Restriction map for
pF1KB8655
(Circular) (Six-base) MAPSORT of: pF1KB8655.seq Check: 4575 from: 1 to: 4811
pF1KB8655 4811 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1068 1532 1068
Size: 464 4347
AccI GT'mk_AC
Cuts at: 350 434 1849 350
Size: 84 1415 3312
Fragments arranged by size:
3312 1415 84
Acc65I G'GTAC_C
Cuts at: 1827 1827
Size: 4811
AclI AA'CG_TT
Cuts at: 4651 4651
Size: 4811
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 492 1093 2636 3068 3815 4083 199
Size: 293 601 1543 432 747 268 927
Fragments arranged by size:
1543 927 747 601 432 293 268
AflIII A'CryG_T
Cuts at: 2163 3267 4244 2163
Size: 1104 977 2730
Fragments arranged by size:
2730 1104 977
AgeI A'CCGG_T
Cuts at: 3199 3199
Size: 4811
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1412 1540 1588 1412
Size: 128 48 4635
Fragments arranged by size:
4635 128 48
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 880 912 880
Size: 32 4779
AlwNI CAG_nnn'CTG
Cuts at: 265 1308 2116 3683 265
Size: 1043 808 1567 1393
Fragments arranged by size:
1567 1393 1043 808
ApaLI G'TGCA_C
Cuts at: 3581 3581
Size: 4811
ApoI r'AATT_y
Cuts at: 1122 1156 1815 4164 4420 1122
Size: 34 659 2349 256 1513
Fragments arranged by size:
2349 1513 659 256 34
AseI AT'TA_AT
Cuts at: 20 20
Size: 4811
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4811
AvaI C'yCGr_G
Cuts at: 1267 1831 1267
Size: 564 4247
BamHI G'GATC_C
Cuts at: 1836 1836
Size: 4811
BanI G'GyrC_C
Cuts at: 1827 2241 2489 2524 1827
Size: 414 248 35 4114
Fragments arranged by size:
4114 414 248 35
BanII G_rGCy'C
Cuts at: 1267 1825 2855 1267
Size: 558 1030 3223
Fragments arranged by size:
3223 1030 558
BbeI G_GCGC'C
Cuts at: 2245 2493 2245
Size: 248 4563
BbsI GAAGACnn'nnnn_
Cuts at: 386 506 1057 386
Size: 120 551 4140
Fragments arranged by size:
4140 551 120
BbvCI CC'TCA_GC
Cuts at: 1760 1760
Size: 4811
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1401 1435 1401
Size: 34 4777
BciVI GTATCCnnnnn_n'
Cuts at: 2709 3470 4811 2709
Size: 761 1341 2709
Fragments arranged by size:
2709 1341 761
BclI T'GATC_A
Cuts at: 2331 2331
Size: 4811
BglII A'GATC_T
Cuts at: 2326 4363 2326
Size: 2037 2774
BlpI GC'TnA_GC
Cuts at: 1513 1923 1513
Size: 410 4401
Bme1580I G_kGCm'C
Cuts at: 1468 2436 2529 3585 1468
Size: 968 93 1056 2694
Fragments arranged by size:
2694 1056 968 93
BmgBI CAC'GTC
Cuts at: 186 1233 186
Size: 1047 3764
BmrI ACTGGGnnnn_n'
Cuts at: 2023 2104 2439 4583 2023
Size: 81 335 2144 2251
Fragments arranged by size:
2251 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 676 3178 676
Size: 2502 2309
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1305 1454 1024
Size: 281 149 4381
Fragments arranged by size:
4381 281 149
Bpu10I CC'TnA_GC
Cuts at: 1281 1504 1597 1760 1281
Size: 223 93 163 4332
Fragments arranged by size:
4332 223 163 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 1513 1987 2866 3358 3656 316
Size: 1197 474 879 492 298 1471
Fragments arranged by size:
1471 1197 879 492 474 298
BsaI GGTCTCn'nnnn_
Cuts at: 1636 4515 1636
Size: 2879 1932
BsaAI yAC'GTr
Cuts at: 240 822 2164 240
Size: 582 1342 2887
Fragments arranged by size:
2887 1342 582
BsaBI GATnn'nnATC
Cuts at: 6 2325 4362 6
Size: 2319 2037 455
Fragments arranged by size:
2319 2037 455
BsaHI Gr'CG_yC
Cuts at: 2242 2490 3192 4688 2242
Size: 248 702 1496 2365
Fragments arranged by size:
2365 1496 702 248
BsaWI w'CCGG_w
Cuts at: 869 2000 2521 3199 3473 3620 4031 869
Size: 1131 521 678 274 147 411 1649
Fragments arranged by size:
1649 1131 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 332 362 880 910 1303 1333 332
Size: 30 518 30 393 30 3810
Fragments arranged by size:
3810 518 393 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 535 607 633 898 535
Size: 72 26 265 4448
Fragments arranged by size:
4448 265 72 26
BseYI C'CCAG_C
Cuts at: 1385 2236 2994 3571 4067 1385
Size: 851 758 577 496 2129
Fragments arranged by size:
2129 851 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 891 1201 1705 891
Size: 310 504 3997
Fragments arranged by size:
3997 504 310
BsiEI CG_ry'CG
Cuts at: 104 2353 2399 3607 104
Size: 2249 46 1208 1308
Fragments arranged by size:
2249 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 947 1825 2603 2793 3585 947
Size: 878 778 190 792 2173
Fragments arranged by size:
2173 878 792 778 190
BsmI GAATG_Cn'
Cuts at: 534 1193 534
Size: 659 4152
Bsp1286I G_dGCh'C
Cuts at: 947 1267 1468 1825 2436 2529 2603 2793 2855
Size: 320 201 357 611 93 74 190 62
Cuts at: 2855 3585 947
Size: 730 2173
Fragments arranged by size:
2173 730 611 357 320 201 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4031 4031
Size: 4811
BspHI T'CATG_A
Cuts at: 3987 3987
Size: 4811
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1068 1532 1861 2075 2377 2758 1068
Size: 464 329 214 302 381 3121
Fragments arranged by size:
3121 464 381 329 302 214
BsrBI CCG'CTC
Cuts at: 50 3103 3157 4641 4806 50
Size: 3053 54 1484 165 55
Fragments arranged by size:
3053 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1394 2723 1394
Size: 1329 3482
BsrFI r'CCGG_y
Cuts at: 1494 1556 2809 2990 3199 1494
Size: 62 1253 181 209 3106
Fragments arranged by size:
3106 1253 209 181 62
BssSI C'ACGA_G
Cuts at: 3082 3440 3082
Size: 358 4453
BstAPI GCAn_nnn'nTGC
Cuts at: 612 2188 612
Size: 1576 3235
BstBI TT'CG_AA
Cuts at: 3172 3172
Size: 4811
BstXI CCAn_nnnn'nTGG
Cuts at: 1233 1233
Size: 4811
BstYI r'GATC_y
Cuts at: 1 209 914 1836 2303 2326 2661 2907 3919
Size: 208 705 922 467 23 335 246 1012
Cuts at: 3919 4005 4017 4363 1
Size: 86 12 346 449
Fragments arranged by size:
1012 922 705 467 449 346 335 246
208 86 23 12
BstZ17I GTA'TAC
Cuts at: 435 435
Size: 4811
BtgI C'CryG_G
Cuts at: 704 899 1100 1347 1622 704
Size: 195 201 247 275 3893
Fragments arranged by size:
3893 275 247 201 195
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2761 2942 3105 2761
Size: 181 163 4467
Fragments arranged by size:
4467 181 163
BtsI GCAGTG_nn'
Cuts at: 2054 2054
Size: 4811
DraI TTT'AAA
Cuts at: 1810 1810
Size: 4811
DrdI GACnn_nn'nnGTC
Cuts at: 2517 3375 2517
Size: 858 3953
EaeI y'GGCC_r
Cuts at: 1223 2396 2570 2961 2988 4333 1223
Size: 1173 174 391 27 1345 1701
Fragments arranged by size:
1701 1345 1173 391 174 27
EagI C'GGCC_G
Cuts at: 2396 2396
Size: 4811
EarI CTCTTCn'nnn_
Cuts at: 175 512 524 584 645 1136 2834 3044 175
Size: 337 12 60 61 491 1698 210 1942
Fragments arranged by size:
1942 1698 491 337 210 61 60 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 925 3019 3329 3475 4621 925
Size: 2094 310 146 1146 1115
Fragments arranged by size:
2094 1146 1115 310 146
EcoICRI GAG'CTC
Cuts at: 1823 1823
Size: 4811
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 492 1024 1093 1305 1454 2636 3068 3815
Size: 293 532 69 212 149 1182 432 747
Cuts at: 3815 4083 199
Size: 268 927
Fragments arranged by size:
1182 927 747 532 432 293 268 212
149 69
EcoNI CCTnn'n_nnAGG
Cuts at: 1075 1075
Size: 4811
EcoO109I rG'GnC_Cy
Cuts at: 162 741 1435 1950 162
Size: 579 694 515 3023
Fragments arranged by size:
3023 694 579 515
EcoRI G'AATT_C
Cuts at: 1815 1815
Size: 4811
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2830 2830
Size: 4811
FspI TGC'GCA
Cuts at: 2592 2592
Size: 4811
HaeII r_GCGC'y
Cuts at: 849 1563 2245 2493 3515 4494 849
Size: 714 682 248 1022 979 1166
Fragments arranged by size:
1166 1022 979 714 682 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1303 1335 2653 2683 2685 2715 1303
Size: 32 1318 30 2 30 3399
Fragments arranged by size:
3399 1318 32 30 30 2
HincII GTy'rAC
Cuts at: 1850 1850
Size: 4811
KasI G'GCGC_C
Cuts at: 2241 2489 2241
Size: 248 4563
KpnI G_GTAC'C
Cuts at: 1831 1831
Size: 4811
MluI A'CGCG_T
Cuts at: 4244 4244
Size: 4811
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 753 3482 3666 753
Size: 2729 184 1898
Fragments arranged by size:
2729 1898 184
MscI TGG'CCA
Cuts at: 1225 2572 1225
Size: 1347 3464
MslI CAynn'nnrTG
Cuts at: 112 709 811 1177 2800 2927 112
Size: 597 102 366 1623 127 1996
Fragments arranged by size:
1996 1623 597 366 127 102
MspA1I CmG'CkG
Cuts at: 627 1374 1403 1696 1922 2236 2596 3609 3854
Size: 747 29 293 226 314 360 1013 245
Cuts at: 3854 627
Size: 1584
Fragments arranged by size:
1584 1013 747 360 314 293 245 226
29
NaeI GCC'GGC
Cuts at: 1496 2992 1496
Size: 1496 3315
NarI GG'CG_CC
Cuts at: 2242 2490 2242
Size: 248 4563
NcoI C'CATG_G
Cuts at: 704 1100 1347 704
Size: 396 247 4168
Fragments arranged by size:
4168 396 247
NgoMIV G'CCGG_C
Cuts at: 1494 2990 1494
Size: 1496 3315
NspI r_CATG'y
Cuts at: 756 1176 1864 2364 3271 756
Size: 420 688 500 907 2296
Fragments arranged by size:
2296 907 688 500 420
PasI CC'CwG_GG
Cuts at: 1431 1431
Size: 4811
PciI A'CATG_T
Cuts at: 3267 3267
Size: 4811
PflMI CCAn_nnn'nTGG
Cuts at: 265 1101 1308 1353 265
Size: 836 207 45 3723
Fragments arranged by size:
3723 836 207 45
PfoI T'CCnGG_A
Cuts at: 1040 1321 1711 1040
Size: 281 390 4140
Fragments arranged by size:
4140 390 281
PmeI GTTT'AAAC
Cuts at: 1810 1810
Size: 4811
PmlI CAC'GTG
Cuts at: 240 240
Size: 4811
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 880 912 3950 3982 880
Size: 32 3038 32 1709
Fragments arranged by size:
3038 1709 32 32
PpuMI rG'GwC_Cy
Cuts at: 162 741 162
Size: 579 4232
PshAI GACnn'nnGTC
Cuts at: 1061 1061
Size: 4811
PstI C_TGCA'G
Cuts at: 845 1858 2543 845
Size: 1013 685 3113
Fragments arranged by size:
3113 1013 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4811
PvuII CAG'CTG
Cuts at: 1374 1403 2236 2596 1374
Size: 29 833 360 3589
Fragments arranged by size:
3589 833 360 29
RsrII CG'GwC_CG
Cuts at: 3006 3006
Size: 4811
SacI G_AGCT'C
Cuts at: 1825 1825
Size: 4811
SalI G'TCGA_C
Cuts at: 1848 1848
Size: 4811
SapI GCTCTTCn'nnn_
Cuts at: 524 2834 3044 524
Size: 2310 210 2291
Fragments arranged by size:
2310 2291 210
SbfI CC_TGCA'GG
Cuts at: 1858 1858
Size: 4811
ScaI AGT'ACT
Cuts at: 4328 4328
Size: 4811
SfcI C'TryA_G
Cuts at: 33 841 1854 2539 3532 3723 33
Size: 808 1013 685 993 191 1121
Fragments arranged by size:
1121 1013 993 808 685 191
SfoI GGC'GCC
Cuts at: 2243 2491 2243
Size: 248 4563
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4811
SmaI CCC'GGG
Cuts at: 1833 1833
Size: 4811
SmlI C'TyrA_G
Cuts at: 295 1528 1966 2881 3373 3635 295
Size: 1233 438 915 492 262 1471
Fragments arranged by size:
1471 1233 915 492 438 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4811
SphI G_CATG'C
Cuts at: 1864 2364 1864
Size: 500 4311
StuI AGG'CCT
Cuts at: 220 220
Size: 4811
StyI C'CwwG_G
Cuts at: 704 737 1100 1347 1945 2299 704
Size: 33 363 247 598 354 3216
Fragments arranged by size:
3216 598 363 354 247 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 257 421 780 1399 1660 2394 2527 2986 3195
Size: 164 359 619 261 734 133 459 209
Cuts at: 3195 257
Size: 1873
Fragments arranged by size:
1873 734 619 459 359 261 209 164
133
TatI w'GTAC_w
Cuts at: 1445 4326 1445
Size: 2881 1930
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 181 213 4328 4360 181
Size: 32 4115 32 632
Fragments arranged by size:
4115 632 32 32
Tth111I GACn'n_nGTC
Cuts at: 696 1234 2608 696
Size: 538 1374 2899
Fragments arranged by size:
2899 1374 538
XbaI T'CTAG_A
Cuts at: 1842 1842
Size: 4811
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 266 1254 1329 2306 266
Size: 988 75 977 2771
Fragments arranged by size:
2771 988 977 75
XmaI C'CCGG_G
Cuts at: 1831 1831
Size: 4811
XmnI GAAnn'nnTTC
Cuts at: 311 311
Size: 4811
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI ApaI AscI AvrII
BaeI BfrBI BglI BmtI BsiWI BsmBI BsrGI BssHII
BstEII Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI