Restriction map for
pF1KB8639
(Circular) (Six-base) MAPSORT of: pF1KB8639.seq Check: 6559 from: 1 to: 4097
pF1KB8639 4097 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 625 625
Size: 4097
AccI GT'mk_AC
Cuts at: 1135 1135
Size: 4097
Acc65I G'GTAC_C
Cuts at: 1113 1113
Size: 4097
AclI AA'CG_TT
Cuts at: 3937 3937
Size: 4097
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 1922 2354 3101 3369 343
Size: 1579 432 747 268 1071
Fragments arranged by size:
1579 1071 747 432 268
AflIII A'CryG_T
Cuts at: 1449 2553 3530 1449
Size: 1104 977 2016
Fragments arranged by size:
2016 1104 977
AgeI A'CCGG_T
Cuts at: 2485 2485
Size: 4097
AlwNI CAG_nnn'CTG
Cuts at: 346 475 1402 2969 346
Size: 129 927 1567 1474
Fragments arranged by size:
1567 1474 927 129
ApaLI G'TGCA_C
Cuts at: 2867 2867
Size: 4097
ApoI r'AATT_y
Cuts at: 489 497 652 902 1026 1101 3450 3706 489
Size: 8 155 250 124 75 2349 256 880
Fragments arranged by size:
2349 880 256 250 155 124 75 8
AseI AT'TA_AT
Cuts at: 20 20
Size: 4097
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
AvaI C'yCGr_G
Cuts at: 1117 1117
Size: 4097
BamHI G'GATC_C
Cuts at: 1122 1122
Size: 4097
BanI G'GyrC_C
Cuts at: 1113 1527 1775 1810 1113
Size: 414 248 35 3400
Fragments arranged by size:
3400 414 248 35
BanII G_rGCy'C
Cuts at: 756 1111 2141 756
Size: 355 1030 2712
Fragments arranged by size:
2712 1030 355
BbeI G_GCGC'C
Cuts at: 1531 1779 1531
Size: 248 3849
BciVI GTATCCnnnnn_n'
Cuts at: 1995 2756 4097 1995
Size: 761 1341 1995
Fragments arranged by size:
1995 1341 761
BclI T'GATC_A
Cuts at: 1617 1617
Size: 4097
BglII A'GATC_T
Cuts at: 1612 3649 1612
Size: 2037 2060
BlpI GC'TnA_GC
Cuts at: 1209 1209
Size: 4097
Bme1580I G_kGCm'C
Cuts at: 1722 1815 2871 1722
Size: 93 1056 2948
Fragments arranged by size:
2948 1056 93
BmgBI CAC'GTC
Cuts at: 981 981
Size: 4097
BmrI ACTGGGnnnn_n'
Cuts at: 1309 1390 1725 3869 1309
Size: 81 335 2144 1537
Fragments arranged by size:
2144 1537 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 4097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 1059 1273 2152 2644 2942 562
Size: 497 214 879 492 298 1717
Fragments arranged by size:
1717 879 497 492 298 214
BsaI GGTCTCn'nnnn_
Cuts at: 3801 3801
Size: 4097
BsaAI yAC'GTr
Cuts at: 1450 1450
Size: 4097
BsaBI GATnn'nnATC
Cuts at: 6 1611 3648 6
Size: 1605 2037 455
Fragments arranged by size:
2037 1605 455
BsaHI Gr'CG_yC
Cuts at: 1528 1776 2478 3974 1528
Size: 248 702 1496 1651
Fragments arranged by size:
1651 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1286 1807 2485 2759 2906 3317 1286
Size: 521 678 274 147 411 2066
Fragments arranged by size:
2066 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1522 2280 2857 3353 1522
Size: 758 577 496 2266
Fragments arranged by size:
2266 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 743 743
Size: 4097
BsiEI CG_ry'CG
Cuts at: 104 1639 1685 2893 104
Size: 1535 46 1208 1308
Fragments arranged by size:
1535 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1111 1889 2079 2871 1111
Size: 778 190 792 2337
Fragments arranged by size:
2337 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 756 1111 1722 1815 1889 2079 2141 2871 756
Size: 355 611 93 74 190 62 730 1982
Fragments arranged by size:
1982 730 611 355 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3317 3317
Size: 4097
BspHI T'CATG_A
Cuts at: 3273 3273
Size: 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 625 1147 1361 1663 2044 625
Size: 522 214 302 381 2678
Fragments arranged by size:
2678 522 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2389 2443 3927 4092 50
Size: 2339 54 1484 165 55
Fragments arranged by size:
2339 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 447 945 2009 447
Size: 498 1064 2535
Fragments arranged by size:
2535 1064 498
BsrFI r'CCGG_y
Cuts at: 2095 2276 2485 2095
Size: 181 209 3707
Fragments arranged by size:
3707 209 181
BssSI C'ACGA_G
Cuts at: 2368 2726 2368
Size: 358 3739
BstAPI GCAn_nnn'nTGC
Cuts at: 1474 1474
Size: 4097
BstBI TT'CG_AA
Cuts at: 2458 2458
Size: 4097
BstYI r'GATC_y
Cuts at: 1 119 1122 1589 1612 1947 2193 3205 3291
Size: 118 1003 467 23 335 246 1012 86
Cuts at: 3291 3303 3649 1
Size: 12 346 449
Fragments arranged by size:
1012 1003 467 449 346 335 246 118
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 309 309
Size: 4097
BtgI C'CryG_G
Cuts at: 107 242 107
Size: 135 3962
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2047 2228 2391 2047
Size: 181 163 3753
Fragments arranged by size:
3753 181 163
BtsI GCAGTG_nn'
Cuts at: 1340 1340
Size: 4097
DraI TTT'AAA
Cuts at: 1096 1096
Size: 4097
DrdI GACnn_nn'nnGTC
Cuts at: 1803 2661 1803
Size: 858 3239
EaeI y'GGCC_r
Cuts at: 1682 1856 2247 2274 3619 1682
Size: 174 391 27 1345 2160
Fragments arranged by size:
2160 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1682 1682
Size: 4097
EarI CTCTTCn'nnn_
Cuts at: 572 983 2120 2330 572
Size: 411 1137 210 2339
Fragments arranged by size:
2339 1137 411 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2305 2615 2761 3907 2305
Size: 310 146 1146 2495
Fragments arranged by size:
2495 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1109 1109
Size: 4097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 1922 2354 3101 3369 343
Size: 1579 432 747 268 1071
Fragments arranged by size:
1579 1071 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 221 221
Size: 4097
EcoO109I rG'GnC_Cy
Cuts at: 183 701 1236 183
Size: 518 535 3044
Fragments arranged by size:
3044 535 518
EcoRI G'AATT_C
Cuts at: 902 1101 902
Size: 199 3898
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 4097
FspI TGC'GCA
Cuts at: 1878 1878
Size: 4097
HaeII r_GCGC'y
Cuts at: 1531 1779 2801 3780 1531
Size: 248 1022 979 1848
Fragments arranged by size:
1848 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 522 554 1939 1969 1971 2001 522
Size: 32 1385 30 2 30 2618
Fragments arranged by size:
2618 1385 32 30 30 2
HincII GTy'rAC
Cuts at: 1136 1136
Size: 4097
KasI G'GCGC_C
Cuts at: 1527 1775 1527
Size: 248 3849
KpnI G_GTAC'C
Cuts at: 1117 1117
Size: 4097
MluI A'CGCG_T
Cuts at: 3530 3530
Size: 4097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2768 2952 2768
Size: 184 3913
MscI TGG'CCA
Cuts at: 1858 1858
Size: 4097
MslI CAynn'nnrTG
Cuts at: 2086 2213 2086
Size: 127 3970
MspA1I CmG'CkG
Cuts at: 337 1208 1522 1882 2895 3140 337
Size: 871 314 360 1013 245 1294
Fragments arranged by size:
1294 1013 871 360 314 245
NaeI GCC'GGC
Cuts at: 2278 2278
Size: 4097
NarI GG'CG_CC
Cuts at: 1528 1776 1528
Size: 248 3849
NcoI C'CATG_G
Cuts at: 107 107
Size: 4097
NgoMIV G'CCGG_C
Cuts at: 2276 2276
Size: 4097
NspI r_CATG'y
Cuts at: 1150 1650 2557 1150
Size: 500 907 2690
Fragments arranged by size:
2690 907 500
PciI A'CATG_T
Cuts at: 2553 2553
Size: 4097
PmeI GTTT'AAAC
Cuts at: 1096 1096
Size: 4097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 4065
PpuMI rG'GwC_Cy
Cuts at: 701 701
Size: 4097
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 864 896 864
Size: 32 4065
PstI C_TGCA'G
Cuts at: 342 1007 1144 1829 342
Size: 665 137 685 2610
Fragments arranged by size:
2610 685 665 137
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4097
PvuII CAG'CTG
Cuts at: 337 1522 1882 337
Size: 1185 360 2552
Fragments arranged by size:
2552 1185 360
RsrII CG'GwC_CG
Cuts at: 2292 2292
Size: 4097
SacI G_AGCT'C
Cuts at: 1111 1111
Size: 4097
SalI G'TCGA_C
Cuts at: 1134 1134
Size: 4097
SapI GCTCTTCn'nnn_
Cuts at: 572 2120 2330 572
Size: 1548 210 2339
Fragments arranged by size:
2339 1548 210
SbfI CC_TGCA'GG
Cuts at: 1144 1144
Size: 4097
ScaI AGT'ACT
Cuts at: 3614 3614
Size: 4097
SexAI A'CCwGG_T
Cuts at: 697 697
Size: 4097
SfcI C'TryA_G
Cuts at: 33 338 1003 1140 1825 2818 3009 33
Size: 305 665 137 685 993 191 1121
Fragments arranged by size:
1121 993 685 665 305 191 137
SfoI GGC'GCC
Cuts at: 1529 1777 1529
Size: 248 3849
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
SmaI CCC'GGG
Cuts at: 1119 1119
Size: 4097
SmlI C'TyrA_G
Cuts at: 541 1038 1252 2167 2659 2921 541
Size: 497 214 915 492 262 1717
Fragments arranged by size:
1717 915 497 492 262 214
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4097
SphI G_CATG'C
Cuts at: 1150 1650 1150
Size: 500 3597
StyI C'CwwG_G
Cuts at: 107 1231 1585 107
Size: 1124 354 2619
Fragments arranged by size:
2619 1124 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1680 1813 2272 2481 1680
Size: 133 459 209 3296
Fragments arranged by size:
3296 459 209 133
TatI w'GTAC_w
Cuts at: 3612 3612
Size: 4097
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3614 3646 3614
Size: 32 4065
Tth111I GACn'n_nGTC
Cuts at: 699 1894 699
Size: 1195 2902
XbaI T'CTAG_A
Cuts at: 954 1128 954
Size: 174 3923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1592 1592
Size: 4097
XmaI C'CCGG_G
Cuts at: 1117 1117
Size: 4097
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI