Restriction map for
pF1KB8734
(Circular) (Six-base) MAPSORT of: pF1KB8734.seq Check: 8887 from: 1 to: 4766
pF1KB8734 4766 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 120 120
Size: 4766
AccI GT'mk_AC
Cuts at: 1804 1804
Size: 4766
Acc65I G'GTAC_C
Cuts at: 1782 1782
Size: 4766
AclI AA'CG_TT
Cuts at: 1108 4606 1108
Size: 3498 1268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 947 2591 3023 3376 4038 535
Size: 412 1644 432 353 662 1263
Fragments arranged by size:
1644 1263 662 432 412 353
AflII C'TTAA_G
Cuts at: 1395 1395
Size: 4766
AflIII A'CryG_T
Cuts at: 2118 3918 4199 2118
Size: 1800 281 2685
Fragments arranged by size:
2685 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 925 2071 3509 925
Size: 1146 1438 2182
Fragments arranged by size:
2182 1438 1146
ApaLI G'TGCA_C
Cuts at: 3604 3604
Size: 4766
ApoI r'AATT_y
Cuts at: 1770 4119 4375 1770
Size: 2349 256 2161
Fragments arranged by size:
2349 2161 256
AseI AT'TA_AT
Cuts at: 20 652 20
Size: 632 4134
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4766
AvaI C'yCGr_G
Cuts at: 1786 1786
Size: 4766
AvrII C'CTAG_G
Cuts at: 286 391 286
Size: 105 4661
BamHI G'GATC_C
Cuts at: 1134 1791 1134
Size: 657 4109
BanI G'GyrC_C
Cuts at: 149 1782 2196 2444 2479 149
Size: 1633 414 248 35 2436
Fragments arranged by size:
2436 1633 414 248 35
BanII G_rGCy'C
Cuts at: 1780 2810 1780
Size: 1030 3736
BbeI G_GCGC'C
Cuts at: 2200 2448 2200
Size: 248 4518
BbsI GAAGACnn'nnnn_
Cuts at: 255 255
Size: 4766
BciVI GTATCCnnnnn_n'
Cuts at: 2664 3720 4766 2664
Size: 1056 1046 2664
Fragments arranged by size:
2664 1056 1046
BclI T'GATC_A
Cuts at: 2286 2286
Size: 4766
BglII A'GATC_T
Cuts at: 1281 2281 4318 1281
Size: 1000 2037 1729
Fragments arranged by size:
2037 1729 1000
BlpI GC'TnA_GC
Cuts at: 1878 1878
Size: 4766
Bme1580I G_kGCm'C
Cuts at: 152 2391 2484 3608 152
Size: 2239 93 1124 1310
Fragments arranged by size:
2239 1310 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1187 1978 2059 2394 4538 1187
Size: 791 81 335 2144 1415
Fragments arranged by size:
2144 1415 791 335 81
BmtI G_CTAG'C
Cuts at: 116 116
Size: 4766
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3133 3133
Size: 4766
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 672 1191 1197 1203 1209 160
Size: 512 519 6 6 6 3717
Fragments arranged by size:
3717 519 512 6 6 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1942 2821 3535 3833 1942
Size: 879 714 298 2875
Fragments arranged by size:
2875 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4470 4470
Size: 4766
BsaAI yAC'GTr
Cuts at: 2119 2119
Size: 4766
BsaBI GATnn'nnATC
Cuts at: 6 1691 2280 4317 6
Size: 1685 589 2037 455
Fragments arranged by size:
2037 1685 589 455
BsaHI Gr'CG_yC
Cuts at: 2197 2445 3147 4643 2197
Size: 248 702 1496 2320
Fragments arranged by size:
2320 1496 702 248
BsaWI w'CCGG_w
Cuts at: 222 1955 2476 3154 3565 3712 3986 222
Size: 1733 521 678 411 147 274 1002
Fragments arranged by size:
1733 1002 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1171 1318 1382 1171
Size: 147 64 4555
Fragments arranged by size:
4555 147 64
BseYI C'CCAG_C
Cuts at: 144 2191 2949 3614 4022 144
Size: 2047 758 665 408 888
Fragments arranged by size:
2047 888 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 148
Size: 4766
BsiEI CG_ry'CG
Cuts at: 104 2308 2354 3584 104
Size: 2204 46 1230 1286
Fragments arranged by size:
2204 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 230 665 1780 2558 2748 3608 230
Size: 435 1115 778 190 860 1388
Fragments arranged by size:
1388 1115 860 778 435 190
BsiWI C'GTAC_G
Cuts at: 213 213
Size: 4766
BsmI GAATG_Cn'
Cuts at: 1287 1287
Size: 4766
Bsp1286I G_dGCh'C
Cuts at: 152 230 665 1780 2391 2484 2558 2748 2810
Size: 78 435 1115 611 93 74 190 62
Cuts at: 2810 3608 152
Size: 798 1310
Fragments arranged by size:
1310 1115 798 611 435 190 93 78
74 62
BspEI T'CCGG_A
Cuts at: 222 222
Size: 4766
BspHI T'CATG_A
Cuts at: 3198 3198
Size: 4766
BspMI ACCTGCnnnn'nnnn_
Cuts at: 120 1816 2030 2332 2713 120
Size: 1696 214 302 381 2173
Fragments arranged by size:
2173 1696 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3058 3112 4596 4761 50
Size: 3008 54 1484 165 55
Fragments arranged by size:
3008 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2678 2678
Size: 4766
BsrFI r'CCGG_y
Cuts at: 2764 2945 2764
Size: 181 4585
BssSI C'ACGA_G
Cuts at: 117 3037 3745 117
Size: 2920 708 1138
Fragments arranged by size:
2920 1138 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2143 2143
Size: 4766
BstBI TT'CG_AA
Cuts at: 3127 3127
Size: 4766
BstYI r'GATC_y
Cuts at: 1 309 1134 1281 1791 2258 2281 2616 2862
Size: 308 825 147 510 467 23 335 246
Cuts at: 2862 3168 3180 3266 4318 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 825 510 467 449 335 308 306
246 147 86 23 12
BtgI C'CryG_G
Cuts at: 107 179 107
Size: 72 4694
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2716 2897 3060 2716
Size: 181 163 4422
Fragments arranged by size:
4422 181 163
BtsI GCAGTG_nn'
Cuts at: 2009 2009
Size: 4766
DraI TTT'AAA
Cuts at: 375 978 1765 375
Size: 603 787 3376
Fragments arranged by size:
3376 787 603
DraIII CAC_nnn'GTG
Cuts at: 249 249
Size: 4766
DrdI GACnn_nn'nnGTC
Cuts at: 2472 3816 2472
Size: 1344 3422
EaeI y'GGCC_r
Cuts at: 2351 2525 2916 2943 4288 2351
Size: 174 391 27 1345 2829
Fragments arranged by size:
2829 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2351 2351
Size: 4766
EarI CTCTTCn'nnn_
Cuts at: 782 2789 2999 782
Size: 2007 210 2549
Fragments arranged by size:
2549 2007 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2974 3716 3862 4576 2974
Size: 742 146 714 3164
Fragments arranged by size:
3164 742 714 146
EcoICRI GAG'CTC
Cuts at: 1778 1778
Size: 4766
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 535 672 947 1191 1197 1203 1209 2591
Size: 375 137 275 244 6 6 6 1382
Cuts at: 2591 3023 3376 4038 160
Size: 432 353 662 888
Fragments arranged by size:
1382 888 662 432 375 353 275 244
137 6 6 6
EcoO109I rG'GnC_Cy
Cuts at: 528 1258 1905 528
Size: 730 647 3389
Fragments arranged by size:
3389 730 647
EcoRI G'AATT_C
Cuts at: 1770 1770
Size: 4766
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 828 2785 828
Size: 1957 2809
FspI TGC'GCA
Cuts at: 2547 2547
Size: 4766
HaeII r_GCGC'y
Cuts at: 2200 2448 3678 4449 2200
Size: 248 1230 771 2517
Fragments arranged by size:
2517 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1150 1182 1294 1324 1326 1356 1684 1716 2608
Size: 32 112 30 2 30 328 32 892
Cuts at: 2608 2638 2640 2670 1150
Size: 30 2 30 3246
Fragments arranged by size:
3246 892 328 112 32 32 30 30
30 30 2 2
HincII GTy'rAC
Cuts at: 1805 1805
Size: 4766
HindIII A'AGCT_T
Cuts at: 279 279
Size: 4766
KasI G'GCGC_C
Cuts at: 2196 2444 2196
Size: 248 4518
KpnI G_GTAC'C
Cuts at: 1786 1786
Size: 4766
MfeI C'AATT_G
Cuts at: 359 359
Size: 4766
MluI A'CGCG_T
Cuts at: 4199 4199
Size: 4766
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3525 3709 3525
Size: 184 4582
MscI TGG'CCA
Cuts at: 2527 2527
Size: 4766
MslI CAynn'nnrTG
Cuts at: 701 1292 2755 2882 701
Size: 591 1463 127 2585
Fragments arranged by size:
2585 1463 591 127
MspA1I CmG'CkG
Cuts at: 461 1877 2191 2551 3335 3580 461
Size: 1416 314 360 784 245 1647
Fragments arranged by size:
1647 1416 784 360 314 245
NaeI GCC'GGC
Cuts at: 2947 2947
Size: 4766
NarI GG'CG_CC
Cuts at: 2197 2445 2197
Size: 248 4518
NcoI C'CATG_G
Cuts at: 107 107
Size: 4766
NgoMIV G'CCGG_C
Cuts at: 2945 2945
Size: 4766
NheI G'CTAG_C
Cuts at: 112 112
Size: 4766
NspI r_CATG'y
Cuts at: 1819 2319 3922 1819
Size: 500 1603 2663
Fragments arranged by size:
2663 1603 500
PciI A'CATG_T
Cuts at: 3918 3918
Size: 4766
PmeI GTTT'AAAC
Cuts at: 1765 1765
Size: 4766
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3212 3244 3212
Size: 32 4734
PpuMI rG'GwC_Cy
Cuts at: 528 1258 528
Size: 730 4036
PstI C_TGCA'G
Cuts at: 1628 1813 2498 1628
Size: 185 685 3896
Fragments arranged by size:
3896 685 185
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4766
PvuII CAG'CTG
Cuts at: 2191 2551 2191
Size: 360 4406
RsrII CG'GwC_CG
Cuts at: 220 2961 220
Size: 2741 2025
SacI G_AGCT'C
Cuts at: 1780 1780
Size: 4766
SalI G'TCGA_C
Cuts at: 1803 1803
Size: 4766
SanDI GG'GwC_CC
Cuts at: 528 528
Size: 4766
SapI GCTCTTCn'nnn_
Cuts at: 2789 2999 2789
Size: 210 4556
SbfI CC_TGCA'GG
Cuts at: 1813 1813
Size: 4766
ScaI AGT'ACT
Cuts at: 4283 4283
Size: 4766
SfcI C'TryA_G
Cuts at: 33 1624 1729 1809 2494 3462 3653 33
Size: 1591 105 80 685 968 191 1146
Fragments arranged by size:
1591 1146 968 685 191 105 80
SfoI GGC'GCC
Cuts at: 2198 2446 2198
Size: 248 4518
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4766
SmaI CCC'GGG
Cuts at: 1788 1788
Size: 4766
SmlI C'TyrA_G
Cuts at: 1395 1921 2836 3550 3812 1395
Size: 526 915 714 262 2349
Fragments arranged by size:
2349 915 714 526 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4766
SphI G_CATG'C
Cuts at: 1819 2319 1819
Size: 500 4266
StuI AGG'CCT
Cuts at: 1356 1356
Size: 4766
StyI C'CwwG_G
Cuts at: 107 286 391 1900 2254 107
Size: 179 105 1509 354 2619
Fragments arranged by size:
2619 1509 354 179 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 208 2349 2482 2941 3150 208
Size: 2141 133 459 209 1824
Fragments arranged by size:
2141 1824 459 209 133
TatI w'GTAC_w
Cuts at: 4281 4281
Size: 4766
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4283 4315 4283
Size: 32 4734
Tth111I GACn'n_nGTC
Cuts at: 2563 2563
Size: 4766
XbaI T'CTAG_A
Cuts at: 1797 1797
Size: 4766
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2261 2261
Size: 4766
XmaI C'CCGG_G
Cuts at: 1786 1786
Size: 4766
XmnI GAAnn'nnTTC
Cuts at: 281 281
Size: 4766
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AleI AlfI AloI ApaI
AscI BaeI BbvCI BcgI BfrBI BglI BmgBI Bpu10I
BsaXI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HpaI NdeI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI