Restriction map for
pF1KB8718
(Circular) (Six-base) MAPSORT of: pF1KB8718.seq Check: 333 from: 1 to: 4928
pF1KB8718 4928 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 703 703
Size: 4928
AatII G_ACGT'C
Cuts at: 1373 1709 1373
Size: 336 4592
AccI GT'mk_AC
Cuts at: 558 1966 558
Size: 1408 3520
Acc65I G'GTAC_C
Cuts at: 1944 1944
Size: 4928
AclI AA'CG_TT
Cuts at: 4768 4768
Size: 4928
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1194 1348 1363 1756 2753 3185 3538 4200 1194
Size: 154 15 393 997 432 353 662 1922
Fragments arranged by size:
1922 997 662 432 393 353 154 15
AflIII A'CryG_T
Cuts at: 1046 1604 1895 2280 4080 4361 1046
Size: 558 291 385 1800 281 1613
Fragments arranged by size:
1800 1613 558 385 291 281
AleI CACnn'nnGTG
Cuts at: 694 694
Size: 4928
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 712 913 1003 1375 1687 712
Size: 201 90 372 312 3953
Fragments arranged by size:
3953 372 312 201 90
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1033 1065 1033
Size: 32 4896
AlwNI CAG_nnn'CTG
Cuts at: 2233 3671 2233
Size: 1438 3490
ApaLI G'TGCA_C
Cuts at: 780 3766 780
Size: 2986 1942
ApoI r'AATT_y
Cuts at: 1932 4281 4537 1932
Size: 2349 256 2323
Fragments arranged by size:
2349 2323 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4928
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4928
AvaI C'yCGr_G
Cuts at: 218 340 1097 1442 1948 218
Size: 122 757 345 506 3198
Fragments arranged by size:
3198 757 506 345 122
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 669 702 669
Size: 33 4895
BamHI G'GATC_C
Cuts at: 1918 1953 1918
Size: 35 4893
BanI G'GyrC_C
Cuts at: 502 794 993 1944 2358 2606 2641 502
Size: 292 199 951 414 248 35 2789
Fragments arranged by size:
2789 951 414 292 248 199 35
BanII G_rGCy'C
Cuts at: 146 1942 2972 146
Size: 1796 1030 2102
Fragments arranged by size:
2102 1796 1030
BbeI G_GCGC'C
Cuts at: 506 2362 2610 506
Size: 1856 248 2824
Fragments arranged by size:
2824 1856 248
BbvCI CC'TCA_GC
Cuts at: 1820 1820
Size: 4928
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1095 1129 1338 1372 1095
Size: 34 209 34 4651
Fragments arranged by size:
4651 209 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2826 3882 4928 2826
Size: 1056 1046 2826
Fragments arranged by size:
2826 1056 1046
BclI T'GATC_A
Cuts at: 2448 2448
Size: 4928
BglII A'GATC_T
Cuts at: 2443 4480 2443
Size: 2037 2891
BlpI GC'TnA_GC
Cuts at: 820 2040 820
Size: 1220 3708
Bme1580I G_kGCm'C
Cuts at: 784 1245 1400 1640 2553 2646 3770 784
Size: 461 155 240 913 93 1124 1942
Fragments arranged by size:
1942 1124 913 461 240 155 93
BmgBI CAC'GTC
Cuts at: 330 930 330
Size: 600 4328
BmrI ACTGGGnnnn_n'
Cuts at: 533 2140 2221 2556 4700 533
Size: 1607 81 335 2144 761
Fragments arranged by size:
2144 1607 761 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 777 3295 777
Size: 2518 2410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 991 1216 1551 1747 553
Size: 438 225 335 196 3734
Fragments arranged by size:
3734 438 335 225 196
Bpu10I CC'TnA_GC
Cuts at: 845 911 1580 1820 845
Size: 66 669 240 3953
Fragments arranged by size:
3953 669 240 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 2104 2983 3697 3995 130
Size: 1974 879 714 298 1063
Fragments arranged by size:
1974 1063 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4632 4632
Size: 4928
BsaAI yAC'GTr
Cuts at: 2281 2281
Size: 4928
BsaBI GATnn'nnATC
Cuts at: 6 1076 2442 4479 6
Size: 1070 1366 2037 455
Fragments arranged by size:
2037 1366 1070 455
BsaHI Gr'CG_yC
Cuts at: 503 1063 1204 1370 1706 2359 2607 3309 4805
Size: 560 141 166 336 653 248 702 1496
Cuts at: 4805 503
Size: 626
Fragments arranged by size:
1496 702 653 626 560 336 248 166
141
BsaWI w'CCGG_w
Cuts at: 767 1058 1847 2117 2638 3316 3727 3874 4148
Size: 291 789 270 521 678 411 147 274
Cuts at: 4148 767
Size: 1547
Fragments arranged by size:
1547 789 678 521 411 291 274 270
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 178 208 256 286 580 610 1033 1063 1723
Size: 30 48 30 294 30 423 30 660
Cuts at: 1723 1753 178
Size: 30 3353
Fragments arranged by size:
3353 660 423 294 48 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 277 1213 1744 202
Size: 75 936 531 3386
Fragments arranged by size:
3386 936 531 75
BseYI C'CCAG_C
Cuts at: 292 369 374 673 2353 3111 3776 4184 292
Size: 77 5 299 1680 758 665 408 1036
Fragments arranged by size:
1680 1036 758 665 408 299 77 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 731 737 755 1412 1861 731
Size: 6 18 657 449 3798
Fragments arranged by size:
3798 657 449 18 6
BsiEI CG_ry'CG
Cuts at: 104 768 810 2470 2516 3746 104
Size: 664 42 1660 46 1230 1286
Fragments arranged by size:
1660 1286 1230 664 46 42
BsiHKAI G_wGCw'C
Cuts at: 146 701 784 902 1633 1942 2720 2910 3770
Size: 555 83 118 731 309 778 190 860
Cuts at: 3770 146
Size: 1304
Fragments arranged by size:
1304 860 778 731 555 309 190 118
83
BsmBI CGTCTCn'nnnn_
Cuts at: 1195 1195
Size: 4928
Bsp1286I G_dGCh'C
Cuts at: 146 701 784 902 1245 1400 1633 1640 1942
Size: 555 83 118 343 155 233 7 302
Cuts at: 1942 2553 2646 2720 2910 2972 3770 146
Size: 611 93 74 190 62 798 1304
Fragments arranged by size:
1304 798 611 555 343 302 233 190
155 118 93 83 74 62 7
BspEI T'CCGG_A
Cuts at: 1058 1847 1058
Size: 789 4139
BspHI T'CATG_A
Cuts at: 1247 3360 1247
Size: 2113 2815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 703 1978 2192 2494 2875 703
Size: 1275 214 302 381 2756
Fragments arranged by size:
2756 1275 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3220 3274 4758 4923 50
Size: 3170 54 1484 165 55
Fragments arranged by size:
3170 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 240 2840 240
Size: 2600 2328
BsrFI r'CCGG_y
Cuts at: 791 1126 2926 3107 791
Size: 335 1800 181 2612
Fragments arranged by size:
2612 1800 335 181
BssSI C'ACGA_G
Cuts at: 3199 3907 3199
Size: 708 4220
BstAPI GCAn_nnn'nTGC
Cuts at: 2305 2305
Size: 4928
BstBI TT'CG_AA
Cuts at: 3289 3289
Size: 4928
BstXI CCAn_nnnn'nTGG
Cuts at: 138 235 648 1083 138
Size: 97 413 435 3983
Fragments arranged by size:
3983 435 413 97
BstYI r'GATC_y
Cuts at: 1 1722 1918 1953 2420 2443 2778 3024 3330
Size: 1721 196 35 467 23 335 246 306
Cuts at: 3330 3342 3428 4480 1
Size: 12 86 1052 449
Fragments arranged by size:
1721 1052 467 449 335 306 246 196
86 35 23 12
Bsu36I CC'TnA_GG
Cuts at: 299 859 1414 1833 299
Size: 560 555 419 3394
Fragments arranged by size:
3394 560 555 419
BtgI C'CryG_G
Cuts at: 107 474 1778 107
Size: 367 1304 3257
Fragments arranged by size:
3257 1304 367
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1520 2878 3059 3222 1520
Size: 1358 181 163 3226
Fragments arranged by size:
3226 1358 181 163
BtsI GCAGTG_nn'
Cuts at: 742 1400 2171 742
Size: 658 771 3499
Fragments arranged by size:
3499 771 658
DraI TTT'AAA
Cuts at: 1927 1927
Size: 4928
DraIII CAC_nnn'GTG
Cuts at: 1179 1179
Size: 4928
DrdI GACnn_nn'nnGTC
Cuts at: 772 1296 2634 3978 772
Size: 524 1338 1344 1722
Fragments arranged by size:
1722 1344 1338 524
EaeI y'GGCC_r
Cuts at: 197 348 650 1148 1781 2513 2687 3078 3105
Size: 151 302 498 633 732 174 391 27
Cuts at: 3105 4450 197
Size: 1345 675
Fragments arranged by size:
1345 732 675 633 498 391 302 174
151 27
EagI C'GGCC_G
Cuts at: 2513 2513
Size: 4928
EarI CTCTTCn'nnn_
Cuts at: 1339 2951 3161 1339
Size: 1612 210 3106
Fragments arranged by size:
3106 1612 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1636 3136 3878 4024 4738 1636
Size: 1500 742 146 714 1826
Fragments arranged by size:
1826 1500 742 714 146
EcoICRI GAG'CTC
Cuts at: 144 1940 144
Size: 1796 3132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 991 1194 1216 1348 1363 1551 1747 1756
Size: 438 203 22 132 15 188 196 9
Cuts at: 1756 2753 3185 3538 4200 553
Size: 997 432 353 662 1281
Fragments arranged by size:
1281 997 662 438 432 353 203 196
188 132 22 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1212 1212
Size: 4928
EcoO109I rG'GnC_Cy
Cuts at: 336 528 618 2067 336
Size: 192 90 1449 3197
Fragments arranged by size:
3197 1449 192 90
EcoRI G'AATT_C
Cuts at: 1932 1932
Size: 4928
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 978 2947 978
Size: 1969 2959
FspI TGC'GCA
Cuts at: 2709 2709
Size: 4928
HaeII r_GCGC'y
Cuts at: 270 506 2362 2610 3840 4611 270
Size: 236 1856 248 1230 771 587
Fragments arranged by size:
1856 1230 771 587 248 236
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 178 210 256 288 1436 1468 2770 2800 2802
Size: 32 46 32 1148 32 1302 30 2
Cuts at: 2802 2832 178
Size: 30 2274
Fragments arranged by size:
2274 1302 1148 46 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1461 1967 1461
Size: 506 4422
KasI G'GCGC_C
Cuts at: 502 2358 2606 502
Size: 1856 248 2824
Fragments arranged by size:
2824 1856 248
KpnI G_GTAC'C
Cuts at: 1948 1948
Size: 4928
MluI A'CGCG_T
Cuts at: 4361 4361
Size: 4928
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 788 3687 3871 788
Size: 2899 184 1845
Fragments arranged by size:
2899 1845 184
MscI TGG'CCA
Cuts at: 350 652 1783 2689 350
Size: 302 1131 906 2589
Fragments arranged by size:
2589 1131 906 302
MslI CAynn'nnrTG
Cuts at: 694 895 1252 2917 3044 694
Size: 201 357 1665 127 2578
Fragments arranged by size:
2578 1665 357 201 127
MspA1I CmG'CkG
Cuts at: 627 721 852 1501 2039 2353 2713 3497 3742
Size: 94 131 649 538 314 360 784 245
Cuts at: 3742 627
Size: 1813
Fragments arranged by size:
1813 784 649 538 360 314 245 131
94
NaeI GCC'GGC
Cuts at: 1128 3109 1128
Size: 1981 2947
NarI GG'CG_CC
Cuts at: 503 2359 2607 503
Size: 1856 248 2824
Fragments arranged by size:
2824 1856 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4928
NgoMIV G'CCGG_C
Cuts at: 1126 3107 1126
Size: 1981 2947
NspI r_CATG'y
Cuts at: 1287 1608 1899 1981 2481 4084 1287
Size: 321 291 82 500 1603 2131
Fragments arranged by size:
2131 1603 500 321 291 82
PasI CC'CwG_GG
Cuts at: 420 420
Size: 4928
PciI A'CATG_T
Cuts at: 1604 1895 4080 1604
Size: 291 2185 2452
Fragments arranged by size:
2452 2185 291
PflMI CCAn_nnn'nTGG
Cuts at: 420 673 420
Size: 253 4675
PmeI GTTT'AAAC
Cuts at: 1927 1927
Size: 4928
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1033 1065 3374 3406 1033
Size: 32 2309 32 2555
Fragments arranged by size:
2555 2309 32 32
PpuMI rG'GwC_Cy
Cuts at: 336 528 336
Size: 192 4736
PshAI GACnn'nnGTC
Cuts at: 1480 1480
Size: 4928
PstI C_TGCA'G
Cuts at: 632 1975 2660 632
Size: 1343 685 2900
Fragments arranged by size:
2900 1343 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4928
PvuII CAG'CTG
Cuts at: 627 721 852 1501 2353 2713 627
Size: 94 131 649 852 360 2842
Fragments arranged by size:
2842 852 649 360 131 94
RsrII CG'GwC_CG
Cuts at: 3123 3123
Size: 4928
SacI G_AGCT'C
Cuts at: 146 1942 146
Size: 1796 3132
SalI G'TCGA_C
Cuts at: 1965 1965
Size: 4928
SapI GCTCTTCn'nnn_
Cuts at: 1339 2951 3161 1339
Size: 1612 210 3106
Fragments arranged by size:
3106 1612 210
SbfI CC_TGCA'GG
Cuts at: 1975 1975
Size: 4928
ScaI AGT'ACT
Cuts at: 4445 4445
Size: 4928
SfcI C'TryA_G
Cuts at: 33 622 628 1971 2656 3624 3815 33
Size: 589 6 1343 685 968 191 1146
Fragments arranged by size:
1343 1146 968 685 589 191 6
SfoI GGC'GCC
Cuts at: 504 2360 2608 504
Size: 1856 248 2824
Fragments arranged by size:
2824 1856 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4928
SmaI CCC'GGG
Cuts at: 220 1950 220
Size: 1730 3198
SmlI C'TyrA_G
Cuts at: 145 340 2083 2998 3712 3974 145
Size: 195 1743 915 714 262 1099
Fragments arranged by size:
1743 1099 915 714 262 195
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4928
SphI G_CATG'C
Cuts at: 1981 2481 1981
Size: 500 4428
StyI C'CwwG_G
Cuts at: 107 641 866 1811 2062 2416 107
Size: 534 225 945 251 354 2619
Fragments arranged by size:
2619 945 534 354 251 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 697 2511 2644 3103 3312 697
Size: 1814 133 459 209 2313
Fragments arranged by size:
2313 1814 459 209 133
TatI w'GTAC_w
Cuts at: 974 4443 974
Size: 3469 1459
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4445 4477 4445
Size: 32 4896
Tth111I GACn'n_nGTC
Cuts at: 1705 2725 1705
Size: 1020 3908
XbaI T'CTAG_A
Cuts at: 1959 1959
Size: 4928
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2423 2423
Size: 4928
XhoI C'TCGA_G
Cuts at: 340 340
Size: 4928
XmaI C'CCGG_G
Cuts at: 218 1948 218
Size: 1730 3198
XmnI GAAnn'nnTTC
Cuts at: 1048 1048
Size: 4928
ZraI GAC'GTC
Cuts at: 1371 1707 1371
Size: 336 4592
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI ApaI AscI AvrII BbsI
BfrBI BglI BmtI BsiWI BsmI BsrGI BssHII BstEII
BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI