Restriction map for
pF1KB8715
(Circular) (Six-base) MAPSORT of: pF1KB8715.seq Check: 3688 from: 1 to: 4271
pF1KB8715 4271 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 243 678 243
Size: 435 3836
AccI GT'mk_AC
Cuts at: 1309 1309
Size: 4271
Acc65I G'GTAC_C
Cuts at: 1287 1287
Size: 4271
AclI AA'CG_TT
Cuts at: 4111 4111
Size: 4271
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2096 2528 2881 3543 2096
Size: 432 353 662 2824
Fragments arranged by size:
2824 662 432 353
AflIII A'CryG_T
Cuts at: 1623 3423 3704 1623
Size: 1800 281 2190
Fragments arranged by size:
2190 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 846 846
Size: 4271
AlwNI CAG_nnn'CTG
Cuts at: 693 996 1101 1576 3014 693
Size: 303 105 475 1438 1950
Fragments arranged by size:
1950 1438 475 303 105
ApaI G_GGCC'C
Cuts at: 124 124
Size: 4271
ApaLI G'TGCA_C
Cuts at: 256 3109 256
Size: 2853 1418
ApoI r'AATT_y
Cuts at: 1275 3624 3880 1275
Size: 2349 256 1666
Fragments arranged by size:
2349 1666 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4271
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4271
AvaI C'yCGr_G
Cuts at: 756 1291 756
Size: 535 3736
BamHI G'GATC_C
Cuts at: 1296 1296
Size: 4271
BanI G'GyrC_C
Cuts at: 1168 1287 1701 1949 1984 1168
Size: 119 414 248 35 3455
Fragments arranged by size:
3455 414 248 119 35
BanII G_rGCy'C
Cuts at: 124 916 1285 2315 124
Size: 792 369 1030 2080
Fragments arranged by size:
2080 1030 792 369
BbeI G_GCGC'C
Cuts at: 1705 1953 1705
Size: 248 4023
BbsI GAAGACnn'nnnn_
Cuts at: 866 866
Size: 4271
BbvCI CC'TCA_GC
Cuts at: 384 384
Size: 4271
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4237
BciVI GTATCCnnnnn_n'
Cuts at: 460 2169 3225 4271 460
Size: 1709 1056 1046 460
Fragments arranged by size:
1709 1056 1046 460
BclI T'GATC_A
Cuts at: 1791 1791
Size: 4271
BfrBI ATG'CAT
Cuts at: 111 111
Size: 4271
BglI GCCn_nnn'nGGC
Cuts at: 128 359 493 543 634 741 128
Size: 231 134 50 91 107 3658
Fragments arranged by size:
3658 231 134 107 91 50
BglII A'GATC_T
Cuts at: 1786 3823 1786
Size: 2037 2234
BlpI GC'TnA_GC
Cuts at: 583 1383 583
Size: 800 3471
Bme1580I G_kGCm'C
Cuts at: 124 260 364 534 1204 1896 1989 3113 124
Size: 136 104 170 670 692 93 1124 1282
Fragments arranged by size:
1282 1124 692 670 170 136 104 93
BmrI ACTGGGnnnn_n'
Cuts at: 864 945 1483 1564 1899 4043 864
Size: 81 538 81 335 2144 1092
Fragments arranged by size:
2144 1092 538 335 81 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 933 2638 933
Size: 1705 2566
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1123 1123
Size: 4271
Bpu10I CC'TnA_GC
Cuts at: 310 384 310
Size: 74 4197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1447 2326 3040 3338 1447
Size: 879 714 298 2380
Fragments arranged by size:
2380 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1201 3975 1201
Size: 2774 1497
BsaAI yAC'GTr
Cuts at: 1624 1624
Size: 4271
BsaBI GATnn'nnATC
Cuts at: 6 1785 3822 6
Size: 1779 2037 455
Fragments arranged by size:
2037 1779 455
BsaHI Gr'CG_yC
Cuts at: 704 1702 1950 2652 4148 704
Size: 998 248 702 1496 827
Fragments arranged by size:
1496 998 827 702 248
BsaWI w'CCGG_w
Cuts at: 656 1460 1981 2659 3070 3217 3491 656
Size: 804 521 678 411 147 274 1436
Fragments arranged by size:
1436 804 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1181 1211 1181
Size: 30 4241
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 150 906 1071 150
Size: 756 165 3350
Fragments arranged by size:
3350 756 165
BseYI C'CCAG_C
Cuts at: 141 496 646 940 1696 2454 3119 3527 141
Size: 355 150 294 756 758 665 408 885
Fragments arranged by size:
885 758 756 665 408 355 294 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 732 916 470
Size: 262 184 3825
Fragments arranged by size:
3825 262 184
BsiEI CG_ry'CG
Cuts at: 104 1813 1859 3089 104
Size: 1709 46 1230 1286
Fragments arranged by size:
1709 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 187 260 1285 2063 2253 3113 187
Size: 73 1025 778 190 860 1345
Fragments arranged by size:
1345 1025 860 778 190 73
Bsp1286I G_dGCh'C
Cuts at: 124 187 260 364 534 916 1204 1285 1896
Size: 63 73 104 170 382 288 81 611
Cuts at: 1896 1989 2063 2253 2315 3113 124
Size: 93 74 190 62 798 1282
Fragments arranged by size:
1282 798 611 382 288 190 170 104
93 81 74 73 63 62
BspHI T'CATG_A
Cuts at: 2703 2703
Size: 4271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 243 678 1321 1535 1837 2218 243
Size: 435 643 214 302 381 2296
Fragments arranged by size:
2296 643 435 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2563 2617 4101 4266 50
Size: 2513 54 1484 165 55
Fragments arranged by size:
2513 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2183 2183
Size: 4271
BsrFI r'CCGG_y
Cuts at: 205 260 454 628 2269 2450 205
Size: 55 194 174 1641 181 2026
Fragments arranged by size:
2026 1641 194 181 174 55
BssSI C'ACGA_G
Cuts at: 2542 3250 2542
Size: 708 3563
BstAPI GCAn_nnn'nTGC
Cuts at: 264 693 996 1648 264
Size: 429 303 652 2887
Fragments arranged by size:
2887 652 429 303
BstBI TT'CG_AA
Cuts at: 2632 2632
Size: 4271
BstEII G'GTnAC_C
Cuts at: 1215 1215
Size: 4271
BstYI r'GATC_y
Cuts at: 1 1296 1763 1786 2121 2367 2673 2685 2771
Size: 1295 467 23 335 246 306 12 86
Cuts at: 2771 3823 1
Size: 1052 449
Fragments arranged by size:
1295 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 739 886 739
Size: 147 4124
BtgI C'CryG_G
Cuts at: 126 525 541 553 1057 1221 126
Size: 399 16 12 504 164 3176
Fragments arranged by size:
3176 504 399 164 16 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2221 2402 2565 2221
Size: 181 163 3927
Fragments arranged by size:
3927 181 163
BtsI GCAGTG_nn'
Cuts at: 145 425 532 577 1514 145
Size: 280 107 45 937 2902
Fragments arranged by size:
2902 937 280 107 45
DraI TTT'AAA
Cuts at: 1270 1270
Size: 4271
DraIII CAC_nnn'GTG
Cuts at: 191 191
Size: 4271
DrdI GACnn_nn'nnGTC
Cuts at: 1977 3321 1977
Size: 1344 2927
EaeI y'GGCC_r
Cuts at: 129 485 1856 2030 2421 2448 3793 129
Size: 356 1371 174 391 27 1345 607
Fragments arranged by size:
1371 1345 607 391 356 174 27
EagI C'GGCC_G
Cuts at: 1856 1856
Size: 4271
EarI CTCTTCn'nnn_
Cuts at: 2294 2504 2294
Size: 210 4061
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 784 2479 3221 3367 4081 784
Size: 1695 742 146 714 974
Fragments arranged by size:
1695 974 742 714 146
EcoICRI GAG'CTC
Cuts at: 1283 1283
Size: 4271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1123 2096 2528 2881 3543 1123
Size: 973 432 353 662 1851
Fragments arranged by size:
1851 973 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 120 121 650 716 1252 1410 120
Size: 1 529 66 536 158 2981
Fragments arranged by size:
2981 536 529 158 66 1
EcoRI G'AATT_C
Cuts at: 1275 1275
Size: 4271
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 4271
FseI GG_CCGG'CC
Cuts at: 209 209
Size: 4271
FspI TGC'GCA
Cuts at: 2052 2052
Size: 4271
HaeII r_GCGC'y
Cuts at: 1705 1953 3183 3954 1705
Size: 248 1230 771 2022
Fragments arranged by size:
2022 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 832 864 2113 2143 2145 2175 832
Size: 32 1249 30 2 30 2928
Fragments arranged by size:
2928 1249 32 30 30 2
HincII GTy'rAC
Cuts at: 1310 1310
Size: 4271
KasI G'GCGC_C
Cuts at: 1701 1949 1701
Size: 248 4023
KpnI G_GTAC'C
Cuts at: 1291 1291
Size: 4271
MluI A'CGCG_T
Cuts at: 3704 3704
Size: 4271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1167 3030 3214 1167
Size: 1863 184 2224
Fragments arranged by size:
2224 1863 184
MscI TGG'CCA
Cuts at: 131 487 2032 131
Size: 356 1545 2370
Fragments arranged by size:
2370 1545 356
MslI CAynn'nnrTG
Cuts at: 2260 2387 2260
Size: 127 4144
MspA1I CmG'CkG
Cuts at: 461 646 1101 1382 1696 2056 2840 3085 461
Size: 185 455 281 314 360 784 245 1647
Fragments arranged by size:
1647 784 455 360 314 281 245 185
NaeI GCC'GGC
Cuts at: 207 456 630 2452 207
Size: 249 174 1822 2026
Fragments arranged by size:
2026 1822 249 174
NarI GG'CG_CC
Cuts at: 1702 1950 1702
Size: 248 4023
NcoI C'CATG_G
Cuts at: 126 1057 1221 126
Size: 931 164 3176
Fragments arranged by size:
3176 931 164
NgoMIV G'CCGG_C
Cuts at: 205 454 628 2450 205
Size: 249 174 1822 2026
Fragments arranged by size:
2026 1822 249 174
NsiI A_TGCA'T
Cuts at: 113 113
Size: 4271
NspI r_CATG'y
Cuts at: 115 382 474 1324 1824 3427 115
Size: 267 92 850 500 1603 959
Fragments arranged by size:
1603 959 850 500 267 92
PasI CC'CwG_GG
Cuts at: 279 958 279
Size: 679 3592
PciI A'CATG_T
Cuts at: 3423 3423
Size: 4271
PflMI CCAn_nnn'nTGG
Cuts at: 279 279
Size: 4271
PmeI GTTT'AAAC
Cuts at: 1270 1270
Size: 4271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2717 2749 2717
Size: 32 4239
PpuMI rG'GwC_Cy
Cuts at: 716 1252 716
Size: 536 3735
PspOMI G'GGCC_C
Cuts at: 120 120
Size: 4271
PstI C_TGCA'G
Cuts at: 608 689 698 1001 1097 1318 2003 608
Size: 81 9 303 96 221 685 2876
Fragments arranged by size:
2876 685 303 221 96 81 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4271
PvuII CAG'CTG
Cuts at: 461 646 1101 1696 2056 461
Size: 185 455 595 360 2676
Fragments arranged by size:
2676 595 455 360 185
RsrII CG'GwC_CG
Cuts at: 2466 2466
Size: 4271
SacI G_AGCT'C
Cuts at: 1285 1285
Size: 4271
SalI G'TCGA_C
Cuts at: 1308 1308
Size: 4271
SapI GCTCTTCn'nnn_
Cuts at: 2294 2504 2294
Size: 210 4061
SbfI CC_TGCA'GG
Cuts at: 1318 1318
Size: 4271
ScaI AGT'ACT
Cuts at: 3788 3788
Size: 4271
SfcI C'TryA_G
Cuts at: 33 604 685 694 997 1093 1314 1999 2967
Size: 571 81 9 303 96 221 685 968
Cuts at: 2967 3158 33
Size: 191 1146
Fragments arranged by size:
1146 968 685 571 303 221 191 96
81 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 128 128
Size: 4271
SfoI GGC'GCC
Cuts at: 1703 1951 1703
Size: 248 4023
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4271
SmaI CCC'GGG
Cuts at: 758 1293 758
Size: 535 3736
SmlI C'TyrA_G
Cuts at: 1426 2341 3055 3317 1426
Size: 915 714 262 2380
Fragments arranged by size:
2380 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4271
SphI G_CATG'C
Cuts at: 115 382 1324 1824 115
Size: 267 942 500 2562
Fragments arranged by size:
2562 942 500 267
StuI AGG'CCT
Cuts at: 302 434 804 891 929 1015 302
Size: 132 370 87 38 86 3558
Fragments arranged by size:
3558 370 132 87 86 38
StyI C'CwwG_G
Cuts at: 126 435 719 980 1057 1221 1405 1759 126
Size: 309 284 261 77 164 184 354 2638
Fragments arranged by size:
2638 354 309 284 261 184 164 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 799 1854 1987 2446 2655 799
Size: 1055 133 459 209 2415
Fragments arranged by size:
2415 1055 459 209 133
TatI w'GTAC_w
Cuts at: 3786 3786
Size: 4271
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1181 1213 3788 3820 1181
Size: 32 2575 32 1632
Fragments arranged by size:
2575 1632 32 32
Tth111I GACn'n_nGTC
Cuts at: 2068 2068
Size: 4271
XbaI T'CTAG_A
Cuts at: 1302 1302
Size: 4271
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1158 1228 1766 1158
Size: 70 538 3663
Fragments arranged by size:
3663 538 70
XmaI C'CCGG_G
Cuts at: 756 1291 756
Size: 535 3736
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AlfI AloI AscI
AvrII BaeI BmgBI BmtI BsiWI BsmI BsmBI BspEI
BsrGI BssHII BstXI BstZ17I ClaI CspCI EcoNI EcoRV
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI