Restriction map for
pF1KB8640
(Circular) (Six-base) MAPSORT of: pF1KB8640.seq Check: 9090 from: 1 to: 3722
pF1KB8640 3722 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AatII G_ACGT'C
Cuts at: 293 293
Size: 3722
AccI GT'mk_AC
Cuts at: 263 383 760 263
Size: 120 377 3225
Fragments arranged by size:
3225 377 120
Acc65I G'GTAC_C
Cuts at: 738 738
Size: 3722
AclI AA'CG_TT
Cuts at: 3562 3562
Size: 3722
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1547 1979 2726 2994 1547
Size: 432 747 268 2275
Fragments arranged by size:
2275 747 432 268
AflIII A'CryG_T
Cuts at: 1074 2178 3155 1074
Size: 1104 977 1641
Fragments arranged by size:
1641 1104 977
AgeI A'CCGG_T
Cuts at: 2110 2110
Size: 3722
AlwNI CAG_nnn'CTG
Cuts at: 513 560 1027 2594 513
Size: 47 467 1567 1641
Fragments arranged by size:
1641 1567 467 47
ApaLI G'TGCA_C
Cuts at: 2492 2492
Size: 3722
ApoI r'AATT_y
Cuts at: 726 3075 3331 726
Size: 2349 256 1117
Fragments arranged by size:
2349 1117 256
AscI GG'CGCG_CC
Cuts at: 461 461
Size: 3722
AseI AT'TA_AT
Cuts at: 20 20
Size: 3722
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3722
AvaI C'yCGr_G
Cuts at: 144 196 306 313 577 742 144
Size: 52 110 7 264 165 3124
Fragments arranged by size:
3124 264 165 110 52 7
BamHI G'GATC_C
Cuts at: 747 747
Size: 3722
BanI G'GyrC_C
Cuts at: 139 256 738 1152 1400 1435 139
Size: 117 482 414 248 35 2426
Fragments arranged by size:
2426 482 414 248 117 35
BanII G_rGCy'C
Cuts at: 313 736 1766 313
Size: 423 1030 2269
Fragments arranged by size:
2269 1030 423
BbeI G_GCGC'C
Cuts at: 143 260 1156 1404 143
Size: 117 896 248 2461
Fragments arranged by size:
2461 896 248 117
BciVI GTATCCnnnnn_n'
Cuts at: 1620 2381 3722 1620
Size: 761 1341 1620
Fragments arranged by size:
1620 1341 761
BclI T'GATC_A
Cuts at: 1242 1242
Size: 3722
BglII A'GATC_T
Cuts at: 1237 3274 1237
Size: 2037 1685
BlpI GC'TnA_GC
Cuts at: 834 834
Size: 3722
Bme1580I G_kGCm'C
Cuts at: 1347 1440 2496 1347
Size: 93 1056 2573
Fragments arranged by size:
2573 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 934 1015 1350 3494 934
Size: 81 335 2144 1162
Fragments arranged by size:
2144 1162 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2089 2089
Size: 3722
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 369 369
Size: 3722
Bpu10I CC'TnA_GC
Cuts at: 341 341
Size: 3722
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 898 1777 2269 2567 291
Size: 607 879 492 298 1446
Fragments arranged by size:
1446 879 607 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 439 3426 439
Size: 2987 735
BsaAI yAC'GTr
Cuts at: 417 606 1075 417
Size: 189 469 3064
Fragments arranged by size:
3064 469 189
BsaBI GATnn'nnATC
Cuts at: 6 1236 3273 6
Size: 1230 2037 455
Fragments arranged by size:
2037 1230 455
BsaHI Gr'CG_yC
Cuts at: 140 257 290 368 1153 1401 2103 3599 140
Size: 117 33 78 785 248 702 1496 263
Fragments arranged by size:
1496 785 702 263 248 117 78 33
BsaWI w'CCGG_w
Cuts at: 321 911 1432 2110 2384 2531 2942 321
Size: 590 521 678 274 147 411 1101
Fragments arranged by size:
1101 678 590 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 340 340
Size: 3722
BseYI C'CCAG_C
Cuts at: 1147 1905 2482 2978 1147
Size: 758 577 496 1891
Fragments arranged by size:
1891 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 644 644
Size: 3722
BsiEI CG_ry'CG
Cuts at: 104 1264 1310 2518 104
Size: 1160 46 1208 1308
Fragments arranged by size:
1308 1208 1160 46
BsiHKAI G_wGCw'C
Cuts at: 380 586 736 1514 1704 2496 380
Size: 206 150 778 190 792 1606
Fragments arranged by size:
1606 792 778 206 190 150
BsmBI CGTCTCn'nnnn_
Cuts at: 619 619
Size: 3722
Bsp1286I G_dGCh'C
Cuts at: 313 380 586 736 1347 1440 1514 1704 1766
Size: 67 206 150 611 93 74 190 62
Cuts at: 1766 2496 313
Size: 730 1539
Fragments arranged by size:
1539 730 611 206 190 150 93 74
67 62
BspEI T'CCGG_A
Cuts at: 321 2942 321
Size: 2621 1101
BspHI T'CATG_A
Cuts at: 2898 2898
Size: 3722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 772 986 1288 1669 772
Size: 214 302 381 2825
Fragments arranged by size:
2825 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2014 2068 3552 3717 50
Size: 1964 54 1484 165 55
Fragments arranged by size:
1964 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1634 1634
Size: 3722
BsrFI r'CCGG_y
Cuts at: 206 453 1720 1901 2110 206
Size: 247 1267 181 209 1818
Fragments arranged by size:
1818 1267 247 209 181
BssHII G'CGCG_C
Cuts at: 461 531 533 648 650 652 700 461
Size: 70 2 115 2 2 48 3483
Fragments arranged by size:
3483 115 70 48 2 2 2
BssSI C'ACGA_G
Cuts at: 481 1993 2351 481
Size: 1512 358 1852
Fragments arranged by size:
1852 1512 358
BstAPI GCAn_nnn'nTGC
Cuts at: 513 609 1099 513
Size: 96 490 3136
Fragments arranged by size:
3136 490 96
BstBI TT'CG_AA
Cuts at: 2083 2083
Size: 3722
BstXI CCAn_nnnn'nTGG
Cuts at: 483 483
Size: 3722
BstYI r'GATC_y
Cuts at: 1 747 1214 1237 1572 1818 2830 2916 2928
Size: 746 467 23 335 246 1012 86 12
Cuts at: 2928 3274 1
Size: 346 449
Fragments arranged by size:
1012 746 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 212 239 526 107
Size: 105 27 287 3303
Fragments arranged by size:
3303 287 105 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 423 1672 1853 2016 423
Size: 1249 181 163 2129
Fragments arranged by size:
2129 1249 181 163
BtsI GCAGTG_nn'
Cuts at: 965 965
Size: 3722
DraI TTT'AAA
Cuts at: 721 721
Size: 3722
DrdI GACnn_nn'nnGTC
Cuts at: 259 289 1428 2286 259
Size: 30 1139 858 1695
Fragments arranged by size:
1695 1139 858 30
EaeI y'GGCC_r
Cuts at: 128 161 331 539 1307 1481 1872 1899 3244
Size: 33 170 208 768 174 391 27 1345
Cuts at: 3244 128
Size: 606
Fragments arranged by size:
1345 768 606 391 208 174 170 33
27
EagI C'GGCC_G
Cuts at: 1307 1307
Size: 3722
EarI CTCTTCn'nnn_
Cuts at: 622 1745 1955 622
Size: 1123 210 2389
Fragments arranged by size:
2389 1123 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 160 576 1930 2240 2386 3532 160
Size: 416 1354 310 146 1146 350
Fragments arranged by size:
1354 1146 416 350 310 146
EcoICRI GAG'CTC
Cuts at: 734 734
Size: 3722
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 369 1547 1979 2726 2994 369
Size: 1178 432 747 268 1097
Fragments arranged by size:
1178 1097 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 423 861 423
Size: 438 3284
EcoRI G'AATT_C
Cuts at: 726 726
Size: 3722
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1741 1741
Size: 3722
FspI TGC'GCA
Cuts at: 553 1503 553
Size: 950 2772
HaeII r_GCGC'y
Cuts at: 143 204 260 475 567 691 1156 1404 2426
Size: 61 56 215 92 124 465 248 1022
Cuts at: 2426 3405 143
Size: 979 460
Fragments arranged by size:
1022 979 465 460 248 215 124 92
61 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1564 1594 1596 1626 1564
Size: 30 2 30 3660
Fragments arranged by size:
3660 30 30 2
HincII GTy'rAC
Cuts at: 761 761
Size: 3722
KasI G'GCGC_C
Cuts at: 139 256 1152 1400 139
Size: 117 896 248 2461
Fragments arranged by size:
2461 896 248 117
KpnI G_GTAC'C
Cuts at: 742 742
Size: 3722
MluI A'CGCG_T
Cuts at: 3155 3155
Size: 3722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2393 2577 2393
Size: 184 3538
MscI TGG'CCA
Cuts at: 333 1483 333
Size: 1150 2572
MslI CAynn'nnrTG
Cuts at: 481 1711 1838 481
Size: 1230 127 2365
Fragments arranged by size:
2365 1230 127
MspA1I CmG'CkG
Cuts at: 241 510 528 833 1147 1507 2520 2765 241
Size: 269 18 305 314 360 1013 245 1198
Fragments arranged by size:
1198 1013 360 314 305 269 245 18
NaeI GCC'GGC
Cuts at: 455 1903 455
Size: 1448 2274
NarI GG'CG_CC
Cuts at: 140 257 1153 1401 140
Size: 117 896 248 2461
Fragments arranged by size:
2461 896 248 117
NcoI C'CATG_G
Cuts at: 107 107
Size: 3722
NgoMIV G'CCGG_C
Cuts at: 453 1901 453
Size: 1448 2274
NspI r_CATG'y
Cuts at: 775 1275 2182 775
Size: 500 907 2315
Fragments arranged by size:
2315 907 500
PciI A'CATG_T
Cuts at: 2178 2178
Size: 3722
PmeI GTTT'AAAC
Cuts at: 721 721
Size: 3722
PmlI CAC'GTG
Cuts at: 606 606
Size: 3722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2861 2893 2861
Size: 32 3690
PspXI vC'TCGA_Gb
Cuts at: 306 577 306
Size: 271 3451
PstI C_TGCA'G
Cuts at: 397 683 709 769 1454 397
Size: 286 26 60 685 2665
Fragments arranged by size:
2665 685 286 60 26
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3722
PvuII CAG'CTG
Cuts at: 1147 1507 1147
Size: 360 3362
RsrII CG'GwC_CG
Cuts at: 523 1917 523
Size: 1394 2328
SacI G_AGCT'C
Cuts at: 736 736
Size: 3722
SacII CC_GC'GG
Cuts at: 242 529 242
Size: 287 3435
SalI G'TCGA_C
Cuts at: 759 759
Size: 3722
SapI GCTCTTCn'nnn_
Cuts at: 1745 1955 1745
Size: 210 3512
SbfI CC_TGCA'GG
Cuts at: 683 769 683
Size: 86 3636
ScaI AGT'ACT
Cuts at: 3239 3239
Size: 3722
SfcI C'TryA_G
Cuts at: 33 393 679 705 765 1450 2443 2634 33
Size: 360 286 26 60 685 993 191 1121
Fragments arranged by size:
1121 993 685 360 286 191 60 26
SfoI GGC'GCC
Cuts at: 141 258 1154 1402 141
Size: 117 896 248 2461
Fragments arranged by size:
2461 896 248 117
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3722
SmaI CCC'GGG
Cuts at: 146 315 744 146
Size: 169 429 3124
Fragments arranged by size:
3124 429 169
SmlI C'TyrA_G
Cuts at: 270 306 577 877 1792 2284 2546 270
Size: 36 271 300 915 492 262 1446
Fragments arranged by size:
1446 915 492 300 271 262 36
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3722
SphI G_CATG'C
Cuts at: 775 1275 775
Size: 500 3222
StyI C'CwwG_G
Cuts at: 107 856 1210 107
Size: 749 354 2619
Fragments arranged by size:
2619 749 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1305 1438 1897 2106 1305
Size: 133 459 209 2921
Fragments arranged by size:
2921 459 209 133
TatI w'GTAC_w
Cuts at: 593 3237 593
Size: 2644 1078
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3239 3271 3239
Size: 32 3690
Tth111I GACn'n_nGTC
Cuts at: 1519 1519
Size: 3722
XbaI T'CTAG_A
Cuts at: 753 753
Size: 3722
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1217 1217
Size: 3722
XhoI C'TCGA_G
Cuts at: 306 577 306
Size: 271 3451
XmaI C'CCGG_G
Cuts at: 144 313 742 144
Size: 169 429 3124
Fragments arranged by size:
3124 429 169
ZraI GAC'GTC
Cuts at: 291 291
Size: 3722
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PmlI PpiI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmI BsrGI BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PpuMI PshAI PsiI PspOMI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XmnI