Restriction map for
pF1KB8720
(Circular) (Six-base) MAPSORT of: pF1KB8720.seq Check: 8233 from: 1 to: 5273
pF1KB8720 5273 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 2311 2311
Size: 5273
Acc65I G'GTAC_C
Cuts at: 2289 2289
Size: 5273
AclI AA'CG_TT
Cuts at: 5113 5113
Size: 5273
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 1006 3098 3530 3883 4545 449
Size: 557 2092 432 353 662 1177
Fragments arranged by size:
2092 1177 662 557 432 353
AflIII A'CryG_T
Cuts at: 2085 2625 4425 4706 2085
Size: 540 1800 281 2652
Fragments arranged by size:
2652 1800 540 281
AgeI A'CCGG_T
Cuts at: 2172 2172
Size: 5273
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2233 2265 2233
Size: 32 5241
AlwNI CAG_nnn'CTG
Cuts at: 1216 1341 2026 2578 4016 1216
Size: 125 685 552 1438 2473
Fragments arranged by size:
2473 1438 685 552 125
ApaI G_GGCC'C
Cuts at: 578 578
Size: 5273
ApaLI G'TGCA_C
Cuts at: 1077 1539 4111 1077
Size: 462 2572 2239
Fragments arranged by size:
2572 2239 462
ApoI r'AATT_y
Cuts at: 154 1114 1419 1463 2277 4626 4882 154
Size: 960 305 44 814 2349 256 545
Fragments arranged by size:
2349 960 814 545 305 256 44
AseI AT'TA_AT
Cuts at: 20 20
Size: 5273
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5273
AvaI C'yCGr_G
Cuts at: 650 742 764 903 1044 1934 2293 650
Size: 92 22 139 141 890 359 3630
Fragments arranged by size:
3630 890 359 141 139 92 22
BamHI G'GATC_C
Cuts at: 2298 2298
Size: 5273
BanI G'GyrC_C
Cuts at: 631 654 1284 2289 2703 2951 2986 631
Size: 23 630 1005 414 248 35 2918
Fragments arranged by size:
2918 1005 630 414 248 35 23
BanII G_rGCy'C
Cuts at: 325 578 1714 1754 2203 2224 2287 3317 325
Size: 253 1136 40 449 21 63 1030 2281
Fragments arranged by size:
2281 1136 1030 449 253 63 40 21
BbeI G_GCGC'C
Cuts at: 658 2707 2955 658
Size: 2049 248 2976
Fragments arranged by size:
2976 2049 248
BbsI GAAGACnn'nnnn_
Cuts at: 134 170 201 437 997 134
Size: 36 31 236 560 4410
Fragments arranged by size:
4410 560 236 36 31
BbvCI CC'TCA_GC
Cuts at: 1293 1293
Size: 5273
BciVI GTATCCnnnnn_n'
Cuts at: 3171 4227 5273 3171
Size: 1056 1046 3171
Fragments arranged by size:
3171 1056 1046
BclI T'GATC_A
Cuts at: 2793 2793
Size: 5273
BglI GCCn_nnn'nGGC
Cuts at: 941 941
Size: 5273
BglII A'GATC_T
Cuts at: 2788 4825 2788
Size: 2037 3236
BlpI GC'TnA_GC
Cuts at: 1615 2218 2385 1615
Size: 603 167 4503
Fragments arranged by size:
4503 603 167
Bme1580I G_kGCm'C
Cuts at: 578 636 1081 1524 1543 2898 2991 4115 578
Size: 58 445 443 19 1355 93 1124 1736
Fragments arranged by size:
1736 1355 1124 445 443 93 58 19
BmgBI CAC'GTC
Cuts at: 527 1544 527
Size: 1017 4256
BmrI ACTGGGnnnn_n'
Cuts at: 2485 2566 2901 5045 2485
Size: 81 335 2144 2713
Fragments arranged by size:
2713 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2222 3640 2222
Size: 1418 3855
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 339 1332 1868 2003 2123 339
Size: 993 536 135 120 3489
Fragments arranged by size:
3489 993 536 135 120
Bpu10I CC'TnA_GC
Cuts at: 1293 2197 1293
Size: 904 4369
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2245 2449 3328 4042 4340 2245
Size: 204 879 714 298 3178
Fragments arranged by size:
3178 879 714 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 4977 4977
Size: 5273
BsaAI yAC'GTr
Cuts at: 2088 2626 2088
Size: 538 4735
BsaBI GATnn'nnATC
Cuts at: 6 2787 4824 6
Size: 2781 2037 455
Fragments arranged by size:
2781 2037 455
BsaHI Gr'CG_yC
Cuts at: 655 2704 2952 3654 5150 655
Size: 2049 248 702 1496 778
Fragments arranged by size:
2049 1496 778 702 248
BsaWI w'CCGG_w
Cuts at: 270 1320 2172 2462 2983 3661 4072 4219 4493
Size: 1050 852 290 521 678 411 147 274
Cuts at: 4493 270
Size: 1050
Fragments arranged by size:
1050 1050 852 678 521 411 290 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2121 2151 2185 2215 2121
Size: 30 34 30 5179
Fragments arranged by size:
5179 34 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 597 1032 1838 2248 597
Size: 435 806 410 3622
Fragments arranged by size:
3622 806 435 410
BseYI C'CCAG_C
Cuts at: 570 622 855 1086 2698 3456 4121 4529 570
Size: 52 233 231 1612 758 665 408 1314
Fragments arranged by size:
1612 1314 758 665 408 233 231 52
BsiEI CG_ry'CG
Cuts at: 104 926 1531 2815 2861 4091 104
Size: 822 605 1284 46 1230 1286
Fragments arranged by size:
1286 1284 1230 822 605 46
BsiHKAI G_wGCw'C
Cuts at: 1081 1543 2203 2287 3065 3255 4115 1081
Size: 462 660 84 778 190 860 2239
Fragments arranged by size:
2239 860 778 660 462 190 84
BsmBI CGTCTCn'nnnn_
Cuts at: 533 1731 533
Size: 1198 4075
Bsp1286I G_dGCh'C
Cuts at: 325 578 636 1081 1524 1543 1714 1754 2203
Size: 253 58 445 443 19 171 40 449
Cuts at: 2203 2224 2287 2898 2991 3065 3255 3317 4115
Size: 21 63 611 93 74 190 62 798
Cuts at: 4115 325
Size: 1483
Fragments arranged by size:
1483 798 611 449 445 443 253 190
171 93 74 63 62 58 40 21
19
BspEI T'CCGG_A
Cuts at: 1320 1320
Size: 5273
BspHI T'CATG_A
Cuts at: 2126 3705 2126
Size: 1579 3694
BspMI ACCTGCnnnn'nnnn_
Cuts at: 906 2323 2537 2839 3220 906
Size: 1417 214 302 381 2959
Fragments arranged by size:
2959 1417 381 302 214
BsrBI CCG'CTC
Cuts at: 50 769 1061 3565 3619 5103 5268 50
Size: 719 292 2504 54 1484 165 55
Fragments arranged by size:
2504 1484 719 292 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1775 3185 1775
Size: 1410 3863
BsrFI r'CCGG_y
Cuts at: 921 1655 2172 3271 3452 921
Size: 734 517 1099 181 2742
Fragments arranged by size:
2742 1099 734 517 181
BsrGI T'GTAC_A
Cuts at: 353 353
Size: 5273
BssSI C'ACGA_G
Cuts at: 3544 4252 3544
Size: 708 4565
BstAPI GCAn_nnn'nTGC
Cuts at: 1072 2650 1072
Size: 1578 3695
BstBI TT'CG_AA
Cuts at: 3634 3634
Size: 5273
BstXI CCAn_nnnn'nTGG
Cuts at: 1707 1821 1707
Size: 114 5159
BstYI r'GATC_y
Cuts at: 1 2098 2298 2765 2788 3123 3369 3675 3687
Size: 2097 200 467 23 335 246 306 12
Cuts at: 3687 3773 4825 1
Size: 86 1052 449
Fragments arranged by size:
2097 1052 467 449 335 306 246 200
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1865 2225 1865
Size: 360 4913
BtgI C'CryG_G
Cuts at: 107 1706 107
Size: 1599 3674
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3223 3404 3567 3223
Size: 181 163 4929
Fragments arranged by size:
4929 181 163
BtsI GCAGTG_nn'
Cuts at: 701 1097 2516 701
Size: 396 1419 3458
Fragments arranged by size:
3458 1419 396
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1935 1970 1935
Size: 35 5238
DraI TTT'AAA
Cuts at: 1461 2272 1461
Size: 811 4462
DraIII CAC_nnn'GTG
Cuts at: 1627 1627
Size: 5273
DrdI GACnn_nn'nnGTC
Cuts at: 2979 4323 2979
Size: 1344 3929
EaeI y'GGCC_r
Cuts at: 143 329 923 1195 1332 1528 2159 2858 3032
Size: 186 594 272 137 196 631 699 174
Cuts at: 3032 3423 3450 4795 143
Size: 391 27 1345 621
Fragments arranged by size:
1345 699 631 621 594 391 272 196
186 174 137 27
EagI C'GGCC_G
Cuts at: 923 1528 2858 923
Size: 605 1330 3338
Fragments arranged by size:
3338 1330 605
EarI CTCTTCn'nnn_
Cuts at: 1985 3296 3506 1985
Size: 1311 210 3752
Fragments arranged by size:
3752 1311 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3481 4223 4369 5083 3481
Size: 742 146 714 3671
Fragments arranged by size:
3671 742 714 146
EcoICRI GAG'CTC
Cuts at: 2201 2285 2201
Size: 84 5189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 339 449 1006 1332 1868 2003 2123 3098 3530
Size: 110 557 326 536 135 120 975 432
Cuts at: 3530 3883 4545 339
Size: 353 662 1067
Fragments arranged by size:
1067 975 662 557 536 432 353 326
135 120 110
EcoO109I rG'GnC_Cy
Cuts at: 574 575 661 1048 1180 1213 2412 574
Size: 1 86 387 132 33 1199 3435
Fragments arranged by size:
3435 1199 387 132 86 33 1
EcoRI G'AATT_C
Cuts at: 2277 2277
Size: 5273
EcoRV GAT'ATC
Cuts at: 1992 1992
Size: 5273
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 151 3292 151
Size: 3141 2132
FspI TGC'GCA
Cuts at: 3054 3054
Size: 5273
HaeII r_GCGC'y
Cuts at: 658 1662 2707 2955 4185 4956 658
Size: 1004 1045 248 1230 771 975
Fragments arranged by size:
1230 1045 1004 975 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 853 885 2121 2153 2185 2217 3115 3145 3147
Size: 32 1236 32 32 32 898 30 2
Cuts at: 3147 3177 853
Size: 30 2949
Fragments arranged by size:
2949 1236 898 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2312 2312
Size: 5273
HindIII A'AGCT_T
Cuts at: 1510 1510
Size: 5273
KasI G'GCGC_C
Cuts at: 654 2703 2951 654
Size: 2049 248 2976
Fragments arranged by size:
2976 2049 248
KpnI G_GTAC'C
Cuts at: 2293 2293
Size: 5273
MfeI C'AATT_G
Cuts at: 1375 1375
Size: 5273
MluI A'CGCG_T
Cuts at: 4706 4706
Size: 5273
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4032 4216 4032
Size: 184 5089
MscI TGG'CCA
Cuts at: 2161 3034 2161
Size: 873 4400
MslI CAynn'nnrTG
Cuts at: 878 3262 3389 878
Size: 2384 127 2762
Fragments arranged by size:
2762 2384 127
MspA1I CmG'CkG
Cuts at: 859 1270 1527 2217 2384 2698 3058 3842 4087
Size: 411 257 690 167 314 360 784 245
Cuts at: 4087 859
Size: 2045
Fragments arranged by size:
2045 784 690 411 360 314 257 245
167
NaeI GCC'GGC
Cuts at: 923 1657 3454 923
Size: 734 1797 2742
Fragments arranged by size:
2742 1797 734
NarI GG'CG_CC
Cuts at: 655 2704 2952 655
Size: 2049 248 2976
Fragments arranged by size:
2976 2049 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5273
NgoMIV G'CCGG_C
Cuts at: 921 1655 3452 921
Size: 734 1797 2742
Fragments arranged by size:
2742 1797 734
NotI GC'GGCC_GC
Cuts at: 1528 1528
Size: 5273
NspI r_CATG'y
Cuts at: 2326 2826 4429 2326
Size: 500 1603 3170
Fragments arranged by size:
3170 1603 500
PasI CC'CwG_GG
Cuts at: 689 796 1700 689
Size: 107 904 4262
Fragments arranged by size:
4262 904 107
PciI A'CATG_T
Cuts at: 4425 4425
Size: 5273
PflMI CCAn_nnn'nTGG
Cuts at: 450 450
Size: 5273
PfoI T'CCnGG_A
Cuts at: 841 841
Size: 5273
PmeI GTTT'AAAC
Cuts at: 2272 2272
Size: 5273
PmlI CAC'GTG
Cuts at: 2088 2088
Size: 5273
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 3719
Size: 32 5241
PpuMI rG'GwC_Cy
Cuts at: 1180 1180
Size: 5273
PspOMI G'GGCC_C
Cuts at: 574 574
Size: 5273
PspXI vC'TCGA_Gb
Cuts at: 764 764
Size: 5273
PstI C_TGCA'G
Cuts at: 1212 2320 3005 1212
Size: 1108 685 3480
Fragments arranged by size:
3480 1108 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5273
PvuII CAG'CTG
Cuts at: 859 2217 2698 3058 859
Size: 1358 481 360 3074
Fragments arranged by size:
3074 1358 481 360
RsrII CG'GwC_CG
Cuts at: 3468 3468
Size: 5273
SacI G_AGCT'C
Cuts at: 2203 2287 2203
Size: 84 5189
SalI G'TCGA_C
Cuts at: 2310 2310
Size: 5273
SanDI GG'GwC_CC
Cuts at: 1180 1180
Size: 5273
SapI GCTCTTCn'nnn_
Cuts at: 1985 3296 3506 1985
Size: 1311 210 3752
Fragments arranged by size:
3752 1311 210
SbfI CC_TGCA'GG
Cuts at: 1212 2320 1212
Size: 1108 4165
ScaI AGT'ACT
Cuts at: 1917 4790 1917
Size: 2873 2400
SfcI C'TryA_G
Cuts at: 33 1208 2093 2316 3001 3969 4160 33
Size: 1175 885 223 685 968 191 1146
Fragments arranged by size:
1175 1146 968 885 685 223 191
SfoI GGC'GCC
Cuts at: 656 2705 2953 656
Size: 2049 248 2976
Fragments arranged by size:
2976 2049 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5273
SgrAI Cr'CCGG_yG
Cuts at: 2172 2172
Size: 5273
SmaI CCC'GGG
Cuts at: 652 744 905 2295 652
Size: 92 161 1390 3630
Fragments arranged by size:
3630 1390 161 92
SmlI C'TyrA_G
Cuts at: 764 2260 2428 3343 4057 4319 764
Size: 1496 168 915 714 262 1718
Fragments arranged by size:
1718 1496 915 714 262 168
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5273
SphI G_CATG'C
Cuts at: 2326 2826 2326
Size: 500 4773
StuI AGG'CCT
Cuts at: 2112 2112
Size: 5273
StyI C'CwwG_G
Cuts at: 107 2407 2761 107
Size: 2300 354 2619
Fragments arranged by size:
2619 2300 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 574 1321 2856 2989 3448 3657 574
Size: 747 1535 133 459 209 2190
Fragments arranged by size:
2190 1535 747 459 209 133
TatI w'GTAC_w
Cuts at: 353 1915 4788 353
Size: 1562 2873 838
Fragments arranged by size:
2873 1562 838
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4790 4822 4790
Size: 32 5241
Tth111I GACn'n_nGTC
Cuts at: 3070 3070
Size: 5273
XbaI T'CTAG_A
Cuts at: 2304 2304
Size: 5273
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2768 2768
Size: 5273
XhoI C'TCGA_G
Cuts at: 764 764
Size: 5273
XmaI C'CCGG_G
Cuts at: 650 742 903 2293 650
Size: 92 161 1390 3630
Fragments arranged by size:
3630 1390 161 92
XmnI GAAnn'nnTTC
Cuts at: 131 131
Size: 5273
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BcgI BfrBI BmtI BsgI BsiWI BsmI
BssHII BstEII BstZ17I ClaI EcoNI FseI FspAI HpaI
NdeI NheI NruI NsiI PacI PshAI PsiI PsrI
SacII SexAI SfiI SnaBI SrfI SspI SwaI ZraI