Restriction map for
pF1KB8702
(Circular) (Six-base) MAPSORT of: pF1KB8702.seq Check: 3585 from: 1 to: 4226
pF1KB8702 4226 bp
With 182 enzymes: SgfI *
February 20, 2009 16:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1119 1119
Size: 4226
AccI GT'mk_AC
Cuts at: 1264 1264
Size: 4226
Acc65I G'GTAC_C
Cuts at: 1242 1242
Size: 4226
AclI AA'CG_TT
Cuts at: 4066 4066
Size: 4226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 2051 2483 2836 3498 226
Size: 1825 432 353 662 954
Fragments arranged by size:
1825 954 662 432 353
AflIII A'CryG_T
Cuts at: 1578 3378 3659 1578
Size: 1800 281 2145
Fragments arranged by size:
2145 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1216 1216
Size: 4226
AlwNI CAG_nnn'CTG
Cuts at: 121 429 684 735 771 990 1531 2969 121
Size: 308 255 51 36 219 541 1438 1378
Fragments arranged by size:
1438 1378 541 308 255 219 51 36
ApaI G_GGCC'C
Cuts at: 359 809 359
Size: 450 3776
ApaLI G'TGCA_C
Cuts at: 3064 3064
Size: 4226
ApoI r'AATT_y
Cuts at: 1230 3579 3835 1230
Size: 2349 256 1621
Fragments arranged by size:
2349 1621 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4226
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
AvaI C'yCGr_G
Cuts at: 150 157 358 505 544 802 808 1051 1246
Size: 7 201 147 39 258 6 243 195
Cuts at: 1246 150
Size: 3130
Fragments arranged by size:
3130 258 243 201 195 147 39 7
6
BamHI G'GATC_C
Cuts at: 1251 1251
Size: 4226
BanI G'GyrC_C
Cuts at: 142 311 484 703 792 864 886 1242 1656
Size: 169 173 219 89 72 22 356 414
Cuts at: 1656 1904 1939 142
Size: 248 35 2429
Fragments arranged by size:
2429 414 356 248 219 173 169 89
72 35 22
BanII G_rGCy'C
Cuts at: 359 809 1061 1240 2270 359
Size: 450 252 179 1030 2315
Fragments arranged by size:
2315 1030 450 252 179
BbeI G_GCGC'C
Cuts at: 146 488 707 868 890 1660 1908 146
Size: 342 219 161 22 770 248 2464
Fragments arranged by size:
2464 770 342 248 219 161 22
BbsI GAAGACnn'nnnn_
Cuts at: 708 708
Size: 4226
BciVI GTATCCnnnnn_n'
Cuts at: 2124 3180 4226 2124
Size: 1056 1046 2124
Fragments arranged by size:
2124 1056 1046
BclI T'GATC_A
Cuts at: 1746 1746
Size: 4226
BglI GCCn_nnn'nGGC
Cuts at: 112 134 829 112
Size: 22 695 3509
Fragments arranged by size:
3509 695 22
BglII A'GATC_T
Cuts at: 1741 3778 1741
Size: 2037 2189
BlpI GC'TnA_GC
Cuts at: 1157 1338 1157
Size: 181 4045
Bme1580I G_kGCm'C
Cuts at: 359 809 1851 1944 3068 359
Size: 450 1042 93 1124 1517
Fragments arranged by size:
1517 1124 1042 450 93
BmgBI CAC'GTC
Cuts at: 900 900
Size: 4226
BmrI ACTGGGnnnn_n'
Cuts at: 1438 1519 1854 3998 1438
Size: 81 335 2144 1666
Fragments arranged by size:
2144 1666 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 687 1017 2593 687
Size: 330 1576 2320
Fragments arranged by size:
2320 1576 330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 1012 1057 604
Size: 408 45 3773
Fragments arranged by size:
3773 408 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1402 2281 2995 3293 1402
Size: 879 714 298 2335
Fragments arranged by size:
2335 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3930 3930
Size: 4226
BsaAI yAC'GTr
Cuts at: 1579 1579
Size: 4226
BsaBI GATnn'nnATC
Cuts at: 6 1740 3777 6
Size: 1734 2037 455
Fragments arranged by size:
2037 1734 455
BsaHI Gr'CG_yC
Cuts at: 143 485 704 817 865 887 1657 1905 2607
Size: 342 219 113 48 22 770 248 702
Cuts at: 2607 4103 143
Size: 1496 266
Fragments arranged by size:
1496 770 702 342 266 248 219 113
48 22
BsaWI w'CCGG_w
Cuts at: 1415 1936 2614 3025 3172 3446 1415
Size: 521 678 411 147 274 2195
Fragments arranged by size:
2195 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 931 1069 931
Size: 138 4088
BseYI C'CCAG_C
Cuts at: 264 510 614 629 797 1651 2409 3074 3482
Size: 246 104 15 168 854 758 665 408
Cuts at: 3482 264
Size: 1008
Fragments arranged by size:
1008 854 758 665 408 246 168 104
15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 784 484
Size: 300 3926
BsiEI CG_ry'CG
Cuts at: 104 524 584 833 1768 1814 3044 104
Size: 420 60 249 935 46 1230 1286
Fragments arranged by size:
1286 1230 935 420 249 60 46
BsiHKAI G_wGCw'C
Cuts at: 749 980 1061 1240 2018 2208 3068 749
Size: 231 81 179 778 190 860 1907
Fragments arranged by size:
1907 860 778 231 190 179 81
Bsp1286I G_dGCh'C
Cuts at: 359 749 809 980 1061 1240 1851 1944 2018
Size: 390 60 171 81 179 611 93 74
Cuts at: 2018 2208 2270 3068 359
Size: 190 62 798 1517
Fragments arranged by size:
1517 798 611 390 190 179 171 93
81 74 62 60
BspHI T'CATG_A
Cuts at: 2658 2658
Size: 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1119 1276 1490 1792 2173 1119
Size: 157 214 302 381 3172
Fragments arranged by size:
3172 381 302 214 157
BsrBI CCG'CTC
Cuts at: 50 2518 2572 4056 4221 50
Size: 2468 54 1484 165 55
Fragments arranged by size:
2468 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2138 2138
Size: 4226
BsrFI r'CCGG_y
Cuts at: 145 185 244 519 579 592 700 789 828
Size: 40 59 275 60 13 108 89 39
Cuts at: 828 2224 2405 145
Size: 1396 181 1966
Fragments arranged by size:
1966 1396 275 181 108 89 60 59
40 39 13
BssHII G'CGCG_C
Cuts at: 279 328 418 606 688 279
Size: 49 90 188 82 3817
Fragments arranged by size:
3817 188 90 82 49
BssSI C'ACGA_G
Cuts at: 2497 3205 2497
Size: 708 3518
BstAPI GCAn_nnn'nTGC
Cuts at: 121 771 1603 121
Size: 650 832 2744
Fragments arranged by size:
2744 832 650
BstBI TT'CG_AA
Cuts at: 2587 2587
Size: 4226
BstXI CCAn_nnnn'nTGG
Cuts at: 480 480
Size: 4226
BstYI r'GATC_y
Cuts at: 1 1251 1718 1741 2076 2322 2628 2640 2726
Size: 1250 467 23 335 246 306 12 86
Cuts at: 2726 3778 1
Size: 1052 449
Fragments arranged by size:
1250 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1206 1206
Size: 4226
BtgI C'CryG_G
Cuts at: 107 351 488 1023 107
Size: 244 137 535 3310
Fragments arranged by size:
3310 535 244 137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2176 2357 2520 2176
Size: 181 163 3882
Fragments arranged by size:
3882 181 163
BtsI GCAGTG_nn'
Cuts at: 769 1469 769
Size: 700 3526
DraI TTT'AAA
Cuts at: 1225 1225
Size: 4226
DraIII CAC_nnn'GTG
Cuts at: 762 762
Size: 4226
DrdI GACnn_nn'nnGTC
Cuts at: 904 1932 3276 904
Size: 1028 1344 1854
Fragments arranged by size:
1854 1344 1028
EaeI y'GGCC_r
Cuts at: 521 551 581 641 830 1041 1141 1811 1985
Size: 30 30 60 189 211 100 670 174
Cuts at: 1985 2376 2403 3748 521
Size: 391 27 1345 999
Fragments arranged by size:
1345 999 670 391 211 189 174 100
60 30 30 27
EagI C'GGCC_G
Cuts at: 521 581 830 1811 521
Size: 60 249 981 2936
Fragments arranged by size:
2936 981 249 60
EarI CTCTTCn'nnn_
Cuts at: 352 400 2249 2459 352
Size: 48 1849 210 2119
Fragments arranged by size:
2119 1849 210 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 253 2434 3176 3322 4036 253
Size: 2181 742 146 714 443
Fragments arranged by size:
2181 742 714 443 146
EcoICRI GAG'CTC
Cuts at: 1059 1238 1059
Size: 179 4047
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 604 1012 1057 2051 2483 2836 3498 226
Size: 378 408 45 994 432 353 662 954
Fragments arranged by size:
994 954 662 432 408 378 353 45
EcoNI CCTnn'n_nnAGG
Cuts at: 379 403 379
Size: 24 4202
EcoO109I rG'GnC_Cy
Cuts at: 154 1365 154
Size: 1211 3015
EcoRI G'AATT_C
Cuts at: 1230 1230
Size: 4226
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 4226
FspI TGC'GCA
Cuts at: 2007 2007
Size: 4226
HaeII r_GCGC'y
Cuts at: 146 346 370 429 479 488 658 707 735
Size: 200 24 59 50 9 170 49 28
Cuts at: 735 868 890 1660 1908 3138 3909 146
Size: 133 22 770 248 1230 771 463
Fragments arranged by size:
1230 771 770 463 248 200 170 133
59 50 49 28 24 22 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1202 1234 2068 2098 2100 2130 1202
Size: 32 834 30 2 30 3298
Fragments arranged by size:
3298 834 32 30 30 2
HincII GTy'rAC
Cuts at: 1151 1265 1151
Size: 114 4112
KasI G'GCGC_C
Cuts at: 142 484 703 864 886 1656 1904 142
Size: 342 219 161 22 770 248 2464
Fragments arranged by size:
2464 770 342 248 219 161 22
KpnI G_GTAC'C
Cuts at: 1246 1246
Size: 4226
MluI A'CGCG_T
Cuts at: 3659 3659
Size: 4226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2985 3169 2985
Size: 184 4042
MscI TGG'CCA
Cuts at: 1143 1987 1143
Size: 844 3382
MslI CAynn'nnrTG
Cuts at: 724 2215 2342 724
Size: 1491 127 2608
Fragments arranged by size:
2608 1491 127
MspA1I CmG'CkG
Cuts at: 118 264 318 459 490 624 633 681 911
Size: 146 54 141 31 134 9 48 230
Cuts at: 911 1040 1337 1651 2011 2795 3040 118
Size: 129 297 314 360 784 245 1304
Fragments arranged by size:
1304 784 360 314 297 245 230 146
141 134 129 54 48 31 9
NaeI GCC'GGC
Cuts at: 147 246 521 581 791 2407 147
Size: 99 275 60 210 1616 1966
Fragments arranged by size:
1966 1616 275 210 99 60
NarI GG'CG_CC
Cuts at: 143 485 704 865 887 1657 1905 143
Size: 342 219 161 22 770 248 2464
Fragments arranged by size:
2464 770 342 248 219 161 22
NcoI C'CATG_G
Cuts at: 107 107
Size: 4226
NgoMIV G'CCGG_C
Cuts at: 145 244 519 579 789 2405 145
Size: 99 275 60 210 1616 1966
Fragments arranged by size:
1966 1616 275 210 99 60
NspI r_CATG'y
Cuts at: 729 1279 1779 3382 729
Size: 550 500 1603 1573
Fragments arranged by size:
1603 1573 550 500
PasI CC'CwG_GG
Cuts at: 480 882 480
Size: 402 3824
PciI A'CATG_T
Cuts at: 3378 3378
Size: 4226
PflMI CCAn_nnn'nTGG
Cuts at: 429 684 990 1080 429
Size: 255 306 90 3575
Fragments arranged by size:
3575 306 255 90
PmeI GTTT'AAAC
Cuts at: 1225 1225
Size: 4226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 841 873 2672 2704 841
Size: 32 1799 32 2363
Fragments arranged by size:
2363 1799 32 32
PpuMI rG'GwC_Cy
Cuts at: 154 154
Size: 4226
PspOMI G'GGCC_C
Cuts at: 355 805 355
Size: 450 3776
PspXI vC'TCGA_Gb
Cuts at: 505 544 505
Size: 39 4187
PstI C_TGCA'G
Cuts at: 126 464 653 1130 1273 1958 126
Size: 338 189 477 143 685 2394
Fragments arranged by size:
2394 685 477 338 189 143
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4226
PvuII CAG'CTG
Cuts at: 264 318 624 633 681 1651 2011 264
Size: 54 306 9 48 970 360 2479
Fragments arranged by size:
2479 970 360 306 54 48 9
RsrII CG'GwC_CG
Cuts at: 2421 2421
Size: 4226
SacI G_AGCT'C
Cuts at: 1061 1240 1061
Size: 179 4047
SacII CC_GC'GG
Cuts at: 491 491
Size: 4226
SalI G'TCGA_C
Cuts at: 1263 1263
Size: 4226
SanDI GG'GwC_CC
Cuts at: 154 154
Size: 4226
SapI GCTCTTCn'nnn_
Cuts at: 352 2249 2459 352
Size: 1897 210 2119
Fragments arranged by size:
2119 1897 210
SbfI CC_TGCA'GG
Cuts at: 1273 1273
Size: 4226
ScaI AGT'ACT
Cuts at: 1000 3743 1000
Size: 2743 1483
SfcI C'TryA_G
Cuts at: 33 122 460 649 1126 1269 1954 2922 3113
Size: 89 338 189 477 143 685 968 191
Cuts at: 3113 33
Size: 1146
Fragments arranged by size:
1146 968 685 477 338 191 189 143
89
SfoI GGC'GCC
Cuts at: 144 486 705 866 888 1658 1906 144
Size: 342 219 161 22 770 248 2464
Fragments arranged by size:
2464 770 342 248 219 161 22
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
SgrAI Cr'CCGG_yG
Cuts at: 700 700
Size: 4226
SmaI CCC'GGG
Cuts at: 152 360 804 810 1248 152
Size: 208 444 6 438 3130
Fragments arranged by size:
3130 444 438 208 6
SmlI C'TyrA_G
Cuts at: 505 544 1381 2296 3010 3272 505
Size: 39 837 915 714 262 1459
Fragments arranged by size:
1459 915 837 714 262 39
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4226
SphI G_CATG'C
Cuts at: 729 1279 1779 729
Size: 550 500 3176
Fragments arranged by size:
3176 550 500
SrfI GCCC'GGGC
Cuts at: 804 804
Size: 4226
StuI AGG'CCT
Cuts at: 128 128
Size: 4226
StyI C'CwwG_G
Cuts at: 107 1360 1714 107
Size: 1253 354 2619
Fragments arranged by size:
2619 1253 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1809 1942 2401 2610 1809
Size: 133 459 209 3425
Fragments arranged by size:
3425 459 209 133
TatI w'GTAC_w
Cuts at: 998 3741 998
Size: 2743 1483
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3743 3775 3743
Size: 32 4194
Tth111I GACn'n_nGTC
Cuts at: 2023 2023
Size: 4226
XbaI T'CTAG_A
Cuts at: 1257 1257
Size: 4226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1721 1721
Size: 4226
XhoI C'TCGA_G
Cuts at: 505 544 505
Size: 39 4187
XmaI C'CCGG_G
Cuts at: 150 358 802 808 1246 150
Size: 208 444 6 438 3130
Fragments arranged by size:
3130 444 438 208 6
XmnI GAAnn'nnTTC
Cuts at: 214 214
Size: 4226
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI
BsiWI BsmI BsmBI BspEI BsrGI BstEII BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PshAI
PsiI PsrI SexAI SfiI SnaBI SspI SwaI ZraI