Restriction map for
pF1KB8739
(Circular) (Six-base) MAPSORT of: pF1KB8739.seq Check: 1099 from: 1 to: 3269
pF1KB8739 3269 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 307 307
Size: 3269
Acc65I G'GTAC_C
Cuts at: 285 285
Size: 3269
AclI AA'CG_TT
Cuts at: 3109 3109
Size: 3269
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1094 1526 2273 2541 1094
Size: 432 747 268 1822
Fragments arranged by size:
1822 747 432 268
AflIII A'CryG_T
Cuts at: 621 1725 2702 621
Size: 1104 977 1188
Fragments arranged by size:
1188 1104 977
AgeI A'CCGG_T
Cuts at: 1657 1657
Size: 3269
AlwNI CAG_nnn'CTG
Cuts at: 574 2141 574
Size: 1567 1702
ApaLI G'TGCA_C
Cuts at: 2039 2039
Size: 3269
ApoI r'AATT_y
Cuts at: 273 2622 2878 273
Size: 2349 256 664
Fragments arranged by size:
2349 664 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3269
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3269
AvaI C'yCGr_G
Cuts at: 289 289
Size: 3269
BamHI G'GATC_C
Cuts at: 294 294
Size: 3269
BanI G'GyrC_C
Cuts at: 285 699 947 982 285
Size: 414 248 35 2572
Fragments arranged by size:
2572 414 248 35
BanII G_rGCy'C
Cuts at: 283 1313 283
Size: 1030 2239
BbeI G_GCGC'C
Cuts at: 703 951 703
Size: 248 3021
BciVI GTATCCnnnnn_n'
Cuts at: 1167 1928 3269 1167
Size: 761 1341 1167
Fragments arranged by size:
1341 1167 761
BclI T'GATC_A
Cuts at: 789 789
Size: 3269
BglII A'GATC_T
Cuts at: 784 2821 784
Size: 2037 1232
BlpI GC'TnA_GC
Cuts at: 135 381 135
Size: 246 3023
Bme1580I G_kGCm'C
Cuts at: 894 987 2043 894
Size: 93 1056 2120
Fragments arranged by size:
2120 1056 93
BmgBI CAC'GTC
Cuts at: 181 181
Size: 3269
BmrI ACTGGGnnnn_n'
Cuts at: 481 562 897 3041 481
Size: 81 335 2144 709
Fragments arranged by size:
2144 709 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1636 1636
Size: 3269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 1324 1816 2114 445
Size: 879 492 298 1600
Fragments arranged by size:
1600 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2973 2973
Size: 3269
BsaAI yAC'GTr
Cuts at: 622 622
Size: 3269
BsaBI GATnn'nnATC
Cuts at: 6 244 783 2820 6
Size: 238 539 2037 455
Fragments arranged by size:
2037 539 455 238
BsaHI Gr'CG_yC
Cuts at: 700 948 1650 3146 700
Size: 248 702 1496 823
Fragments arranged by size:
1496 823 702 248
BsaWI w'CCGG_w
Cuts at: 458 979 1657 1931 2078 2489 458
Size: 521 678 274 147 411 1238
Fragments arranged by size:
1238 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 694 1452 2029 2525 694
Size: 758 577 496 1438
Fragments arranged by size:
1438 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 811 857 2065 104
Size: 707 46 1208 1308
Fragments arranged by size:
1308 1208 707 46
BsiHKAI G_wGCw'C
Cuts at: 283 1061 1251 2043 283
Size: 778 190 792 1509
Fragments arranged by size:
1509 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 283 894 987 1061 1251 1313 2043 283
Size: 611 93 74 190 62 730 1509
Fragments arranged by size:
1509 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2489 2489
Size: 3269
BspHI T'CATG_A
Cuts at: 2445 2445
Size: 3269
BspMI ACCTGCnnnn'nnnn_
Cuts at: 319 533 835 1216 319
Size: 214 302 381 2372
Fragments arranged by size:
2372 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1561 1615 3099 3264 50
Size: 1511 54 1484 165 55
Fragments arranged by size:
1511 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1181 1181
Size: 3269
BsrFI r'CCGG_y
Cuts at: 1267 1448 1657 1267
Size: 181 209 2879
Fragments arranged by size:
2879 209 181
BssSI C'ACGA_G
Cuts at: 1540 1898 1540
Size: 358 2911
BstAPI GCAn_nnn'nTGC
Cuts at: 646 646
Size: 3269
BstBI TT'CG_AA
Cuts at: 1630 1630
Size: 3269
BstYI r'GATC_y
Cuts at: 1 294 761 784 1119 1365 2377 2463 2475
Size: 293 467 23 335 246 1012 86 12
Cuts at: 2475 2821 1
Size: 346 449
Fragments arranged by size:
1012 467 449 346 335 293 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3269
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1219 1400 1563 1219
Size: 181 163 2925
Fragments arranged by size:
2925 181 163
BtsI GCAGTG_nn'
Cuts at: 512 512
Size: 3269
DraI TTT'AAA
Cuts at: 268 268
Size: 3269
DrdI GACnn_nn'nnGTC
Cuts at: 975 1833 975
Size: 858 2411
EaeI y'GGCC_r
Cuts at: 854 1028 1419 1446 2791 854
Size: 174 391 27 1345 1332
Fragments arranged by size:
1345 1332 391 174 27
EagI C'GGCC_G
Cuts at: 854 854
Size: 3269
EarI CTCTTCn'nnn_
Cuts at: 141 1292 1502 141
Size: 1151 210 1908
Fragments arranged by size:
1908 1151 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1477 1787 1933 3079 1477
Size: 310 146 1146 1667
Fragments arranged by size:
1667 1146 310 146
EcoICRI GAG'CTC
Cuts at: 281 281
Size: 3269
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1094 1526 2273 2541 1094
Size: 432 747 268 1822
Fragments arranged by size:
1822 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 408 408
Size: 3269
EcoRI G'AATT_C
Cuts at: 273 273
Size: 3269
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1288 1288
Size: 3269
FspI TGC'GCA
Cuts at: 1050 1050
Size: 3269
HaeII r_GCGC'y
Cuts at: 703 951 1973 2952 703
Size: 248 1022 979 1020
Fragments arranged by size:
1022 1020 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1111 1141 1143 1173 1111
Size: 30 2 30 3207
Fragments arranged by size:
3207 30 30 2
HincII GTy'rAC
Cuts at: 308 308
Size: 3269
KasI G'GCGC_C
Cuts at: 699 947 699
Size: 248 3021
KpnI G_GTAC'C
Cuts at: 289 289
Size: 3269
MluI A'CGCG_T
Cuts at: 2702 2702
Size: 3269
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1940 2124 1940
Size: 184 3085
MscI TGG'CCA
Cuts at: 1030 1030
Size: 3269
MslI CAynn'nnrTG
Cuts at: 163 253 1258 1385 163
Size: 90 1005 127 2047
Fragments arranged by size:
2047 1005 127 90
MspA1I CmG'CkG
Cuts at: 380 694 1054 2067 2312 380
Size: 314 360 1013 245 1337
Fragments arranged by size:
1337 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1450 1450
Size: 3269
NarI GG'CG_CC
Cuts at: 700 948 700
Size: 248 3021
NcoI C'CATG_G
Cuts at: 107 107
Size: 3269
NgoMIV G'CCGG_C
Cuts at: 1448 1448
Size: 3269
NspI r_CATG'y
Cuts at: 322 822 1729 322
Size: 500 907 1862
Fragments arranged by size:
1862 907 500
PciI A'CATG_T
Cuts at: 1725 1725
Size: 3269
PmeI GTTT'AAAC
Cuts at: 268 268
Size: 3269
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2408 2440 2408
Size: 32 3237
PstI C_TGCA'G
Cuts at: 316 1001 316
Size: 685 2584
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3269
PvuII CAG'CTG
Cuts at: 694 1054 694
Size: 360 2909
RsrII CG'GwC_CG
Cuts at: 1464 1464
Size: 3269
SacI G_AGCT'C
Cuts at: 283 283
Size: 3269
SalI G'TCGA_C
Cuts at: 306 306
Size: 3269
SapI GCTCTTCn'nnn_
Cuts at: 141 1292 1502 141
Size: 1151 210 1908
Fragments arranged by size:
1908 1151 210
SbfI CC_TGCA'GG
Cuts at: 316 316
Size: 3269
ScaI AGT'ACT
Cuts at: 2786 2786
Size: 3269
SfcI C'TryA_G
Cuts at: 33 190 312 997 1990 2181 33
Size: 157 122 685 993 191 1121
Fragments arranged by size:
1121 993 685 191 157 122
SfoI GGC'GCC
Cuts at: 701 949 701
Size: 248 3021
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3269
SmaI CCC'GGG
Cuts at: 291 291
Size: 3269
SmlI C'TyrA_G
Cuts at: 424 1339 1831 2093 424
Size: 915 492 262 1600
Fragments arranged by size:
1600 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3269
SphI G_CATG'C
Cuts at: 322 822 322
Size: 500 2769
StyI C'CwwG_G
Cuts at: 107 403 757 107
Size: 296 354 2619
Fragments arranged by size:
2619 354 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 852 985 1444 1653 852
Size: 133 459 209 2468
Fragments arranged by size:
2468 459 209 133
TatI w'GTAC_w
Cuts at: 2784 2784
Size: 3269
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2786 2818 2786
Size: 32 3237
Tth111I GACn'n_nGTC
Cuts at: 1066 1066
Size: 3269
XbaI T'CTAG_A
Cuts at: 300 300
Size: 3269
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 764 764
Size: 3269
XmaI C'CCGG_G
Cuts at: 289 289
Size: 3269
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI