Restriction map for
pF1KB8737
(Circular) (Six-base) MAPSORT of: pF1KB8737.seq Check: 1038 from: 1 to: 3542
pF1KB8737 3542 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 365 365
Size: 3542
AccI GT'mk_AC
Cuts at: 580 580
Size: 3542
Acc65I G'GTAC_C
Cuts at: 558 558
Size: 3542
AclI AA'CG_TT
Cuts at: 3382 3382
Size: 3542
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1367 1799 2546 2814 1367
Size: 432 747 268 2095
Fragments arranged by size:
2095 747 432 268
AflIII A'CryG_T
Cuts at: 894 1998 2975 894
Size: 1104 977 1461
Fragments arranged by size:
1461 1104 977
AgeI A'CCGG_T
Cuts at: 1930 1930
Size: 3542
AlwNI CAG_nnn'CTG
Cuts at: 847 2414 847
Size: 1567 1975
ApaLI G'TGCA_C
Cuts at: 2312 2312
Size: 3542
ApoI r'AATT_y
Cuts at: 546 2895 3151 546
Size: 2349 256 937
Fragments arranged by size:
2349 937 256
AscI GG'CGCG_CC
Cuts at: 248 248
Size: 3542
AseI AT'TA_AT
Cuts at: 20 20
Size: 3542
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3542
AvaI C'yCGr_G
Cuts at: 498 562 498
Size: 64 3478
BamHI G'GATC_C
Cuts at: 567 567
Size: 3542
BanI G'GyrC_C
Cuts at: 241 492 558 972 1220 1255 241
Size: 251 66 414 248 35 2528
Fragments arranged by size:
2528 414 251 248 66 35
BanII G_rGCy'C
Cuts at: 389 556 1586 389
Size: 167 1030 2345
Fragments arranged by size:
2345 1030 167
BbeI G_GCGC'C
Cuts at: 245 496 976 1224 245
Size: 251 480 248 2563
Fragments arranged by size:
2563 480 251 248
BbsI GAAGACnn'nnnn_
Cuts at: 340 472 340
Size: 132 3410
BciVI GTATCCnnnnn_n'
Cuts at: 1440 2201 3542 1440
Size: 761 1341 1440
Fragments arranged by size:
1440 1341 761
BclI T'GATC_A
Cuts at: 1062 1062
Size: 3542
BglI GCCn_nnn'nGGC
Cuts at: 520 520
Size: 3542
BglII A'GATC_T
Cuts at: 1057 3094 1057
Size: 2037 1505
BlpI GC'TnA_GC
Cuts at: 654 654
Size: 3542
Bme1580I G_kGCm'C
Cuts at: 1167 1260 2316 1167
Size: 93 1056 2393
Fragments arranged by size:
2393 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 181 754 835 1170 3314 181
Size: 573 81 335 2144 409
Fragments arranged by size:
2144 573 409 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1909 1909
Size: 3542
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 295 319 163
Size: 132 24 3386
Fragments arranged by size:
3386 132 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 1597 2089 2387 718
Size: 879 492 298 1873
Fragments arranged by size:
1873 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3246 3246
Size: 3542
BsaAI yAC'GTr
Cuts at: 895 895
Size: 3542
BsaBI GATnn'nnATC
Cuts at: 6 1056 3093 6
Size: 1050 2037 455
Fragments arranged by size:
2037 1050 455
BsaHI Gr'CG_yC
Cuts at: 242 428 493 973 1221 1923 3419 242
Size: 186 65 480 248 702 1496 365
Fragments arranged by size:
1496 702 480 365 248 186 65
BsaWI w'CCGG_w
Cuts at: 731 1252 1930 2204 2351 2762 731
Size: 521 678 274 147 411 1511
Fragments arranged by size:
1511 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 397 397
Size: 3542
BseYI C'CCAG_C
Cuts at: 399 967 1725 2302 2798 399
Size: 568 758 577 496 1143
Fragments arranged by size:
1143 758 577 568 496
BsiEI CG_ry'CG
Cuts at: 104 416 1084 1130 2338 104
Size: 312 668 46 1208 1308
Fragments arranged by size:
1308 1208 668 312 46
BsiHKAI G_wGCw'C
Cuts at: 389 556 1334 1524 2316 389
Size: 167 778 190 792 1615
Fragments arranged by size:
1615 792 778 190 167
Bsp1286I G_dGCh'C
Cuts at: 389 556 1167 1260 1334 1524 1586 2316 389
Size: 167 611 93 74 190 62 730 1615
Fragments arranged by size:
1615 730 611 190 167 93 74 62
BspEI T'CCGG_A
Cuts at: 2762 2762
Size: 3542
BspHI T'CATG_A
Cuts at: 2718 2718
Size: 3542
BspMI ACCTGCnnnn'nnnn_
Cuts at: 365 592 806 1108 1489 365
Size: 227 214 302 381 2418
Fragments arranged by size:
2418 381 302 227 214
BsrBI CCG'CTC
Cuts at: 50 1834 1888 3372 3537 50
Size: 1784 54 1484 165 55
Fragments arranged by size:
1784 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 318 1454 318
Size: 1136 2406
BsrFI r'CCGG_y
Cuts at: 172 203 244 1540 1721 1930 172
Size: 31 41 1296 181 209 1784
Fragments arranged by size:
1784 1296 209 181 41 31
BsrGI T'GTAC_A
Cuts at: 198 198
Size: 3542
BssHII G'CGCG_C
Cuts at: 168 248 168
Size: 80 3462
BssSI C'ACGA_G
Cuts at: 1813 2171 1813
Size: 358 3184
BstAPI GCAn_nnn'nTGC
Cuts at: 919 919
Size: 3542
BstBI TT'CG_AA
Cuts at: 1903 1903
Size: 3542
BstYI r'GATC_y
Cuts at: 1 293 567 1034 1057 1392 1638 2650 2736
Size: 292 274 467 23 335 246 1012 86
Cuts at: 2736 2748 3094 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 292 274 246
86 23 12
BtgI C'CryG_G
Cuts at: 117 479 117
Size: 362 3180
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1492 1673 1836 1492
Size: 181 163 3198
Fragments arranged by size:
3198 181 163
BtsI GCAGTG_nn'
Cuts at: 275 785 275
Size: 510 3032
DraI TTT'AAA
Cuts at: 541 541
Size: 3542
DrdI GACnn_nn'nnGTC
Cuts at: 1248 2106 1248
Size: 858 2684
EaeI y'GGCC_r
Cuts at: 365 1127 1301 1692 1719 3064 365
Size: 762 174 391 27 1345 843
Fragments arranged by size:
1345 843 762 391 174 27
EagI C'GGCC_G
Cuts at: 1127 1127
Size: 3542
EarI CTCTTCn'nnn_
Cuts at: 1565 1775 1565
Size: 210 3332
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1750 2060 2206 3352 1750
Size: 310 146 1146 1940
Fragments arranged by size:
1940 1146 310 146
EcoICRI GAG'CTC
Cuts at: 387 554 387
Size: 167 3375
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 295 319 1367 1799 2546 2814 163
Size: 132 24 1048 432 747 268 891
Fragments arranged by size:
1048 891 747 432 268 132 24
EcoO109I rG'GnC_Cy
Cuts at: 681 681
Size: 3542
EcoRI G'AATT_C
Cuts at: 546 546
Size: 3542
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1561 1561
Size: 3542
FspI TGC'GCA
Cuts at: 1323 1323
Size: 3542
HaeII r_GCGC'y
Cuts at: 245 476 496 976 1224 2246 3225 245
Size: 231 20 480 248 1022 979 562
Fragments arranged by size:
1022 979 562 480 248 231 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1384 1414 1416 1446 1384
Size: 30 2 30 3480
Fragments arranged by size:
3480 30 30 2
HincII GTy'rAC
Cuts at: 581 581
Size: 3542
KasI G'GCGC_C
Cuts at: 241 492 972 1220 241
Size: 251 480 248 2563
Fragments arranged by size:
2563 480 251 248
KpnI G_GTAC'C
Cuts at: 562 562
Size: 3542
MluI A'CGCG_T
Cuts at: 2975 2975
Size: 3542
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2213 2397 2213
Size: 184 3358
MscI TGG'CCA
Cuts at: 367 1303 367
Size: 936 2606
MslI CAynn'nnrTG
Cuts at: 1531 1658 1531
Size: 127 3415
MspA1I CmG'CkG
Cuts at: 119 289 363 653 967 1327 2340 2585 119
Size: 170 74 290 314 360 1013 245 1076
Fragments arranged by size:
1076 1013 360 314 290 245 170 74
NaeI GCC'GGC
Cuts at: 174 246 1723 174
Size: 72 1477 1993
Fragments arranged by size:
1993 1477 72
NarI GG'CG_CC
Cuts at: 242 493 973 1221 242
Size: 251 480 248 2563
Fragments arranged by size:
2563 480 251 248
NgoMIV G'CCGG_C
Cuts at: 172 244 1721 172
Size: 72 1477 1993
Fragments arranged by size:
1993 1477 72
NspI r_CATG'y
Cuts at: 595 1095 2002 595
Size: 500 907 2135
Fragments arranged by size:
2135 907 500
PasI CC'CwG_GG
Cuts at: 420 420
Size: 3542
PciI A'CATG_T
Cuts at: 1998 1998
Size: 3542
PfoI T'CCnGG_A
Cuts at: 296 296
Size: 3542
PmeI GTTT'AAAC
Cuts at: 541 541
Size: 3542
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2681 2713 2681
Size: 32 3510
PstI C_TGCA'G
Cuts at: 362 589 1274 362
Size: 227 685 2630
Fragments arranged by size:
2630 685 227
PvuI CG_AT'CG
Cuts at: 104 416 104
Size: 312 3230
PvuII CAG'CTG
Cuts at: 363 967 1327 363
Size: 604 360 2578
Fragments arranged by size:
2578 604 360
RsrII CG'GwC_CG
Cuts at: 1737 1737
Size: 3542
SacI G_AGCT'C
Cuts at: 389 556 389
Size: 167 3375
SacII CC_GC'GG
Cuts at: 120 120
Size: 3542
SalI G'TCGA_C
Cuts at: 579 579
Size: 3542
SapI GCTCTTCn'nnn_
Cuts at: 1565 1775 1565
Size: 210 3332
SbfI CC_TGCA'GG
Cuts at: 589 589
Size: 3542
ScaI AGT'ACT
Cuts at: 3059 3059
Size: 3542
SfcI C'TryA_G
Cuts at: 33 358 585 1270 2263 2454 33
Size: 325 227 685 993 191 1121
Fragments arranged by size:
1121 993 685 325 227 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 520 520
Size: 3542
SfoI GGC'GCC
Cuts at: 243 494 974 1222 243
Size: 251 480 248 2563
Fragments arranged by size:
2563 480 251 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3542
SgrAI Cr'CCGG_yG
Cuts at: 244 244
Size: 3542
SmaI CCC'GGG
Cuts at: 564 564
Size: 3542
SmlI C'TyrA_G
Cuts at: 697 1612 2104 2366 697
Size: 915 492 262 1873
Fragments arranged by size:
1873 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3542
SphI G_CATG'C
Cuts at: 595 1095 595
Size: 500 3042
StuI AGG'CCT
Cuts at: 523 523
Size: 3542
StyI C'CwwG_G
Cuts at: 146 676 1030 146
Size: 530 354 2658
Fragments arranged by size:
2658 530 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 532 1125 1258 1717 1926 532
Size: 593 133 459 209 2148
Fragments arranged by size:
2148 593 459 209 133
TatI w'GTAC_w
Cuts at: 198 3057 198
Size: 2859 683
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3059 3091 3059
Size: 32 3510
Tth111I GACn'n_nGTC
Cuts at: 1339 1339
Size: 3542
XbaI T'CTAG_A
Cuts at: 573 573
Size: 3542
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 477 1037 477
Size: 560 2982
XmaI C'CCGG_G
Cuts at: 562 562
Size: 3542
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI