Restriction map for
pF1KB8745
(Circular) (Six-base) MAPSORT of: pF1KB8745.seq Check: 8462 from: 1 to: 4319
pF1KB8745 4319 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1357 1357
Size: 4319
Acc65I G'GTAC_C
Cuts at: 1335 1335
Size: 4319
AclI AA'CG_TT
Cuts at: 4159 4159
Size: 4319
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 2144 2576 3323 3591 394
Size: 1750 432 747 268 1122
Fragments arranged by size:
1750 1122 747 432 268
AfeI AGC'GCT
Cuts at: 361 361
Size: 4319
AflII C'TTAA_G
Cuts at: 682 682
Size: 4319
AflIII A'CryG_T
Cuts at: 126 1671 2775 3752 126
Size: 1545 1104 977 693
Fragments arranged by size:
1545 1104 977 693
AgeI A'CCGG_T
Cuts at: 2707 2707
Size: 4319
AlwNI CAG_nnn'CTG
Cuts at: 1245 1624 3191 1245
Size: 379 1567 2373
Fragments arranged by size:
2373 1567 379
ApaI G_GGCC'C
Cuts at: 174 385 174
Size: 211 4108
ApaLI G'TGCA_C
Cuts at: 3089 3089
Size: 4319
ApoI r'AATT_y
Cuts at: 505 1221 1323 3672 3928 505
Size: 716 102 2349 256 896
Fragments arranged by size:
2349 896 716 256 102
AseI AT'TA_AT
Cuts at: 20 20
Size: 4319
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4319
AvaI C'yCGr_G
Cuts at: 252 1339 252
Size: 1087 3232
BamHI G'GATC_C
Cuts at: 1344 1344
Size: 4319
BanI G'GyrC_C
Cuts at: 264 979 1335 1749 1997 2032 264
Size: 715 356 414 248 35 2551
Fragments arranged by size:
2551 715 414 356 248 35
BanII G_rGCy'C
Cuts at: 174 385 859 1056 1333 2363 174
Size: 211 474 197 277 1030 2130
Fragments arranged by size:
2130 1030 474 277 211 197
BbeI G_GCGC'C
Cuts at: 1753 2001 1753
Size: 248 4071
BbvCI CC'TCA_GC
Cuts at: 184 184
Size: 4319
BciVI GTATCCnnnnn_n'
Cuts at: 2217 2978 4319 2217
Size: 761 1341 2217
Fragments arranged by size:
2217 1341 761
BclI T'GATC_A
Cuts at: 1226 1839 1226
Size: 613 3706
BglI GCCn_nnn'nGGC
Cuts at: 226 226
Size: 4319
BglII A'GATC_T
Cuts at: 1834 3871 1834
Size: 2037 2282
BlpI GC'TnA_GC
Cuts at: 1431 1431
Size: 4319
Bme1580I G_kGCm'C
Cuts at: 174 385 984 1944 2037 3093 174
Size: 211 599 960 93 1056 1400
Fragments arranged by size:
1400 1056 960 599 211 93
BmrI ACTGGGnnnn_n'
Cuts at: 1531 1612 1947 4091 1531
Size: 81 335 2144 1759
Fragments arranged by size:
2144 1759 335 81
BmtI G_CTAG'C
Cuts at: 116 116
Size: 4319
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 558 2686 558
Size: 2128 2191
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 757 1222 559
Size: 198 465 3656
Fragments arranged by size:
3656 465 198
Bpu10I CC'TnA_GC
Cuts at: 184 184
Size: 4319
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 512 1495 2374 2866 3164 340
Size: 172 983 879 492 298 1495
Fragments arranged by size:
1495 983 879 492 298 172
BsaI GGTCTCn'nnnn_
Cuts at: 4023 4023
Size: 4319
BsaAI yAC'GTr
Cuts at: 127 1672 127
Size: 1545 2774
BsaBI GATnn'nnATC
Cuts at: 6 871 1833 3870 6
Size: 865 962 2037 455
Fragments arranged by size:
2037 962 865 455
BsaHI Gr'CG_yC
Cuts at: 317 1750 1998 2700 4196 317
Size: 1433 248 702 1496 440
Fragments arranged by size:
1496 1433 702 440 248
BsaWI w'CCGG_w
Cuts at: 620 1508 2029 2707 2981 3128 3539 620
Size: 888 521 678 274 147 411 1400
Fragments arranged by size:
1400 888 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 358 358
Size: 4319
BseYI C'CCAG_C
Cuts at: 1744 2502 3079 3575 1744
Size: 758 577 496 2488
Fragments arranged by size:
2488 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 589
Size: 4319
BsiEI CG_ry'CG
Cuts at: 104 1861 1907 3115 104
Size: 1757 46 1208 1308
Fragments arranged by size:
1757 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1333 2111 2301 3093 1333
Size: 778 190 792 2559
Fragments arranged by size:
2559 792 778 190
BsmI GAATG_Cn'
Cuts at: 156 156
Size: 4319
Bsp1286I G_dGCh'C
Cuts at: 174 385 859 984 1056 1333 1944 2037 2111
Size: 211 474 125 72 277 611 93 74
Cuts at: 2111 2301 2363 3093 174
Size: 190 62 730 1400
Fragments arranged by size:
1400 730 611 474 277 211 190 125
93 74 72 62
BspEI T'CCGG_A
Cuts at: 3539 3539
Size: 4319
BspHI T'CATG_A
Cuts at: 3495 3495
Size: 4319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1369 1583 1885 2266 1369
Size: 214 302 381 3422
Fragments arranged by size:
3422 381 302 214
BsrBI CCG'CTC
Cuts at: 50 720 2611 2665 4149 4314 50
Size: 670 1891 54 1484 165 55
Fragments arranged by size:
1891 1484 670 165 55 54
BsrDI GCAATG_nn'
Cuts at: 119 897 2231 119
Size: 778 1334 2207
Fragments arranged by size:
2207 1334 778
BsrFI r'CCGG_y
Cuts at: 140 2317 2498 2707 140
Size: 2177 181 209 1752
Fragments arranged by size:
2177 1752 209 181
BssSI C'ACGA_G
Cuts at: 2590 2948 2590
Size: 358 3961
BstAPI GCAn_nnn'nTGC
Cuts at: 1696 1696
Size: 4319
BstBI TT'CG_AA
Cuts at: 2680 2680
Size: 4319
BstXI CCAn_nnnn'nTGG
Cuts at: 313 462 735 313
Size: 149 273 3897
Fragments arranged by size:
3897 273 149
BstYI r'GATC_y
Cuts at: 1 1344 1811 1834 2169 2415 3427 3513 3525
Size: 1343 467 23 335 246 1012 86 12
Cuts at: 3525 3871 1
Size: 346 449
Fragments arranged by size:
1343 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 376 1010 376
Size: 634 3685
BtgI C'CryG_G
Cuts at: 107 498 107
Size: 391 3928
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2269 2450 2613 2269
Size: 181 163 3975
Fragments arranged by size:
3975 181 163
BtsI GCAGTG_nn'
Cuts at: 492 885 1562 492
Size: 393 677 3249
Fragments arranged by size:
3249 677 393
DraI TTT'AAA
Cuts at: 1318 1318
Size: 4319
DrdI GACnn_nn'nnGTC
Cuts at: 2025 2883 2025
Size: 858 3461
EaeI y'GGCC_r
Cuts at: 218 1904 2078 2469 2496 3841 218
Size: 1686 174 391 27 1345 696
Fragments arranged by size:
1686 1345 696 391 174 27
EagI C'GGCC_G
Cuts at: 1904 1904
Size: 4319
EarI CTCTTCn'nnn_
Cuts at: 758 2342 2552 758
Size: 1584 210 2525
Fragments arranged by size:
2525 1584 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2527 2837 2983 4129 2527
Size: 310 146 1146 2717
Fragments arranged by size:
2717 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1331 1331
Size: 4319
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 559 757 1222 2144 2576 3323 3591 394
Size: 165 198 465 922 432 747 268 1122
Fragments arranged by size:
1122 922 747 465 432 268 198 165
EcoO109I rG'GnC_Cy
Cuts at: 188 381 382 666 957 1458 188
Size: 193 1 284 291 501 3049
Fragments arranged by size:
3049 501 291 284 193 1
EcoRI G'AATT_C
Cuts at: 1323 1323
Size: 4319
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 562 2338 562
Size: 1776 2543
FspI TGC'GCA
Cuts at: 2100 2100
Size: 4319
HaeII r_GCGC'y
Cuts at: 363 1753 2001 3023 4002 363
Size: 1390 248 1022 979 680
Fragments arranged by size:
1390 1022 979 680 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1218 1250 2161 2191 2193 2223 1218
Size: 32 911 30 2 30 3314
Fragments arranged by size:
3314 911 32 30 30 2
HincII GTy'rAC
Cuts at: 1358 1358
Size: 4319
KasI G'GCGC_C
Cuts at: 1749 1997 1749
Size: 248 4071
KpnI G_GTAC'C
Cuts at: 1339 1339
Size: 4319
MluI A'CGCG_T
Cuts at: 3752 3752
Size: 4319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 696 2990 3174 696
Size: 2294 184 1841
Fragments arranged by size:
2294 1841 184
MscI TGG'CCA
Cuts at: 220 2080 220
Size: 1860 2459
MslI CAynn'nnrTG
Cuts at: 403 2308 2435 403
Size: 1905 127 2287
Fragments arranged by size:
2287 1905 127
MspA1I CmG'CkG
Cuts at: 1145 1430 1744 2104 3117 3362 1145
Size: 285 314 360 1013 245 2102
Fragments arranged by size:
2102 1013 360 314 285 245
NaeI GCC'GGC
Cuts at: 142 2500 142
Size: 2358 1961
NarI GG'CG_CC
Cuts at: 1750 1998 1750
Size: 248 4071
NcoI C'CATG_G
Cuts at: 107 498 107
Size: 391 3928
NgoMIV G'CCGG_C
Cuts at: 140 2498 140
Size: 2358 1961
NheI G'CTAG_C
Cuts at: 112 112
Size: 4319
NspI r_CATG'y
Cuts at: 1372 1872 2779 1372
Size: 500 907 2912
Fragments arranged by size:
2912 907 500
PasI CC'CwG_GG
Cuts at: 192 192
Size: 4319
PciI A'CATG_T
Cuts at: 2775 2775
Size: 4319
PfoI T'CCnGG_A
Cuts at: 1238 1238
Size: 4319
PmeI GTTT'AAAC
Cuts at: 1318 1318
Size: 4319
PmlI CAC'GTG
Cuts at: 127 127
Size: 4319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3458 3490 3458
Size: 32 4287
PpuMI rG'GwC_Cy
Cuts at: 188 666 957 188
Size: 478 291 3550
Fragments arranged by size:
3550 478 291
PshAI GACnn'nnGTC
Cuts at: 307 307
Size: 4319
PspOMI G'GGCC_C
Cuts at: 170 381 170
Size: 211 4108
PstI C_TGCA'G
Cuts at: 1366 2051 1366
Size: 685 3634
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4319
PvuII CAG'CTG
Cuts at: 1744 2104 1744
Size: 360 3959
RsrII CG'GwC_CG
Cuts at: 2514 2514
Size: 4319
SacI G_AGCT'C
Cuts at: 1333 1333
Size: 4319
SalI G'TCGA_C
Cuts at: 1356 1356
Size: 4319
SapI GCTCTTCn'nnn_
Cuts at: 2342 2552 2342
Size: 210 4109
SbfI CC_TGCA'GG
Cuts at: 1366 1366
Size: 4319
ScaI AGT'ACT
Cuts at: 3836 3836
Size: 4319
SfcI C'TryA_G
Cuts at: 33 1362 2047 3040 3231 33
Size: 1329 685 993 191 1121
Fragments arranged by size:
1329 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1751 1999 1751
Size: 248 4071
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4319
SmaI CCC'GGG
Cuts at: 1341 1341
Size: 4319
SmlI C'TyrA_G
Cuts at: 355 527 682 1474 2389 2881 3143 355
Size: 172 155 792 915 492 262 1531
Fragments arranged by size:
1531 915 792 492 262 172 155
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4319
SphI G_CATG'C
Cuts at: 1372 1872 1372
Size: 500 3819
StyI C'CwwG_G
Cuts at: 107 166 273 498 1453 1807 107
Size: 59 107 225 955 354 2619
Fragments arranged by size:
2619 955 354 225 107 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 196 1902 2035 2494 2703 196
Size: 1706 133 459 209 1812
Fragments arranged by size:
1812 1706 459 209 133
TatI w'GTAC_w
Cuts at: 3834 3834
Size: 4319
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3836 3868 3836
Size: 32 4287
Tth111I GACn'n_nGTC
Cuts at: 2116 2116
Size: 4319
XbaI T'CTAG_A
Cuts at: 1350 1350
Size: 4319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 971 1814 971
Size: 843 3476
XmaI C'CCGG_G
Cuts at: 1339 1339
Size: 4319
XmnI GAAnn'nnTTC
Cuts at: 247 247
Size: 4319
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmgBI BsaXI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI
NruI NsiI PacI PflMI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI