Restriction map for
pF1KB8744
(Circular) (Six-base) MAPSORT of: pF1KB8744.seq Check: 5581 from: 1 to: 3494
pF1KB8744 3494 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 306 306
Size: 3494
AccI GT'mk_AC
Cuts at: 532 532
Size: 3494
Acc65I G'GTAC_C
Cuts at: 510 510
Size: 3494
AclI AA'CG_TT
Cuts at: 3334 3334
Size: 3494
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1319 1751 2498 2766 1319
Size: 432 747 268 2047
Fragments arranged by size:
2047 747 432 268
AflIII A'CryG_T
Cuts at: 846 1950 2927 846
Size: 1104 977 1413
Fragments arranged by size:
1413 1104 977
AgeI A'CCGG_T
Cuts at: 1882 1882
Size: 3494
AhdI GACnn_n'nnGTC
Cuts at: 225 225
Size: 3494
AleI CACnn'nnGTG
Cuts at: 362 362
Size: 3494
AlwNI CAG_nnn'CTG
Cuts at: 189 799 2366 189
Size: 610 1567 1317
Fragments arranged by size:
1567 1317 610
ApaLI G'TGCA_C
Cuts at: 2264 2264
Size: 3494
ApoI r'AATT_y
Cuts at: 498 2847 3103 498
Size: 2349 256 889
Fragments arranged by size:
2349 889 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3494
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3494
AvaI C'yCGr_G
Cuts at: 166 360 514 166
Size: 194 154 3146
Fragments arranged by size:
3146 194 154
BamHI G'GATC_C
Cuts at: 519 519
Size: 3494
BanI G'GyrC_C
Cuts at: 510 924 1172 1207 510
Size: 414 248 35 2797
Fragments arranged by size:
2797 414 248 35
BanII G_rGCy'C
Cuts at: 167 458 508 1538 167
Size: 291 50 1030 2123
Fragments arranged by size:
2123 1030 291 50
BbeI G_GCGC'C
Cuts at: 928 1176 928
Size: 248 3246
BbsI GAAGACnn'nnnn_
Cuts at: 476 476
Size: 3494
BbvCI CC'TCA_GC
Cuts at: 407 407
Size: 3494
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3460
BciVI GTATCCnnnnn_n'
Cuts at: 1392 2153 3494 1392
Size: 761 1341 1392
Fragments arranged by size:
1392 1341 761
BclI T'GATC_A
Cuts at: 1014 1014
Size: 3494
BglII A'GATC_T
Cuts at: 1009 3046 1009
Size: 2037 1457
BlpI GC'TnA_GC
Cuts at: 606 606
Size: 3494
Bme1580I G_kGCm'C
Cuts at: 1119 1212 2268 1119
Size: 93 1056 2345
Fragments arranged by size:
2345 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 706 787 1122 3266 706
Size: 81 335 2144 934
Fragments arranged by size:
2144 934 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1861 1861
Size: 3494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 181
Size: 3494
Bpu10I CC'TnA_GC
Cuts at: 407 407
Size: 3494
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 1549 2041 2339 670
Size: 879 492 298 1825
Fragments arranged by size:
1825 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3198 3198
Size: 3494
BsaAI yAC'GTr
Cuts at: 351 847 351
Size: 496 2998
BsaBI GATnn'nnATC
Cuts at: 6 1008 3045 6
Size: 1002 2037 455
Fragments arranged by size:
2037 1002 455
BsaHI Gr'CG_yC
Cuts at: 925 1173 1875 3371 925
Size: 248 702 1496 1048
Fragments arranged by size:
1496 1048 702 248
BsaWI w'CCGG_w
Cuts at: 683 1204 1882 2156 2303 2714 683
Size: 521 678 274 147 411 1463
Fragments arranged by size:
1463 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 346 424 184
Size: 162 78 3254
Fragments arranged by size:
3254 162 78
BseYI C'CCAG_C
Cuts at: 919 1677 2254 2750 919
Size: 758 577 496 1663
Fragments arranged by size:
1663 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 373
Size: 3494
BsiEI CG_ry'CG
Cuts at: 104 1036 1082 2290 104
Size: 932 46 1208 1308
Fragments arranged by size:
1308 1208 932 46
BsiHKAI G_wGCw'C
Cuts at: 245 458 508 1286 1476 2268 245
Size: 213 50 778 190 792 1471
Fragments arranged by size:
1471 792 778 213 190 50
BsmBI CGTCTCn'nnnn_
Cuts at: 233 233
Size: 3494
Bsp1286I G_dGCh'C
Cuts at: 167 245 458 508 1119 1212 1286 1476 1538
Size: 78 213 50 611 93 74 190 62
Cuts at: 1538 2268 167
Size: 730 1393
Fragments arranged by size:
1393 730 611 213 190 93 78 74
62 50
BspEI T'CCGG_A
Cuts at: 2714 2714
Size: 3494
BspHI T'CATG_A
Cuts at: 2670 2670
Size: 3494
BspMI ACCTGCnnnn'nnnn_
Cuts at: 266 306 544 758 1060 1441 266
Size: 40 238 214 302 381 2319
Fragments arranged by size:
2319 381 302 238 214 40
BsrBI CCG'CTC
Cuts at: 50 1786 1840 3324 3489 50
Size: 1736 54 1484 165 55
Fragments arranged by size:
1736 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1406 1406
Size: 3494
BsrFI r'CCGG_y
Cuts at: 1492 1673 1882 1492
Size: 181 209 3104
Fragments arranged by size:
3104 209 181
BssSI C'ACGA_G
Cuts at: 1765 2123 1765
Size: 358 3136
BstAPI GCAn_nnn'nTGC
Cuts at: 189 871 189
Size: 682 2812
BstBI TT'CG_AA
Cuts at: 1855 1855
Size: 3494
BstYI r'GATC_y
Cuts at: 1 519 986 1009 1344 1590 2602 2688 2700
Size: 518 467 23 335 246 1012 86 12
Cuts at: 2700 3046 1
Size: 346 449
Fragments arranged by size:
1012 518 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 143 143
Size: 3494
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 207 1444 1625 1788 207
Size: 1237 181 163 1913
Fragments arranged by size:
1913 1237 181 163
BtsI GCAGTG_nn'
Cuts at: 737 737
Size: 3494
DraI TTT'AAA
Cuts at: 493 493
Size: 3494
DrdI GACnn_nn'nnGTC
Cuts at: 1200 2058 1200
Size: 858 2636
EaeI y'GGCC_r
Cuts at: 1079 1253 1644 1671 3016 1079
Size: 174 391 27 1345 1557
Fragments arranged by size:
1557 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1079 1079
Size: 3494
EarI CTCTTCn'nnn_
Cuts at: 1517 1727 1517
Size: 210 3284
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1702 2012 2158 3304 1702
Size: 310 146 1146 1892
Fragments arranged by size:
1892 1146 310 146
EcoICRI GAG'CTC
Cuts at: 456 506 456
Size: 50 3444
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 1319 1751 2498 2766 181
Size: 1138 432 747 268 909
Fragments arranged by size:
1138 909 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 633 633
Size: 3494
EcoRI G'AATT_C
Cuts at: 498 498
Size: 3494
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 500 1513 500
Size: 1013 2481
FspI TGC'GCA
Cuts at: 1275 1275
Size: 3494
HaeII r_GCGC'y
Cuts at: 142 341 928 1176 2198 3177 142
Size: 199 587 248 1022 979 459
Fragments arranged by size:
1022 979 587 459 248 199
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 211 243 370 402 1336 1366 1368 1398 211
Size: 32 127 32 934 30 2 30 2307
Fragments arranged by size:
2307 934 127 32 32 30 30 2
HincII GTy'rAC
Cuts at: 533 533
Size: 3494
KasI G'GCGC_C
Cuts at: 924 1172 924
Size: 248 3246
KpnI G_GTAC'C
Cuts at: 514 514
Size: 3494
MluI A'CGCG_T
Cuts at: 2927 2927
Size: 3494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 368 2165 2349 368
Size: 1797 184 1513
Fragments arranged by size:
1797 1513 184
MscI TGG'CCA
Cuts at: 1255 1255
Size: 3494
MslI CAynn'nnrTG
Cuts at: 362 1483 1610 362
Size: 1121 127 2246
Fragments arranged by size:
2246 1121 127
MspA1I CmG'CkG
Cuts at: 605 919 1279 2292 2537 605
Size: 314 360 1013 245 1562
Fragments arranged by size:
1562 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1675 1675
Size: 3494
NarI GG'CG_CC
Cuts at: 925 1173 925
Size: 248 3246
NgoMIV G'CCGG_C
Cuts at: 1673 1673
Size: 3494
NspI r_CATG'y
Cuts at: 547 1047 1954 547
Size: 500 907 2087
Fragments arranged by size:
2087 907 500
PciI A'CATG_T
Cuts at: 1950 1950
Size: 3494
PmeI GTTT'AAAC
Cuts at: 493 493
Size: 3494
PmlI CAC'GTG
Cuts at: 351 351
Size: 3494
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2633 2665 2633
Size: 32 3462
PstI C_TGCA'G
Cuts at: 541 1226 541
Size: 685 2809
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3494
PvuII CAG'CTG
Cuts at: 919 1279 919
Size: 360 3134
RsrII CG'GwC_CG
Cuts at: 1689 1689
Size: 3494
SacI G_AGCT'C
Cuts at: 458 508 458
Size: 50 3444
SalI G'TCGA_C
Cuts at: 531 531
Size: 3494
SapI GCTCTTCn'nnn_
Cuts at: 1517 1727 1517
Size: 210 3284
SbfI CC_TGCA'GG
Cuts at: 541 541
Size: 3494
ScaI AGT'ACT
Cuts at: 3011 3011
Size: 3494
SfcI C'TryA_G
Cuts at: 33 537 1222 2215 2406 33
Size: 504 685 993 191 1121
Fragments arranged by size:
1121 993 685 504 191
SfoI GGC'GCC
Cuts at: 926 1174 926
Size: 248 3246
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3494
SmaI CCC'GGG
Cuts at: 516 516
Size: 3494
SmlI C'TyrA_G
Cuts at: 649 1564 2056 2318 649
Size: 915 492 262 1825
Fragments arranged by size:
1825 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3494
SphI G_CATG'C
Cuts at: 547 1047 547
Size: 500 2994
StuI AGG'CCT
Cuts at: 325 325
Size: 3494
StyI C'CwwG_G
Cuts at: 419 628 982 419
Size: 209 354 2931
Fragments arranged by size:
2931 354 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1077 1210 1669 1878 1077
Size: 133 459 209 2693
Fragments arranged by size:
2693 459 209 133
TatI w'GTAC_w
Cuts at: 3009 3009
Size: 3494
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3011 3043 3011
Size: 32 3462
Tth111I GACn'n_nGTC
Cuts at: 1291 1291
Size: 3494
XbaI T'CTAG_A
Cuts at: 525 525
Size: 3494
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 989 114
Size: 875 2619
XmaI C'CCGG_G
Cuts at: 514 514
Size: 3494
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PmlI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI ApaI AscI AvrII
BaeI BfrBI BglI BmgBI BmtI BsaXI BsiWI BsmI
BsrGI BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI