Restriction map for
pF1KB8674
(Circular) (Six-base) MAPSORT of: pF1KB8674.seq Check: 409 from: 1 to: 4688
pF1KB8674 4688 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1269 1269
Size: 4688
AccI GT'mk_AC
Cuts at: 1726 1726
Size: 4688
Acc65I G'GTAC_C
Cuts at: 1704 1704
Size: 4688
AclI AA'CG_TT
Cuts at: 4528 4528
Size: 4688
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 1003 2513 2945 3298 3960 674
Size: 329 1510 432 353 662 1402
Fragments arranged by size:
1510 1402 662 432 353 329
AflIII A'CryG_T
Cuts at: 285 2040 3840 4121 285
Size: 1755 1800 281 852
Fragments arranged by size:
1800 1755 852 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1573 1605 1573
Size: 32 4656
AlwNI CAG_nnn'CTG
Cuts at: 568 967 1522 1993 3431 568
Size: 399 555 471 1438 1825
Fragments arranged by size:
1825 1438 555 471 399
ApaLI G'TGCA_C
Cuts at: 3526 3526
Size: 4688
ApoI r'AATT_y
Cuts at: 463 695 1413 1692 4041 4297 463
Size: 232 718 279 2349 256 854
Fragments arranged by size:
2349 854 718 279 256 232
AseI AT'TA_AT
Cuts at: 20 20
Size: 4688
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4688
AvaI C'yCGr_G
Cuts at: 1708 1708
Size: 4688
AvrII C'CTAG_G
Cuts at: 1365 1365
Size: 4688
BamHI G'GATC_C
Cuts at: 1713 1713
Size: 4688
BanI G'GyrC_C
Cuts at: 1704 2118 2366 2401 1704
Size: 414 248 35 3991
Fragments arranged by size:
3991 414 248 35
BanII G_rGCy'C
Cuts at: 1008 1702 2732 1008
Size: 694 1030 2964
Fragments arranged by size:
2964 1030 694
BbeI G_GCGC'C
Cuts at: 2122 2370 2122
Size: 248 4440
BbsI GAAGACnn'nnnn_
Cuts at: 617 617
Size: 4688
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4654
BciVI GTATCCnnnnn_n'
Cuts at: 2586 3642 4688 2586
Size: 1056 1046 2586
Fragments arranged by size:
2586 1056 1046
BclI T'GATC_A
Cuts at: 2208 2208
Size: 4688
BglII A'GATC_T
Cuts at: 1393 2203 4240 1393
Size: 810 2037 1841
Fragments arranged by size:
2037 1841 810
BlpI GC'TnA_GC
Cuts at: 1800 1800
Size: 4688
Bme1580I G_kGCm'C
Cuts at: 2313 2406 3530 2313
Size: 93 1124 3471
Fragments arranged by size:
3471 1124 93
BmgBI CAC'GTC
Cuts at: 144 144
Size: 4688
BmrI ACTGGGnnnn_n'
Cuts at: 1900 1981 2316 4460 1900
Size: 81 335 2144 2128
Fragments arranged by size:
2144 2128 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3055 3055
Size: 4688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 524 1229 524
Size: 705 3983
Bpu10I CC'TnA_GC
Cuts at: 968 968
Size: 4688
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1864 2743 3457 3755 1864
Size: 879 714 298 2797
Fragments arranged by size:
2797 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4392 4392
Size: 4688
BsaAI yAC'GTr
Cuts at: 388 2041 388
Size: 1653 3035
BsaBI GATnn'nnATC
Cuts at: 6 2202 4239 6
Size: 2196 2037 455
Fragments arranged by size:
2196 2037 455
BsaHI Gr'CG_yC
Cuts at: 2119 2367 3069 4565 2119
Size: 248 702 1496 2242
Fragments arranged by size:
2242 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1617 1877 2398 3076 3487 3634 3908 1617
Size: 260 521 678 411 147 274 2397
Fragments arranged by size:
2397 678 521 411 274 260 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 522 552 522
Size: 30 4658
BseYI C'CCAG_C
Cuts at: 2113 2871 3536 3944 2113
Size: 758 665 408 2857
Fragments arranged by size:
2857 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2230 2276 3506 104
Size: 2126 46 1230 1286
Fragments arranged by size:
2126 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1702 2480 2670 3530 1702
Size: 778 190 860 2860
Fragments arranged by size:
2860 860 778 190
BsmI GAATG_Cn'
Cuts at: 1137 1331 1137
Size: 194 4494
Bsp1286I G_dGCh'C
Cuts at: 1008 1702 2313 2406 2480 2670 2732 3530 1008
Size: 694 611 93 74 190 62 798 2166
Fragments arranged by size:
2166 798 694 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 3120 3120
Size: 4688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 535 1002 1269 1738 1952 2254 2635 535
Size: 467 267 469 214 302 381 2588
Fragments arranged by size:
2588 469 467 381 302 267 214
BsrBI CCG'CTC
Cuts at: 50 2980 3034 4518 4683 50
Size: 2930 54 1484 165 55
Fragments arranged by size:
2930 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2600 2600
Size: 4688
BsrFI r'CCGG_y
Cuts at: 2686 2867 2686
Size: 181 4507
BssSI C'ACGA_G
Cuts at: 2959 3667 2959
Size: 708 3980
BstAPI GCAn_nnn'nTGC
Cuts at: 1435 2065 1435
Size: 630 4058
BstBI TT'CG_AA
Cuts at: 3049 3049
Size: 4688
BstYI r'GATC_y
Cuts at: 1 1029 1393 1713 2180 2203 2538 2784 3090
Size: 1028 364 320 467 23 335 246 306
Cuts at: 3090 3102 3188 4240 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 1028 467 449 364 335 320 306
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2638 2819 2982 2638
Size: 181 163 4344
Fragments arranged by size:
4344 181 163
BtsI GCAGTG_nn'
Cuts at: 1931 1931
Size: 4688
DraI TTT'AAA
Cuts at: 777 887 1687 777
Size: 110 800 3778
Fragments arranged by size:
3778 800 110
DraIII CAC_nnn'GTG
Cuts at: 1309 1309
Size: 4688
DrdI GACnn_nn'nnGTC
Cuts at: 2394 3738 2394
Size: 1344 3344
EaeI y'GGCC_r
Cuts at: 2273 2447 2838 2865 4210 2273
Size: 174 391 27 1345 2751
Fragments arranged by size:
2751 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2273 2273
Size: 4688
EarI CTCTTCn'nnn_
Cuts at: 314 2711 2921 314
Size: 2397 210 2081
Fragments arranged by size:
2397 2081 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2896 3638 3784 4498 2896
Size: 742 146 714 3086
Fragments arranged by size:
3086 742 714 146
EcoICRI GAG'CTC
Cuts at: 1700 1700
Size: 4688
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 524 674 1003 1229 2513 2945 3298 3960 524
Size: 150 329 226 1284 432 353 662 1252
Fragments arranged by size:
1284 1252 662 432 353 329 226 150
EcoNI CCTnn'n_nnAGG
Cuts at: 542 542
Size: 4688
EcoO109I rG'GnC_Cy
Cuts at: 474 1369 1827 474
Size: 895 458 3335
Fragments arranged by size:
3335 895 458
EcoRI G'AATT_C
Cuts at: 1413 1692 1413
Size: 279 4409
EcoRV GAT'ATC
Cuts at: 494 1080 494
Size: 586 4102
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2707 2707
Size: 4688
FspI TGC'GCA
Cuts at: 2469 2469
Size: 4688
HaeII r_GCGC'y
Cuts at: 2122 2370 3600 4371 2122
Size: 248 1230 771 2439
Fragments arranged by size:
2439 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1014 1046 1555 1587 2530 2560 2562 2592 1014
Size: 32 509 32 943 30 2 30 3110
Fragments arranged by size:
3110 943 509 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1727 1727
Size: 4688
KasI G'GCGC_C
Cuts at: 2118 2366 2118
Size: 248 4440
KpnI G_GTAC'C
Cuts at: 1708 1708
Size: 4688
MfeI C'AATT_G
Cuts at: 819 819
Size: 4688
MluI A'CGCG_T
Cuts at: 4121 4121
Size: 4688
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 195 1057 3447 3631 195
Size: 862 2390 184 1252
Fragments arranged by size:
2390 1252 862 184
MscI TGG'CCA
Cuts at: 2449 2449
Size: 4688
MslI CAynn'nnrTG
Cuts at: 2677 2804 2677
Size: 127 4561
MspA1I CmG'CkG
Cuts at: 565 1799 2113 2473 3257 3502 565
Size: 1234 314 360 784 245 1751
Fragments arranged by size:
1751 1234 784 360 314 245
NaeI GCC'GGC
Cuts at: 2869 2869
Size: 4688
NarI GG'CG_CC
Cuts at: 2119 2367 2119
Size: 248 4440
NdeI CA'TA_TG
Cuts at: 202 202
Size: 4688
NgoMIV G'CCGG_C
Cuts at: 2867 2867
Size: 4688
NspI r_CATG'y
Cuts at: 289 1741 2241 3844 289
Size: 1452 500 1603 1133
Fragments arranged by size:
1603 1452 1133 500
PciI A'CATG_T
Cuts at: 285 3840 285
Size: 3555 1133
PflMI CCAn_nnn'nTGG
Cuts at: 824 1092 824
Size: 268 4420
PfoI T'CCnGG_A
Cuts at: 1169 1169
Size: 4688
PmeI GTTT'AAAC
Cuts at: 1687 1687
Size: 4688
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1486 1518 3134 3166 1486
Size: 32 1616 32 3008
Fragments arranged by size:
3008 1616 32 32
PpuMI rG'GwC_Cy
Cuts at: 474 1369 474
Size: 895 3793
PstI C_TGCA'G
Cuts at: 1013 1735 2420 1013
Size: 722 685 3281
Fragments arranged by size:
3281 722 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4688
PvuII CAG'CTG
Cuts at: 565 2113 2473 565
Size: 1548 360 2780
Fragments arranged by size:
2780 1548 360
RsrII CG'GwC_CG
Cuts at: 2883 2883
Size: 4688
SacI G_AGCT'C
Cuts at: 1702 1702
Size: 4688
SalI G'TCGA_C
Cuts at: 1725 1725
Size: 4688
SapI GCTCTTCn'nnn_
Cuts at: 2711 2921 2711
Size: 210 4478
SbfI CC_TGCA'GG
Cuts at: 1013 1735 1013
Size: 722 3966
ScaI AGT'ACT
Cuts at: 222 4205 222
Size: 3983 705
SfcI C'TryA_G
Cuts at: 33 173 1009 1359 1510 1731 2416 3384 3575
Size: 140 836 350 151 221 685 968 191
Cuts at: 3575 33
Size: 1146
Fragments arranged by size:
1146 968 836 685 350 221 191 151
140
SfoI GGC'GCC
Cuts at: 2120 2368 2120
Size: 248 4440
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4688
SmaI CCC'GGG
Cuts at: 1710 1710
Size: 4688
SmlI C'TyrA_G
Cuts at: 1843 2758 3472 3734 1843
Size: 915 714 262 2797
Fragments arranged by size:
2797 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4688
SphI G_CATG'C
Cuts at: 1741 2241 1741
Size: 500 4188
StyI C'CwwG_G
Cuts at: 1365 1822 2176 1365
Size: 457 354 3877
Fragments arranged by size:
3877 457 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2271 2404 2863 3072 2271
Size: 133 459 209 3887
Fragments arranged by size:
3887 459 209 133
TatI w'GTAC_w
Cuts at: 164 220 1217 4203 164
Size: 56 997 2986 649
Fragments arranged by size:
2986 997 649 56
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4205 4237 4205
Size: 32 4656
Tth111I GACn'n_nGTC
Cuts at: 2485 2485
Size: 4688
XbaI T'CTAG_A
Cuts at: 1719 1719
Size: 4688
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2183 2183
Size: 4688
XmaI C'CCGG_G
Cuts at: 1708 1708
Size: 4688
XmnI GAAnn'nnTTC
Cuts at: 464 464
Size: 4688
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI ApaI
AscI BaeI BbvCI BfrBI BglI BmtI BseRI BsgI
BsiWI BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I BtgI ClaI CspCI FseI FspAI HindIII HpaI
NcoI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI