Restriction map for
pF1KB8722
(Circular) (Six-base) MAPSORT of: pF1KB8722.seq Check: 2224 from: 1 to: 4673
pF1KB8722 4673 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 257 482 1711 257
Size: 225 1229 3219
Fragments arranged by size:
3219 1229 225
Acc65I G'GTAC_C
Cuts at: 1689 1689
Size: 4673
AclI AA'CG_TT
Cuts at: 4513 4513
Size: 4673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1641 2498 2930 3283 3945 1641
Size: 857 432 353 662 2369
Fragments arranged by size:
2369 857 662 432 353
AfeI AGC'GCT
Cuts at: 849 849
Size: 4673
AflII C'TTAA_G
Cuts at: 316 316
Size: 4673
AflIII A'CryG_T
Cuts at: 2025 3825 4106 2025
Size: 1800 281 2592
Fragments arranged by size:
2592 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 363 363
Size: 4673
AleI CACnn'nnGTG
Cuts at: 1402 1402
Size: 4673
AlwNI CAG_nnn'CTG
Cuts at: 1664 1978 3416 1664
Size: 314 1438 2921
Fragments arranged by size:
2921 1438 314
ApaI G_GGCC'C
Cuts at: 1226 1525 1226
Size: 299 4374
ApaLI G'TGCA_C
Cuts at: 3511 3511
Size: 4673
ApoI r'AATT_y
Cuts at: 204 1677 4026 4282 204
Size: 1473 2349 256 595
Fragments arranged by size:
2349 1473 595 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4673
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
AvaI C'yCGr_G
Cuts at: 719 1693 719
Size: 974 3699
BamHI G'GATC_C
Cuts at: 1698 1698
Size: 4673
BanI G'GyrC_C
Cuts at: 1060 1689 2103 2351 2386 1060
Size: 629 414 248 35 3347
Fragments arranged by size:
3347 629 414 248 35
BanII G_rGCy'C
Cuts at: 554 740 1226 1525 1598 1687 2717 554
Size: 186 486 299 73 89 1030 2510
Fragments arranged by size:
2510 1030 486 299 186 89 73
BbeI G_GCGC'C
Cuts at: 2107 2355 2107
Size: 248 4425
BbsI GAAGACnn'nnnn_
Cuts at: 1462 1462
Size: 4673
BbvCI CC'TCA_GC
Cuts at: 1204 1204
Size: 4673
BciVI GTATCCnnnnn_n'
Cuts at: 2571 3627 4673 2571
Size: 1056 1046 2571
Fragments arranged by size:
2571 1056 1046
BclI T'GATC_A
Cuts at: 371 2193 371
Size: 1822 2851
BglI GCCn_nnn'nGGC
Cuts at: 493 493
Size: 4673
BglII A'GATC_T
Cuts at: 189 2188 4225 189
Size: 1999 2037 637
Fragments arranged by size:
2037 1999 637
BlpI GC'TnA_GC
Cuts at: 1785 1785
Size: 4673
Bme1580I G_kGCm'C
Cuts at: 1226 1525 2298 2391 3515 1226
Size: 299 773 93 1124 2384
Fragments arranged by size:
2384 1124 773 299 93
BmrI ACTGGGnnnn_n'
Cuts at: 256 412 1152 1631 1885 1966 2301 4445 256
Size: 156 740 479 254 81 335 2144 484
Fragments arranged by size:
2144 740 484 479 335 254 156 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 573 3040 573
Size: 2467 2206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 1006 565
Size: 441 4232
Bpu10I CC'TnA_GC
Cuts at: 1204 1204
Size: 4673
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1450 1849 2728 3442 3740 1450
Size: 399 879 714 298 2383
Fragments arranged by size:
2383 879 714 399 298
BsaI GGTCTCn'nnnn_
Cuts at: 709 718 874 1244 1474 4377 709
Size: 9 156 370 230 2903 1005
Fragments arranged by size:
2903 1005 370 230 156 9
BsaAI yAC'GTr
Cuts at: 951 2026 951
Size: 1075 3598
BsaBI GATnn'nnATC
Cuts at: 6 2187 4224 6
Size: 2181 2037 455
Fragments arranged by size:
2181 2037 455
BsaHI Gr'CG_yC
Cuts at: 2104 2352 3054 4550 2104
Size: 248 702 1496 2227
Fragments arranged by size:
2227 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1082 1862 2383 3061 3472 3619 3893 1082
Size: 780 521 678 411 147 274 1862
Fragments arranged by size:
1862 780 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1645 1675 1645
Size: 30 4643
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1393 1438 1393
Size: 45 4628
BseYI C'CCAG_C
Cuts at: 337 1563 2098 2856 3521 3929 337
Size: 1226 535 758 665 408 1081
Fragments arranged by size:
1226 1081 758 665 535 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 943 976 252
Size: 691 33 3949
Fragments arranged by size:
3949 691 33
BsiEI CG_ry'CG
Cuts at: 104 473 2215 2261 3491 104
Size: 369 1742 46 1230 1286
Fragments arranged by size:
1742 1286 1230 369 46
BsiHKAI G_wGCw'C
Cuts at: 740 1687 2465 2655 3515 740
Size: 947 778 190 860 1898
Fragments arranged by size:
1898 947 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 554 740 1226 1525 1598 1687 2298 2391 2465
Size: 186 486 299 73 89 611 93 74
Cuts at: 2465 2655 2717 3515 554
Size: 190 62 798 1712
Fragments arranged by size:
1712 798 611 486 299 190 186 93
89 74 73 62
BspEI T'CCGG_A
Cuts at: 1082 1082
Size: 4673
BspHI T'CATG_A
Cuts at: 3105 3105
Size: 4673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 545 1024 1723 1937 2239 2620 545
Size: 479 699 214 302 381 2598
Fragments arranged by size:
2598 699 479 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1452 2965 3019 4503 4668 50
Size: 1402 1513 54 1484 165 55
Fragments arranged by size:
1513 1484 1402 165 55 54
BsrDI GCAATG_nn'
Cuts at: 348 2585 348
Size: 2237 2436
BsrFI r'CCGG_y
Cuts at: 472 2671 2852 472
Size: 2199 181 2293
Fragments arranged by size:
2293 2199 181
BsrGI T'GTAC_A
Cuts at: 140 140
Size: 4673
BssSI C'ACGA_G
Cuts at: 2944 3652 2944
Size: 708 3965
BstAPI GCAn_nnn'nTGC
Cuts at: 2050 2050
Size: 4673
BstBI TT'CG_AA
Cuts at: 3034 3034
Size: 4673
BstXI CCAn_nnnn'nTGG
Cuts at: 597 870 597
Size: 273 4400
BstYI r'GATC_y
Cuts at: 1 189 1075 1382 1698 2165 2188 2523 2769
Size: 188 886 307 316 467 23 335 246
Cuts at: 2769 3075 3087 3173 4225 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 886 467 449 335 316 307 306
246 188 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1373 1373
Size: 4673
BtgI C'CryG_G
Cuts at: 107 310 1194 1334 107
Size: 203 884 140 3446
Fragments arranged by size:
3446 884 203 140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 883 2623 2804 2967 883
Size: 1740 181 163 2589
Fragments arranged by size:
2589 1740 181 163
BtsI GCAGTG_nn'
Cuts at: 228 411 503 993 1916 228
Size: 183 92 490 923 2985
Fragments arranged by size:
2985 923 490 183 92
DraI TTT'AAA
Cuts at: 1672 1672
Size: 4673
DraIII CAC_nnn'GTG
Cuts at: 456 456
Size: 4673
DrdI GACnn_nn'nnGTC
Cuts at: 2379 3723 2379
Size: 1344 3329
EaeI y'GGCC_r
Cuts at: 1211 2258 2432 2823 2850 4195 1211
Size: 1047 174 391 27 1345 1689
Fragments arranged by size:
1689 1345 1047 391 174 27
EagI C'GGCC_G
Cuts at: 2258 2258
Size: 4673
EarI CTCTTCn'nnn_
Cuts at: 659 1486 1586 2696 2906 659
Size: 827 100 1110 210 2426
Fragments arranged by size:
2426 1110 827 210 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1525 2881 3623 3769 4483 1525
Size: 1356 742 146 714 1715
Fragments arranged by size:
1715 1356 742 714 146
EcoICRI GAG'CTC
Cuts at: 738 1685 738
Size: 947 3726
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 1006 1641 2498 2930 3283 3945 565
Size: 441 635 857 432 353 662 1293
Fragments arranged by size:
1293 857 662 635 441 432 353
EcoO109I rG'GnC_Cy
Cuts at: 365 534 585 681 786 1521 1522 1812 365
Size: 169 51 96 105 735 1 290 3226
Fragments arranged by size:
3226 735 290 169 105 96 51 1
EcoRI G'AATT_C
Cuts at: 1677 1677
Size: 4673
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 399 2692 399
Size: 2293 2380
FspI TGC'GCA
Cuts at: 1445 2454 1445
Size: 1009 3664
HaeII r_GCGC'y
Cuts at: 851 2107 2355 3585 4356 851
Size: 1256 248 1230 771 1168
Fragments arranged by size:
1256 1230 1168 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 349 381 1645 1677 2515 2545 2547 2577 349
Size: 32 1264 32 838 30 2 30 2445
Fragments arranged by size:
2445 1264 838 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1712 1712
Size: 4673
KasI G'GCGC_C
Cuts at: 2103 2351 2103
Size: 248 4425
KpnI G_GTAC'C
Cuts at: 1693 1693
Size: 4673
MluI A'CGCG_T
Cuts at: 4106 4106
Size: 4673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3432 3616 3432
Size: 184 4489
MscI TGG'CCA
Cuts at: 1213 2434 1213
Size: 1221 3452
MslI CAynn'nnrTG
Cuts at: 170 868 1402 2662 2789 170
Size: 698 534 1260 127 2054
Fragments arranged by size:
2054 1260 698 534 127
MspA1I CmG'CkG
Cuts at: 898 1196 1784 2098 2458 3242 3487 898
Size: 298 588 314 360 784 245 2084
Fragments arranged by size:
2084 784 588 360 314 298 245
NaeI GCC'GGC
Cuts at: 2854 2854
Size: 4673
NarI GG'CG_CC
Cuts at: 2104 2352 2104
Size: 248 4425
NcoI C'CATG_G
Cuts at: 107 310 1334 107
Size: 203 1024 3446
Fragments arranged by size:
3446 1024 203
NgoMIV G'CCGG_C
Cuts at: 2852 2852
Size: 4673
NspI r_CATG'y
Cuts at: 1726 2226 3829 1726
Size: 500 1603 2570
Fragments arranged by size:
2570 1603 500
PciI A'CATG_T
Cuts at: 3825 3825
Size: 4673
PflMI CCAn_nnn'nTGG
Cuts at: 456 1545 456
Size: 1089 3584
PfoI T'CCnGG_A
Cuts at: 530 581 875 530
Size: 51 294 4328
Fragments arranged by size:
4328 294 51
PmeI GTTT'AAAC
Cuts at: 1672 1672
Size: 4673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3119 3151 3119
Size: 32 4641
PpuMI rG'GwC_Cy
Cuts at: 365 534 585 681 365
Size: 169 51 96 4357
Fragments arranged by size:
4357 169 96 51
PspOMI G'GGCC_C
Cuts at: 1222 1521 1222
Size: 299 4374
PstI C_TGCA'G
Cuts at: 235 248 734 1720 2405 235
Size: 13 486 986 685 2503
Fragments arranged by size:
2503 986 685 486 13
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4673
PvuII CAG'CTG
Cuts at: 898 2098 2458 898
Size: 1200 360 3113
Fragments arranged by size:
3113 1200 360
RsrII CG'GwC_CG
Cuts at: 2868 2868
Size: 4673
SacI G_AGCT'C
Cuts at: 740 1687 740
Size: 947 3726
SacII CC_GC'GG
Cuts at: 1197 1197
Size: 4673
SalI G'TCGA_C
Cuts at: 1710 1710
Size: 4673
SapI GCTCTTCn'nnn_
Cuts at: 1486 1586 2696 2906 1486
Size: 100 1110 210 3253
Fragments arranged by size:
3253 1110 210 100
SbfI CC_TGCA'GG
Cuts at: 734 1720 734
Size: 986 3687
ScaI AGT'ACT
Cuts at: 4190 4190
Size: 4673
SfcI C'TryA_G
Cuts at: 33 231 244 730 1400 1716 2401 3369 3560
Size: 198 13 486 670 316 685 968 191
Cuts at: 3560 33
Size: 1146
Fragments arranged by size:
1146 968 685 670 486 316 198 191
13
SfoI GGC'GCC
Cuts at: 2105 2353 2105
Size: 248 4425
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
SmaI CCC'GGG
Cuts at: 1695 1695
Size: 4673
SmlI C'TyrA_G
Cuts at: 316 1465 1828 2743 3457 3719 316
Size: 1149 363 915 714 262 1270
Fragments arranged by size:
1270 1149 915 714 363 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4673
SphI G_CATG'C
Cuts at: 1726 2226 1726
Size: 500 4173
StyI C'CwwG_G
Cuts at: 107 310 1098 1334 1807 2161 107
Size: 203 788 236 473 354 2619
Fragments arranged by size:
2619 788 473 354 236 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 463 559 2256 2389 2848 3057 463
Size: 96 1697 133 459 209 2079
Fragments arranged by size:
2079 1697 459 209 133 96
TatI w'GTAC_w
Cuts at: 140 1553 4188 140
Size: 1413 2635 625
Fragments arranged by size:
2635 1413 625
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4190 4222 4190
Size: 32 4641
Tth111I GACn'n_nGTC
Cuts at: 2470 2470
Size: 4673
XbaI T'CTAG_A
Cuts at: 1704 1704
Size: 4673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1341 2168 1341
Size: 827 3846
XmaI C'CCGG_G
Cuts at: 1693 1693
Size: 4673
XmnI GAAnn'nnTTC
Cuts at: 1153 1153
Size: 4673
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AgeI AlfI AloI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BssHII
BstEII BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI