Restriction map for
pF1KB8746
(Circular) (Six-base) MAPSORT of: pF1KB8746.seq Check: 6439 from: 1 to: 3644
pF1KB8746 3644 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 335 335
Size: 3644
AccI GT'mk_AC
Cuts at: 682 682
Size: 3644
Acc65I G'GTAC_C
Cuts at: 660 660
Size: 3644
AclI AA'CG_TT
Cuts at: 3484 3484
Size: 3644
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 1469 1901 2648 2916 280
Size: 1189 432 747 268 1008
Fragments arranged by size:
1189 1008 747 432 268
AflIII A'CryG_T
Cuts at: 996 2100 3077 996
Size: 1104 977 1563
Fragments arranged by size:
1563 1104 977
AgeI A'CCGG_T
Cuts at: 2032 2032
Size: 3644
AlwNI CAG_nnn'CTG
Cuts at: 949 2516 949
Size: 1567 2077
ApaLI G'TGCA_C
Cuts at: 2414 2414
Size: 3644
ApoI r'AATT_y
Cuts at: 648 2997 3253 648
Size: 2349 256 1039
Fragments arranged by size:
2349 1039 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3644
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3644
AvaI C'yCGr_G
Cuts at: 309 421 543 664 309
Size: 112 122 121 3289
Fragments arranged by size:
3289 122 121 112
BamHI G'GATC_C
Cuts at: 669 669
Size: 3644
BanI G'GyrC_C
Cuts at: 660 1074 1322 1357 660
Size: 414 248 35 2947
Fragments arranged by size:
2947 414 248 35
BanII G_rGCy'C
Cuts at: 126 658 1688 126
Size: 532 1030 2082
Fragments arranged by size:
2082 1030 532
BbeI G_GCGC'C
Cuts at: 1078 1326 1078
Size: 248 3396
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3610
BciVI GTATCCnnnnn_n'
Cuts at: 1542 2303 3644 1542
Size: 761 1341 1542
Fragments arranged by size:
1542 1341 761
BclI T'GATC_A
Cuts at: 1164 1164
Size: 3644
BglI GCCn_nnn'nGGC
Cuts at: 256 479 256
Size: 223 3421
BglII A'GATC_T
Cuts at: 1159 3196 1159
Size: 2037 1607
BlpI GC'TnA_GC
Cuts at: 756 756
Size: 3644
Bme1580I G_kGCm'C
Cuts at: 308 1269 1362 2418 308
Size: 961 93 1056 1534
Fragments arranged by size:
1534 1056 961 93
BmgBI CAC'GTC
Cuts at: 561 561
Size: 3644
BmrI ACTGGGnnnn_n'
Cuts at: 856 937 1272 3416 856
Size: 81 335 2144 1084
Fragments arranged by size:
2144 1084 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2011 2011
Size: 3644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 427
Size: 3644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 1699 2191 2489 820
Size: 879 492 298 1975
Fragments arranged by size:
1975 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3348 3348
Size: 3644
BsaAI yAC'GTr
Cuts at: 997 997
Size: 3644
BsaBI GATnn'nnATC
Cuts at: 6 1158 3195 6
Size: 1152 2037 455
Fragments arranged by size:
2037 1152 455
BsaHI Gr'CG_yC
Cuts at: 529 1075 1323 2025 3521 529
Size: 546 248 702 1496 652
Fragments arranged by size:
1496 702 652 546 248
BsaWI w'CCGG_w
Cuts at: 833 1354 2032 2306 2453 2864 833
Size: 521 678 274 147 411 1613
Fragments arranged by size:
1613 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 169 332 454 477 501 585 1069 1827 2404
Size: 163 122 23 24 84 484 758 577
Cuts at: 2404 2900 169
Size: 496 913
Fragments arranged by size:
913 758 577 496 484 163 122 84
24 23
BsiEI CG_ry'CG
Cuts at: 104 1186 1232 2440 104
Size: 1082 46 1208 1308
Fragments arranged by size:
1308 1208 1082 46
BsiHKAI G_wGCw'C
Cuts at: 658 1436 1626 2418 658
Size: 778 190 792 1884
Fragments arranged by size:
1884 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 126 308 658 1269 1362 1436 1626 1688 2418
Size: 182 350 611 93 74 190 62 730
Cuts at: 2418 126
Size: 1352
Fragments arranged by size:
1352 730 611 350 190 182 93 74
62
BspEI T'CCGG_A
Cuts at: 2864 2864
Size: 3644
BspHI T'CATG_A
Cuts at: 2820 2820
Size: 3644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 190 335 426 694 908 1210 1591 190
Size: 145 91 268 214 302 381 2243
Fragments arranged by size:
2243 381 302 268 214 145 91
BsrBI CCG'CTC
Cuts at: 50 1936 1990 3474 3639 50
Size: 1886 54 1484 165 55
Fragments arranged by size:
1886 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1556 1556
Size: 3644
BsrFI r'CCGG_y
Cuts at: 255 482 1642 1823 2032 255
Size: 227 1160 181 209 1867
Fragments arranged by size:
1867 1160 227 209 181
BssSI C'ACGA_G
Cuts at: 1915 2273 1915
Size: 358 3286
BstAPI GCAn_nnn'nTGC
Cuts at: 1021 1021
Size: 3644
BstBI TT'CG_AA
Cuts at: 2005 2005
Size: 3644
BstYI r'GATC_y
Cuts at: 1 669 1136 1159 1494 1740 2752 2838 2850
Size: 668 467 23 335 246 1012 86 12
Cuts at: 2850 3196 1
Size: 346 449
Fragments arranged by size:
1012 668 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 200 228 468 575 200
Size: 28 240 107 3269
Fragments arranged by size:
3269 240 107 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1594 1775 1938 1594
Size: 181 163 3300
Fragments arranged by size:
3300 181 163
BtsI GCAGTG_nn'
Cuts at: 887 887
Size: 3644
DraI TTT'AAA
Cuts at: 643 643
Size: 3644
DrdI GACnn_nn'nnGTC
Cuts at: 1350 2208 1350
Size: 858 2786
EaeI y'GGCC_r
Cuts at: 281 578 1229 1403 1794 1821 3166 281
Size: 297 651 174 391 27 1345 759
Fragments arranged by size:
1345 759 651 391 297 174 27
EagI C'GGCC_G
Cuts at: 1229 1229
Size: 3644
EarI CTCTTCn'nnn_
Cuts at: 602 1667 1877 602
Size: 1065 210 2369
Fragments arranged by size:
2369 1065 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1852 2162 2308 3454 1852
Size: 310 146 1146 2042
Fragments arranged by size:
2042 1146 310 146
EcoICRI GAG'CTC
Cuts at: 656 656
Size: 3644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 427 1469 1901 2648 2916 280
Size: 147 1042 432 747 268 1008
Fragments arranged by size:
1042 1008 747 432 268 147
EcoNI CCTnn'n_nnAGG
Cuts at: 433 433
Size: 3644
EcoO109I rG'GnC_Cy
Cuts at: 426 630 783 426
Size: 204 153 3287
Fragments arranged by size:
3287 204 153
EcoRI G'AATT_C
Cuts at: 648 648
Size: 3644
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1663 1663
Size: 3644
FspI TGC'GCA
Cuts at: 1425 1425
Size: 3644
HaeII r_GCGC'y
Cuts at: 568 1078 1326 2348 3327 568
Size: 510 248 1022 979 885
Fragments arranged by size:
1022 979 885 510 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1486 1516 1518 1548 1486
Size: 30 2 30 3582
Fragments arranged by size:
3582 30 30 2
HincII GTy'rAC
Cuts at: 683 683
Size: 3644
KasI G'GCGC_C
Cuts at: 1074 1322 1074
Size: 248 3396
KpnI G_GTAC'C
Cuts at: 664 664
Size: 3644
MluI A'CGCG_T
Cuts at: 3077 3077
Size: 3644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2315 2499 2315
Size: 184 3460
MscI TGG'CCA
Cuts at: 283 1405 283
Size: 1122 2522
MslI CAynn'nnrTG
Cuts at: 1633 1760 1633
Size: 127 3517
MspA1I CmG'CkG
Cuts at: 336 443 477 501 509 522 755 1069 1429
Size: 107 34 24 8 13 233 314 360
Cuts at: 1429 2442 2687 336
Size: 1013 245 1293
Fragments arranged by size:
1293 1013 360 314 245 233 107 34
24 13 8
NaeI GCC'GGC
Cuts at: 257 484 1825 257
Size: 227 1341 2076
Fragments arranged by size:
2076 1341 227
NarI GG'CG_CC
Cuts at: 1075 1323 1075
Size: 248 3396
NcoI C'CATG_G
Cuts at: 200 468 200
Size: 268 3376
NgoMIV G'CCGG_C
Cuts at: 255 482 1823 255
Size: 227 1341 2076
Fragments arranged by size:
2076 1341 227
NspI r_CATG'y
Cuts at: 697 1197 2104 697
Size: 500 907 2237
Fragments arranged by size:
2237 907 500
PciI A'CATG_T
Cuts at: 2100 2100
Size: 3644
PmeI GTTT'AAAC
Cuts at: 643 643
Size: 3644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2783 2815 2783
Size: 32 3612
PstI C_TGCA'G
Cuts at: 223 341 382 437 527 691 1376 223
Size: 118 41 55 90 164 685 2491
Fragments arranged by size:
2491 685 164 118 90 55 41
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3644
PvuII CAG'CTG
Cuts at: 336 501 509 522 1069 1429 336
Size: 165 8 13 547 360 2551
Fragments arranged by size:
2551 547 360 165 13 8
RsrII CG'GwC_CG
Cuts at: 1839 1839
Size: 3644
SacI G_AGCT'C
Cuts at: 658 658
Size: 3644
SalI G'TCGA_C
Cuts at: 681 681
Size: 3644
SapI GCTCTTCn'nnn_
Cuts at: 602 1667 1877 602
Size: 1065 210 2369
Fragments arranged by size:
2369 1065 210
SbfI CC_TGCA'GG
Cuts at: 691 691
Size: 3644
ScaI AGT'ACT
Cuts at: 3161 3161
Size: 3644
SfcI C'TryA_G
Cuts at: 33 219 337 378 433 523 687 1372 2365
Size: 186 118 41 55 90 164 685 993
Cuts at: 2365 2556 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 191 186 164 118 90
55 41
SfiI GGCCn_nnn'nGGCC
Cuts at: 479 479
Size: 3644
SfoI GGC'GCC
Cuts at: 1076 1324 1076
Size: 248 3396
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3644
SmaI CCC'GGG
Cuts at: 311 545 666 311
Size: 234 121 3289
Fragments arranged by size:
3289 234 121
SmlI C'TyrA_G
Cuts at: 799 1714 2206 2468 799
Size: 915 492 262 1975
Fragments arranged by size:
1975 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3644
SphI G_CATG'C
Cuts at: 697 1197 697
Size: 500 3144
StyI C'CwwG_G
Cuts at: 158 200 468 778 1132 158
Size: 42 268 310 354 2670
Fragments arranged by size:
2670 354 310 268 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 201 525 1227 1360 1819 2028 201
Size: 324 702 133 459 209 1817
Fragments arranged by size:
1817 702 459 324 209 133
TatI w'GTAC_w
Cuts at: 3159 3159
Size: 3644
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3161 3193 3161
Size: 32 3612
Tth111I GACn'n_nGTC
Cuts at: 1441 1441
Size: 3644
XbaI T'CTAG_A
Cuts at: 675 675
Size: 3644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 630 1139 630
Size: 509 3135
XmaI C'CCGG_G
Cuts at: 309 543 664 309
Size: 234 121 3289
Fragments arranged by size:
3289 234 121
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I
BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI