Restriction map for
pF1KB8713
(Circular) (Six-base) MAPSORT of: pF1KB8713.seq Check: 9367 from: 1 to: 4040
pF1KB8713 4040 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 651 693 798 903 945 651
Size: 42 105 105 42 3746
Fragments arranged by size:
3746 105 105 42 42
AccI GT'mk_AC
Cuts at: 1078 1078
Size: 4040
Acc65I G'GTAC_C
Cuts at: 1056 1056
Size: 4040
AclI AA'CG_TT
Cuts at: 3880 3880
Size: 4040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 441 1865 2297 2650 3312 441
Size: 1424 432 353 662 1169
Fragments arranged by size:
1424 1169 662 432 353
AflIII A'CryG_T
Cuts at: 1392 3192 3473 1392
Size: 1800 281 1959
Fragments arranged by size:
1959 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1345 2783 1345
Size: 1438 2602
ApaLI G'TGCA_C
Cuts at: 597 2878 597
Size: 2281 1759
ApoI r'AATT_y
Cuts at: 478 566 1044 3393 3649 478
Size: 88 478 2349 256 869
Fragments arranged by size:
2349 869 478 256 88
AseI AT'TA_AT
Cuts at: 20 20
Size: 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 518 1060 518
Size: 542 3498
BamHI G'GATC_C
Cuts at: 1065 1065
Size: 4040
BanI G'GyrC_C
Cuts at: 1056 1470 1718 1753 1056
Size: 414 248 35 3343
Fragments arranged by size:
3343 414 248 35
BanII G_rGCy'C
Cuts at: 150 200 639 660 681 702 765 786 807
Size: 50 439 21 21 21 63 21 21
Cuts at: 807 828 870 891 1054 2084 150
Size: 21 42 21 163 1030 2106
Fragments arranged by size:
2106 1030 439 163 63 50 42 21
21 21 21 21 21 21
BbeI G_GCGC'C
Cuts at: 1474 1722 1474
Size: 248 3792
BbsI GAAGACnn'nnnn_
Cuts at: 447 447
Size: 4040
BciVI GTATCCnnnnn_n'
Cuts at: 664 727 769 916 1938 2994 4040 664
Size: 63 42 147 1022 1056 1046 664
Fragments arranged by size:
1056 1046 1022 664 147 63 42
BclI T'GATC_A
Cuts at: 1560 1560
Size: 4040
BglII A'GATC_T
Cuts at: 1555 3592 1555
Size: 2037 2003
BlpI GC'TnA_GC
Cuts at: 1152 1152
Size: 4040
Bme1580I G_kGCm'C
Cuts at: 601 1665 1758 2882 601
Size: 1064 93 1124 1759
Fragments arranged by size:
1759 1124 1064 93
BmrI ACTGGGnnnn_n'
Cuts at: 480 590 1252 1333 1668 3812 480
Size: 110 662 81 335 2144 708
Fragments arranged by size:
2144 708 662 335 110 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 2095 2809 3107 1216
Size: 879 714 298 2149
Fragments arranged by size:
2149 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 188 3744 188
Size: 3556 484
BsaAI yAC'GTr
Cuts at: 1393 1393
Size: 4040
BsaBI GATnn'nnATC
Cuts at: 6 1554 3591 6
Size: 1548 2037 455
Fragments arranged by size:
2037 1548 455
BsaHI Gr'CG_yC
Cuts at: 1471 1719 2421 3917 1471
Size: 248 702 1496 1594
Fragments arranged by size:
1594 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1229 1750 2428 2839 2986 3260 1229
Size: 521 678 411 147 274 2009
Fragments arranged by size:
2009 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1005 1005
Size: 4040
BseYI C'CCAG_C
Cuts at: 425 1465 2223 2888 3296 425
Size: 1040 758 665 408 1169
Fragments arranged by size:
1169 1040 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1582 1628 2858 104
Size: 1478 46 1230 1286
Fragments arranged by size:
1478 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 200 601 1054 1832 2022 2882 200
Size: 401 453 778 190 860 1358
Fragments arranged by size:
1358 860 778 453 401 190
Bsp1286I G_dGCh'C
Cuts at: 150 200 601 639 660 681 702 765 786
Size: 50 401 38 21 21 21 63 21
Cuts at: 786 807 828 870 891 1054 1665 1758 1832
Size: 21 21 42 21 163 611 93 74
Cuts at: 1832 2022 2084 2882 150
Size: 190 62 798 1308
Fragments arranged by size:
1308 798 611 401 190 163 93 74
63 62 50 42 38 21 21 21
21 21 21 21
BspHI T'CATG_A
Cuts at: 2472 2472
Size: 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 651 693 798 903 945 1090 1304 1606 1987
Size: 42 105 105 42 145 214 302 381
Cuts at: 1987 651
Size: 2704
Fragments arranged by size:
2704 381 302 214 145 105 105 42
42
BsrBI CCG'CTC
Cuts at: 50 2332 2386 3870 4035 50
Size: 2282 54 1484 165 55
Fragments arranged by size:
2282 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1952 1952
Size: 4040
BsrFI r'CCGG_y
Cuts at: 2038 2219 2038
Size: 181 3859
BssSI C'ACGA_G
Cuts at: 2311 3019 2311
Size: 708 3332
BstAPI GCAn_nnn'nTGC
Cuts at: 1417 1417
Size: 4040
BstBI TT'CG_AA
Cuts at: 2401 2401
Size: 4040
BstYI r'GATC_y
Cuts at: 1 1065 1532 1555 1890 2136 2442 2454 2540
Size: 1064 467 23 335 246 306 12 86
Cuts at: 2540 3592 1
Size: 1052 449
Fragments arranged by size:
1064 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 959 959
Size: 4040
BtgI C'CryG_G
Cuts at: 107 141 107
Size: 34 4006
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1990 2171 2334 1990
Size: 181 163 3696
Fragments arranged by size:
3696 181 163
BtsI GCAGTG_nn'
Cuts at: 1283 1283
Size: 4040
DraI TTT'AAA
Cuts at: 447 1039 447
Size: 592 3448
DrdI GACnn_nn'nnGTC
Cuts at: 1746 3090 1746
Size: 1344 2696
EaeI y'GGCC_r
Cuts at: 1625 1799 2190 2217 3562 1625
Size: 174 391 27 1345 2103
Fragments arranged by size:
2103 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1625 1625
Size: 4040
EarI CTCTTCn'nnn_
Cuts at: 1007 1023 2063 2273 1007
Size: 16 1040 210 2774
Fragments arranged by size:
2774 1040 210 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2248 2990 3136 3850 2248
Size: 742 146 714 2438
Fragments arranged by size:
2438 742 714 146
EcoICRI GAG'CTC
Cuts at: 198 1052 198
Size: 854 3186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 441 1865 2297 2650 3312 441
Size: 1424 432 353 662 1169
Fragments arranged by size:
1424 1169 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1179 1179
Size: 4040
EcoRI G'AATT_C
Cuts at: 1044 1044
Size: 4040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 4040
FspI TGC'GCA
Cuts at: 1821 1821
Size: 4040
HaeII r_GCGC'y
Cuts at: 1474 1722 2952 3723 1474
Size: 248 1230 771 1791
Fragments arranged by size:
1791 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 137 169 1882 1912 1914 1944 137
Size: 32 1713 30 2 30 2233
Fragments arranged by size:
2233 1713 32 30 30 2
HincII GTy'rAC
Cuts at: 1079 1079
Size: 4040
KasI G'GCGC_C
Cuts at: 1470 1718 1470
Size: 248 3792
KpnI G_GTAC'C
Cuts at: 1060 1060
Size: 4040
MluI A'CGCG_T
Cuts at: 3473 3473
Size: 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2799 2983 2799
Size: 184 3856
MscI TGG'CCA
Cuts at: 1801 1801
Size: 4040
MslI CAynn'nnrTG
Cuts at: 112 2029 2156 112
Size: 1917 127 1996
Fragments arranged by size:
1996 1917 127
MspA1I CmG'CkG
Cuts at: 143 1151 1465 1825 2609 2854 143
Size: 1008 314 360 784 245 1329
Fragments arranged by size:
1329 1008 784 360 314 245
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 4040
NarI GG'CG_CC
Cuts at: 1471 1719 1471
Size: 248 3792
NcoI C'CATG_G
Cuts at: 107 107
Size: 4040
NdeI CA'TA_TG
Cuts at: 417 417
Size: 4040
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 4040
NspI r_CATG'y
Cuts at: 1093 1593 3196 1093
Size: 500 1603 1937
Fragments arranged by size:
1937 1603 500
PciI A'CATG_T
Cuts at: 3192 3192
Size: 4040
PflMI CCAn_nnn'nTGG
Cuts at: 421 492 772 835 421
Size: 71 280 63 3626
Fragments arranged by size:
3626 280 71 63
PfoI T'CCnGG_A
Cuts at: 666 666
Size: 4040
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 837 869 2486 2518 837
Size: 32 1617 32 2359
Fragments arranged by size:
2359 1617 32 32
PstI C_TGCA'G
Cuts at: 648 690 795 900 1087 1772 648
Size: 42 105 105 187 685 2916
Fragments arranged by size:
2916 685 187 105 105 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4040
PvuII CAG'CTG
Cuts at: 1465 1825 1465
Size: 360 3680
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 200 1054 200
Size: 854 3186
SacII CC_GC'GG
Cuts at: 144 144
Size: 4040
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
SbfI CC_TGCA'GG
Cuts at: 648 690 795 900 1087 648
Size: 42 105 105 187 3601
Fragments arranged by size:
3601 187 105 105 42
ScaI AGT'ACT
Cuts at: 3557 3557
Size: 4040
SfcI C'TryA_G
Cuts at: 33 644 686 791 896 968 1083 1768 2736
Size: 611 42 105 105 72 115 685 968
Cuts at: 2736 2927 33
Size: 191 1146
Fragments arranged by size:
1146 968 685 611 191 115 105 105
72 42
SfoI GGC'GCC
Cuts at: 1472 1720 1472
Size: 248 3792
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SmaI CCC'GGG
Cuts at: 1062 1062
Size: 4040
SmlI C'TyrA_G
Cuts at: 1195 2110 2824 3086 1195
Size: 915 714 262 2149
Fragments arranged by size:
2149 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4040
SphI G_CATG'C
Cuts at: 1093 1593 1093
Size: 500 3540
StuI AGG'CCT
Cuts at: 957 957
Size: 4040
StyI C'CwwG_G
Cuts at: 107 1174 1528 107
Size: 1067 354 2619
Fragments arranged by size:
2619 1067 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1623 1756 2215 2424 1623
Size: 133 459 209 3239
Fragments arranged by size:
3239 459 209 133
TatI w'GTAC_w
Cuts at: 3555 3555
Size: 4040
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3557 3589 3557
Size: 32 4008
Tth111I GACn'n_nGTC
Cuts at: 1837 1837
Size: 4040
XbaI T'CTAG_A
Cuts at: 1071 1071
Size: 4040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 422 1535 422
Size: 1113 2927
XmaI C'CCGG_G
Cuts at: 1060 1060
Size: 4040
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PasI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI