Restriction map for
pF1KB8723
(Circular) (Six-base) MAPSORT of: pF1KB8723.seq Check: 1709 from: 1 to: 4640
pF1KB8723 4640 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 352 352
Size: 4640
AccI GT'mk_AC
Cuts at: 644 897 1678 644
Size: 253 781 3606
Fragments arranged by size:
3606 781 253
Acc65I G'GTAC_C
Cuts at: 1465 1656 1465
Size: 191 4449
AclI AA'CG_TT
Cuts at: 1634 4480 1634
Size: 2846 1794
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 334 766 2465 2897 3250 3912 232
Size: 102 432 1699 432 353 662 960
Fragments arranged by size:
1699 960 662 432 432 353 102
AflII C'TTAA_G
Cuts at: 1338 1338
Size: 4640
AflIII A'CryG_T
Cuts at: 1992 3792 4073 1992
Size: 1800 281 2559
Fragments arranged by size:
2559 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 207 312 999 1254 1945 3383 207
Size: 105 687 255 691 1438 1464
Fragments arranged by size:
1464 1438 691 687 255 105
ApaLI G'TGCA_C
Cuts at: 3478 3478
Size: 4640
ApoI r'AATT_y
Cuts at: 859 1353 1644 3993 4249 859
Size: 494 291 2349 256 1250
Fragments arranged by size:
2349 1250 494 291 256
AseI AT'TA_AT
Cuts at: 20 887 20
Size: 867 3773
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
AvaI C'yCGr_G
Cuts at: 1204 1660 1204
Size: 456 4184
BamHI G'GATC_C
Cuts at: 1665 1665
Size: 4640
BanI G'GyrC_C
Cuts at: 664 1465 1656 2070 2318 2353 664
Size: 801 191 414 248 35 2951
Fragments arranged by size:
2951 801 414 248 191 35
BanII G_rGCy'C
Cuts at: 263 1211 1607 1654 2684 263
Size: 948 396 47 1030 2219
Fragments arranged by size:
2219 1030 948 396 47
BbeI G_GCGC'C
Cuts at: 2074 2322 2074
Size: 248 4392
BciVI GTATCCnnnnn_n'
Cuts at: 1439 2538 3594 4640 1439
Size: 1099 1056 1046 1439
Fragments arranged by size:
1439 1099 1056 1046
BclI T'GATC_A
Cuts at: 1284 2160 1284
Size: 876 3764
BfrBI ATG'CAT
Cuts at: 922 1128 922
Size: 206 4434
BglII A'GATC_T
Cuts at: 2155 4192 2155
Size: 2037 2603
BlpI GC'TnA_GC
Cuts at: 563 1405 1752 563
Size: 842 347 3451
Fragments arranged by size:
3451 842 347
Bme1580I G_kGCm'C
Cuts at: 1618 2265 2358 3482 1618
Size: 647 93 1124 2776
Fragments arranged by size:
2776 1124 647 93
BmrI ACTGGGnnnn_n'
Cuts at: 960 1852 1933 2268 4412 960
Size: 892 81 335 2144 1188
Fragments arranged by size:
2144 1188 892 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1251 3007 1251
Size: 1756 2884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 409 1231 1246 1462 1498 262
Size: 147 822 15 216 36 3404
Fragments arranged by size:
3404 822 216 147 36 15
Bpu10I CC'TnA_GC
Cuts at: 1057 1312 1507 1057
Size: 255 195 4190
Fragments arranged by size:
4190 255 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 1816 2695 3409 3707 640
Size: 1176 879 714 298 1573
Fragments arranged by size:
1573 1176 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 385 4344 385
Size: 3959 681
BsaAI yAC'GTr
Cuts at: 1065 1422 1993 1065
Size: 357 571 3712
Fragments arranged by size:
3712 571 357
BsaBI GATnn'nnATC
Cuts at: 6 2154 4191 6
Size: 2148 2037 455
Fragments arranged by size:
2148 2037 455
BsaHI Gr'CG_yC
Cuts at: 2071 2319 3021 4517 2071
Size: 248 702 1496 2194
Fragments arranged by size:
2194 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1829 2350 3028 3439 3586 3860 1829
Size: 521 678 411 147 274 2609
Fragments arranged by size:
2609 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1504 1534 1504
Size: 30 4610
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 892 895 892
Size: 3 4637
BseYI C'CCAG_C
Cuts at: 1144 1325 1537 2065 2823 3488 3896 1144
Size: 181 212 528 758 665 408 1888
Fragments arranged by size:
1888 758 665 528 408 212 181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 817 346
Size: 471 4169
BsiEI CG_ry'CG
Cuts at: 104 2182 2228 3458 104
Size: 2078 46 1230 1286
Fragments arranged by size:
2078 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 263 428 604 641 1654 2432 2622 3482 263
Size: 165 176 37 1013 778 190 860 1421
Fragments arranged by size:
1421 1013 860 778 190 176 165 37
Bsp1286I G_dGCh'C
Cuts at: 263 428 604 641 1211 1607 1618 1654 2265
Size: 165 176 37 570 396 11 36 611
Cuts at: 2265 2358 2432 2622 2684 3482 263
Size: 93 74 190 62 798 1421
Fragments arranged by size:
1421 798 611 570 396 190 176 165
93 74 62 37 36 11
BspHI T'CATG_A
Cuts at: 3072 3072
Size: 4640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 352 1690 1904 2206 2587 352
Size: 1338 214 302 381 2405
Fragments arranged by size:
2405 1338 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2932 2986 4470 4635 50
Size: 2882 54 1484 165 55
Fragments arranged by size:
2882 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 230 326 764 2552 230
Size: 96 438 1788 2318
Fragments arranged by size:
2318 1788 438 96
BsrFI r'CCGG_y
Cuts at: 1103 2638 2819 1103
Size: 1535 181 2924
Fragments arranged by size:
2924 1535 181
BssSI C'ACGA_G
Cuts at: 2911 3619 2911
Size: 708 3932
BstAPI GCAn_nnn'nTGC
Cuts at: 207 999 2017 207
Size: 792 1018 2830
Fragments arranged by size:
2830 1018 792
BstBI TT'CG_AA
Cuts at: 3001 3001
Size: 4640
BstYI r'GATC_y
Cuts at: 1 1665 2132 2155 2490 2736 3042 3054 3140
Size: 1664 467 23 335 246 306 12 86
Cuts at: 3140 4192 1
Size: 1052 449
Fragments arranged by size:
1664 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 175 1461 107
Size: 68 1286 3286
Fragments arranged by size:
3286 1286 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2590 2771 2934 2590
Size: 181 163 4296
Fragments arranged by size:
4296 181 163
BtsI GCAGTG_nn'
Cuts at: 1000 1317 1883 1000
Size: 317 566 3757
Fragments arranged by size:
3757 566 317
DraI TTT'AAA
Cuts at: 1639 1639
Size: 4640
DrdI GACnn_nn'nnGTC
Cuts at: 513 2346 3690 513
Size: 1833 1344 1463
Fragments arranged by size:
1833 1463 1344
EaeI y'GGCC_r
Cuts at: 227 2225 2399 2790 2817 4162 227
Size: 1998 174 391 27 1345 705
Fragments arranged by size:
1998 1345 705 391 174 27
EagI C'GGCC_G
Cuts at: 2225 2225
Size: 4640
EarI CTCTTCn'nnn_
Cuts at: 268 280 944 2663 2873 268
Size: 12 664 1719 210 2035
Fragments arranged by size:
2035 1719 664 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2848 3590 3736 4450 2848
Size: 742 146 714 3038
Fragments arranged by size:
3038 742 714 146
EcoICRI GAG'CTC
Cuts at: 261 1652 261
Size: 1391 3249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 262 334 409 766 1231 1246 1462 1498
Size: 30 72 75 357 465 15 216 36
Cuts at: 1498 2465 2897 3250 3912 232
Size: 967 432 353 662 960
Fragments arranged by size:
967 960 662 465 432 357 353 216
75 72 36 30 15
EcoNI CCTnn'n_nnAGG
Cuts at: 211 433 211
Size: 222 4418
EcoO109I rG'GnC_Cy
Cuts at: 222 1251 1779 222
Size: 1029 528 3083
Fragments arranged by size:
3083 1029 528
EcoRI G'AATT_C
Cuts at: 1353 1644 1353
Size: 291 4349
EcoRV GAT'ATC
Cuts at: 774 774
Size: 4640
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 424 2659 424
Size: 2235 2405
FspI TGC'GCA
Cuts at: 2421 2421
Size: 4640
HaeII r_GCGC'y
Cuts at: 2074 2322 3552 4323 2074
Size: 248 1230 771 2391
Fragments arranged by size:
2391 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 873 890 905 922 2482 2512 2514 2544 873
Size: 17 15 17 1560 30 2 30 2969
Fragments arranged by size:
2969 1560 30 30 17 17 15 2
HincII GTy'rAC
Cuts at: 1679 1679
Size: 4640
HindIII A'AGCT_T
Cuts at: 1341 1341
Size: 4640
KasI G'GCGC_C
Cuts at: 2070 2318 2070
Size: 248 4392
KpnI G_GTAC'C
Cuts at: 1469 1660 1469
Size: 191 4449
MluI A'CGCG_T
Cuts at: 4073 4073
Size: 4640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3399 3583 3399
Size: 184 4456
MscI TGG'CCA
Cuts at: 229 2401 229
Size: 2172 2468
MslI CAynn'nnrTG
Cuts at: 154 160 917 2629 2756 154
Size: 6 757 1712 127 2038
Fragments arranged by size:
2038 1712 757 127 6
MspA1I CmG'CkG
Cuts at: 801 1751 2065 2425 3209 3454 801
Size: 950 314 360 784 245 1987
Fragments arranged by size:
1987 950 784 360 314 245
NaeI GCC'GGC
Cuts at: 1105 2821 1105
Size: 1716 2924
NarI GG'CG_CC
Cuts at: 2071 2319 2071
Size: 248 4392
NcoI C'CATG_G
Cuts at: 107 1461 107
Size: 1354 3286
NgoMIV G'CCGG_C
Cuts at: 1103 2819 1103
Size: 1716 2924
NsiI A_TGCA'T
Cuts at: 924 1130 924
Size: 206 4434
NspI r_CATG'y
Cuts at: 1501 1693 2193 3796 1501
Size: 192 500 1603 2345
Fragments arranged by size:
2345 1603 500 192
PasI CC'CwG_GG
Cuts at: 254 254
Size: 4640
PciI A'CATG_T
Cuts at: 3792 3792
Size: 4640
PflMI CCAn_nnn'nTGG
Cuts at: 455 1254 455
Size: 799 3841
PfoI T'CCnGG_A
Cuts at: 1247 1247
Size: 4640
PmeI GTTT'AAAC
Cuts at: 1639 1639
Size: 4640
PmlI CAC'GTG
Cuts at: 1065 1422 1065
Size: 357 4283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3086 3118 3086
Size: 32 4608
PpuMI rG'GwC_Cy
Cuts at: 1251 1251
Size: 4640
PstI C_TGCA'G
Cuts at: 995 1004 1391 1687 2372 995
Size: 9 387 296 685 3263
Fragments arranged by size:
3263 685 387 296 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4640
PvuII CAG'CTG
Cuts at: 801 2065 2425 801
Size: 1264 360 3016
Fragments arranged by size:
3016 1264 360
RsrII CG'GwC_CG
Cuts at: 2835 2835
Size: 4640
SacI G_AGCT'C
Cuts at: 263 1654 263
Size: 1391 3249
SalI G'TCGA_C
Cuts at: 1677 1677
Size: 4640
SapI GCTCTTCn'nnn_
Cuts at: 268 944 2663 2873 268
Size: 676 1719 210 2035
Fragments arranged by size:
2035 1719 676 210
SbfI CC_TGCA'GG
Cuts at: 1004 1687 1004
Size: 683 3957
ScaI AGT'ACT
Cuts at: 4157 4157
Size: 4640
SexAI A'CCwGG_T
Cuts at: 1253 1253
Size: 4640
SfcI C'TryA_G
Cuts at: 33 991 1000 1387 1683 2368 3336 3527 33
Size: 958 9 387 296 685 968 191 1146
Fragments arranged by size:
1146 968 958 685 387 296 191 9
SfoI GGC'GCC
Cuts at: 2072 2320 2072
Size: 248 4392
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
SmaI CCC'GGG
Cuts at: 1662 1662
Size: 4640
SmlI C'TyrA_G
Cuts at: 655 1338 1795 2710 3424 3686 655
Size: 683 457 915 714 262 1609
Fragments arranged by size:
1609 915 714 683 457 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4640
SphI G_CATG'C
Cuts at: 1501 1693 2193 1501
Size: 192 500 3948
Fragments arranged by size:
3948 500 192
StuI AGG'CCT
Cuts at: 331 331
Size: 4640
StyI C'CwwG_G
Cuts at: 107 851 1076 1461 1774 2128 107
Size: 744 225 385 313 354 2619
Fragments arranged by size:
2619 744 385 354 313 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1207 2223 2356 2815 3024 1207
Size: 1016 133 459 209 2823
Fragments arranged by size:
2823 1016 459 209 133
TatI w'GTAC_w
Cuts at: 536 720 4155 536
Size: 184 3435 1021
Fragments arranged by size:
3435 1021 184
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 407 439 1504 1536 4157 4189 407
Size: 32 1065 32 2621 32 858
Fragments arranged by size:
2621 1065 858 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1255 2437 1255
Size: 1182 3458
XbaI T'CTAG_A
Cuts at: 1671 1671
Size: 4640
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 237 2135 237
Size: 1898 2742
XmaI C'CCGG_G
Cuts at: 1660 1660
Size: 4640
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI