
Cuts at: 106 106
Size: 3722
Bsp1286I G_dGCh'C
Cuts at: 262 546 619 736 1347 1440 1514 1704 1766
Size: 284 73 117 611 93 74 190 62
Cuts at: 1766 2496 262
Size: 730 1488
Fragments arranged by size:
1488 730 611 284 190 117 93 74
73 62
BspEI T'CCGG_A
Cuts at: 2942 2942
Size: 3722
BspHI T'CATG_A
Cuts at: 2898 2898
Size: 3722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 214 772 986 1288 1669 214
Size: 558 214 302 381 2267
Fragments arranged by size:
2267 558 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2014 2068 3552 3717 50
Size: 1964 54 1484 165 55
Fragments arranged by size:
1964 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 402 1634 402
Size: 1232 2490
BsrFI r'CCGG_y
Cuts at: 1720 1901 2110 1720
Size: 181 209 3332
Fragments arranged by size:
3332 209 181
BssSI C'ACGA_G
Cuts at: 1993 2351 1993
Size: 358 3364
BstAPI GCAn_nnn'nTGC
Cuts at: 401 531 1099 401
Size: 130 568 3024
Fragments arranged by size:
3024 568 130
BstBI TT'CG_AA
Cuts at: 2083 2083
Size: 3722
BstEII G'GTnAC_C
Cuts at: 504 504
Size: 3722
BstXI CCAn_nnnn'nTGG
Cuts at: 189 189
Size: 3722
BstYI r'GATC_y
Cuts at: 1 146 747 1214 1237 1572 1818 2830 2916
Size: 145 601 467 23 335 246 1012 86
Cuts at: 2916 2928 3274 1
Size: 12 346 449
Fragments arranged by size:
1012 601 467 449 346 335 246 145
86 23 12
BtgI C'CryG_G
Cuts at: 107 270 500 107
Size: 163 230 3329
Fragments arranged by size:
3329 230 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 327 1672 1853 2016 327
Size: 1345 181 163 2033
Fragments arranged by size:
2033 1345 181 163
BtsI GCAGTG_nn'
Cuts at: 965 965
Size: 3722
DraI TTT'AAA
Cuts at: 721 721
Size: 3722
DrdI GACnn_nn'nnGTC
Cuts at: 328 1428 2286 328
Size: 1100 858 1764
Fragments arranged by size:
1764 1100 858
EaeI y'GGCC_r
Cuts at: 1307 1481 1872 1899 3244 1307
Size: 174 391 27 1345 1785
Fragments arranged by size:
1785 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1307 1307
Size: 3722
EarI CTCTTCn'nnn_
Cuts at: 1745 1955 1745
Size: 210 3512
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1930 2240 2386 3532 1930
Size: 310 146 1146 2120
Fragments arranged by size:
2120 1146 310 146
EcoICRI GAG'CTC
Cuts at: 734 734
Size: 3722
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1547 1979 2726 2994 1547
Size: 432 747 268 2275
Fragments arranged by size:
2275 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 533 533
Size: 3722
EcoO109I rG'GnC_Cy
Cuts at: 330 861 330
Size: 531 3191
EcoRI G'AATT_C
Cuts at: 726 726
Size: 3722
EcoRV GAT'ATC
Cuts at: 707 707
Size: 3722
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1741 1741
Size: 3722
FspI TGC'GCA
Cuts at: 1503 1503
Size: 3722
HaeII r_GCGC'y
Cuts at: 696 1156 1404 2426 3405 696
Size: 460 248 1022 979 1013
Fragments arranged by size:
1022 1013 979 460 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 1564 1594 1596 1626 103
Size: 32 1429 30 2 30 2199
Fragments arranged by size:
2199 1429 32 30 30 2
HincII GTy'rAC
Cuts at: 761 761
Size: 3722
KasI G'GCGC_C
Cuts at: 1152 1400 1152
Size: 248 3474
KpnI G_GTAC'C
Cuts at: 742 742
Size: 3722
MluI A'CGCG_T
Cuts at: 3155 3155
Size: 3722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2393 2577 2393
Size: 184 3538
MscI TGG'CCA
Cuts at: 1483 1483
Size: 3722
MslI CAynn'nnrTG
Cuts at: 502 628 1711 1838 502
Size: 126 1083 127 2386
Fragments arranged by size:
2386 1083 127 126
MspA1I CmG'CkG
Cuts at: 272 833 1147 1507 2520 2765 272
Size: 561 314 360 1013 245 1229
Fragments arranged by size:
1229 1013 561 360 314 245
NaeI GCC'GGC
Cuts at: 1903 1903
Size: 3722
NarI GG'CG_CC
Cuts at: 1153 1401 1153
Size: 248 3474
NcoI C'CATG_G
Cuts at: 107 107
Size: 3722
NgoMIV G'CCGG_C
Cuts at: 1901 1901
Size: 3722
NspI r_CATG'y
Cuts at: 411 678 775 1275 2182 411
Size: 267 97 500 907 1951
Fragments arranged by size:
1951 907 500 267 97
PciI A'CATG_T
Cuts at: 674 2178 674
Size: 1504 2218
PfoI T'CCnGG_A
Cuts at: 314 314
Size: 3722
PmeI GTTT'AAAC
Cuts at: 721 721
Size: 3722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2861 2893 2861
Size: 32 3690
PpuMI rG'GwC_Cy
Cuts at: 330 330
Size: 3722
PstI C_TGCA'G
Cuts at: 227 769 1454 227
Size: 542 685 2495
Fragments arranged by size:
2495 685 542
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3722
PvuII CAG'CTG
Cuts at: 1147 1507 1147
Size: 360 3362
RsrII CG'GwC_CG
Cuts at: 1917 1917
Size: 3722
SacI G_AGCT'C
Cuts at: 736 736
Size: 3722
SacII CC_GC'GG
Cuts at: 273 273
Size: 3722
SalI G'TCGA_C
Cuts at: 759 759
Size: 3722
SapI GCTCTTCn'nnn_
Cuts at: 1745 1955 1745
Size: 210 3512
SbfI CC_TGCA'GG
Cuts at: 769 769
Size: 3722
ScaI AGT'ACT
Cuts at: 3239 3239
Size: 3722
SexAI A'CCwGG_T
Cuts at: 601 601
Size: 3722
SfcI C'TryA_G
Cuts at: 33 162 223 765 1450 2443 2634 33
Size: 129 61 542 685 993 191 1121
Fragments arranged by size:
1121 993 685 542 191 129 61
SfoI GGC'GCC
Cuts at: 1154 1402 1154
Size: 248 3474
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3722
SmaI CCC'GGG
Cuts at: 477 744 477
Size: 267 3455
SmlI C'TyrA_G
Cuts at: 877 1792 2284 2546 877
Size: 915 492 262 2053
Fragments arranged by size:
2053 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3722
SphI G_CATG'C
Cuts at: 775 1275 775
Size: 500 3222
StyI C'CwwG_G
Cuts at: 107 428 856 1210 107
Size: 321 428 354 2619
Fragments arranged by size:
2619 428 354 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 389 1305 1438 1897 2106 389
Size: 916 133 459 209 2005
Fragments arranged by size:
2005 916 459 209 133
TatI w'GTAC_w
Cuts at: 3237 3237
Size: 3722
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 241 273 3239 3271 241
Size: 32 2966 32 692
Fragments arranged by size:
2966 692 32 32
Tth111I GACn'n_nGTC
Cuts at: 117 1519 117
Size: 1402 2320
XbaI T'CTAG_A
Cuts at: 753 753
Size: 3722
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1217 1217
Size: 3722
XmaI C'CCGG_G
Cuts at: 475 742 475
Size: 267 3455
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI
Bpu10I BsgI BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI DraIII FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI