Restriction map for
pF1KB8645
(Circular) (Six-base) MAPSORT of: pF1KB8645.seq Check: 5215 from: 1 to: 3911
pF1KB8645 3911 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 284 949 284
Size: 665 3246
Acc65I G'GTAC_C
Cuts at: 927 927
Size: 3911
AclI AA'CG_TT
Cuts at: 3751 3751
Size: 3911
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 496 651 1736 2168 2521 3183 427
Size: 69 155 1085 432 353 662 1155
Fragments arranged by size:
1155 1085 662 432 353 155 69
AflIII A'CryG_T
Cuts at: 302 1263 3063 3344 302
Size: 961 1800 281 869
Fragments arranged by size:
1800 961 869 281
AleI CACnn'nnGTG
Cuts at: 640 640
Size: 3911
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 193 193
Size: 3911
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 637 669 637
Size: 32 3879
AlwNI CAG_nnn'CTG
Cuts at: 456 1216 2654 456
Size: 760 1438 1713
Fragments arranged by size:
1713 1438 760
ApaLI G'TGCA_C
Cuts at: 894 2749 894
Size: 1855 2056
ApoI r'AATT_y
Cuts at: 915 3264 3520 915
Size: 2349 256 1306
Fragments arranged by size:
2349 1306 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3911
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3911
AvaI C'yCGr_G
Cuts at: 191 931 191
Size: 740 3171
BamHI G'GATC_C
Cuts at: 936 936
Size: 3911
BanI G'GyrC_C
Cuts at: 184 558 927 1341 1589 1624 184
Size: 374 369 414 248 35 2471
Fragments arranged by size:
2471 414 374 369 248 35
BanII G_rGCy'C
Cuts at: 208 700 925 1955 208
Size: 492 225 1030 2164
Fragments arranged by size:
2164 1030 492 225
BbeI G_GCGC'C
Cuts at: 1345 1593 1345
Size: 248 3663
BbsI GAAGACnn'nnnn_
Cuts at: 121 378 121
Size: 257 3654
BciVI GTATCCnnnnn_n'
Cuts at: 318 1809 2865 3911 318
Size: 1491 1056 1046 318
Fragments arranged by size:
1491 1056 1046 318
BclI T'GATC_A
Cuts at: 1431 1431
Size: 3911
BglII A'GATC_T
Cuts at: 716 1426 3463 716
Size: 710 2037 1164
Fragments arranged by size:
2037 1164 710
BlpI GC'TnA_GC
Cuts at: 1023 1023
Size: 3911
Bme1580I G_kGCm'C
Cuts at: 152 563 898 1536 1629 2753 152
Size: 411 335 638 93 1124 1310
Fragments arranged by size:
1310 1124 638 411 335 93
BmrI ACTGGGnnnn_n'
Cuts at: 1123 1204 1539 3683 1123
Size: 81 335 2144 1351
Fragments arranged by size:
2144 1351 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2278 2278
Size: 3911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 478
Size: 3911
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 823 1087 1966 2680 2978 823
Size: 264 879 714 298 1756
Fragments arranged by size:
1756 879 714 298 264
BsaI GGTCTCn'nnnn_
Cuts at: 804 3615 804
Size: 2811 1100
BsaAI yAC'GTr
Cuts at: 660 1264 660
Size: 604 3307
BsaBI GATnn'nnATC
Cuts at: 6 1425 3462 6
Size: 1419 2037 455
Fragments arranged by size:
2037 1419 455
BsaHI Gr'CG_yC
Cuts at: 737 785 1342 1590 2292 3788 737
Size: 48 557 248 702 1496 860
Fragments arranged by size:
1496 860 702 557 248 48
BsaWI w'CCGG_w
Cuts at: 1100 1621 2299 2710 2857 3131 1100
Size: 521 678 411 147 274 1880
Fragments arranged by size:
1880 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 451 481 451
Size: 30 3881
BseYI C'CCAG_C
Cuts at: 180 820 1336 2094 2759 3167 180
Size: 640 516 758 665 408 924
Fragments arranged by size:
924 758 665 640 516 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 745 120
Size: 625 3286
BsiEI CG_ry'CG
Cuts at: 104 1453 1499 2729 104
Size: 1349 46 1230 1286
Fragments arranged by size:
1349 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 208 665 898 925 1703 1893 2753 208
Size: 457 233 27 778 190 860 1366
Fragments arranged by size:
1366 860 778 457 233 190 27
Bsp1286I G_dGCh'C
Cuts at: 152 208 563 665 700 898 925 1536 1629
Size: 56 355 102 35 198 27 611 93
Cuts at: 1629 1703 1893 1955 2753 152
Size: 74 190 62 798 1310
Fragments arranged by size:
1310 798 611 355 198 190 102 93
74 62 56 35 27
BspHI T'CATG_A
Cuts at: 2343 2343
Size: 3911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 961 1175 1477 1858 961
Size: 214 302 381 3014
Fragments arranged by size:
3014 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2203 2257 3741 3906 50
Size: 2153 54 1484 165 55
Fragments arranged by size:
2153 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1823 1823
Size: 3911
BsrFI r'CCGG_y
Cuts at: 1909 2090 1909
Size: 181 3730
BssSI C'ACGA_G
Cuts at: 688 2182 2890 688
Size: 1494 708 1709
Fragments arranged by size:
1709 1494 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1288 1288
Size: 3911
BstBI TT'CG_AA
Cuts at: 2272 2272
Size: 3911
BstYI r'GATC_y
Cuts at: 1 452 716 936 1403 1426 1761 2007 2313
Size: 451 264 220 467 23 335 246 306
Cuts at: 2313 2325 2411 3463 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 467 451 449 335 306 264 246
220 86 23 12
BstZ17I GTA'TAC
Cuts at: 285 285
Size: 3911
BtgI C'CryG_G
Cuts at: 294 509 537 294
Size: 215 28 3668
Fragments arranged by size:
3668 215 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 694 1861 2042 2205 694
Size: 1167 181 163 2400
Fragments arranged by size:
2400 1167 181 163
BtsI GCAGTG_nn'
Cuts at: 1154 1154
Size: 3911
DraI TTT'AAA
Cuts at: 910 910
Size: 3911
DrdI GACnn_nn'nnGTC
Cuts at: 1617 2961 1617
Size: 1344 2567
EaeI y'GGCC_r
Cuts at: 231 623 674 1496 1670 2061 2088 3433 231
Size: 392 51 822 174 391 27 1345 709
Fragments arranged by size:
1345 822 709 392 391 174 51 27
EagI C'GGCC_G
Cuts at: 1496 1496
Size: 3911
EarI CTCTTCn'nnn_
Cuts at: 670 1934 2144 670
Size: 1264 210 2437
Fragments arranged by size:
2437 1264 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 514 2119 2861 3007 3721 514
Size: 1605 742 146 714 704
Fragments arranged by size:
1605 742 714 704 146
EcoICRI GAG'CTC
Cuts at: 206 923 206
Size: 717 3194
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 478 496 651 1736 2168 2521 3183 427
Size: 51 18 155 1085 432 353 662 1155
Fragments arranged by size:
1155 1085 662 432 353 155 51 18
EcoO109I rG'GnC_Cy
Cuts at: 752 858 1050 752
Size: 106 192 3613
Fragments arranged by size:
3613 192 106
EcoRI G'AATT_C
Cuts at: 915 915
Size: 3911
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1930 1930
Size: 3911
FspI TGC'GCA
Cuts at: 358 1692 358
Size: 1334 2577
HaeII r_GCGC'y
Cuts at: 441 1345 1593 2823 3594 441
Size: 904 248 1230 771 758
Fragments arranged by size:
1230 904 771 758 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 451 483 1753 1783 1785 1815 451
Size: 32 1270 30 2 30 2547
Fragments arranged by size:
2547 1270 32 30 30 2
HincII GTy'rAC
Cuts at: 828 950 828
Size: 122 3789
KasI G'GCGC_C
Cuts at: 1341 1589 1341
Size: 248 3663
KpnI G_GTAC'C
Cuts at: 931 931
Size: 3911
MluI A'CGCG_T
Cuts at: 3344 3344
Size: 3911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2670 2854 2670
Size: 184 3727
MscI TGG'CCA
Cuts at: 676 1672 676
Size: 996 2915
MslI CAynn'nnrTG
Cuts at: 175 394 640 1900 2027 175
Size: 219 246 1260 127 2059
Fragments arranged by size:
2059 1260 246 219 127
MspA1I CmG'CkG
Cuts at: 128 296 1022 1336 1696 2480 2725 128
Size: 168 726 314 360 784 245 1314
Fragments arranged by size:
1314 784 726 360 314 245 168
NaeI GCC'GGC
Cuts at: 2092 2092
Size: 3911
NarI GG'CG_CC
Cuts at: 1342 1590 1342
Size: 248 3663
NcoI C'CATG_G
Cuts at: 509 509
Size: 3911
NgoMIV G'CCGG_C
Cuts at: 2090 2090
Size: 3911
NspI r_CATG'y
Cuts at: 306 765 964 1464 3067 306
Size: 459 199 500 1603 1150
Fragments arranged by size:
1603 1150 500 459 199
PciI A'CATG_T
Cuts at: 302 3063 302
Size: 2761 1150
PfoI T'CCnGG_A
Cuts at: 320 455 320
Size: 135 3776
PmeI GTTT'AAAC
Cuts at: 910 910
Size: 3911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2357 2389 2357
Size: 32 3879
PpuMI rG'GwC_Cy
Cuts at: 858 858
Size: 3911
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 592 624 592
Size: 32 3879
PstI C_TGCA'G
Cuts at: 958 1643 958
Size: 685 3226
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3911
PvuII CAG'CTG
Cuts at: 1336 1696 1336
Size: 360 3551
RsrII CG'GwC_CG
Cuts at: 2106 2106
Size: 3911
SacI G_AGCT'C
Cuts at: 208 925 208
Size: 717 3194
SacII CC_GC'GG
Cuts at: 297 297
Size: 3911
SalI G'TCGA_C
Cuts at: 948 948
Size: 3911
SapI GCTCTTCn'nnn_
Cuts at: 670 1934 2144 670
Size: 1264 210 2437
Fragments arranged by size:
2437 1264 210
SbfI CC_TGCA'GG
Cuts at: 958 958
Size: 3911
ScaI AGT'ACT
Cuts at: 3428 3428
Size: 3911
SfcI C'TryA_G
Cuts at: 33 954 1639 2607 2798 33
Size: 921 685 968 191 1146
Fragments arranged by size:
1146 968 921 685 191
SfoI GGC'GCC
Cuts at: 1343 1591 1343
Size: 248 3663
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3911
SmaI CCC'GGG
Cuts at: 933 933
Size: 3911
SmlI C'TyrA_G
Cuts at: 838 1066 1981 2695 2957 838
Size: 228 915 714 262 1792
Fragments arranged by size:
1792 915 714 262 228
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3911
SphI G_CATG'C
Cuts at: 765 964 1464 765
Size: 199 500 3212
Fragments arranged by size:
3212 500 199
StyI C'CwwG_G
Cuts at: 488 509 854 1045 1399 488
Size: 21 345 191 354 3000
Fragments arranged by size:
3000 354 345 191 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 172 270 1494 1627 2086 2295 172
Size: 98 1224 133 459 209 1788
Fragments arranged by size:
1788 1224 459 209 133 98
TatI w'GTAC_w
Cuts at: 248 842 3426 248
Size: 594 2584 733
Fragments arranged by size:
2584 733 594
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3428 3460 3428
Size: 32 3879
Tth111I GACn'n_nGTC
Cuts at: 120 1708 120
Size: 1588 2323
XbaI T'CTAG_A
Cuts at: 942 942
Size: 3911
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1406 1406
Size: 3911
XmaI C'CCGG_G
Cuts at: 931 931
Size: 3911
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BseRI BsiWI BsmI BsmBI BspEI BsrGI BssHII
BstEII BstXI Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspOMI PspXI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI