Restriction map for
pF1KB8659
(Circular) (Six-base) MAPSORT of: pF1KB8659.seq Check: 3360 from: 1 to: 5087
pF1KB8659 5087 bp
With 182 enzymes: SgfI *
July 28, 2009 13:24 ..
AatII G_ACGT'C
Cuts at: 531 531
Size: 5087
AccI GT'mk_AC
Cuts at: 444 1450 2058 2125 444
Size: 1006 608 67 3406
Fragments arranged by size:
3406 1006 608 67
Acc65I G'GTAC_C
Cuts at: 2103 2103
Size: 5087
AclI AA'CG_TT
Cuts at: 4927 4927
Size: 5087
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 1735 2912 3344 3697 4359 241
Size: 1494 1177 432 353 662 969
Fragments arranged by size:
1494 1177 969 662 432 353
AflIII A'CryG_T
Cuts at: 1548 2439 4239 4520 1548
Size: 891 1800 281 2115
Fragments arranged by size:
2115 1800 891 281
AleI CACnn'nnGTG
Cuts at: 215 215
Size: 5087
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 791 791
Size: 5087
AlwNI CAG_nnn'CTG
Cuts at: 268 561 1138 2392 3830 268
Size: 293 577 1254 1438 1525
Fragments arranged by size:
1525 1438 1254 577 293
ApaI G_GGCC'C
Cuts at: 153 153
Size: 5087
ApaLI G'TGCA_C
Cuts at: 3925 3925
Size: 5087
ApoI r'AATT_y
Cuts at: 585 938 2091 4440 4696 585
Size: 353 1153 2349 256 976
Fragments arranged by size:
2349 1153 976 353 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5087
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5087
AvaI C'yCGr_G
Cuts at: 1957 2107 1957
Size: 150 4937
BamHI G'GATC_C
Cuts at: 2112 2112
Size: 5087
BanI G'GyrC_C
Cuts at: 599 2103 2517 2765 2800 599
Size: 1504 414 248 35 2886
Fragments arranged by size:
2886 1504 414 248 35
BanII G_rGCy'C
Cuts at: 126 153 280 1223 1993 2101 3131 126
Size: 27 127 943 770 108 1030 2082
Fragments arranged by size:
2082 1030 943 770 127 108 27
BbeI G_GCGC'C
Cuts at: 603 2521 2769 603
Size: 1918 248 2921
Fragments arranged by size:
2921 1918 248
BbsI GAAGACnn'nnnn_
Cuts at: 1058 1085 1058
Size: 27 5060
BbvCI CC'TCA_GC
Cuts at: 362 362
Size: 5087
BciVI GTATCCnnnnn_n'
Cuts at: 1209 1642 2985 4041 5087 1209
Size: 433 1343 1056 1046 1209
Fragments arranged by size:
1343 1209 1056 1046 433
BclI T'GATC_A
Cuts at: 737 1491 2607 737
Size: 754 1116 3217
Fragments arranged by size:
3217 1116 754
BglII A'GATC_T
Cuts at: 2602 4639 2602
Size: 2037 3050
BlpI GC'TnA_GC
Cuts at: 764 2199 764
Size: 1435 3652
Bme1580I G_kGCm'C
Cuts at: 153 2712 2805 3929 153
Size: 2559 93 1124 1311
Fragments arranged by size:
2559 1311 1124 93
BmgBI CAC'GTC
Cuts at: 1067 1067
Size: 5087
BmrI ACTGGGnnnn_n'
Cuts at: 1250 2299 2380 2715 4859 1250
Size: 1049 81 335 2144 1478
Fragments arranged by size:
2144 1478 1049 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3454 3454
Size: 5087
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 630 1237 630
Size: 607 4480
Bpu10I CC'TnA_GC
Cuts at: 362 362
Size: 5087
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2263 3142 3856 4154 2263
Size: 879 714 298 3196
Fragments arranged by size:
3196 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 447 4791 447
Size: 4344 743
BsaAI yAC'GTr
Cuts at: 2440 2440
Size: 5087
BsaBI GATnn'nnATC
Cuts at: 6 1601 2601 4638 6
Size: 1595 1000 2037 455
Fragments arranged by size:
2037 1595 1000 455
BsaHI Gr'CG_yC
Cuts at: 528 600 2518 2766 3468 4964 528
Size: 72 1918 248 702 1496 651
Fragments arranged by size:
1918 1496 702 651 248 72
BsaWI w'CCGG_w
Cuts at: 2276 2797 3475 3886 4033 4307 2276
Size: 521 678 411 147 274 3056
Fragments arranged by size:
3056 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 184 214 184
Size: 30 5057
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 132 406 465 1057 1060 1745 132
Size: 274 59 592 3 685 3474
Fragments arranged by size:
3474 685 592 274 59 3
BseYI C'CCAG_C
Cuts at: 141 966 2512 3270 3935 4343 141
Size: 825 1546 758 665 408 885
Fragments arranged by size:
1546 885 825 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 131 1274 2629 2675 3905 104
Size: 27 1143 1355 46 1230 1286
Fragments arranged by size:
1355 1286 1230 1143 46 27
BsiHKAI G_wGCw'C
Cuts at: 280 1072 1223 1993 2101 2879 3069 3929 280
Size: 792 151 770 108 778 190 860 1438
Fragments arranged by size:
1438 860 792 778 770 190 151 108
BsmBI CGTCTCn'nnnn_
Cuts at: 519 519
Size: 5087
Bsp1286I G_dGCh'C
Cuts at: 126 153 280 1072 1223 1993 2101 2712 2805
Size: 27 127 792 151 770 108 611 93
Cuts at: 2805 2879 3069 3131 3929 126
Size: 74 190 62 798 1284
Fragments arranged by size:
1284 798 792 770 611 190 151 127
108 93 74 62 27
BspHI T'CATG_A
Cuts at: 1664 3519 1664
Size: 1855 3232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2137 2351 2653 3034 2137
Size: 214 302 381 4190
Fragments arranged by size:
4190 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3379 3433 4917 5082 50
Size: 3329 54 1484 165 55
Fragments arranged by size:
3329 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2999 2999
Size: 5087
BsrFI r'CCGG_y
Cuts at: 130 3085 3266 130
Size: 2955 181 1951
Fragments arranged by size:
2955 1951 181
BssHII G'CGCG_C
Cuts at: 1702 1702
Size: 5087
BssSI C'ACGA_G
Cuts at: 3358 4066 3358
Size: 708 4379
BstAPI GCAn_nnn'nTGC
Cuts at: 310 1984 2464 310
Size: 1674 480 2933
Fragments arranged by size:
2933 1674 480
BstBI TT'CG_AA
Cuts at: 794 3448 794
Size: 2654 2433
BstYI r'GATC_y
Cuts at: 1 1602 1767 2112 2579 2602 2937 3183 3489
Size: 1601 165 345 467 23 335 246 306
Cuts at: 3489 3501 3587 4639 1
Size: 12 86 1052 449
Fragments arranged by size:
1601 1052 467 449 345 335 306 246
165 86 23 12
BstZ17I GTA'TAC
Cuts at: 445 445
Size: 5087
BtgI C'CryG_G
Cuts at: 107 107
Size: 5087
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3037 3218 3381 3037
Size: 181 163 4743
Fragments arranged by size:
4743 181 163
BtsI GCAGTG_nn'
Cuts at: 1982 2330 1982
Size: 348 4739
DraI TTT'AAA
Cuts at: 2086 2086
Size: 5087
DraIII CAC_nnn'GTG
Cuts at: 991 991
Size: 5087
DrdI GACnn_nn'nnGTC
Cuts at: 2793 4137 2793
Size: 1344 3743
EaeI y'GGCC_r
Cuts at: 128 833 2672 2846 3237 3264 4609 128
Size: 705 1839 174 391 27 1345 606
Fragments arranged by size:
1839 1345 705 606 391 174 27
EagI C'GGCC_G
Cuts at: 128 2672 128
Size: 2544 2543
EarI CTCTTCn'nnn_
Cuts at: 542 863 1088 1162 1758 3110 3320 542
Size: 321 225 74 596 1352 210 2309
Fragments arranged by size:
2309 1352 596 321 225 210 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3295 4037 4183 4897 3295
Size: 742 146 714 3485
Fragments arranged by size:
3485 742 714 146
EcoICRI GAG'CTC
Cuts at: 278 1221 1991 2099 278
Size: 943 770 108 3266
Fragments arranged by size:
3266 943 770 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 630 1237 1735 2912 3344 3697 4359 241
Size: 389 607 498 1177 432 353 662 969
Fragments arranged by size:
1177 969 662 607 498 432 389 353
EcoO109I rG'GnC_Cy
Cuts at: 150 2226 150
Size: 2076 3011
EcoRI G'AATT_C
Cuts at: 2091 2091
Size: 5087
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 768 3106 768
Size: 2338 2749
FspI TGC'GCA
Cuts at: 2868 2868
Size: 5087
HaeII r_GCGC'y
Cuts at: 603 2521 2769 3999 4770 603
Size: 1918 248 1230 771 920
Fragments arranged by size:
1918 1230 920 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 555 587 1488 1520 2929 2959 2961 2991 555
Size: 32 901 32 1409 30 2 30 2651
Fragments arranged by size:
2651 1409 901 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1637 1884 2126 1637
Size: 247 242 4598
Fragments arranged by size:
4598 247 242
KasI G'GCGC_C
Cuts at: 599 2517 2765 599
Size: 1918 248 2921
Fragments arranged by size:
2921 1918 248
KpnI G_GTAC'C
Cuts at: 2107 2107
Size: 5087
MfeI C'AATT_G
Cuts at: 1487 1487
Size: 5087
MluI A'CGCG_T
Cuts at: 4520 4520
Size: 5087
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1254 1642 3846 4030 1254
Size: 388 2204 184 2311
Fragments arranged by size:
2311 2204 388 184
MscI TGG'CCA
Cuts at: 2848 2848
Size: 5087
MslI CAynn'nnrTG
Cuts at: 215 1324 3076 3203 215
Size: 1109 1752 127 2099
Fragments arranged by size:
2099 1752 1109 127
MspA1I CmG'CkG
Cuts at: 174 771 1475 2198 2512 2872 3656 3901 174
Size: 597 704 723 314 360 784 245 1360
Fragments arranged by size:
1360 784 723 704 597 360 314 245
NaeI GCC'GGC
Cuts at: 3268 3268
Size: 5087
NarI GG'CG_CC
Cuts at: 600 2518 2766 600
Size: 1918 248 2921
Fragments arranged by size:
2921 1918 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5087
NgoMIV G'CCGG_C
Cuts at: 3266 3266
Size: 5087
NspI r_CATG'y
Cuts at: 1552 1656 2140 2640 4243 1552
Size: 104 484 500 1603 2396
Fragments arranged by size:
2396 1603 500 484 104
PciI A'CATG_T
Cuts at: 1548 4239 1548
Size: 2691 2396
PfoI T'CCnGG_A
Cuts at: 1758 1758
Size: 5087
PmeI GTTT'AAAC
Cuts at: 2086 2086
Size: 5087
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1493 1525 3533 3565 1493
Size: 32 2008 32 3015
Fragments arranged by size:
3015 2008 32 32
PshAI GACnn'nnGTC
Cuts at: 381 381
Size: 5087
PspOMI G'GGCC_C
Cuts at: 149 149
Size: 5087
PstI C_TGCA'G
Cuts at: 306 1105 1134 2134 2819 306
Size: 799 29 1000 685 2574
Fragments arranged by size:
2574 1000 799 685 29
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5087
PvuII CAG'CTG
Cuts at: 2512 2872 2512
Size: 360 4727
RsrII CG'GwC_CG
Cuts at: 3282 3282
Size: 5087
SacI G_AGCT'C
Cuts at: 280 1223 1993 2101 280
Size: 943 770 108 3266
Fragments arranged by size:
3266 943 770 108
SalI G'TCGA_C
Cuts at: 2124 2124
Size: 5087
SapI GCTCTTCn'nnn_
Cuts at: 3110 3320 3110
Size: 210 4877
SbfI CC_TGCA'GG
Cuts at: 2134 2134
Size: 5087
ScaI AGT'ACT
Cuts at: 4604 4604
Size: 5087
SexAI A'CCwGG_T
Cuts at: 1359 1359
Size: 5087
SfcI C'TryA_G
Cuts at: 33 302 1101 1130 2130 2815 3783 3974 33
Size: 269 799 29 1000 685 968 191 1146
Fragments arranged by size:
1146 1000 968 799 685 269 191 29
SfoI GGC'GCC
Cuts at: 601 2519 2767 601
Size: 1918 248 2921
Fragments arranged by size:
2921 1918 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5087
SmaI CCC'GGG
Cuts at: 1959 2109 1959
Size: 150 4937
SmlI C'TyrA_G
Cuts at: 2242 3157 3871 4133 2242
Size: 915 714 262 3196
Fragments arranged by size:
3196 915 714 262
SpeI A'CTAG_T
Cuts at: 67 1524 67
Size: 1457 3630
SphI G_CATG'C
Cuts at: 2140 2640 2140
Size: 500 4587
SspI AAT'ATT
Cuts at: 345 1539 345
Size: 1194 3893
StyI C'CwwG_G
Cuts at: 107 1088 1454 2221 2575 107
Size: 981 366 767 354 2619
Fragments arranged by size:
2619 981 767 366 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2670 2803 3262 3471 2670
Size: 133 459 209 4286
Fragments arranged by size:
4286 459 209 133
TatI w'GTAC_w
Cuts at: 788 1742 4602 788
Size: 954 2860 1273
Fragments arranged by size:
2860 1273 954
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 184 216 937 969 4604 4636 184
Size: 32 721 32 3635 32 635
Fragments arranged by size:
3635 721 635 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1723 2884 1723
Size: 1161 3926
XbaI T'CTAG_A
Cuts at: 516 2118 516
Size: 1602 3485
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1756 2582 1756
Size: 826 4261
XmaI C'CCGG_G
Cuts at: 1957 2107 1957
Size: 150 4937
ZraI GAC'GTC
Cuts at: 529 529
Size: 5087
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI BstZ17I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AloI AscI AvrII
BaeI BcgI BfrBI BglI BmtI BsgI BsiWI BsmI
BspEI BsrGI BstEII BstXI Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI