Restriction map for
pF1KB8725
(Circular) (Six-base) MAPSORT of: pF1KB8725.seq Check: 1335 from: 1 to: 5105
pF1KB8725 5105 bp
With 182 enzymes: SgfI *
July 15, 2008 09:38 ..
AccI GT'mk_AC
Cuts at: 536 2143 536
Size: 1607 3498
Acc65I G'GTAC_C
Cuts at: 1174 2121 1174
Size: 947 4158
AclI AA'CG_TT
Cuts at: 4945 4945
Size: 5105
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 371 1136 1896 2930 3362 3715 4377 371
Size: 765 760 1034 432 353 662 1099
Fragments arranged by size:
1099 1034 765 760 662 432 353
AfeI AGC'GCT
Cuts at: 1006 1006
Size: 5105
AflIII A'CryG_T
Cuts at: 2457 4257 4538 2457
Size: 1800 281 3024
Fragments arranged by size:
3024 1800 281
AleI CACnn'nnGTG
Cuts at: 1729 1729
Size: 5105
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1970 1970
Size: 5105
AlwNI CAG_nnn'CTG
Cuts at: 1655 2410 3848 1655
Size: 755 1438 2912
Fragments arranged by size:
2912 1438 755
ApaLI G'TGCA_C
Cuts at: 981 1678 1955 3943 981
Size: 697 277 1988 2143
Fragments arranged by size:
2143 1988 697 277
ApoI r'AATT_y
Cuts at: 2109 4458 4714 2109
Size: 2349 256 2500
Fragments arranged by size:
2500 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5105
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5105
AvaI C'yCGr_G
Cuts at: 597 2125 597
Size: 1528 3577
AvrII C'CTAG_G
Cuts at: 1578 1578
Size: 5105
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1166 1199 1166
Size: 33 5072
BamHI G'GATC_C
Cuts at: 527 2130 527
Size: 1603 3502
BanI G'GyrC_C
Cuts at: 1174 1428 2121 2535 2783 2818 1174
Size: 254 693 414 248 35 3461
Fragments arranged by size:
3461 693 414 254 248 35
BanII G_rGCy'C
Cuts at: 130 173 1226 1285 1373 1649 2119 3149 130
Size: 43 1053 59 88 276 470 1030 2086
Fragments arranged by size:
2086 1053 1030 470 276 88 59 43
BbeI G_GCGC'C
Cuts at: 2539 2787 2539
Size: 248 4857
BbsI GAAGACnn'nnnn_
Cuts at: 1509 1509
Size: 5105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1401 1435 1401
Size: 34 5071
BciVI GTATCCnnnnn_n'
Cuts at: 3003 4059 5105 3003
Size: 1056 1046 3003
Fragments arranged by size:
3003 1056 1046
BclI T'GATC_A
Cuts at: 734 1106 2625 734
Size: 372 1519 3214
Fragments arranged by size:
3214 1519 372
BfrBI ATG'CAT
Cuts at: 2089 2089
Size: 5105
BglII A'GATC_T
Cuts at: 578 2620 4657 578
Size: 2042 2037 1026
Fragments arranged by size:
2042 2037 1026
BlpI GC'TnA_GC
Cuts at: 2217 2217
Size: 5105
Bme1580I G_kGCm'C
Cuts at: 985 1433 1682 1959 2730 2823 3947 985
Size: 448 249 277 771 93 1124 2143
Fragments arranged by size:
2143 1124 771 448 277 249 93
BmrI ACTGGGnnnn_n'
Cuts at: 386 773 2317 2398 2733 4877 386
Size: 387 1544 81 335 2144 614
Fragments arranged by size:
2144 1544 614 387 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 343 931 3472 343
Size: 588 2541 1976
Fragments arranged by size:
2541 1976 588
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 144
Size: 5105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2281 3160 3874 4172 2281
Size: 879 714 298 3214
Fragments arranged by size:
3214 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 154 917 1858 4809 154
Size: 763 941 2951 450
Fragments arranged by size:
2951 941 763 450
BsaAI yAC'GTr
Cuts at: 1035 1603 2458 1035
Size: 568 855 3682
Fragments arranged by size:
3682 855 568
BsaBI GATnn'nnATC
Cuts at: 6 958 1411 1769 2619 4656 6
Size: 952 453 358 850 2037 455
Fragments arranged by size:
2037 952 850 455 453 358
BsaHI Gr'CG_yC
Cuts at: 797 2536 2784 3486 4982 797
Size: 1739 248 702 1496 920
Fragments arranged by size:
1739 1496 920 702 248
BsaWI w'CCGG_w
Cuts at: 1761 2294 2815 3493 3904 4051 4325 1761
Size: 533 521 678 411 147 274 2541
Fragments arranged by size:
2541 678 533 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1735 1825 1852 1993 1735
Size: 90 27 141 4847
Fragments arranged by size:
4847 141 90 27
BseYI C'CCAG_C
Cuts at: 441 475 1648 2530 3288 3953 4361 441
Size: 34 1173 882 758 665 408 1185
Fragments arranged by size:
1185 1173 882 758 665 408 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1021 1459 1021
Size: 438 4667
BsiEI CG_ry'CG
Cuts at: 104 2647 2693 3923 104
Size: 2543 46 1230 1286
Fragments arranged by size:
2543 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 173 985 1226 1285 1373 1682 1959 2119 2897
Size: 812 241 59 88 309 277 160 778
Cuts at: 2897 3087 3947 173
Size: 190 860 1331
Fragments arranged by size:
1331 860 812 778 309 277 241 190
160 88 59
BsmI GAATG_Cn'
Cuts at: 423 1115 423
Size: 692 4413
Bsp1286I G_dGCh'C
Cuts at: 130 173 985 1226 1285 1373 1433 1649 1682
Size: 43 812 241 59 88 60 216 33
Cuts at: 1682 1959 2119 2730 2823 2897 3087 3149 3947
Size: 277 160 611 93 74 190 62 798
Cuts at: 3947 130
Size: 1288
Fragments arranged by size:
1288 812 798 611 277 241 216 190
160 93 88 74 62 60 59 43
33
BspEI T'CCGG_A
Cuts at: 1761 1761
Size: 5105
BspHI T'CATG_A
Cuts at: 1277 1373 3537 1277
Size: 96 2164 2845
Fragments arranged by size:
2845 2164 96
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2155 2369 2671 3052 2155
Size: 214 302 381 4208
Fragments arranged by size:
4208 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3397 3451 4935 5100 50
Size: 3347 54 1484 165 55
Fragments arranged by size:
3347 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 186 1505 3017 186
Size: 1319 1512 2274
Fragments arranged by size:
2274 1512 1319
BsrFI r'CCGG_y
Cuts at: 3103 3284 3103
Size: 181 4924
BsrGI T'GTAC_A
Cuts at: 1121 1121
Size: 5105
BssSI C'ACGA_G
Cuts at: 166 3376 4084 166
Size: 3210 708 1187
Fragments arranged by size:
3210 1187 708
BstAPI GCAn_nnn'nTGC
Cuts at: 670 2095 2482 670
Size: 1425 387 3293
Fragments arranged by size:
3293 1425 387
BstBI TT'CG_AA
Cuts at: 3466 3466
Size: 5105
BstEII G'GTnAC_C
Cuts at: 144 2064 144
Size: 1920 3185
BstXI CCAn_nnnn'nTGG
Cuts at: 1494 1494
Size: 5105
BstYI r'GATC_y
Cuts at: 1 527 578 909 2130 2597 2620 2955 3201
Size: 526 51 331 1221 467 23 335 246
Cuts at: 3201 3507 3519 3605 4657 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1221 1052 526 467 449 335 331 306
246 86 51 23 12
Bsu36I CC'TnA_GG
Cuts at: 502 624 1859 502
Size: 122 1235 3748
Fragments arranged by size:
3748 1235 122
BtgI C'CryG_G
Cuts at: 107 140 931 1325 2069 107
Size: 33 791 394 744 3143
Fragments arranged by size:
3143 791 744 394 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 378 1182 1404 3055 3236 3399 378
Size: 804 222 1651 181 163 2084
Fragments arranged by size:
2084 1651 804 222 181 163
BtsI GCAGTG_nn'
Cuts at: 668 2348 668
Size: 1680 3425
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1196 1231 1196
Size: 35 5070
DraI TTT'AAA
Cuts at: 2104 2104
Size: 5105
DraIII CAC_nnn'GTG
Cuts at: 360 783 1453 360
Size: 423 670 4012
Fragments arranged by size:
4012 670 423
DrdI GACnn_nn'nnGTC
Cuts at: 2811 4155 2811
Size: 1344 3761
EaeI y'GGCC_r
Cuts at: 227 310 351 368 2690 2864 3255 3282 4627
Size: 83 41 17 2322 174 391 27 1345
Cuts at: 4627 227
Size: 705
Fragments arranged by size:
2322 1345 705 391 174 83 41 27
17
EagI C'GGCC_G
Cuts at: 2690 2690
Size: 5105
EarI CTCTTCn'nnn_
Cuts at: 1324 1468 1534 1711 1829 1970 3128 3338 1324
Size: 144 66 177 118 141 1158 210 3091
Fragments arranged by size:
3091 1158 210 177 144 141 118 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3313 4055 4201 4915 3313
Size: 742 146 714 3503
Fragments arranged by size:
3503 742 714 146
EcoICRI GAG'CTC
Cuts at: 171 1224 1283 1371 2117 171
Size: 1053 59 88 746 3159
Fragments arranged by size:
3159 1053 746 88 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 371 1136 1896 2930 3362 3715 4377 144
Size: 227 765 760 1034 432 353 662 872
Fragments arranged by size:
1034 872 765 760 662 432 353 227
EcoO109I rG'GnC_Cy
Cuts at: 693 1855 2244 693
Size: 1162 389 3554
Fragments arranged by size:
3554 1162 389
EcoRI G'AATT_C
Cuts at: 2109 2109
Size: 5105
EcoRV GAT'ATC
Cuts at: 192 192
Size: 5105
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3124 3124
Size: 5105
FspI TGC'GCA
Cuts at: 1798 2886 1798
Size: 1088 4017
HaeII r_GCGC'y
Cuts at: 1008 1310 2539 2787 4017 4788 1008
Size: 302 1229 248 1230 771 1325
Fragments arranged by size:
1325 1230 1229 771 302 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 580 612 2947 2977 2979 3009 580
Size: 32 2335 30 2 30 2676
Fragments arranged by size:
2676 2335 32 30 30 2
HincII GTy'rAC
Cuts at: 2144 2144
Size: 5105
KasI G'GCGC_C
Cuts at: 2535 2783 2535
Size: 248 4857
KpnI G_GTAC'C
Cuts at: 1178 2125 1178
Size: 947 4158
MluI A'CGCG_T
Cuts at: 4538 4538
Size: 5105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 213 252 853 1231 1768 3864 4048 213
Size: 39 601 378 537 2096 184 1270
Fragments arranged by size:
2096 1270 601 537 378 184 39
MscI TGG'CCA
Cuts at: 353 2866 353
Size: 2513 2592
MslI CAynn'nnrTG
Cuts at: 742 1090 1093 1729 1993 2062 3094 3221 742
Size: 348 3 636 264 69 1032 127 2626
Fragments arranged by size:
2626 1032 636 348 264 127 69 3
MspA1I CmG'CkG
Cuts at: 142 525 2216 2530 2890 3674 3919 142
Size: 383 1691 314 360 784 245 1328
Fragments arranged by size:
1691 1328 784 383 360 314 245
NaeI GCC'GGC
Cuts at: 3286 3286
Size: 5105
NarI GG'CG_CC
Cuts at: 2536 2784 2536
Size: 248 4857
NcoI C'CATG_G
Cuts at: 107 931 1325 107
Size: 824 394 3887
Fragments arranged by size:
3887 824 394
NgoMIV G'CCGG_C
Cuts at: 3284 3284
Size: 5105
NsiI A_TGCA'T
Cuts at: 2091 2091
Size: 5105
NspI r_CATG'y
Cuts at: 2158 2658 4261 2158
Size: 500 1603 3002
Fragments arranged by size:
3002 1603 500
PciI A'CATG_T
Cuts at: 4257 4257
Size: 5105
PflMI CCAn_nnn'nTGG
Cuts at: 360 937 360
Size: 577 4528
PmeI GTTT'AAAC
Cuts at: 2104 2104
Size: 5105
PmlI CAC'GTG
Cuts at: 1035 1035
Size: 5105
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3551 3583 3551
Size: 32 5073
PstI C_TGCA'G
Cuts at: 2152 2837 2152
Size: 685 4420
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5105
PvuII CAG'CTG
Cuts at: 2530 2890 2530
Size: 360 4745
RsrII CG'GwC_CG
Cuts at: 3300 3300
Size: 5105
SacI G_AGCT'C
Cuts at: 173 1226 1285 1373 2119 173
Size: 1053 59 88 746 3159
Fragments arranged by size:
3159 1053 746 88 59
SacII CC_GC'GG
Cuts at: 143 143
Size: 5105
SalI G'TCGA_C
Cuts at: 2142 2142
Size: 5105
SapI GCTCTTCn'nnn_
Cuts at: 3128 3338 3128
Size: 210 4895
SbfI CC_TGCA'GG
Cuts at: 2152 2152
Size: 5105
ScaI AGT'ACT
Cuts at: 4622 4622
Size: 5105
SfcI C'TryA_G
Cuts at: 33 2148 2833 3801 3992 33
Size: 2115 685 968 191 1146
Fragments arranged by size:
2115 1146 968 685 191
SfoI GGC'GCC
Cuts at: 2537 2785 2537
Size: 248 4857
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5105
SmaI CCC'GGG
Cuts at: 2127 2127
Size: 5105
SmlI C'TyrA_G
Cuts at: 2260 3175 3889 4151 2260
Size: 915 714 262 3214
Fragments arranged by size:
3214 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5105
SphI G_CATG'C
Cuts at: 2158 2658 2158
Size: 500 4605
SspI AAT'ATT
Cuts at: 1168 1168
Size: 5105
StuI AGG'CCT
Cuts at: 629 1247 629
Size: 618 4487
StyI C'CwwG_G
Cuts at: 107 880 931 1178 1325 1340 1578 2239 2593
Size: 773 51 247 147 15 238 661 354
Cuts at: 2593 107
Size: 2619
Fragments arranged by size:
2619 773 661 354 247 238 147 51
15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1416 1518 1974 2688 2821 3280 3489 1416
Size: 102 456 714 133 459 209 3032
Fragments arranged by size:
3032 714 459 456 209 133 102
TatI w'GTAC_w
Cuts at: 812 1121 1688 4620 812
Size: 309 567 2932 1297
Fragments arranged by size:
2932 1297 567 309
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4622 4654 4622
Size: 32 5073
Tth111I GACn'n_nGTC
Cuts at: 2902 2902
Size: 5105
XbaI T'CTAG_A
Cuts at: 2136 2136
Size: 5105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1086 1562 2600 1086
Size: 476 1038 3591
Fragments arranged by size:
3591 1038 476
XmaI C'CCGG_G
Cuts at: 2125 2125
Size: 5105
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PmeI PmlI
PpiI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AloI ApaI AscI
BbvCI BglI BmgBI BmtI Bpu10I BsaXI BsiWI BsmBI
BssHII BstZ17I ClaI EcoNI FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PasI PfoI
PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI