Restriction map for
pF1KB8729
(Circular) (Six-base) MAPSORT of: pF1KB8729.seq Check: 8579 from: 1 to: 5636
pF1KB8729 5636 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 124 370 690 975 1535 2459 124
Size: 246 320 285 560 924 3301
Fragments arranged by size:
3301 924 560 320 285 246
AatII G_ACGT'C
Cuts at: 712 1426 1877 712
Size: 714 451 4471
Fragments arranged by size:
4471 714 451
AccI GT'mk_AC
Cuts at: 1877 2134 2674 1877
Size: 257 540 4839
Fragments arranged by size:
4839 540 257
Acc65I G'GTAC_C
Cuts at: 2652 2652
Size: 5636
AclI AA'CG_TT
Cuts at: 5476 5476
Size: 5636
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 1807 2131 3461 3893 4246 4908 706
Size: 1101 324 1330 432 353 662 1434
Fragments arranged by size:
1434 1330 1101 662 432 353 324
AfeI AGC'GCT
Cuts at: 2572 2572
Size: 5636
AflIII A'CryG_T
Cuts at: 974 2988 4788 5069 974
Size: 2014 1800 281 1541
Fragments arranged by size:
2014 1800 1541 281
AhdI GACnn_n'nnGTC
Cuts at: 1158 2163 1158
Size: 1005 4631
AleI CACnn'nnGTG
Cuts at: 524 2551 524
Size: 2027 3609
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2185 2185
Size: 5636
AlwNI CAG_nnn'CTG
Cuts at: 279 705 1614 1947 2941 4379 279
Size: 426 909 333 994 1438 1536
Fragments arranged by size:
1536 1438 994 909 426 333
ApaI G_GGCC'C
Cuts at: 1387 2221 2427 1387
Size: 834 206 4596
Fragments arranged by size:
4596 834 206
ApaLI G'TGCA_C
Cuts at: 1288 4474 1288
Size: 3186 2450
ApoI r'AATT_y
Cuts at: 1915 2640 4989 5245 1915
Size: 725 2349 256 2306
Fragments arranged by size:
2349 2306 725 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5636
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5636
AvaI C'yCGr_G
Cuts at: 587 968 1246 1338 2656 587
Size: 381 278 92 1318 3567
Fragments arranged by size:
3567 1318 381 278 92
BamHI G'GATC_C
Cuts at: 1127 1682 2661 1127
Size: 555 979 4102
Fragments arranged by size:
4102 979 555
BanI G'GyrC_C
Cuts at: 204 559 1523 2053 2370 2613 2652 3066 3314
Size: 355 964 530 317 243 39 414 248
Cuts at: 3314 3349 204
Size: 35 2491
Fragments arranged by size:
2491 964 530 414 355 317 248 243
39 35
BanII G_rGCy'C
Cuts at: 469 1387 1834 2221 2427 2603 2650 3680 469
Size: 918 447 387 206 176 47 1030 2425
Fragments arranged by size:
2425 1030 918 447 387 206 176 47
BbeI G_GCGC'C
Cuts at: 563 2374 3070 3318 563
Size: 1811 696 248 2881
Fragments arranged by size:
2881 1811 696 248
BbsI GAAGACnn'nnnn_
Cuts at: 1311 1311
Size: 5636
BbvCI CC'TCA_GC
Cuts at: 2023 2620 2023
Size: 597 5039
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2332 2366 2332
Size: 34 5602
BciVI GTATCCnnnnn_n'
Cuts at: 2142 3534 4590 5636 2142
Size: 1392 1056 1046 2142
Fragments arranged by size:
2142 1392 1056 1046
BclI T'GATC_A
Cuts at: 1895 3156 1895
Size: 1261 4375
BglI GCCn_nnn'nGGC
Cuts at: 696 1696 696
Size: 1000 4636
BglII A'GATC_T
Cuts at: 1079 1853 3151 5188 1079
Size: 774 1298 2037 1527
Fragments arranged by size:
2037 1527 1298 774
BlpI GC'TnA_GC
Cuts at: 1462 2748 1462
Size: 1286 4350
Bme1580I G_kGCm'C
Cuts at: 209 1292 1387 2221 2427 2618 3261 3354 4478
Size: 1083 95 834 206 191 643 93 1124
Cuts at: 4478 209
Size: 1367
Fragments arranged by size:
1367 1124 1083 834 643 206 191 95
93
BmrI ACTGGGnnnn_n'
Cuts at: 1592 2848 2929 3264 5408 1592
Size: 1256 81 335 2144 1820
Fragments arranged by size:
2144 1820 1256 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 600 4003 600
Size: 3403 2233
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 450 549 1522 1561 388
Size: 62 99 973 39 4463
Fragments arranged by size:
4463 973 99 62 39
Bpu10I CC'TnA_GC
Cuts at: 2023 2620 2023
Size: 597 5039
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 940 1195 1888 2512 2812 3691 4405 4703 940
Size: 255 693 624 300 879 714 298 1873
Fragments arranged by size:
1873 879 714 693 624 300 298 255
BsaI GGTCTCn'nnnn_
Cuts at: 5340 5340
Size: 5636
BsaAI yAC'GTr
Cuts at: 546 1590 2989 546
Size: 1044 1399 3193
Fragments arranged by size:
3193 1399 1044
BsaBI GATnn'nnATC
Cuts at: 6 295 3150 5187 6
Size: 289 2855 2037 455
Fragments arranged by size:
2855 2037 455 289
BsaHI Gr'CG_yC
Cuts at: 560 709 1423 1874 2371 3067 3315 4017 5513
Size: 149 714 451 497 696 248 702 1496
Cuts at: 5513 560
Size: 683
Fragments arranged by size:
1496 714 702 696 683 497 451 248
149
BsaWI w'CCGG_w
Cuts at: 2825 3346 4024 4435 4582 4856 2825
Size: 521 678 411 147 274 3605
Fragments arranged by size:
3605 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 163 193 1018 1048 163
Size: 30 825 30 4751
Fragments arranged by size:
4751 825 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1648 1648
Size: 5636
BseYI C'CCAG_C
Cuts at: 458 691 2061 2116 2220 2269 2287 3061 3819
Size: 233 1370 55 104 49 18 774 758
Cuts at: 3819 4484 4892 458
Size: 665 408 1202
Fragments arranged by size:
1370 1202 774 758 665 408 233 104
55 49 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 502 526 844 891 1570 1612 1707 1842
Size: 192 24 318 47 679 42 95 135
Cuts at: 1842 2446 2484 310
Size: 604 38 3462
Fragments arranged by size:
3462 679 604 318 192 135 95 47
42 38 24
BsiEI CG_ry'CG
Cuts at: 104 741 1166 1842 3178 3224 4454 104
Size: 637 425 676 1336 46 1230 1286
Fragments arranged by size:
1336 1286 1230 676 637 425 46
BsiHKAI G_wGCw'C
Cuts at: 190 314 347 469 1292 1472 2650 3428 3618
Size: 124 33 122 823 180 1178 778 190
Cuts at: 3618 4478 190
Size: 860 1348
Fragments arranged by size:
1348 1178 860 823 778 190 180 124
122 33
BsmI GAATG_Cn'
Cuts at: 2260 2494 2260
Size: 234 5402
Bsp1286I G_dGCh'C
Cuts at: 190 209 314 347 469 1292 1387 1472 1834
Size: 19 105 33 122 823 95 85 362
Cuts at: 1834 2221 2427 2603 2618 2650 3261 3354 3428
Size: 387 206 176 15 32 611 93 74
Cuts at: 3428 3618 3680 4478 190
Size: 190 62 798 1348
Fragments arranged by size:
1348 823 798 611 387 362 206 190
176 122 105 95 93 85 74 62
33 32 19 15
BspHI T'CATG_A
Cuts at: 1892 4068 1892
Size: 2176 3460
BspMI ACCTGCnnnn'nnnn_
Cuts at: 124 266 332 370 690 975 1535 1656 1997
Size: 142 66 38 320 285 560 121 341
Cuts at: 1997 2459 2686 2900 3202 3583 124
Size: 462 227 214 302 381 2177
Fragments arranged by size:
2177 560 462 381 341 320 302 285
227 214 142 121 66 38
BsrBI CCG'CTC
Cuts at: 50 3928 3982 5466 5631 50
Size: 3878 54 1484 165 55
Fragments arranged by size:
3878 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3548 3548
Size: 5636
BsrFI r'CCGG_y
Cuts at: 728 769 1292 1356 1558 1841 3634 3815 728
Size: 41 523 64 202 283 1793 181 2549
Fragments arranged by size:
2549 1793 523 283 202 181 64 41
BssSI C'ACGA_G
Cuts at: 349 1630 3907 4615 349
Size: 1281 2277 708 1370
Fragments arranged by size:
2277 1370 1281 708
BstAPI GCAn_nnn'nTGC
Cuts at: 194 1947 1959 2498 2507 3013 194
Size: 1753 12 539 9 506 2817
Fragments arranged by size:
2817 1753 539 506 12 9
BstBI TT'CG_AA
Cuts at: 3997 3997
Size: 5636
BstEII G'GTnAC_C
Cuts at: 669 1554 1728 669
Size: 885 174 4577
Fragments arranged by size:
4577 885 174
BstXI CCAn_nnnn'nTGG
Cuts at: 1149 1149
Size: 5636
BstYI r'GATC_y
Cuts at: 1 881 1079 1127 1682 1853 2198 2661 3128
Size: 880 198 48 555 171 345 463 467
Cuts at: 3128 3151 3486 3732 4038 4050 4136 5188 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 880 555 467 463 449 345 335
306 246 198 171 86 48 23 12
BstZ17I GTA'TAC
Cuts at: 2135 2135
Size: 5636
BtgI C'CryG_G
Cuts at: 251 632 794 1088 1166 1310 1331 1379 1452
Size: 381 162 294 78 144 21 48 73
Cuts at: 1452 1487 2480 2546 251
Size: 35 993 66 3341
Fragments arranged by size:
3341 993 381 294 162 144 78 73
66 48 35 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 225 281 744 1752 2225 3586 3767 3930 225
Size: 56 463 1008 473 1361 181 163 1931
Fragments arranged by size:
1931 1361 1008 473 463 181 163 56
BtsI GCAGTG_nn'
Cuts at: 192 1300 1936 1969 2394 2566 2879 192
Size: 1108 636 33 425 172 313 2949
Fragments arranged by size:
2949 1108 636 425 313 172 33
DraI TTT'AAA
Cuts at: 2635 2635
Size: 5636
DraIII CAC_nnn'GTG
Cuts at: 1287 2507 1287
Size: 1220 4416
DrdI GACnn_nn'nnGTC
Cuts at: 1276 3342 4686 1276
Size: 2066 1344 2226
Fragments arranged by size:
2226 2066 1344
EaeI y'GGCC_r
Cuts at: 124 738 752 791 1136 1163 1217 1313 1358
Size: 614 14 39 345 27 54 96 45
Cuts at: 1358 1688 1994 2138 2364 3221 3395 3786 3813
Size: 330 306 144 226 857 174 391 27
Cuts at: 3813 5158 124
Size: 1345 602
Fragments arranged by size:
1345 857 614 602 391 345 330 306
226 174 144 96 54 45 39 27
27 14
EagI C'GGCC_G
Cuts at: 738 1163 3221 738
Size: 425 2058 3153
Fragments arranged by size:
3153 2058 425
EarI CTCTTCn'nnn_
Cuts at: 1537 1765 2179 3659 3869 1537
Size: 228 414 1480 210 3304
Fragments arranged by size:
3304 1480 414 228 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 454 814 1140 1462 2167 3844 4586 4732 5446
Size: 360 326 322 705 1677 742 146 714
Cuts at: 5446 454
Size: 644
Fragments arranged by size:
1677 742 714 705 644 360 326 322
146
EcoICRI GAG'CTC
Cuts at: 467 2648 467
Size: 2181 3455
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 450 549 706 1522 1561 1807 2131 3461
Size: 62 99 157 816 39 246 324 1330
Cuts at: 3461 3893 4246 4908 388
Size: 432 353 662 1116
Fragments arranged by size:
1330 1116 816 662 432 353 324 246
157 99 62 39
EcoNI CCTnn'n_nnAGG
Cuts at: 484 1042 1663 2502 484
Size: 558 621 839 3618
Fragments arranged by size:
3618 839 621 558
EcoO109I rG'GnC_Cy
Cuts at: 276 321 492 1384 1668 2103 2217 2423 2424
Size: 45 171 892 284 435 114 206 1
Cuts at: 2424 2775 276
Size: 351 3137
Fragments arranged by size:
3137 892 435 351 284 206 171 114
45 1
EcoRI G'AATT_C
Cuts at: 2640 2640
Size: 5636
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3655 3655
Size: 5636
FspI TGC'GCA
Cuts at: 262 1042 3417 262
Size: 780 2375 2481
Fragments arranged by size:
2481 2375 780
HaeII r_GCGC'y
Cuts at: 563 1174 1371 1519 1948 2245 2374 2474 2574
Size: 611 197 148 429 297 129 100 100
Cuts at: 2574 3070 3318 4548 5319 563
Size: 496 248 1230 771 880
Fragments arranged by size:
1230 880 771 611 496 429 297 248
197 148 129 100 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 313 345 1018 1050 1144 1176 2041
Size: 32 118 32 673 32 94 32 865
Cuts at: 2041 2073 2149 2181 2190 2222 3478 3508 3510
Size: 32 76 32 9 32 1256 30 2
Cuts at: 3510 3540 163
Size: 30 2259
Fragments arranged by size:
2259 1256 865 673 118 94 76 32
32 32 32 32 32 32 30 30
9 2
HincII GTy'rAC
Cuts at: 1878 2675 1878
Size: 797 4839
KasI G'GCGC_C
Cuts at: 559 2370 3066 3314 559
Size: 1811 696 248 2881
Fragments arranged by size:
2881 1811 696 248
KpnI G_GTAC'C
Cuts at: 2656 2656
Size: 5636
MluI A'CGCG_T
Cuts at: 5069 5069
Size: 5636
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 959 4395 4579 959
Size: 3436 184 2016
Fragments arranged by size:
3436 2016 184
MscI TGG'CCA
Cuts at: 793 1219 1690 1996 3397 793
Size: 426 471 306 1401 3032
Fragments arranged by size:
3032 1401 471 426 306
MslI CAynn'nnrTG
Cuts at: 524 787 2551 3625 3752 524
Size: 263 1764 1074 127 2408
Fragments arranged by size:
2408 1764 1074 263 127
MspA1I CmG'CkG
Cuts at: 130 662 695 1333 1381 1500 1654 2120 2277
Size: 532 33 638 48 119 154 466 157
Cuts at: 2277 2747 3061 3421 4205 4450 130
Size: 470 314 360 784 245 1316
Fragments arranged by size:
1316 784 638 532 470 466 360 314
245 157 154 119 48 33
NaeI GCC'GGC
Cuts at: 730 1358 3817 730
Size: 628 2459 2549
Fragments arranged by size:
2549 2459 628
NarI GG'CG_CC
Cuts at: 560 2371 3067 3315 560
Size: 1811 696 248 2881
Fragments arranged by size:
2881 1811 696 248
NcoI C'CATG_G
Cuts at: 251 632 1310 1487 251
Size: 381 678 177 4400
Fragments arranged by size:
4400 678 381 177
NgoMIV G'CCGG_C
Cuts at: 728 1356 3815 728
Size: 628 2459 2549
Fragments arranged by size:
2549 2459 628
NotI GC'GGCC_GC
Cuts at: 738 738
Size: 5636
NruI TCG'CGA
Cuts at: 2210 2210
Size: 5636
NspI r_CATG'y
Cuts at: 978 2070 2166 2689 3189 4792 978
Size: 1092 96 523 500 1603 1822
Fragments arranged by size:
1822 1603 1092 523 500 96
PasI CC'CwG_GG
Cuts at: 317 317
Size: 5636
PciI A'CATG_T
Cuts at: 974 4788 974
Size: 3814 1822
PflMI CCAn_nnn'nTGG
Cuts at: 300 1500 2195 300
Size: 1200 695 3741
Fragments arranged by size:
3741 1200 695
PfoI T'CCnGG_A
Cuts at: 299 299
Size: 5636
PmeI GTTT'AAAC
Cuts at: 2635 2635
Size: 5636
PmlI CAC'GTG
Cuts at: 546 1590 546
Size: 1044 4592
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4082 4114 4082
Size: 32 5604
PpuMI rG'GwC_Cy
Cuts at: 276 321 492 1668 276
Size: 45 171 1176 4244
Fragments arranged by size:
4244 1176 171 45
PspOMI G'GGCC_C
Cuts at: 1383 2217 2423 1383
Size: 834 206 4596
Fragments arranged by size:
4596 834 206
PstI C_TGCA'G
Cuts at: 1307 1664 1943 1952 2336 2360 2405 2683 3368
Size: 357 279 9 384 24 45 278 685
Cuts at: 3368 1307
Size: 3575
Fragments arranged by size:
3575 685 384 357 279 278 45 24
9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5636
PvuII CAG'CTG
Cuts at: 2120 2277 3061 3421 2120
Size: 157 784 360 4335
Fragments arranged by size:
4335 784 360 157
RsrII CG'GwC_CG
Cuts at: 3831 3831
Size: 5636
SacI G_AGCT'C
Cuts at: 469 2650 469
Size: 2181 3455
SacII CC_GC'GG
Cuts at: 1334 1382 1334
Size: 48 5588
SalI G'TCGA_C
Cuts at: 1876 2673 1876
Size: 797 4839
SanDI GG'GwC_CC
Cuts at: 492 492
Size: 5636
SapI GCTCTTCn'nnn_
Cuts at: 1537 1765 2179 3659 3869 1537
Size: 228 414 1480 210 3304
Fragments arranged by size:
3304 1480 414 228 210
SbfI CC_TGCA'GG
Cuts at: 2683 2683
Size: 5636
ScaI AGT'ACT
Cuts at: 1546 5153 1546
Size: 3607 2029
SexAI A'CCwGG_T
Cuts at: 2503 2503
Size: 5636
SfcI C'TryA_G
Cuts at: 33 1303 1323 1660 1939 1948 2332 2356 2401
Size: 1270 20 337 279 9 384 24 45
Cuts at: 2401 2679 3364 4332 4523 33
Size: 278 685 968 191 1146
Fragments arranged by size:
1270 1146 968 685 384 337 279 278
191 45 24 20 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 1696 1696
Size: 5636
SfoI GGC'GCC
Cuts at: 561 2372 3068 3316 561
Size: 1811 696 248 2881
Fragments arranged by size:
2881 1811 696 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5636
SgrAI Cr'CCGG_yG
Cuts at: 769 769
Size: 5636
SmaI CCC'GGG
Cuts at: 970 2658 970
Size: 1688 3948
SmlI C'TyrA_G
Cuts at: 955 1210 1903 2527 2791 3706 4420 4682 955
Size: 255 693 624 264 915 714 262 1909
Fragments arranged by size:
1909 915 714 693 624 264 262 255
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5636
SphI G_CATG'C
Cuts at: 2070 2689 3189 2070
Size: 619 500 4517
Fragments arranged by size:
4517 619 500
StuI AGG'CCT
Cuts at: 1344 1699 2497 1344
Size: 355 798 4483
Fragments arranged by size:
4483 798 355
StyI C'CwwG_G
Cuts at: 251 632 1310 1487 2466 2770 3124 251
Size: 381 678 177 979 304 354 2763
Fragments arranged by size:
2763 979 678 381 354 304 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 205 964 1864 1956 3219 3352 3811 4020 205
Size: 759 900 92 1263 133 459 209 1821
Fragments arranged by size:
1821 1263 900 759 459 209 133 92
TatI w'GTAC_w
Cuts at: 1544 1637 5151 1544
Size: 93 3514 2029
Fragments arranged by size:
3514 2029 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1043 1075 5153 5185 1043
Size: 32 4078 32 1494
Fragments arranged by size:
4078 1494 32 32
Tth111I GACn'n_nGTC
Cuts at: 1888 3433 1888
Size: 1545 4091
XbaI T'CTAG_A
Cuts at: 2667 2667
Size: 5636
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 363 1020 1146 1683 2316 3131 363
Size: 657 126 537 633 815 2868
Fragments arranged by size:
2868 815 657 633 537 126
XmaI C'CCGG_G
Cuts at: 968 2656 968
Size: 1688 3948
ZraI GAC'GTC
Cuts at: 710 1424 1875 710
Size: 714 451 4471
Fragments arranged by size:
4471 714 451
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NruI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AgeI AloI AscI AvrII BaeI BfrBI BmgBI
BmtI BsiWI BsmBI BspEI BsrGI BssHII Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NsiI PacI PshAI PsiI PspXI PsrI SnaBI
SrfI SspI SwaI XhoI XmnI