Restriction map for
pF1KB8638
(Circular) (Six-base) MAPSORT of: pF1KB8638.seq Check: 8024 from: 1 to: 4235
pF1KB8638 4235 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1273 1273
Size: 4235
Acc65I G'GTAC_C
Cuts at: 1251 1251
Size: 4235
AclI AA'CG_TT
Cuts at: 4075 4075
Size: 4235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 872 1163 1240 2060 2492 2845 3507 872
Size: 291 77 820 432 353 662 1600
Fragments arranged by size:
1600 820 662 432 353 291 77
AfeI AGC'GCT
Cuts at: 958 958
Size: 4235
AflIII A'CryG_T
Cuts at: 209 1587 3387 3668 209
Size: 1378 1800 281 776
Fragments arranged by size:
1800 1378 776 281
AlwNI CAG_nnn'CTG
Cuts at: 937 1540 2978 937
Size: 603 1438 2194
Fragments arranged by size:
2194 1438 603
ApaLI G'TGCA_C
Cuts at: 3073 3073
Size: 4235
ApoI r'AATT_y
Cuts at: 199 409 415 511 693 736 1019 1239 3588
Size: 210 6 96 182 43 283 220 2349
Cuts at: 3588 3844 199
Size: 256 590
Fragments arranged by size:
2349 590 283 256 220 210 182 96
43 6
AseI AT'TA_AT
Cuts at: 20 20
Size: 4235
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4235
AvaI C'yCGr_G
Cuts at: 1255 1255
Size: 4235
BamHI G'GATC_C
Cuts at: 1260 1260
Size: 4235
BanI G'GyrC_C
Cuts at: 1251 1665 1913 1948 1251
Size: 414 248 35 3538
Fragments arranged by size:
3538 414 248 35
BanII G_rGCy'C
Cuts at: 1249 2279 1249
Size: 1030 3205
BbeI G_GCGC'C
Cuts at: 1669 1917 1669
Size: 248 3987
BbvCI CC'TCA_GC
Cuts at: 1157 1157
Size: 4235
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 128 162 128
Size: 34 4201
BciVI GTATCCnnnnn_n'
Cuts at: 2133 3189 4235 2133
Size: 1056 1046 2133
Fragments arranged by size:
2133 1056 1046
BclI T'GATC_A
Cuts at: 1755 1755
Size: 4235
BglII A'GATC_T
Cuts at: 1750 3787 1750
Size: 2037 2198
BlpI GC'TnA_GC
Cuts at: 1347 1347
Size: 4235
Bme1580I G_kGCm'C
Cuts at: 1192 1860 1953 3077 1192
Size: 668 93 1124 2350
Fragments arranged by size:
2350 1124 668 93
BmrI ACTGGGnnnn_n'
Cuts at: 507 1447 1528 1863 4007 507
Size: 940 81 335 2144 735
Fragments arranged by size:
2144 940 735 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2602 2602
Size: 4235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 973 973
Size: 4235
Bpu10I CC'TnA_GC
Cuts at: 1157 1157
Size: 4235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1411 2290 3004 3302 1411
Size: 879 714 298 2344
Fragments arranged by size:
2344 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1102 3939 1102
Size: 2837 1398
BsaAI yAC'GTr
Cuts at: 1588 1588
Size: 4235
BsaBI GATnn'nnATC
Cuts at: 6 1749 3786 6
Size: 1743 2037 455
Fragments arranged by size:
2037 1743 455
BsaHI Gr'CG_yC
Cuts at: 1666 1914 2616 4112 1666
Size: 248 702 1496 1789
Fragments arranged by size:
1789 1496 702 248
BsaWI w'CCGG_w
Cuts at: 774 1424 1945 2623 3034 3181 3455 774
Size: 650 521 678 411 147 274 1554
Fragments arranged by size:
1554 678 650 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 823 823
Size: 4235
BseYI C'CCAG_C
Cuts at: 1660 2418 3083 3491 1660
Size: 758 665 408 2404
Fragments arranged by size:
2404 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1067 1067
Size: 4235
BsiEI CG_ry'CG
Cuts at: 104 1777 1823 3053 104
Size: 1673 46 1230 1286
Fragments arranged by size:
1673 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1249 2027 2217 3077 1249
Size: 778 190 860 2407
Fragments arranged by size:
2407 860 778 190
BsmI GAATG_Cn'
Cuts at: 435 435
Size: 4235
Bsp1286I G_dGCh'C
Cuts at: 1192 1249 1860 1953 2027 2217 2279 3077 1192
Size: 57 611 93 74 190 62 798 2350
Fragments arranged by size:
2350 798 611 190 93 74 62 57
BspHI T'CATG_A
Cuts at: 2667 2667
Size: 4235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1285 1499 1801 2182 1285
Size: 214 302 381 3338
Fragments arranged by size:
3338 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1169 2527 2581 4065 4230 50
Size: 1119 1358 54 1484 165 55
Fragments arranged by size:
1484 1358 1119 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2147 2147
Size: 4235
BsrFI r'CCGG_y
Cuts at: 659 727 2233 2414 659
Size: 68 1506 181 2480
Fragments arranged by size:
2480 1506 181 68
BssSI C'ACGA_G
Cuts at: 2506 3214 2506
Size: 708 3527
BstAPI GCAn_nnn'nTGC
Cuts at: 1612 1612
Size: 4235
BstBI TT'CG_AA
Cuts at: 2596 2596
Size: 4235
BstXI CCAn_nnnn'nTGG
Cuts at: 456 639 456
Size: 183 4052
BstYI r'GATC_y
Cuts at: 1 1260 1727 1750 2085 2331 2637 2649 2735
Size: 1259 467 23 335 246 306 12 86
Cuts at: 2735 3787 1
Size: 1052 449
Fragments arranged by size:
1259 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 1001 1001
Size: 4235
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2185 2366 2529 2185
Size: 181 163 3891
Fragments arranged by size:
3891 181 163
BtsI GCAGTG_nn'
Cuts at: 1076 1478 1076
Size: 402 3833
DraI TTT'AAA
Cuts at: 1234 1234
Size: 4235
DraIII CAC_nnn'GTG
Cuts at: 1152 1152
Size: 4235
DrdI GACnn_nn'nnGTC
Cuts at: 1941 3285 1941
Size: 1344 2891
EaeI y'GGCC_r
Cuts at: 621 729 1031 1820 1994 2385 2412 3757 621
Size: 108 302 789 174 391 27 1345 1099
Fragments arranged by size:
1345 1099 789 391 302 174 108 27
EagI C'GGCC_G
Cuts at: 1820 1820
Size: 4235
EarI CTCTTCn'nnn_
Cuts at: 583 2258 2468 583
Size: 1675 210 2350
Fragments arranged by size:
2350 1675 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2443 3185 3331 4045 2443
Size: 742 146 714 2633
Fragments arranged by size:
2633 742 714 146
EcoICRI GAG'CTC
Cuts at: 1247 1247
Size: 4235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 872 973 1163 1240 2060 2492 2845 3507 872
Size: 101 190 77 820 432 353 662 1600
Fragments arranged by size:
1600 820 662 432 353 190 101 77
EcoO109I rG'GnC_Cy
Cuts at: 1201 1374 1201
Size: 173 4062
EcoRI G'AATT_C
Cuts at: 693 736 1239 693
Size: 43 503 3689
Fragments arranged by size:
3689 503 43
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 774 2254 774
Size: 1480 2755
FspI TGC'GCA
Cuts at: 2016 2016
Size: 4235
HaeII r_GCGC'y
Cuts at: 960 1669 1917 3147 3918 960
Size: 709 248 1230 771 1277
Fragments arranged by size:
1277 1230 771 709 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 343 375 818 850 2077 2107 2109 2139 343
Size: 32 443 32 1227 30 2 30 2439
Fragments arranged by size:
2439 1227 443 32 32 30 30 2
HincII GTy'rAC
Cuts at: 466 612 1274 466
Size: 146 662 3427
Fragments arranged by size:
3427 662 146
HindIII A'AGCT_T
Cuts at: 568 568
Size: 4235
KasI G'GCGC_C
Cuts at: 1665 1913 1665
Size: 248 3987
KpnI G_GTAC'C
Cuts at: 1255 1255
Size: 4235
MluI A'CGCG_T
Cuts at: 3668 3668
Size: 4235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2994 3178 2994
Size: 184 4051
MscI TGG'CCA
Cuts at: 623 1996 623
Size: 1373 2862
MslI CAynn'nnrTG
Cuts at: 637 2224 2351 637
Size: 1587 127 2521
Fragments arranged by size:
2521 1587 127
MspA1I CmG'CkG
Cuts at: 383 934 1346 1660 2020 2804 3049 383
Size: 551 412 314 360 784 245 1569
Fragments arranged by size:
1569 784 551 412 360 314 245
NaeI GCC'GGC
Cuts at: 661 729 2416 661
Size: 68 1687 2480
Fragments arranged by size:
2480 1687 68
NarI GG'CG_CC
Cuts at: 1666 1914 1666
Size: 248 3987
NgoMIV G'CCGG_C
Cuts at: 659 727 2414 659
Size: 68 1687 2480
Fragments arranged by size:
2480 1687 68
NspI r_CATG'y
Cuts at: 213 1288 1788 3391 213
Size: 1075 500 1603 1057
Fragments arranged by size:
1603 1075 1057 500
PciI A'CATG_T
Cuts at: 209 3387 209
Size: 3178 1057
PflMI CCAn_nnn'nTGG
Cuts at: 1152 1152
Size: 4235
PmeI GTTT'AAAC
Cuts at: 1234 1234
Size: 4235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2681 2713 2681
Size: 32 4203
PpuMI rG'GwC_Cy
Cuts at: 1201 1201
Size: 4235
PstI C_TGCA'G
Cuts at: 991 1017 1282 1967 991
Size: 26 265 685 3259
Fragments arranged by size:
3259 685 265 26
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4235
PvuII CAG'CTG
Cuts at: 383 934 1660 2020 383
Size: 551 726 360 2598
Fragments arranged by size:
2598 726 551 360
RsrII CG'GwC_CG
Cuts at: 2430 2430
Size: 4235
SacI G_AGCT'C
Cuts at: 1249 1249
Size: 4235
SalI G'TCGA_C
Cuts at: 1272 1272
Size: 4235
SanDI GG'GwC_CC
Cuts at: 1201 1201
Size: 4235
SapI GCTCTTCn'nnn_
Cuts at: 2258 2468 2258
Size: 210 4025
SbfI CC_TGCA'GG
Cuts at: 1282 1282
Size: 4235
ScaI AGT'ACT
Cuts at: 3752 3752
Size: 4235
SfcI C'TryA_G
Cuts at: 33 987 1013 1278 1963 2931 3122 33
Size: 954 26 265 685 968 191 1146
Fragments arranged by size:
1146 968 954 685 265 191 26
SfoI GGC'GCC
Cuts at: 1667 1915 1667
Size: 248 3987
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4235
SmaI CCC'GGG
Cuts at: 1257 1257
Size: 4235
SmlI C'TyrA_G
Cuts at: 1390 2305 3019 3281 1390
Size: 915 714 262 2344
Fragments arranged by size:
2344 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4235
SphI G_CATG'C
Cuts at: 1288 1788 1288
Size: 500 3735
StyI C'CwwG_G
Cuts at: 434 1369 1723 434
Size: 935 354 2946
Fragments arranged by size:
2946 935 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 241 1106 1818 1951 2410 2619 241
Size: 865 712 133 459 209 1857
Fragments arranged by size:
1857 865 712 459 209 133
TatI w'GTAC_w
Cuts at: 3750 3750
Size: 4235
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 856 888 3752 3784 856
Size: 32 2864 32 1307
Fragments arranged by size:
2864 1307 32 32
Tth111I GACn'n_nGTC
Cuts at: 2032 2032
Size: 4235
XbaI T'CTAG_A
Cuts at: 1266 1266
Size: 4235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1730 1730
Size: 4235
XmaI C'CCGG_G
Cuts at: 1255 1255
Size: 4235
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmBI BspEI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI