Restriction map for
pF1KB8703
(Circular) (Six-base) MAPSORT of: pF1KB8703.seq Check: 1798 from: 1 to: 4835
pF1KB8703 4835 bp
With 182 enzymes: SgfI *
February 20, 2009 16:37 ..
AccI GT'mk_AC
Cuts at: 1873 1873
Size: 4835
Acc65I G'GTAC_C
Cuts at: 1851 1851
Size: 4835
AclI AA'CG_TT
Cuts at: 4675 4675
Size: 4835
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2660 3092 3445 4107 2660
Size: 432 353 662 3388
Fragments arranged by size:
3388 662 432 353
AflIII A'CryG_T
Cuts at: 1331 2187 3987 4268 1331
Size: 856 1800 281 1898
Fragments arranged by size:
1898 1800 856 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 644 644
Size: 4835
AlwNI CAG_nnn'CTG
Cuts at: 262 1363 1700 2140 3578 262
Size: 1101 337 440 1438 1519
Fragments arranged by size:
1519 1438 1101 440 337
ApaI G_GGCC'C
Cuts at: 764 764
Size: 4835
ApaLI G'TGCA_C
Cuts at: 3673 3673
Size: 4835
ApoI r'AATT_y
Cuts at: 1075 1839 4188 4444 1075
Size: 764 2349 256 1466
Fragments arranged by size:
2349 1466 764 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4835
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4835
AvaI C'yCGr_G
Cuts at: 674 819 1855 674
Size: 145 1036 3654
Fragments arranged by size:
3654 1036 145
BamHI G'GATC_C
Cuts at: 1860 1860
Size: 4835
BanI G'GyrC_C
Cuts at: 563 642 891 1851 2265 2513 2548 563
Size: 79 249 960 414 248 35 2850
Fragments arranged by size:
2850 960 414 249 248 79 35
BanII G_rGCy'C
Cuts at: 764 970 1849 2879 764
Size: 206 879 1030 2720
Fragments arranged by size:
2720 1030 879 206
BbeI G_GCGC'C
Cuts at: 567 2269 2517 567
Size: 1702 248 2885
Fragments arranged by size:
2885 1702 248
BbsI GAAGACnn'nnnn_
Cuts at: 193 193
Size: 4835
BbvCI CC'TCA_GC
Cuts at: 324 722 1108 324
Size: 398 386 4051
Fragments arranged by size:
4051 398 386
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 452 486 452
Size: 34 4801
BciVI GTATCCnnnnn_n'
Cuts at: 2733 3789 4835 2733
Size: 1056 1046 2733
Fragments arranged by size:
2733 1056 1046
BclI T'GATC_A
Cuts at: 2355 2355
Size: 4835
BglI GCCn_nnn'nGGC
Cuts at: 572 759 572
Size: 187 4648
BglII A'GATC_T
Cuts at: 1793 2350 4387 1793
Size: 557 2037 2241
Fragments arranged by size:
2241 2037 557
BlpI GC'TnA_GC
Cuts at: 1947 1947
Size: 4835
Bme1580I G_kGCm'C
Cuts at: 755 764 2460 2553 3677 755
Size: 9 1696 93 1124 1913
Fragments arranged by size:
1913 1696 1124 93 9
BmrI ACTGGGnnnn_n'
Cuts at: 2047 2128 2463 4607 2047
Size: 81 335 2144 2275
Fragments arranged by size:
2275 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 758 3202 758
Size: 2444 2391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1156 1513 1156
Size: 357 4478
Bpu10I CC'TnA_GC
Cuts at: 146 324 722 1108 1583 146
Size: 178 398 386 475 3398
Fragments arranged by size:
3398 475 398 386 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 217 2011 2890 3604 3902 165
Size: 52 1794 879 714 298 1098
Fragments arranged by size:
1794 1098 879 714 298 52
BsaI GGTCTCn'nnnn_
Cuts at: 746 1334 4539 746
Size: 588 3205 1042
Fragments arranged by size:
3205 1042 588
BsaAI yAC'GTr
Cuts at: 1266 2188 1266
Size: 922 3913
BsaBI GATnn'nnATC
Cuts at: 6 2349 4386 6
Size: 2343 2037 455
Fragments arranged by size:
2343 2037 455
BsaHI Gr'CG_yC
Cuts at: 291 564 634 2266 2514 3216 4712 291
Size: 273 70 1632 248 702 1496 414
Fragments arranged by size:
1632 1496 702 414 273 248 70
BsaWI w'CCGG_w
Cuts at: 506 2024 2545 3223 3634 3781 4055 506
Size: 1518 521 678 411 147 274 1286
Fragments arranged by size:
1518 1286 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1331 1361 1331
Size: 30 4805
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 352 364 367 370 391 794 1054 352
Size: 12 3 3 21 403 260 4133
Fragments arranged by size:
4133 403 260 21 12 3 3
BseYI C'CCAG_C
Cuts at: 171 420 431 617 696 1763 2260 3018 3683
Size: 249 11 186 79 1067 497 758 665
Cuts at: 3683 4091 171
Size: 408 915
Fragments arranged by size:
1067 915 758 665 497 408 249 186
79 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 948 948
Size: 4835
BsiEI CG_ry'CG
Cuts at: 104 2377 2423 3653 104
Size: 2273 46 1230 1286
Fragments arranged by size:
2273 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 714 1849 2627 2817 3677 714
Size: 1135 778 190 860 1872
Fragments arranged by size:
1872 1135 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 714 755 764 970 1849 2460 2553 2627 2817
Size: 41 9 206 879 611 93 74 190
Cuts at: 2817 2879 3677 714
Size: 62 798 1872
Fragments arranged by size:
1872 879 798 611 206 190 93 74
62 41 9
BspEI T'CCGG_A
Cuts at: 506 506
Size: 4835
BspHI T'CATG_A
Cuts at: 3267 3267
Size: 4835
BspMI ACCTGCnnnn'nnnn_
Cuts at: 478 1060 1885 2099 2401 2782 478
Size: 582 825 214 302 381 2531
Fragments arranged by size:
2531 825 582 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3127 3181 4665 4830 50
Size: 3077 54 1484 165 55
Fragments arranged by size:
3077 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 927 1230 2747 927
Size: 303 1517 3015
Fragments arranged by size:
3015 1517 303
BsrFI r'CCGG_y
Cuts at: 575 1015 2833 3014 575
Size: 440 1818 181 2396
Fragments arranged by size:
2396 1818 440 181
BssSI C'ACGA_G
Cuts at: 3106 3814 3106
Size: 708 4127
BstAPI GCAn_nnn'nTGC
Cuts at: 926 1773 2212 926
Size: 847 439 3549
Fragments arranged by size:
3549 847 439
BstBI TT'CG_AA
Cuts at: 3196 3196
Size: 4835
BstXI CCAn_nnnn'nTGG
Cuts at: 1449 1491 1449
Size: 42 4793
BstYI r'GATC_y
Cuts at: 1 258 1793 1860 2327 2350 2685 2931 3237
Size: 257 1535 67 467 23 335 246 306
Cuts at: 3237 3249 3335 4387 1
Size: 12 86 1052 449
Fragments arranged by size:
1535 1052 467 449 335 306 257 246
86 67 23 12
BtgI C'CryG_G
Cuts at: 767 1229 1274 767
Size: 462 45 4328
Fragments arranged by size:
4328 462 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2785 2966 3129 2785
Size: 181 163 4491
Fragments arranged by size:
4491 181 163
BtsI GCAGTG_nn'
Cuts at: 1812 2078 1812
Size: 266 4569
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 222 257 222
Size: 35 4800
DraI TTT'AAA
Cuts at: 1834 1834
Size: 4835
DrdI GACnn_nn'nnGTC
Cuts at: 798 2541 3885 798
Size: 1743 1344 1748
Fragments arranged by size:
1748 1743 1344
EaeI y'GGCC_r
Cuts at: 1017 1676 2420 2594 2985 3012 4357 1017
Size: 659 744 174 391 27 1345 1495
Fragments arranged by size:
1495 1345 744 659 391 174 27
EagI C'GGCC_G
Cuts at: 2420 2420
Size: 4835
EarI CTCTTCn'nnn_
Cuts at: 329 341 368 983 2858 3068 329
Size: 12 27 615 1875 210 2096
Fragments arranged by size:
2096 1875 615 210 27 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3043 3785 3931 4645 3043
Size: 742 146 714 3233
Fragments arranged by size:
3233 742 714 146
EcoICRI GAG'CTC
Cuts at: 1847 1847
Size: 4835
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1156 1513 2660 3092 3445 4107 1156
Size: 357 1147 432 353 662 1884
Fragments arranged by size:
1884 1147 662 432 357 353
EcoNI CCTnn'n_nnAGG
Cuts at: 266 1036 266
Size: 770 4065
EcoO109I rG'GnC_Cy
Cuts at: 134 142 585 761 1179 1289 1974 134
Size: 8 443 176 418 110 685 2995
Fragments arranged by size:
2995 685 443 418 176 110 8
EcoRI G'AATT_C
Cuts at: 1075 1839 1075
Size: 764 4071
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2854 2854
Size: 4835
FspI TGC'GCA
Cuts at: 2616 2616
Size: 4835
HaeII r_GCGC'y
Cuts at: 483 567 2269 2517 3747 4518 483
Size: 84 1702 248 1230 771 800
Fragments arranged by size:
1702 1230 800 771 248 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 250 282 802 834 1331 1363 1523 1555 2677
Size: 32 520 32 497 32 160 32 1122
Cuts at: 2677 2707 2709 2739 250
Size: 30 2 30 2346
Fragments arranged by size:
2346 1122 520 497 160 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1874 1874
Size: 4835
HindIII A'AGCT_T
Cuts at: 553 553
Size: 4835
KasI G'GCGC_C
Cuts at: 563 2265 2513 563
Size: 1702 248 2885
Fragments arranged by size:
2885 1702 248
KpnI G_GTAC'C
Cuts at: 1855 1855
Size: 4835
MluI A'CGCG_T
Cuts at: 4268 4268
Size: 4835
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3594 3778 3594
Size: 184 4651
MscI TGG'CCA
Cuts at: 1678 2596 1678
Size: 918 3917
MslI CAynn'nnrTG
Cuts at: 2824 2951 2824
Size: 127 4708
MspA1I CmG'CkG
Cuts at: 420 435 1194 1212 1577 1946 2260 2620 3404
Size: 15 759 18 365 369 314 360 784
Cuts at: 3404 3649 420
Size: 245 1606
Fragments arranged by size:
1606 784 759 369 365 360 314 245
18 15
NaeI GCC'GGC
Cuts at: 577 1017 3016 577
Size: 440 1999 2396
Fragments arranged by size:
2396 1999 440
NarI GG'CG_CC
Cuts at: 564 2266 2514 564
Size: 1702 248 2885
Fragments arranged by size:
2885 1702 248
NcoI C'CATG_G
Cuts at: 767 1229 1274 767
Size: 462 45 4328
Fragments arranged by size:
4328 462 45
NgoMIV G'CCGG_C
Cuts at: 575 1015 3014 575
Size: 440 1999 2396
Fragments arranged by size:
2396 1999 440
NspI r_CATG'y
Cuts at: 1048 1335 1776 1888 2388 3991 1048
Size: 287 441 112 500 1603 1892
Fragments arranged by size:
1892 1603 500 441 287 112
PciI A'CATG_T
Cuts at: 1331 3987 1331
Size: 2656 2179
PflMI CCAn_nnn'nTGG
Cuts at: 262 262
Size: 4835
PfoI T'CCnGG_A
Cuts at: 153 1171 153
Size: 1018 3817
PmeI GTTT'AAAC
Cuts at: 1834 1834
Size: 4835
PmlI CAC'GTG
Cuts at: 1266 1266
Size: 4835
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3281 3313 3281
Size: 32 4803
PpuMI rG'GwC_Cy
Cuts at: 134 1289 134
Size: 1155 3680
PshAI GACnn'nnGTC
Cuts at: 234 234
Size: 4835
PsiI TTA'TAA
Cuts at: 1256 1256
Size: 4835
PspOMI G'GGCC_C
Cuts at: 760 760
Size: 4835
PstI C_TGCA'G
Cuts at: 1882 2567 1882
Size: 685 4150
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4835
PvuII CAG'CTG
Cuts at: 420 1194 2260 2620 420
Size: 774 1066 360 2635
Fragments arranged by size:
2635 1066 774 360
RsrII CG'GwC_CG
Cuts at: 3030 3030
Size: 4835
SacI G_AGCT'C
Cuts at: 1849 1849
Size: 4835
SalI G'TCGA_C
Cuts at: 1872 1872
Size: 4835
SapI GCTCTTCn'nnn_
Cuts at: 2858 3068 2858
Size: 210 4625
SbfI CC_TGCA'GG
Cuts at: 1882 1882
Size: 4835
ScaI AGT'ACT
Cuts at: 1723 4352 1723
Size: 2629 2206
SexAI A'CCwGG_T
Cuts at: 1319 1343 1319
Size: 24 4811
SfcI C'TryA_G
Cuts at: 33 1603 1878 2563 3531 3722 33
Size: 1570 275 685 968 191 1146
Fragments arranged by size:
1570 1146 968 685 275 191
SfoI GGC'GCC
Cuts at: 565 2267 2515 565
Size: 1702 248 2885
Fragments arranged by size:
2885 1702 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4835
SmaI CCC'GGG
Cuts at: 676 1857 676
Size: 1181 3654
SmlI C'TyrA_G
Cuts at: 180 232 1990 2905 3619 3881 180
Size: 52 1758 915 714 262 1134
Fragments arranged by size:
1758 1134 915 714 262 52
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4835
SphI G_CATG'C
Cuts at: 1888 2388 1888
Size: 500 4335
StuI AGG'CCT
Cuts at: 1002 1002
Size: 4835
StyI C'CwwG_G
Cuts at: 767 1229 1274 1412 1969 2323 767
Size: 462 45 138 557 354 3279
Fragments arranged by size:
3279 557 462 354 138 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1643 1777 2418 2551 3010 3219 1643
Size: 134 641 133 459 209 3259
Fragments arranged by size:
3259 641 459 209 134 133
TatI w'GTAC_w
Cuts at: 835 1721 4350 835
Size: 886 2629 1320
Fragments arranged by size:
2629 1320 886
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4352 4384 4352
Size: 32 4803
Tth111I GACn'n_nGTC
Cuts at: 132 1345 1357 2632 132
Size: 1213 12 1275 2335
Fragments arranged by size:
2335 1275 1213 12
XbaI T'CTAG_A
Cuts at: 1866 1866
Size: 4835
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1179 1281 2330 1179
Size: 102 1049 3684
Fragments arranged by size:
3684 1049 102
XmaI C'CCGG_G
Cuts at: 674 1855 674
Size: 1181 3654
XmnI GAAnn'nnTTC
Cuts at: 1079 1079
Size: 4835
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
AscI AvrII BaeI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI