Restriction map for
pF1KB8747
(Circular) (Six-base) MAPSORT of: pF1KB8747.seq Check: 3624 from: 1 to: 3206
pF1KB8747 3206 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 244 244
Size: 3206
Acc65I G'GTAC_C
Cuts at: 222 222
Size: 3206
AclI AA'CG_TT
Cuts at: 3046 3046
Size: 3206
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1031 1463 2210 2478 1031
Size: 432 747 268 1759
Fragments arranged by size:
1759 747 432 268
AflIII A'CryG_T
Cuts at: 558 1662 2639 558
Size: 1104 977 1125
Fragments arranged by size:
1125 1104 977
AgeI A'CCGG_T
Cuts at: 1594 1594
Size: 3206
AlwNI CAG_nnn'CTG
Cuts at: 511 2078 511
Size: 1567 1639
ApaLI G'TGCA_C
Cuts at: 1976 1976
Size: 3206
ApoI r'AATT_y
Cuts at: 210 2559 2815 210
Size: 2349 256 601
Fragments arranged by size:
2349 601 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3206
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3206
AvaI C'yCGr_G
Cuts at: 122 226 122
Size: 104 3102
BamHI G'GATC_C
Cuts at: 231 231
Size: 3206
BanI G'GyrC_C
Cuts at: 222 636 884 919 222
Size: 414 248 35 2509
Fragments arranged by size:
2509 414 248 35
BanII G_rGCy'C
Cuts at: 220 1250 220
Size: 1030 2176
BbeI G_GCGC'C
Cuts at: 640 888 640
Size: 248 2958
BciVI GTATCCnnnnn_n'
Cuts at: 1104 1865 3206 1104
Size: 761 1341 1104
Fragments arranged by size:
1341 1104 761
BclI T'GATC_A
Cuts at: 726 726
Size: 3206
BglII A'GATC_T
Cuts at: 721 2758 721
Size: 2037 1169
BlpI GC'TnA_GC
Cuts at: 318 318
Size: 3206
Bme1580I G_kGCm'C
Cuts at: 831 924 1980 831
Size: 93 1056 2057
Fragments arranged by size:
2057 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 418 499 834 2978 418
Size: 81 335 2144 646
Fragments arranged by size:
2144 646 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1573 1573
Size: 3206
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 1261 1753 2051 382
Size: 879 492 298 1537
Fragments arranged by size:
1537 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2910 2910
Size: 3206
BsaAI yAC'GTr
Cuts at: 559 559
Size: 3206
BsaBI GATnn'nnATC
Cuts at: 6 720 2757 6
Size: 714 2037 455
Fragments arranged by size:
2037 714 455
BsaHI Gr'CG_yC
Cuts at: 637 885 1587 3083 637
Size: 248 702 1496 760
Fragments arranged by size:
1496 760 702 248
BsaWI w'CCGG_w
Cuts at: 395 916 1594 1868 2015 2426 395
Size: 521 678 274 147 411 1175
Fragments arranged by size:
1175 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 631 1389 1966 2462 631
Size: 758 577 496 1375
Fragments arranged by size:
1375 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 748 794 2002 104
Size: 644 46 1208 1308
Fragments arranged by size:
1308 1208 644 46
BsiHKAI G_wGCw'C
Cuts at: 220 998 1188 1980 220
Size: 778 190 792 1446
Fragments arranged by size:
1446 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 220 831 924 998 1188 1250 1980 220
Size: 611 93 74 190 62 730 1446
Fragments arranged by size:
1446 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2426 2426
Size: 3206
BspHI T'CATG_A
Cuts at: 2382 2382
Size: 3206
BspMI ACCTGCnnnn'nnnn_
Cuts at: 256 470 772 1153 256
Size: 214 302 381 2309
Fragments arranged by size:
2309 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1498 1552 3036 3201 50
Size: 1448 54 1484 165 55
Fragments arranged by size:
1484 1448 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1118 1118
Size: 3206
BsrFI r'CCGG_y
Cuts at: 1204 1385 1594 1204
Size: 181 209 2816
Fragments arranged by size:
2816 209 181
BssSI C'ACGA_G
Cuts at: 1477 1835 1477
Size: 358 2848
BstAPI GCAn_nnn'nTGC
Cuts at: 583 583
Size: 3206
BstBI TT'CG_AA
Cuts at: 1567 1567
Size: 3206
BstYI r'GATC_y
Cuts at: 1 231 698 721 1056 1302 2314 2400 2412
Size: 230 467 23 335 246 1012 86 12
Cuts at: 2412 2758 1
Size: 346 449
Fragments arranged by size:
1012 467 449 346 335 246 230 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3206
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1156 1337 1500 1156
Size: 181 163 2862
Fragments arranged by size:
2862 181 163
BtsI GCAGTG_nn'
Cuts at: 449 449
Size: 3206
DraI TTT'AAA
Cuts at: 205 205
Size: 3206
DrdI GACnn_nn'nnGTC
Cuts at: 912 1770 912
Size: 858 2348
EaeI y'GGCC_r
Cuts at: 791 965 1356 1383 2728 791
Size: 174 391 27 1345 1269
Fragments arranged by size:
1345 1269 391 174 27
EagI C'GGCC_G
Cuts at: 791 791
Size: 3206
EarI CTCTTCn'nnn_
Cuts at: 1229 1439 1229
Size: 210 2996
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1414 1724 1870 3016 1414
Size: 310 146 1146 1604
Fragments arranged by size:
1604 1146 310 146
EcoICRI GAG'CTC
Cuts at: 218 218
Size: 3206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1031 1463 2210 2478 1031
Size: 432 747 268 1759
Fragments arranged by size:
1759 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 188 345 188
Size: 157 3049
EcoRI G'AATT_C
Cuts at: 210 210
Size: 3206
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1225 1225
Size: 3206
FspI TGC'GCA
Cuts at: 987 987
Size: 3206
HaeII r_GCGC'y
Cuts at: 640 888 1910 2889 640
Size: 248 1022 979 957
Fragments arranged by size:
1022 979 957 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 177 209 1048 1078 1080 1110 177
Size: 32 839 30 2 30 2273
Fragments arranged by size:
2273 839 32 30 30 2
HincII GTy'rAC
Cuts at: 245 245
Size: 3206
KasI G'GCGC_C
Cuts at: 636 884 636
Size: 248 2958
KpnI G_GTAC'C
Cuts at: 226 226
Size: 3206
MluI A'CGCG_T
Cuts at: 2639 2639
Size: 3206
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1877 2061 1877
Size: 184 3022
MscI TGG'CCA
Cuts at: 967 967
Size: 3206
MslI CAynn'nnrTG
Cuts at: 1195 1322 1195
Size: 127 3079
MspA1I CmG'CkG
Cuts at: 317 631 991 2004 2249 317
Size: 314 360 1013 245 1274
Fragments arranged by size:
1274 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1387 1387
Size: 3206
NarI GG'CG_CC
Cuts at: 637 885 637
Size: 248 2958
NcoI C'CATG_G
Cuts at: 107 107
Size: 3206
NgoMIV G'CCGG_C
Cuts at: 1385 1385
Size: 3206
NspI r_CATG'y
Cuts at: 259 759 1666 259
Size: 500 907 1799
Fragments arranged by size:
1799 907 500
PciI A'CATG_T
Cuts at: 1662 1662
Size: 3206
PmeI GTTT'AAAC
Cuts at: 205 205
Size: 3206
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2345 2377 2345
Size: 32 3174
PpuMI rG'GwC_Cy
Cuts at: 188 188
Size: 3206
PstI C_TGCA'G
Cuts at: 253 938 253
Size: 685 2521
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3206
PvuII CAG'CTG
Cuts at: 631 991 631
Size: 360 2846
RsrII CG'GwC_CG
Cuts at: 1401 1401
Size: 3206
SacI G_AGCT'C
Cuts at: 220 220
Size: 3206
SalI G'TCGA_C
Cuts at: 243 243
Size: 3206
SapI GCTCTTCn'nnn_
Cuts at: 1229 1439 1229
Size: 210 2996
SbfI CC_TGCA'GG
Cuts at: 253 253
Size: 3206
ScaI AGT'ACT
Cuts at: 2723 2723
Size: 3206
SfcI C'TryA_G
Cuts at: 33 249 934 1927 2118 33
Size: 216 685 993 191 1121
Fragments arranged by size:
1121 993 685 216 191
SfoI GGC'GCC
Cuts at: 638 886 638
Size: 248 2958
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3206
SmaI CCC'GGG
Cuts at: 124 228 124
Size: 104 3102
SmlI C'TyrA_G
Cuts at: 361 1276 1768 2030 361
Size: 915 492 262 1537
Fragments arranged by size:
1537 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3206
SphI G_CATG'C
Cuts at: 259 759 259
Size: 500 2706
StyI C'CwwG_G
Cuts at: 107 340 694 107
Size: 233 354 2619
Fragments arranged by size:
2619 354 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 789 922 1381 1590 789
Size: 133 459 209 2405
Fragments arranged by size:
2405 459 209 133
TatI w'GTAC_w
Cuts at: 2721 2721
Size: 3206
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2723 2755 2723
Size: 32 3174
Tth111I GACn'n_nGTC
Cuts at: 1003 1003
Size: 3206
XbaI T'CTAG_A
Cuts at: 237 237
Size: 3206
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 701 701
Size: 3206
XmaI C'CCGG_G
Cuts at: 122 226 122
Size: 104 3102
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI