Restriction map for
pF1KB8738
(Circular) (Six-base) MAPSORT of: pF1KB8738.seq Check: 5439 from: 1 to: 3446
pF1KB8738 3446 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 484 484
Size: 3446
Acc65I G'GTAC_C
Cuts at: 462 462
Size: 3446
AclI AA'CG_TT
Cuts at: 3286 3286
Size: 3446
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1271 1703 2450 2718 1271
Size: 432 747 268 1999
Fragments arranged by size:
1999 747 432 268
AflIII A'CryG_T
Cuts at: 798 1902 2879 798
Size: 1104 977 1365
Fragments arranged by size:
1365 1104 977
AgeI A'CCGG_T
Cuts at: 1834 1834
Size: 3446
AlwNI CAG_nnn'CTG
Cuts at: 751 2318 751
Size: 1567 1879
ApaLI G'TGCA_C
Cuts at: 2216 2216
Size: 3446
ApoI r'AATT_y
Cuts at: 424 450 2799 3055 424
Size: 26 2349 256 815
Fragments arranged by size:
2349 815 256 26
AseI AT'TA_AT
Cuts at: 20 20
Size: 3446
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3446
AvaI C'yCGr_G
Cuts at: 466 466
Size: 3446
BamHI G'GATC_C
Cuts at: 471 471
Size: 3446
BanI G'GyrC_C
Cuts at: 462 876 1124 1159 462
Size: 414 248 35 2749
Fragments arranged by size:
2749 414 248 35
BanII G_rGCy'C
Cuts at: 398 460 1490 398
Size: 62 1030 2354
Fragments arranged by size:
2354 1030 62
BbeI G_GCGC'C
Cuts at: 880 1128 880
Size: 248 3198
BbsI GAAGACnn'nnnn_
Cuts at: 173 173
Size: 3446
BciVI GTATCCnnnnn_n'
Cuts at: 391 1344 2105 3446 391
Size: 953 761 1341 391
Fragments arranged by size:
1341 953 761 391
BclI T'GATC_A
Cuts at: 966 966
Size: 3446
BglII A'GATC_T
Cuts at: 961 2998 961
Size: 2037 1409
BlpI GC'TnA_GC
Cuts at: 558 558
Size: 3446
Bme1580I G_kGCm'C
Cuts at: 1071 1164 2220 1071
Size: 93 1056 2297
Fragments arranged by size:
2297 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 658 739 1074 3218 658
Size: 81 335 2144 886
Fragments arranged by size:
2144 886 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1813 1813
Size: 3446
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 1501 1993 2291 622
Size: 879 492 298 1777
Fragments arranged by size:
1777 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3150 3150
Size: 3446
BsaAI yAC'GTr
Cuts at: 799 799
Size: 3446
BsaBI GATnn'nnATC
Cuts at: 6 960 2997 6
Size: 954 2037 455
Fragments arranged by size:
2037 954 455
BsaHI Gr'CG_yC
Cuts at: 877 1125 1827 3323 877
Size: 248 702 1496 1000
Fragments arranged by size:
1496 1000 702 248
BsaWI w'CCGG_w
Cuts at: 635 1156 1834 2108 2255 2666 635
Size: 521 678 274 147 411 1415
Fragments arranged by size:
1415 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 346 376 346
Size: 30 3416
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 197 337 197
Size: 140 3306
BseYI C'CCAG_C
Cuts at: 871 1629 2206 2702 871
Size: 758 577 496 1615
Fragments arranged by size:
1615 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 988 1034 2242 104
Size: 884 46 1208 1308
Fragments arranged by size:
1308 1208 884 46
BsiHKAI G_wGCw'C
Cuts at: 398 460 1238 1428 2220 398
Size: 62 778 190 792 1624
Fragments arranged by size:
1624 792 778 190 62
Bsp1286I G_dGCh'C
Cuts at: 398 460 1071 1164 1238 1428 1490 2220 398
Size: 62 611 93 74 190 62 730 1624
Fragments arranged by size:
1624 730 611 190 93 74 62 62
BspEI T'CCGG_A
Cuts at: 2666 2666
Size: 3446
BspHI T'CATG_A
Cuts at: 417 2622 417
Size: 2205 1241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 496 710 1012 1393 496
Size: 214 302 381 2549
Fragments arranged by size:
2549 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1738 1792 3276 3441 50
Size: 1688 54 1484 165 55
Fragments arranged by size:
1688 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1358 1358
Size: 3446
BsrFI r'CCGG_y
Cuts at: 1444 1625 1834 1444
Size: 181 209 3056
Fragments arranged by size:
3056 209 181
BssSI C'ACGA_G
Cuts at: 1717 2075 1717
Size: 358 3088
BstAPI GCAn_nnn'nTGC
Cuts at: 150 823 150
Size: 673 2773
BstBI TT'CG_AA
Cuts at: 1807 1807
Size: 3446
BstYI r'GATC_y
Cuts at: 1 471 938 961 1296 1542 2554 2640 2652
Size: 470 467 23 335 246 1012 86 12
Cuts at: 2652 2998 1
Size: 346 449
Fragments arranged by size:
1012 470 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3446
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1396 1577 1740 1396
Size: 181 163 3102
Fragments arranged by size:
3102 181 163
BtsI GCAGTG_nn'
Cuts at: 689 689
Size: 3446
DraI TTT'AAA
Cuts at: 445 445
Size: 3446
DrdI GACnn_nn'nnGTC
Cuts at: 1152 2010 1152
Size: 858 2588
EaeI y'GGCC_r
Cuts at: 1031 1205 1596 1623 2968 1031
Size: 174 391 27 1345 1509
Fragments arranged by size:
1509 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1031 1031
Size: 3446
EarI CTCTTCn'nnn_
Cuts at: 128 1469 1679 128
Size: 1341 210 1895
Fragments arranged by size:
1895 1341 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1654 1964 2110 3256 1654
Size: 310 146 1146 1844
Fragments arranged by size:
1844 1146 310 146
EcoICRI GAG'CTC
Cuts at: 396 458 396
Size: 62 3384
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1271 1703 2450 2718 1271
Size: 432 747 268 1999
Fragments arranged by size:
1999 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 179 179
Size: 3446
EcoO109I rG'GnC_Cy
Cuts at: 585 585
Size: 3446
EcoRI G'AATT_C
Cuts at: 450 450
Size: 3446
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1465 1465
Size: 3446
FspI TGC'GCA
Cuts at: 1227 1227
Size: 3446
HaeII r_GCGC'y
Cuts at: 880 1128 2150 3129 880
Size: 248 1022 979 1197
Fragments arranged by size:
1197 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 357 389 1288 1318 1320 1350 357
Size: 32 899 30 2 30 2453
Fragments arranged by size:
2453 899 32 30 30 2
HincII GTy'rAC
Cuts at: 485 485
Size: 3446
KasI G'GCGC_C
Cuts at: 876 1124 876
Size: 248 3198
KpnI G_GTAC'C
Cuts at: 466 466
Size: 3446
MluI A'CGCG_T
Cuts at: 2879 2879
Size: 3446
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2117 2301 2117
Size: 184 3262
MscI TGG'CCA
Cuts at: 1207 1207
Size: 3446
MslI CAynn'nnrTG
Cuts at: 419 1435 1562 419
Size: 1016 127 2303
Fragments arranged by size:
2303 1016 127
MspA1I CmG'CkG
Cuts at: 557 871 1231 2244 2489 557
Size: 314 360 1013 245 1514
Fragments arranged by size:
1514 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1627 1627
Size: 3446
NarI GG'CG_CC
Cuts at: 877 1125 877
Size: 248 3198
NcoI C'CATG_G
Cuts at: 107 107
Size: 3446
NgoMIV G'CCGG_C
Cuts at: 1625 1625
Size: 3446
NspI r_CATG'y
Cuts at: 499 999 1906 499
Size: 500 907 2039
Fragments arranged by size:
2039 907 500
PciI A'CATG_T
Cuts at: 1902 1902
Size: 3446
PmeI GTTT'AAAC
Cuts at: 445 445
Size: 3446
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2585 2617 2585
Size: 32 3414
PstI C_TGCA'G
Cuts at: 207 362 493 1178 207
Size: 155 131 685 2475
Fragments arranged by size:
2475 685 155 131
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3446
PvuII CAG'CTG
Cuts at: 871 1231 871
Size: 360 3086
RsrII CG'GwC_CG
Cuts at: 1641 1641
Size: 3446
SacI G_AGCT'C
Cuts at: 398 460 398
Size: 62 3384
SalI G'TCGA_C
Cuts at: 483 483
Size: 3446
SapI GCTCTTCn'nnn_
Cuts at: 1469 1679 1469
Size: 210 3236
SbfI CC_TGCA'GG
Cuts at: 207 493 207
Size: 286 3160
ScaI AGT'ACT
Cuts at: 2963 2963
Size: 3446
SfcI C'TryA_G
Cuts at: 33 203 358 489 1174 2167 2358 33
Size: 170 155 131 685 993 191 1121
Fragments arranged by size:
1121 993 685 191 170 155 131
SfoI GGC'GCC
Cuts at: 878 1126 878
Size: 248 3198
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3446
SmaI CCC'GGG
Cuts at: 468 468
Size: 3446
SmlI C'TyrA_G
Cuts at: 601 1516 2008 2270 601
Size: 915 492 262 1777
Fragments arranged by size:
1777 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3446
SphI G_CATG'C
Cuts at: 499 999 499
Size: 500 2946
SspI AAT'ATT
Cuts at: 117 314 117
Size: 197 3249
StyI C'CwwG_G
Cuts at: 107 580 934 107
Size: 473 354 2619
Fragments arranged by size:
2619 473 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 277 1029 1162 1621 1830 277
Size: 752 133 459 209 1893
Fragments arranged by size:
1893 752 459 209 133
TatI w'GTAC_w
Cuts at: 2961 2961
Size: 3446
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2963 2995 2963
Size: 32 3414
Tth111I GACn'n_nGTC
Cuts at: 1243 1243
Size: 3446
XbaI T'CTAG_A
Cuts at: 477 477
Size: 3446
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 941 941
Size: 3446
XmaI C'CCGG_G
Cuts at: 466 466
Size: 3446
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI