Restriction map for
pF1KB8748
(Circular) (Six-base) MAPSORT of: pF1KB8748.seq Check: 8736 from: 1 to: 3620
pF1KB8748 3620 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 156 156
Size: 3620
AccI GT'mk_AC
Cuts at: 658 658
Size: 3620
Acc65I G'GTAC_C
Cuts at: 552 636 552
Size: 84 3536
AclI AA'CG_TT
Cuts at: 3460 3460
Size: 3620
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1445 1877 2624 2892 1445
Size: 432 747 268 2173
Fragments arranged by size:
2173 747 432 268
AflIII A'CryG_T
Cuts at: 972 2076 3053 972
Size: 1104 977 1539
Fragments arranged by size:
1539 1104 977
AgeI A'CCGG_T
Cuts at: 2008 2008
Size: 3620
AlwNI CAG_nnn'CTG
Cuts at: 925 2492 925
Size: 1567 2053
ApaLI G'TGCA_C
Cuts at: 2390 2390
Size: 3620
ApoI r'AATT_y
Cuts at: 624 2973 3229 624
Size: 2349 256 1015
Fragments arranged by size:
2349 1015 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3620
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3620
AvaI C'yCGr_G
Cuts at: 640 640
Size: 3620
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 536 569 536
Size: 33 3587
BamHI G'GATC_C
Cuts at: 645 645
Size: 3620
BanI G'GyrC_C
Cuts at: 493 552 636 1050 1298 1333 493
Size: 59 84 414 248 35 2780
Fragments arranged by size:
2780 414 248 84 59 35
BanII G_rGCy'C
Cuts at: 634 1664 634
Size: 1030 2590
BbeI G_GCGC'C
Cuts at: 1054 1302 1054
Size: 248 3372
BbsI GAAGACnn'nnnn_
Cuts at: 607 607
Size: 3620
BbvCI CC'TCA_GC
Cuts at: 149 149
Size: 3620
BciVI GTATCCnnnnn_n'
Cuts at: 511 1518 2279 3620 511
Size: 1007 761 1341 511
Fragments arranged by size:
1341 1007 761 511
BclI T'GATC_A
Cuts at: 1140 1140
Size: 3620
BglII A'GATC_T
Cuts at: 1135 3172 1135
Size: 2037 1583
BlpI GC'TnA_GC
Cuts at: 593 732 593
Size: 139 3481
Bme1580I G_kGCm'C
Cuts at: 1245 1338 2394 1245
Size: 93 1056 2471
Fragments arranged by size:
2471 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 832 913 1248 3392 832
Size: 81 335 2144 1060
Fragments arranged by size:
2144 1060 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1987 1987
Size: 3620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 566
Size: 3620
Bpu10I CC'TnA_GC
Cuts at: 149 293 317 341 365 389 413 485 509
Size: 144 24 24 24 24 24 72 24
Cuts at: 509 149
Size: 3260
Fragments arranged by size:
3260 144 72 24 24 24 24 24
24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 796 1675 2167 2465 796
Size: 879 492 298 1951
Fragments arranged by size:
1951 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3324 3324
Size: 3620
BsaAI yAC'GTr
Cuts at: 973 973
Size: 3620
BsaBI GATnn'nnATC
Cuts at: 6 1134 3171 6
Size: 1128 2037 455
Fragments arranged by size:
2037 1128 455
BsaHI Gr'CG_yC
Cuts at: 1051 1299 2001 3497 1051
Size: 248 702 1496 1174
Fragments arranged by size:
1496 1174 702 248
BsaWI w'CCGG_w
Cuts at: 809 1330 2008 2282 2429 2840 809
Size: 521 678 274 147 411 1589
Fragments arranged by size:
1589 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 564 594 564
Size: 30 3590
BseYI C'CCAG_C
Cuts at: 178 588 1045 1803 2380 2876 178
Size: 410 457 758 577 496 922
Fragments arranged by size:
922 758 577 496 457 410
BsiEI CG_ry'CG
Cuts at: 104 1162 1208 2416 104
Size: 1058 46 1208 1308
Fragments arranged by size:
1308 1208 1058 46
BsiHKAI G_wGCw'C
Cuts at: 634 1412 1602 2394 634
Size: 778 190 792 1860
Fragments arranged by size:
1860 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 634 1245 1338 1412 1602 1664 2394 634
Size: 611 93 74 190 62 730 1860
Fragments arranged by size:
1860 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2840 2840
Size: 3620
BspHI T'CATG_A
Cuts at: 2796 2796
Size: 3620
BspMI ACCTGCnnnn'nnnn_
Cuts at: 156 670 884 1186 1567 156
Size: 514 214 302 381 2209
Fragments arranged by size:
2209 514 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1912 1966 3450 3615 50
Size: 1862 54 1484 165 55
Fragments arranged by size:
1862 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1532 1532
Size: 3620
BsrFI r'CCGG_y
Cuts at: 1618 1799 2008 1618
Size: 181 209 3230
Fragments arranged by size:
3230 209 181
BssSI C'ACGA_G
Cuts at: 1891 2249 1891
Size: 358 3262
BstAPI GCAn_nnn'nTGC
Cuts at: 997 997
Size: 3620
BstBI TT'CG_AA
Cuts at: 1981 1981
Size: 3620
BstYI r'GATC_y
Cuts at: 1 645 1112 1135 1470 1716 2728 2814 2826
Size: 644 467 23 335 246 1012 86 12
Cuts at: 2826 3172 1
Size: 346 449
Fragments arranged by size:
1012 644 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 194 194
Size: 3620
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1570 1751 1914 1570
Size: 181 163 3276
Fragments arranged by size:
3276 181 163
BtsI GCAGTG_nn'
Cuts at: 863 863
Size: 3620
DraI TTT'AAA
Cuts at: 619 619
Size: 3620
DrdI GACnn_nn'nnGTC
Cuts at: 1326 2184 1326
Size: 858 2762
EaeI y'GGCC_r
Cuts at: 1205 1379 1770 1797 3142 1205
Size: 174 391 27 1345 1683
Fragments arranged by size:
1683 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1205 1205
Size: 3620
EarI CTCTTCn'nnn_
Cuts at: 1643 1853 1643
Size: 210 3410
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1828 2138 2284 3430 1828
Size: 310 146 1146 2018
Fragments arranged by size:
2018 1146 310 146
EcoICRI GAG'CTC
Cuts at: 632 632
Size: 3620
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 1445 1877 2624 2892 566
Size: 879 432 747 268 1294
Fragments arranged by size:
1294 879 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 296 320 344 368 392 416 440 464 488
Size: 24 24 24 24 24 24 24 24
Cuts at: 488 512 536 296
Size: 24 24 3380
Fragments arranged by size:
3380 24 24 24 24 24 24 24
24 24 24
EcoO109I rG'GnC_Cy
Cuts at: 288 312 336 360 384 408 432 456 759
Size: 24 24 24 24 24 24 24 303
Cuts at: 759 288
Size: 3149
Fragments arranged by size:
3149 303 24 24 24 24 24 24
24
EcoRI G'AATT_C
Cuts at: 624 624
Size: 3620
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 150 1639 150
Size: 1489 2131
FspI TGC'GCA
Cuts at: 1401 1401
Size: 3620
HaeII r_GCGC'y
Cuts at: 1054 1302 2324 3303 1054
Size: 248 1022 979 1371
Fragments arranged by size:
1371 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1462 1492 1494 1524 1462
Size: 30 2 30 3558
Fragments arranged by size:
3558 30 30 2
HincII GTy'rAC
Cuts at: 659 659
Size: 3620
KasI G'GCGC_C
Cuts at: 1050 1298 1050
Size: 248 3372
KpnI G_GTAC'C
Cuts at: 556 640 556
Size: 84 3536
MluI A'CGCG_T
Cuts at: 3053 3053
Size: 3620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2291 2475 2291
Size: 184 3436
MscI TGG'CCA
Cuts at: 1381 1381
Size: 3620
MslI CAynn'nnrTG
Cuts at: 1609 1736 1609
Size: 127 3493
MspA1I CmG'CkG
Cuts at: 731 1045 1405 2418 2663 731
Size: 314 360 1013 245 1688
Fragments arranged by size:
1688 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1801 1801
Size: 3620
NarI GG'CG_CC
Cuts at: 1051 1299 1051
Size: 248 3372
NgoMIV G'CCGG_C
Cuts at: 1799 1799
Size: 3620
NspI r_CATG'y
Cuts at: 673 1173 2080 673
Size: 500 907 2213
Fragments arranged by size:
2213 907 500
PciI A'CATG_T
Cuts at: 2076 2076
Size: 3620
PflMI CCAn_nnn'nTGG
Cuts at: 250 250
Size: 3620
PmeI GTTT'AAAC
Cuts at: 619 619
Size: 3620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2759 2791 2759
Size: 32 3588
PpuMI rG'GwC_Cy
Cuts at: 288 312 336 360 384 408 432 456 288
Size: 24 24 24 24 24 24 24 3452
Fragments arranged by size:
3452 24 24 24 24 24 24 24
PstI C_TGCA'G
Cuts at: 667 1352 667
Size: 685 2935
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3620
PvuII CAG'CTG
Cuts at: 1045 1405 1045
Size: 360 3260
RsrII CG'GwC_CG
Cuts at: 1815 1815
Size: 3620
SacI G_AGCT'C
Cuts at: 634 634
Size: 3620
SalI G'TCGA_C
Cuts at: 657 657
Size: 3620
SapI GCTCTTCn'nnn_
Cuts at: 1643 1853 1643
Size: 210 3410
SbfI CC_TGCA'GG
Cuts at: 667 667
Size: 3620
ScaI AGT'ACT
Cuts at: 3137 3137
Size: 3620
SfcI C'TryA_G
Cuts at: 33 663 1348 2341 2532 33
Size: 630 685 993 191 1121
Fragments arranged by size:
1121 993 685 630 191
SfoI GGC'GCC
Cuts at: 1052 1300 1052
Size: 248 3372
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3620
SmaI CCC'GGG
Cuts at: 642 642
Size: 3620
SmlI C'TyrA_G
Cuts at: 775 1690 2182 2444 775
Size: 915 492 262 1951
Fragments arranged by size:
1951 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3620
SphI G_CATG'C
Cuts at: 673 1173 673
Size: 500 3120
SspI AAT'ATT
Cuts at: 203 203
Size: 3620
StyI C'CwwG_G
Cuts at: 218 284 308 332 356 380 404 428 465
Size: 66 24 24 24 24 24 24 37
Cuts at: 465 754 1108 218
Size: 289 354 2730
Fragments arranged by size:
2730 354 289 66 37 24 24 24
24 24 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1203 1336 1795 2004 1203
Size: 133 459 209 2819
Fragments arranged by size:
2819 459 209 133
TatI w'GTAC_w
Cuts at: 3135 3135
Size: 3620
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 249 281 513 545 3137 3169 249
Size: 32 232 32 2592 32 700
Fragments arranged by size:
2592 700 232 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1417 1417
Size: 3620
XbaI T'CTAG_A
Cuts at: 651 651
Size: 3620
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1115 1115
Size: 3620
XmaI C'CCGG_G
Cuts at: 640 640
Size: 3620
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BcgI BfrBI BglI BmgBI BmtI BseRI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I BtgI ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI