Restriction map for
pF1KB8711
(Circular) (Six-base) MAPSORT of: pF1KB8711.seq Check: 7487 from: 1 to: 4559
pF1KB8711 4559 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AccI GT'mk_AC
Cuts at: 1597 1597
Size: 4559
Acc65I G'GTAC_C
Cuts at: 1575 1575
Size: 4559
AclI AA'CG_TT
Cuts at: 4399 4399
Size: 4559
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1082 1220 2384 2816 3563 3831 1082
Size: 138 1164 432 747 268 1810
Fragments arranged by size:
1810 1164 747 432 268 138
AflIII A'CryG_T
Cuts at: 246 629 1911 3015 3992 246
Size: 383 1282 1104 977 813
Fragments arranged by size:
1282 1104 977 813 383
AgeI A'CCGG_T
Cuts at: 2947 2947
Size: 4559
AlwNI CAG_nnn'CTG
Cuts at: 1864 3431 1864
Size: 1567 2992
ApaLI G'TGCA_C
Cuts at: 3329 3329
Size: 4559
ApoI r'AATT_y
Cuts at: 391 892 1563 3912 4168 391
Size: 501 671 2349 256 782
Fragments arranged by size:
2349 782 671 501 256
AseI AT'TA_AT
Cuts at: 20 1009 20
Size: 989 3570
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
AvaI C'yCGr_G
Cuts at: 1579 1579
Size: 4559
BamHI G'GATC_C
Cuts at: 1584 1584
Size: 4559
BanI G'GyrC_C
Cuts at: 1575 1989 2237 2272 1575
Size: 414 248 35 3862
Fragments arranged by size:
3862 414 248 35
BanII G_rGCy'C
Cuts at: 1573 2603 1573
Size: 1030 3529
BbeI G_GCGC'C
Cuts at: 1993 2241 1993
Size: 248 4311
BciVI GTATCCnnnnn_n'
Cuts at: 2457 3218 4559 2457
Size: 761 1341 2457
Fragments arranged by size:
2457 1341 761
BclI T'GATC_A
Cuts at: 2079 2079
Size: 4559
BglII A'GATC_T
Cuts at: 1238 2074 4111 1238
Size: 836 2037 1686
Fragments arranged by size:
2037 1686 836
BlpI GC'TnA_GC
Cuts at: 1671 1671
Size: 4559
Bme1580I G_kGCm'C
Cuts at: 1328 2184 2277 3333 1328
Size: 856 93 1056 2554
Fragments arranged by size:
2554 1056 856 93
BmgBI CAC'GTC
Cuts at: 630 630
Size: 4559
BmrI ACTGGGnnnn_n'
Cuts at: 394 1771 1852 2187 4331 394
Size: 1377 81 335 2144 622
Fragments arranged by size:
2144 1377 622 335 81
BmtI G_CTAG'C
Cuts at: 799 799
Size: 4559
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2926 2926
Size: 4559
Bpu10I CC'TnA_GC
Cuts at: 1119 1371 1442 1119
Size: 252 71 4236
Fragments arranged by size:
4236 252 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 353 1735 2614 3106 3404 140
Size: 213 1382 879 492 298 1295
Fragments arranged by size:
1382 1295 879 492 298 213
BsaI GGTCTCn'nnnn_
Cuts at: 4263 4263
Size: 4559
BsaAI yAC'GTr
Cuts at: 1912 1912
Size: 4559
BsaBI GATnn'nnATC
Cuts at: 6 2073 4110 6
Size: 2067 2037 455
Fragments arranged by size:
2067 2037 455
BsaHI Gr'CG_yC
Cuts at: 1990 2238 2940 4436 1990
Size: 248 702 1496 2113
Fragments arranged by size:
2113 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1748 2269 2947 3221 3368 3779 1748
Size: 521 678 274 147 411 2528
Fragments arranged by size:
2528 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 620 620
Size: 4559
BseYI C'CCAG_C
Cuts at: 476 1984 2742 3319 3815 476
Size: 1508 758 577 496 1220
Fragments arranged by size:
1508 1220 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 609 1012 609
Size: 403 4156
BsiEI CG_ry'CG
Cuts at: 104 2101 2147 3355 104
Size: 1997 46 1208 1308
Fragments arranged by size:
1997 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 612 1573 2351 2541 3333 612
Size: 961 778 190 792 1838
Fragments arranged by size:
1838 961 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 193 1295 193
Size: 1102 3457
Bsp1286I G_dGCh'C
Cuts at: 612 1328 1573 2184 2277 2351 2541 2603 3333
Size: 716 245 611 93 74 190 62 730
Cuts at: 3333 612
Size: 1838
Fragments arranged by size:
1838 730 716 611 245 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3779 3779
Size: 4559
BspHI T'CATG_A
Cuts at: 3735 3735
Size: 4559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1229 1446 1609 1823 2125 2506 1229
Size: 217 163 214 302 381 3282
Fragments arranged by size:
3282 381 302 217 214 163
BsrBI CCG'CTC
Cuts at: 50 2851 2905 4389 4554 50
Size: 2801 54 1484 165 55
Fragments arranged by size:
2801 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2471 2471
Size: 4559
BsrFI r'CCGG_y
Cuts at: 2557 2738 2947 2557
Size: 181 209 4169
Fragments arranged by size:
4169 209 181
BssSI C'ACGA_G
Cuts at: 2830 3188 2830
Size: 358 4201
BstAPI GCAn_nnn'nTGC
Cuts at: 1936 1936
Size: 4559
BstBI TT'CG_AA
Cuts at: 2920 2920
Size: 4559
BstYI r'GATC_y
Cuts at: 1 753 1238 1584 2051 2074 2409 2655 3667
Size: 752 485 346 467 23 335 246 1012
Cuts at: 3667 3753 3765 4111 1
Size: 86 12 346 449
Fragments arranged by size:
1012 752 485 467 449 346 346 335
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2509 2690 2853 2509
Size: 181 163 4215
Fragments arranged by size:
4215 181 163
BtsI GCAGTG_nn'
Cuts at: 1802 1802
Size: 4559
ClaI AT'CG_AT
Cuts at: 147 147
Size: 4559
DraI TTT'AAA
Cuts at: 905 1558 905
Size: 653 3906
DrdI GACnn_nn'nnGTC
Cuts at: 2265 3123 2265
Size: 858 3701
EaeI y'GGCC_r
Cuts at: 1305 2144 2318 2709 2736 4081 1305
Size: 839 174 391 27 1345 1783
Fragments arranged by size:
1783 1345 839 391 174 27
EagI C'GGCC_G
Cuts at: 2144 2144
Size: 4559
EarI CTCTTCn'nnn_
Cuts at: 941 1256 1483 2582 2792 941
Size: 315 227 1099 210 2708
Fragments arranged by size:
2708 1099 315 227 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2767 3077 3223 4369 2767
Size: 310 146 1146 2957
Fragments arranged by size:
2957 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1571 1571
Size: 4559
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1082 1220 2384 2816 3563 3831 1082
Size: 138 1164 432 747 268 1810
Fragments arranged by size:
1810 1164 747 432 268 138
EcoO109I rG'GnC_Cy
Cuts at: 459 1123 1187 1698 459
Size: 664 64 511 3320
Fragments arranged by size:
3320 664 511 64
EcoRI G'AATT_C
Cuts at: 1563 1563
Size: 4559
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 228 718 772 2578 228
Size: 490 54 1806 2209
Fragments arranged by size:
2209 1806 490 54
FspI TGC'GCA
Cuts at: 2340 2340
Size: 4559
HaeII r_GCGC'y
Cuts at: 1993 2241 3263 4242 1993
Size: 248 1022 979 2310
Fragments arranged by size:
2310 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 297 329 764 796 1084 1116 1135 1167 1483
Size: 32 435 32 288 32 19 32 316
Cuts at: 1483 1515 2401 2431 2433 2463 297
Size: 32 886 30 2 30 2393
Fragments arranged by size:
2393 886 435 316 288 32 32 32
32 32 30 30 19 2
HincII GTy'rAC
Cuts at: 1598 1598
Size: 4559
KasI G'GCGC_C
Cuts at: 1989 2237 1989
Size: 248 4311
KpnI G_GTAC'C
Cuts at: 1579 1579
Size: 4559
MfeI C'AATT_G
Cuts at: 332 332
Size: 4559
MluI A'CGCG_T
Cuts at: 3992 3992
Size: 4559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3230 3414 3230
Size: 184 4375
MscI TGG'CCA
Cuts at: 2320 2320
Size: 4559
MslI CAynn'nnrTG
Cuts at: 615 2548 2675 615
Size: 1933 127 2499
Fragments arranged by size:
2499 1933 127
MspA1I CmG'CkG
Cuts at: 1291 1670 1984 2344 3357 3602 1291
Size: 379 314 360 1013 245 2248
Fragments arranged by size:
2248 1013 379 360 314 245
NaeI GCC'GGC
Cuts at: 2740 2740
Size: 4559
NarI GG'CG_CC
Cuts at: 1990 2238 1990
Size: 248 4311
NdeI CA'TA_TG
Cuts at: 888 888
Size: 4559
NgoMIV G'CCGG_C
Cuts at: 2738 2738
Size: 4559
NheI G'CTAG_C
Cuts at: 795 795
Size: 4559
NspI r_CATG'y
Cuts at: 250 795 1118 1612 2112 3019 250
Size: 545 323 494 500 907 1790
Fragments arranged by size:
1790 907 545 500 494 323
PasI CC'CwG_GG
Cuts at: 131 1338 131
Size: 1207 3352
PciI A'CATG_T
Cuts at: 246 3015 246
Size: 2769 1790
PflMI CCAn_nnn'nTGG
Cuts at: 525 525
Size: 4559
PmeI GTTT'AAAC
Cuts at: 1558 1558
Size: 4559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1255 1287 3698 3730 1255
Size: 32 2411 32 2084
Fragments arranged by size:
2411 2084 32 32
PpuMI rG'GwC_Cy
Cuts at: 1123 1187 1123
Size: 64 4495
PstI C_TGCA'G
Cuts at: 1606 2291 1606
Size: 685 3874
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4559
PvuII CAG'CTG
Cuts at: 1984 2344 1984
Size: 360 4199
RsrII CG'GwC_CG
Cuts at: 2754 2754
Size: 4559
SacI G_AGCT'C
Cuts at: 1573 1573
Size: 4559
SalI G'TCGA_C
Cuts at: 1596 1596
Size: 4559
SanDI GG'GwC_CC
Cuts at: 1187 1187
Size: 4559
SapI GCTCTTCn'nnn_
Cuts at: 2582 2792 2582
Size: 210 4349
SbfI CC_TGCA'GG
Cuts at: 1606 1606
Size: 4559
ScaI AGT'ACT
Cuts at: 4076 4076
Size: 4559
SfcI C'TryA_G
Cuts at: 33 1602 2287 3280 3471 33
Size: 1569 685 993 191 1121
Fragments arranged by size:
1569 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1991 2239 1991
Size: 248 4311
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
SmaI CCC'GGG
Cuts at: 1581 1581
Size: 4559
SmlI C'TyrA_G
Cuts at: 155 368 1714 2629 3121 3383 155
Size: 213 1346 915 492 262 1331
Fragments arranged by size:
1346 1331 915 492 262 213
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4559
SphI G_CATG'C
Cuts at: 1118 1612 2112 1118
Size: 494 500 3565
Fragments arranged by size:
3565 500 494
SspI AAT'ATT
Cuts at: 193 193
Size: 4559
StuI AGG'CCT
Cuts at: 211 1423 211
Size: 1212 3347
StyI C'CwwG_G
Cuts at: 405 1256 1355 1693 2047 405
Size: 851 99 338 354 2917
Fragments arranged by size:
2917 851 354 338 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2142 2275 2734 2943 2142
Size: 133 459 209 3758
Fragments arranged by size:
3758 459 209 133
TatI w'GTAC_w
Cuts at: 336 976 4074 336
Size: 640 3098 821
Fragments arranged by size:
3098 821 640
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4076 4108 4076
Size: 32 4527
Tth111I GACn'n_nGTC
Cuts at: 2356 2356
Size: 4559
XbaI T'CTAG_A
Cuts at: 1590 1590
Size: 4559
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1335 2054 1335
Size: 719 3840
XmaI C'CCGG_G
Cuts at: 1579 1579
Size: 4559
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NheI
NspI PasI PciI PflMI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BpmI BsaXI BsiWI BsmI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NcoI NotI NruI NsiI
PacI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
XmnI ZraI