Restriction map for
pF1KB8728
(Circular) (Six-base) MAPSORT of: pF1KB8728.seq Check: 6596 from: 1 to: 4229
pF1KB8728 4229 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 610 1070 1267 610
Size: 460 197 3572
Fragments arranged by size:
3572 460 197
Acc65I G'GTAC_C
Cuts at: 1245 1245
Size: 4229
AclI AA'CG_TT
Cuts at: 4069 4069
Size: 4229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 2054 2486 2839 3501 745
Size: 1309 432 353 662 1473
Fragments arranged by size:
1473 1309 662 432 353
AflIII A'CryG_T
Cuts at: 1581 3381 3662 1581
Size: 1800 281 2148
Fragments arranged by size:
2148 1800 281
AleI CACnn'nnGTG
Cuts at: 577 577
Size: 4229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1205 1205
Size: 4229
AlwNI CAG_nnn'CTG
Cuts at: 248 606 861 1534 2972 248
Size: 358 255 673 1438 1505
Fragments arranged by size:
1505 1438 673 358 255
ApaLI G'TGCA_C
Cuts at: 580 3067 580
Size: 2487 1742
ApoI r'AATT_y
Cuts at: 894 1233 3582 3838 894
Size: 339 2349 256 1285
Fragments arranged by size:
2349 1285 339 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4229
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
AvaI C'yCGr_G
Cuts at: 1249 1249
Size: 4229
BamHI G'GATC_C
Cuts at: 1254 1254
Size: 4229
BanI G'GyrC_C
Cuts at: 369 1245 1659 1907 1942 369
Size: 876 414 248 35 2656
Fragments arranged by size:
2656 876 414 248 35
BanII G_rGCy'C
Cuts at: 128 1243 2273 128
Size: 1115 1030 2084
Fragments arranged by size:
2084 1115 1030
BbeI G_GCGC'C
Cuts at: 1663 1911 1663
Size: 248 3981
BbsI GAAGACnn'nnnn_
Cuts at: 209 733 820 1148 209
Size: 524 87 328 3290
Fragments arranged by size:
3290 524 328 87
BbvCI CC'TCA_GC
Cuts at: 989 989
Size: 4229
BciVI GTATCCnnnnn_n'
Cuts at: 2127 3183 4229 2127
Size: 1056 1046 2127
Fragments arranged by size:
2127 1056 1046
BclI T'GATC_A
Cuts at: 1749 1749
Size: 4229
BglII A'GATC_T
Cuts at: 180 911 1744 3781 180
Size: 731 833 2037 628
Fragments arranged by size:
2037 833 731 628
BlpI GC'TnA_GC
Cuts at: 1341 1341
Size: 4229
Bme1580I G_kGCm'C
Cuts at: 374 506 584 1854 1947 3071 374
Size: 132 78 1270 93 1124 1532
Fragments arranged by size:
1532 1270 1124 132 93 78
BmrI ACTGGGnnnn_n'
Cuts at: 1441 1522 1857 4001 1441
Size: 81 335 2144 1669
Fragments arranged by size:
2144 1669 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 4229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 490 754 1022 448
Size: 42 264 268 3655
Fragments arranged by size:
3655 268 264 42
Bpu10I CC'TnA_GC
Cuts at: 989 989
Size: 4229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1405 2284 2998 3296 1405
Size: 879 714 298 2338
Fragments arranged by size:
2338 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3933 3933
Size: 4229
BsaAI yAC'GTr
Cuts at: 1038 1582 1038
Size: 544 3685
BsaBI GATnn'nnATC
Cuts at: 6 1743 3780 6
Size: 1737 2037 455
Fragments arranged by size:
2037 1737 455
BsaHI Gr'CG_yC
Cuts at: 740 1660 1908 2610 4106 740
Size: 920 248 702 1496 863
Fragments arranged by size:
1496 920 863 702 248
BsaWI w'CCGG_w
Cuts at: 1054 1076 1418 1939 2617 3028 3175 3449 1054
Size: 22 342 521 678 411 147 274 1834
Fragments arranged by size:
1834 678 521 411 342 274 147 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 408 1006 408
Size: 598 3631
BseYI C'CCAG_C
Cuts at: 1654 2412 3077 3485 1654
Size: 758 665 408 2398
Fragments arranged by size:
2398 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 730
Size: 4229
BsiEI CG_ry'CG
Cuts at: 104 1771 1817 3047 104
Size: 1667 46 1230 1286
Fragments arranged by size:
1667 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 584 1243 2021 2211 3071 584
Size: 659 778 190 860 1742
Fragments arranged by size:
1742 860 778 659 190
BsmI GAATG_Cn'
Cuts at: 1086 1086
Size: 4229
Bsp1286I G_dGCh'C
Cuts at: 128 374 506 584 1243 1854 1947 2021 2211
Size: 246 132 78 659 611 93 74 190
Cuts at: 2211 2273 3071 128
Size: 62 798 1286
Fragments arranged by size:
1286 798 659 611 246 190 132 93
78 74 62
BspEI T'CCGG_A
Cuts at: 1076 1076
Size: 4229
BspHI T'CATG_A
Cuts at: 2661 2661
Size: 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 304 1279 1493 1795 2176 304
Size: 975 214 302 381 2357
Fragments arranged by size:
2357 975 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2521 2575 4059 4224 50
Size: 2471 54 1484 165 55
Fragments arranged by size:
2471 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2141 2141
Size: 4229
BsrFI r'CCGG_y
Cuts at: 446 2227 2408 446
Size: 1781 181 2267
Fragments arranged by size:
2267 1781 181
BssSI C'ACGA_G
Cuts at: 2500 3208 2500
Size: 708 3521
BstAPI GCAn_nnn'nTGC
Cuts at: 1606 1606
Size: 4229
BstBI TT'CG_AA
Cuts at: 2590 2590
Size: 4229
BstYI r'GATC_y
Cuts at: 1 180 911 956 1254 1721 1744 2079 2325
Size: 179 731 45 298 467 23 335 246
Cuts at: 2325 2631 2643 2729 3781 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 731 467 449 335 306 298 246
179 86 45 23 12
BstZ17I GTA'TAC
Cuts at: 611 611
Size: 4229
Bsu36I CC'TnA_GG
Cuts at: 130 130
Size: 4229
BtgI C'CryG_G
Cuts at: 653 819 653
Size: 166 4063
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2179 2360 2523 2179
Size: 181 163 3885
Fragments arranged by size:
3885 181 163
BtsI GCAGTG_nn'
Cuts at: 464 747 1472 464
Size: 283 725 3221
Fragments arranged by size:
3221 725 283
DraI TTT'AAA
Cuts at: 1228 1228
Size: 4229
DraIII CAC_nnn'GTG
Cuts at: 588 588
Size: 4229
DrdI GACnn_nn'nnGTC
Cuts at: 1935 3279 1935
Size: 1344 2885
EaeI y'GGCC_r
Cuts at: 1814 1988 2379 2406 3751 1814
Size: 174 391 27 1345 2292
Fragments arranged by size:
2292 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1814 1814
Size: 4229
EarI CTCTTCn'nnn_
Cuts at: 211 352 2252 2462 211
Size: 141 1900 210 1978
Fragments arranged by size:
1978 1900 210 141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2437 3179 3325 4039 2437
Size: 742 146 714 2627
Fragments arranged by size:
2627 742 714 146
EcoICRI GAG'CTC
Cuts at: 1241 1241
Size: 4229
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 490 745 754 1022 2054 2486 2839 3501
Size: 42 255 9 268 1032 432 353 662
Cuts at: 3501 448
Size: 1176
Fragments arranged by size:
1176 1032 662 432 353 268 255 42
9
EcoNI CCTnn'n_nnAGG
Cuts at: 559 559
Size: 4229
EcoO109I rG'GnC_Cy
Cuts at: 245 603 1368 245
Size: 358 765 3106
Fragments arranged by size:
3106 765 358
EcoRI G'AATT_C
Cuts at: 894 1233 894
Size: 339 3890
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 422 648 2248 422
Size: 226 1600 2403
Fragments arranged by size:
2403 1600 226
FspI TGC'GCA
Cuts at: 1034 2010 1034
Size: 976 3253
HaeII r_GCGC'y
Cuts at: 1663 1911 3141 3912 1663
Size: 248 1230 771 1980
Fragments arranged by size:
1980 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 697 729 872 904 982 1014 2071 2101 2103
Size: 32 143 32 78 32 1057 30 2
Cuts at: 2103 2133 697
Size: 30 2793
Fragments arranged by size:
2793 1057 143 78 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 645 1268 645
Size: 623 3606
KasI G'GCGC_C
Cuts at: 1659 1907 1659
Size: 248 3981
KpnI G_GTAC'C
Cuts at: 1249 1249
Size: 4229
MluI A'CGCG_T
Cuts at: 3662 3662
Size: 4229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 540 2988 3172 540
Size: 2448 184 1597
Fragments arranged by size:
2448 1597 184
MscI TGG'CCA
Cuts at: 1990 1990
Size: 4229
MslI CAynn'nnrTG
Cuts at: 577 1064 1213 2218 2345 577
Size: 487 149 1005 127 2461
Fragments arranged by size:
2461 1005 487 149 127
MspA1I CmG'CkG
Cuts at: 1340 1654 2014 2798 3043 1340
Size: 314 360 784 245 2526
Fragments arranged by size:
2526 784 360 314 245
NaeI GCC'GGC
Cuts at: 448 2410 448
Size: 1962 2267
NarI GG'CG_CC
Cuts at: 1660 1908 1660
Size: 248 3981
NcoI C'CATG_G
Cuts at: 653 653
Size: 4229
NgoMIV G'CCGG_C
Cuts at: 446 2408 446
Size: 1962 2267
NspI r_CATG'y
Cuts at: 681 1282 1782 3385 681
Size: 601 500 1603 1525
Fragments arranged by size:
1603 1525 601 500
PasI CC'CwG_GG
Cuts at: 242 374 764 242
Size: 132 390 3707
Fragments arranged by size:
3707 390 132
PciI A'CATG_T
Cuts at: 3381 3381
Size: 4229
PflMI CCAn_nnn'nTGG
Cuts at: 248 942 248
Size: 694 3535
PmeI GTTT'AAAC
Cuts at: 1228 1228
Size: 4229
PmlI CAC'GTG
Cuts at: 1038 1038
Size: 4229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2675 2707 2675
Size: 32 4197
PshAI GACnn'nnGTC
Cuts at: 1055 1055
Size: 4229
PstI C_TGCA'G
Cuts at: 491 602 1276 1961 491
Size: 111 674 685 2759
Fragments arranged by size:
2759 685 674 111
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4229
PvuII CAG'CTG
Cuts at: 1654 2014 1654
Size: 360 3869
RsrII CG'GwC_CG
Cuts at: 2424 2424
Size: 4229
SacI G_AGCT'C
Cuts at: 1243 1243
Size: 4229
SalI G'TCGA_C
Cuts at: 1266 1266
Size: 4229
SapI GCTCTTCn'nnn_
Cuts at: 2252 2462 2252
Size: 210 4019
SbfI CC_TGCA'GG
Cuts at: 1276 1276
Size: 4229
ScaI AGT'ACT
Cuts at: 3746 3746
Size: 4229
SfcI C'TryA_G
Cuts at: 33 487 598 1272 1957 2925 3116 33
Size: 454 111 674 685 968 191 1146
Fragments arranged by size:
1146 968 685 674 454 191 111
SfoI GGC'GCC
Cuts at: 1661 1909 1661
Size: 248 3981
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
SmaI CCC'GGG
Cuts at: 1251 1251
Size: 4229
SmlI C'TyrA_G
Cuts at: 1384 2299 3013 3275 1384
Size: 915 714 262 2338
Fragments arranged by size:
2338 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4229
SphI G_CATG'C
Cuts at: 681 1282 1782 681
Size: 601 500 3128
Fragments arranged by size:
3128 601 500
SspI AAT'ATT
Cuts at: 1122 1122
Size: 4229
StyI C'CwwG_G
Cuts at: 653 1363 1717 653
Size: 710 354 3165
Fragments arranged by size:
3165 710 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1812 1945 2404 2613 1812
Size: 133 459 209 3428
Fragments arranged by size:
3428 459 209 133
TatI w'GTAC_w
Cuts at: 926 3744 926
Size: 2818 1411
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3746 3778 3746
Size: 32 4197
Tth111I GACn'n_nGTC
Cuts at: 2026 2026
Size: 4229
XbaI T'CTAG_A
Cuts at: 1260 1260
Size: 4229
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1125 1724 1125
Size: 599 3630
XmaI C'CCGG_G
Cuts at: 1249 1249
Size: 4229
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstXI ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PfoI PpuMI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI