Restriction map for
pF1KB8677
(Circular) (Six-base) MAPSORT of: pF1KB8677.seq Check: 4528 from: 1 to: 4217
pF1KB8677 4217 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 235 1255 235
Size: 1020 3197
Acc65I G'GTAC_C
Cuts at: 1233 1233
Size: 4217
AclI AA'CG_TT
Cuts at: 4057 4057
Size: 4217
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2042 2474 2827 3489 2042
Size: 432 353 662 2770
Fragments arranged by size:
2770 662 432 353
AflIII A'CryG_T
Cuts at: 831 884 1094 1569 3369 3650 831
Size: 53 210 475 1800 281 1398
Fragments arranged by size:
1800 1398 475 281 210 53
AlwNI CAG_nnn'CTG
Cuts at: 152 516 568 1522 2960 152
Size: 364 52 954 1438 1409
Fragments arranged by size:
1438 1409 954 364 52
ApaI G_GGCC'C
Cuts at: 135 180 606 135
Size: 45 426 3746
Fragments arranged by size:
3746 426 45
ApaLI G'TGCA_C
Cuts at: 3055 3055
Size: 4217
ApoI r'AATT_y
Cuts at: 876 1221 3570 3826 876
Size: 345 2349 256 1267
Fragments arranged by size:
2349 1267 345 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4217
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4217
AvaI C'yCGr_G
Cuts at: 746 1237 746
Size: 491 3726
BamHI G'GATC_C
Cuts at: 1242 1242
Size: 4217
BanI G'GyrC_C
Cuts at: 254 315 979 1233 1647 1895 1930 254
Size: 61 664 254 414 248 35 2541
Fragments arranged by size:
2541 664 414 254 248 61 35
BanII G_rGCy'C
Cuts at: 135 180 606 797 1231 2261 135
Size: 45 426 191 434 1030 2091
Fragments arranged by size:
2091 1030 434 426 191 45
BbeI G_GCGC'C
Cuts at: 1651 1899 1651
Size: 248 3969
BbsI GAAGACnn'nnnn_
Cuts at: 650 1192 650
Size: 542 3675
BciVI GTATCCnnnnn_n'
Cuts at: 916 2115 3171 4217 916
Size: 1199 1056 1046 916
Fragments arranged by size:
1199 1056 1046 916
BclI T'GATC_A
Cuts at: 669 1737 669
Size: 1068 3149
BglI GCCn_nnn'nGGC
Cuts at: 130 130
Size: 4217
BglII A'GATC_T
Cuts at: 553 1732 3769 553
Size: 1179 2037 1001
Fragments arranged by size:
2037 1179 1001
BlpI GC'TnA_GC
Cuts at: 1329 1329
Size: 4217
Bme1580I G_kGCm'C
Cuts at: 135 180 606 1094 1105 1842 1935 3059 135
Size: 45 426 488 11 737 93 1124 1293
Fragments arranged by size:
1293 1124 737 488 426 93 45 11
BmgBI CAC'GTC
Cuts at: 1068 1068
Size: 4217
BmrI ACTGGGnnnn_n'
Cuts at: 182 1218 1429 1510 1845 3989 182
Size: 1036 211 81 335 2144 410
Fragments arranged by size:
2144 1036 410 335 211 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2584 2584
Size: 4217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 425 811 978 236
Size: 189 386 167 3475
Fragments arranged by size:
3475 386 189 167
Bpu10I CC'TnA_GC
Cuts at: 757 757
Size: 4217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1393 2272 2986 3284 1393
Size: 879 714 298 2326
Fragments arranged by size:
2326 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 334 3921 334
Size: 3587 630
BsaAI yAC'GTr
Cuts at: 832 885 1570 832
Size: 53 685 3479
Fragments arranged by size:
3479 685 53
BsaBI GATnn'nnATC
Cuts at: 6 806 1731 3768 6
Size: 800 925 2037 455
Fragments arranged by size:
2037 925 800 455
BsaHI Gr'CG_yC
Cuts at: 1648 1896 2598 4094 1648
Size: 248 702 1496 1771
Fragments arranged by size:
1771 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1406 1927 2605 3016 3163 3437 1406
Size: 521 678 411 147 274 2186
Fragments arranged by size:
2186 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1642 2400 3065 3473 1642
Size: 758 665 408 2386
Fragments arranged by size:
2386 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 166 209 1759 1805 3035 104
Size: 62 43 1550 46 1230 1286
Fragments arranged by size:
1550 1286 1230 62 46 43
BsiHKAI G_wGCw'C
Cuts at: 797 1231 2009 2199 3059 797
Size: 434 778 190 860 1955
Fragments arranged by size:
1955 860 778 434 190
BsmBI CGTCTCn'nnnn_
Cuts at: 745 1074 745
Size: 329 3888
Bsp1286I G_dGCh'C
Cuts at: 135 180 606 797 1094 1105 1231 1842 1935
Size: 45 426 191 297 11 126 611 93
Cuts at: 1935 2009 2199 2261 3059 135
Size: 74 190 62 798 1293
Fragments arranged by size:
1293 798 611 426 297 191 190 126
93 74 62 45 11
BspHI T'CATG_A
Cuts at: 2649 2649
Size: 4217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1267 1481 1783 2164 1267
Size: 214 302 381 3320
Fragments arranged by size:
3320 381 302 214
BsrBI CCG'CTC
Cuts at: 50 195 951 2509 2563 4047 4212 50
Size: 145 756 1558 54 1484 165 55
Fragments arranged by size:
1558 1484 756 165 145 55 54
BsrDI GCAATG_nn'
Cuts at: 2129 2129
Size: 4217
BsrFI r'CCGG_y
Cuts at: 2215 2396 2215
Size: 181 4036
BsrGI T'GTAC_A
Cuts at: 118 118
Size: 4217
BssSI C'ACGA_G
Cuts at: 527 2488 3196 527
Size: 1961 708 1548
Fragments arranged by size:
1961 1548 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1594 1594
Size: 4217
BstBI TT'CG_AA
Cuts at: 2578 2578
Size: 4217
BstYI r'GATC_y
Cuts at: 1 553 1242 1709 1732 2067 2313 2619 2631
Size: 552 689 467 23 335 246 306 12
Cuts at: 2631 2717 3769 1
Size: 86 1052 449
Fragments arranged by size:
1052 689 552 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1197 1197
Size: 4217
BtgI C'CryG_G
Cuts at: 107 140 468 107
Size: 33 328 3856
Fragments arranged by size:
3856 328 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2167 2348 2511 2167
Size: 181 163 3873
Fragments arranged by size:
3873 181 163
BtsI GCAGTG_nn'
Cuts at: 1460 1460
Size: 4217
DraI TTT'AAA
Cuts at: 1216 1216
Size: 4217
DrdI GACnn_nn'nnGTC
Cuts at: 1923 3267 1923
Size: 1344 2873
EaeI y'GGCC_r
Cuts at: 137 184 206 465 1802 1976 2367 2394 3739
Size: 47 22 259 1337 174 391 27 1345
Cuts at: 3739 137
Size: 615
Fragments arranged by size:
1345 1337 615 391 259 174 47 27
22
EagI C'GGCC_G
Cuts at: 206 1802 206
Size: 1596 2621
EarI CTCTTCn'nnn_
Cuts at: 2240 2450 2240
Size: 210 4007
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2425 3167 3313 4027 2425
Size: 742 146 714 2615
Fragments arranged by size:
2615 742 714 146
EcoICRI GAG'CTC
Cuts at: 795 1229 795
Size: 434 3783
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 425 811 978 2042 2474 2827 3489 236
Size: 189 386 167 1064 432 353 662 964
Fragments arranged by size:
1064 964 662 432 386 353 189 167
EcoO109I rG'GnC_Cy
Cuts at: 132 176 603 761 1356 132
Size: 44 427 158 595 2993
Fragments arranged by size:
2993 595 427 158 44
EcoRI G'AATT_C
Cuts at: 876 1221 876
Size: 345 3872
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 4217
FspI TGC'GCA
Cuts at: 1998 1998
Size: 4217
HaeII r_GCGC'y
Cuts at: 900 1651 1899 3129 3900 900
Size: 751 248 1230 771 1217
Fragments arranged by size:
1230 1217 771 751 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 371 403 510 542 954 986 2059 2089 2091
Size: 32 107 32 412 32 1073 30 2
Cuts at: 2091 2121 371
Size: 30 2467
Fragments arranged by size:
2467 1073 412 107 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 236 1256 236
Size: 1020 3197
KasI G'GCGC_C
Cuts at: 1647 1895 1647
Size: 248 3969
KpnI G_GTAC'C
Cuts at: 1237 1237
Size: 4217
MluI A'CGCG_T
Cuts at: 3650 3650
Size: 4217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 272 708 2976 3160 272
Size: 436 2268 184 1329
Fragments arranged by size:
2268 1329 436 184
MscI TGG'CCA
Cuts at: 186 467 1978 186
Size: 281 1511 2425
Fragments arranged by size:
2425 1511 281
MslI CAynn'nnrTG
Cuts at: 859 1139 2206 2333 859
Size: 280 1067 127 2743
Fragments arranged by size:
2743 1067 280 127
MspA1I CmG'CkG
Cuts at: 152 1328 1642 2002 2786 3031 152
Size: 1176 314 360 784 245 1338
Fragments arranged by size:
1338 1176 784 360 314 245
NaeI GCC'GGC
Cuts at: 2398 2398
Size: 4217
NarI GG'CG_CC
Cuts at: 1648 1896 1648
Size: 248 3969
NcoI C'CATG_G
Cuts at: 107 468 107
Size: 361 3856
NgoMIV G'CCGG_C
Cuts at: 2396 2396
Size: 4217
NspI r_CATG'y
Cuts at: 1098 1270 1770 3373 1098
Size: 172 500 1603 1942
Fragments arranged by size:
1942 1603 500 172
PciI A'CATG_T
Cuts at: 1094 3369 1094
Size: 2275 1942
PfoI T'CCnGG_A
Cuts at: 944 944
Size: 4217
PmeI GTTT'AAAC
Cuts at: 1216 1216
Size: 4217
PmlI CAC'GTG
Cuts at: 885 885
Size: 4217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2663 2695 2663
Size: 32 4185
PspOMI G'GGCC_C
Cuts at: 131 176 602 131
Size: 45 426 3746
Fragments arranged by size:
3746 426 45
PstI C_TGCA'G
Cuts at: 438 1079 1264 1949 438
Size: 641 185 685 2706
Fragments arranged by size:
2706 685 641 185
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4217
PvuII CAG'CTG
Cuts at: 1642 2002 1642
Size: 360 3857
RsrII CG'GwC_CG
Cuts at: 2412 2412
Size: 4217
SacI G_AGCT'C
Cuts at: 797 1231 797
Size: 434 3783
SalI G'TCGA_C
Cuts at: 234 1254 234
Size: 1020 3197
SapI GCTCTTCn'nnn_
Cuts at: 2240 2450 2240
Size: 210 4007
SbfI CC_TGCA'GG
Cuts at: 1264 1264
Size: 4217
ScaI AGT'ACT
Cuts at: 3734 3734
Size: 4217
SfcI C'TryA_G
Cuts at: 33 434 1075 1260 1945 2913 3104 33
Size: 401 641 185 685 968 191 1146
Fragments arranged by size:
1146 968 685 641 401 191 185
SfoI GGC'GCC
Cuts at: 1649 1897 1649
Size: 248 3969
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4217
SmaI CCC'GGG
Cuts at: 1239 1239
Size: 4217
SmlI C'TyrA_G
Cuts at: 1372 2287 3001 3263 1372
Size: 915 714 262 2326
Fragments arranged by size:
2326 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4217
SphI G_CATG'C
Cuts at: 1270 1770 1270
Size: 500 3717
StuI AGG'CCT
Cuts at: 1202 1202
Size: 4217
StyI C'CwwG_G
Cuts at: 107 187 468 476 983 1034 1351 1705 107
Size: 80 281 8 507 51 317 354 2619
Fragments arranged by size:
2619 507 354 317 281 80 51 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1800 1933 2392 2601 1800
Size: 133 459 209 3416
Fragments arranged by size:
3416 459 209 133
TatI w'GTAC_w
Cuts at: 118 887 3732 118
Size: 769 2845 603
Fragments arranged by size:
2845 769 603
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 247 279 3734 3766 247
Size: 32 3455 32 698
Fragments arranged by size:
3455 698 32 32
Tth111I GACn'n_nGTC
Cuts at: 631 2014 631
Size: 1383 2834
XbaI T'CTAG_A
Cuts at: 1248 1248
Size: 4217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 621 1712 621
Size: 1091 3126
XmaI C'CCGG_G
Cuts at: 1237 1237
Size: 4217
XmnI GAAnn'nnTTC
Cuts at: 704 704
Size: 4217
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PmlI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI
BsaXI BseRI BsgI BsiWI BsmI BspEI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI