Restriction map for
pF1KB8636
(Circular) (Six-base) MAPSORT of: pF1KB8636.seq Check: 1744 from: 1 to: 4118
pF1KB8636 4118 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1156 1156
Size: 4118
Acc65I G'GTAC_C
Cuts at: 1134 1134
Size: 4118
AclI AA'CG_TT
Cuts at: 3958 3958
Size: 4118
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 484 915 1000 1943 2375 2728 3390 214
Size: 270 431 85 943 432 353 662 942
Fragments arranged by size:
943 942 662 432 431 353 270 85
AflIII A'CryG_T
Cuts at: 1470 3270 3551 1470
Size: 1800 281 2037
Fragments arranged by size:
2037 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 507 1423 2861 507
Size: 916 1438 1764
Fragments arranged by size:
1764 1438 916
ApaLI G'TGCA_C
Cuts at: 2956 2956
Size: 4118
ApoI r'AATT_y
Cuts at: 208 398 406 1122 3471 3727 208
Size: 190 8 716 2349 256 599
Fragments arranged by size:
2349 716 599 256 190 8
AseI AT'TA_AT
Cuts at: 20 20
Size: 4118
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
AvaI C'yCGr_G
Cuts at: 1138 1138
Size: 4118
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 842 875 842
Size: 33 4085
BamHI G'GATC_C
Cuts at: 1143 1143
Size: 4118
BanI G'GyrC_C
Cuts at: 1134 1548 1796 1831 1134
Size: 414 248 35 3421
Fragments arranged by size:
3421 414 248 35
BanII G_rGCy'C
Cuts at: 623 1132 2162 623
Size: 509 1030 2579
Fragments arranged by size:
2579 1030 509
BbeI G_GCGC'C
Cuts at: 1552 1800 1552
Size: 248 3870
BbsI GAAGACnn'nnnn_
Cuts at: 194 331 194
Size: 137 3981
BciVI GTATCCnnnnn_n'
Cuts at: 2016 3072 4118 2016
Size: 1056 1046 2016
Fragments arranged by size:
2016 1056 1046
BclI T'GATC_A
Cuts at: 1638 1638
Size: 4118
BfrBI ATG'CAT
Cuts at: 896 896
Size: 4118
BglII A'GATC_T
Cuts at: 1633 3670 1633
Size: 2037 2081
BlpI GC'TnA_GC
Cuts at: 622 1230 622
Size: 608 3510
Bme1580I G_kGCm'C
Cuts at: 1743 1836 2960 1743
Size: 93 1124 2901
Fragments arranged by size:
2901 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 140 965 1330 1411 1746 3890 140
Size: 825 365 81 335 2144 368
Fragments arranged by size:
2144 825 368 365 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2485 2485
Size: 4118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 1294 2173 2887 3185 730
Size: 564 879 714 298 1663
Fragments arranged by size:
1663 879 714 564 298
BsaI GGTCTCn'nnnn_
Cuts at: 3822 3822
Size: 4118
BsaAI yAC'GTr
Cuts at: 1471 1471
Size: 4118
BsaBI GATnn'nnATC
Cuts at: 6 1632 3669 6
Size: 1626 2037 455
Fragments arranged by size:
2037 1626 455
BsaHI Gr'CG_yC
Cuts at: 1549 1797 2499 3995 1549
Size: 248 702 1496 1672
Fragments arranged by size:
1672 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1307 1828 2506 2917 3064 3338 1307
Size: 521 678 411 147 274 2087
Fragments arranged by size:
2087 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1543 2301 2966 3374 1543
Size: 758 665 408 2287
Fragments arranged by size:
2287 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1660 1706 2936 104
Size: 1556 46 1230 1286
Fragments arranged by size:
1556 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 623 1132 1910 2100 2960 623
Size: 509 778 190 860 1781
Fragments arranged by size:
1781 860 778 509 190
BsmI GAATG_Cn'
Cuts at: 773 773
Size: 4118
Bsp1286I G_dGCh'C
Cuts at: 623 1132 1743 1836 1910 2100 2162 2960 623
Size: 509 611 93 74 190 62 798 1781
Fragments arranged by size:
1781 798 611 509 190 93 74 62
BspHI T'CATG_A
Cuts at: 2550 2550
Size: 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1168 1382 1684 2065 1168
Size: 214 302 381 3221
Fragments arranged by size:
3221 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2410 2464 3948 4113 50
Size: 2360 54 1484 165 55
Fragments arranged by size:
2360 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2030 2030
Size: 4118
BsrFI r'CCGG_y
Cuts at: 2116 2297 2116
Size: 181 3937
BssSI C'ACGA_G
Cuts at: 2389 3097 2389
Size: 708 3410
BstAPI GCAn_nnn'nTGC
Cuts at: 1495 1495
Size: 4118
BstBI TT'CG_AA
Cuts at: 2479 2479
Size: 4118
BstEII G'GTnAC_C
Cuts at: 273 273
Size: 4118
BstYI r'GATC_y
Cuts at: 1 230 1143 1610 1633 1968 2214 2520 2532
Size: 229 913 467 23 335 246 306 12
Cuts at: 2532 2618 3670 1
Size: 86 1052 449
Fragments arranged by size:
1052 913 467 449 335 306 246 229
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2068 2249 2412 2068
Size: 181 163 3774
Fragments arranged by size:
3774 181 163
BtsI GCAGTG_nn'
Cuts at: 1361 1361
Size: 4118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 932 967 932
Size: 35 4083
DraI TTT'AAA
Cuts at: 1117 1117
Size: 4118
DrdI GACnn_nn'nnGTC
Cuts at: 1824 3168 1824
Size: 1344 2774
EaeI y'GGCC_r
Cuts at: 1703 1877 2268 2295 3640 1703
Size: 174 391 27 1345 2181
Fragments arranged by size:
2181 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1703 1703
Size: 4118
EarI CTCTTCn'nnn_
Cuts at: 285 986 1047 2141 2351 285
Size: 701 61 1094 210 2052
Fragments arranged by size:
2052 1094 701 210 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2326 3068 3214 3928 2326
Size: 742 146 714 2516
Fragments arranged by size:
2516 742 714 146
EcoICRI GAG'CTC
Cuts at: 621 1130 621
Size: 509 3609
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 484 915 1000 1943 2375 2728 3390 214
Size: 270 431 85 943 432 353 662 942
Fragments arranged by size:
943 942 662 432 431 353 270 85
EcoO109I rG'GnC_Cy
Cuts at: 1257 1257
Size: 4118
EcoRI G'AATT_C
Cuts at: 406 1122 406
Size: 716 3402
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 979 2137 979
Size: 1158 2960
FspI TGC'GCA
Cuts at: 1899 1899
Size: 4118
HaeII r_GCGC'y
Cuts at: 1552 1800 3030 3801 1552
Size: 248 1230 771 1869
Fragments arranged by size:
1869 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 257 289 1960 1990 1992 2022 257
Size: 32 1671 30 2 30 2353
Fragments arranged by size:
2353 1671 32 30 30 2
HincII GTy'rAC
Cuts at: 591 927 1071 1157 591
Size: 336 144 86 3552
Fragments arranged by size:
3552 336 144 86
HindIII A'AGCT_T
Cuts at: 249 249
Size: 4118
HpaI GTT'AAC
Cuts at: 591 591
Size: 4118
KasI G'GCGC_C
Cuts at: 1548 1796 1548
Size: 248 3870
KpnI G_GTAC'C
Cuts at: 1138 1138
Size: 4118
MluI A'CGCG_T
Cuts at: 3551 3551
Size: 4118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2877 3061 2877
Size: 184 3934
MscI TGG'CCA
Cuts at: 1879 1879
Size: 4118
MslI CAynn'nnrTG
Cuts at: 2107 2234 2107
Size: 127 3991
MspA1I CmG'CkG
Cuts at: 1229 1543 1903 2687 2932 1229
Size: 314 360 784 245 2415
Fragments arranged by size:
2415 784 360 314 245
NaeI GCC'GGC
Cuts at: 2299 2299
Size: 4118
NarI GG'CG_CC
Cuts at: 1549 1797 1549
Size: 248 3870
NgoMIV G'CCGG_C
Cuts at: 2297 2297
Size: 4118
NsiI A_TGCA'T
Cuts at: 898 898
Size: 4118
NspI r_CATG'y
Cuts at: 702 815 1171 1671 3274 702
Size: 113 356 500 1603 1546
Fragments arranged by size:
1603 1546 500 356 113
PciI A'CATG_T
Cuts at: 3270 3270
Size: 4118
PflMI CCAn_nnn'nTGG
Cuts at: 481 481
Size: 4118
PmeI GTTT'AAAC
Cuts at: 1117 1117
Size: 4118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 497 529 2564 2596 497
Size: 32 2035 32 2019
Fragments arranged by size:
2035 2019 32 32
PstI C_TGCA'G
Cuts at: 1165 1850 1165
Size: 685 3433
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4118
PvuII CAG'CTG
Cuts at: 1543 1903 1543
Size: 360 3758
RsrII CG'GwC_CG
Cuts at: 2313 2313
Size: 4118
SacI G_AGCT'C
Cuts at: 623 1132 623
Size: 509 3609
SalI G'TCGA_C
Cuts at: 1155 1155
Size: 4118
SapI GCTCTTCn'nnn_
Cuts at: 1047 2141 2351 1047
Size: 1094 210 2814
Fragments arranged by size:
2814 1094 210
SbfI CC_TGCA'GG
Cuts at: 1165 1165
Size: 4118
ScaI AGT'ACT
Cuts at: 3635 3635
Size: 4118
SfcI C'TryA_G
Cuts at: 33 361 758 1161 1846 2814 3005 33
Size: 328 397 403 685 968 191 1146
Fragments arranged by size:
1146 968 685 403 397 328 191
SfoI GGC'GCC
Cuts at: 1550 1798 1550
Size: 248 3870
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
SmaI CCC'GGG
Cuts at: 1140 1140
Size: 4118
SmlI C'TyrA_G
Cuts at: 709 1273 2188 2902 3164 709
Size: 564 915 714 262 1663
Fragments arranged by size:
1663 915 714 564 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4118
SphI G_CATG'C
Cuts at: 1171 1671 1171
Size: 500 3618
StyI C'CwwG_G
Cuts at: 542 1252 1606 542
Size: 710 354 3054
Fragments arranged by size:
3054 710 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1701 1834 2293 2502 1701
Size: 133 459 209 3317
Fragments arranged by size:
3317 459 209 133
TatI w'GTAC_w
Cuts at: 127 3633 127
Size: 3506 612
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4086
Tth111I GACn'n_nGTC
Cuts at: 1915 1915
Size: 4118
XbaI T'CTAG_A
Cuts at: 1149 1149
Size: 4118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1613 1613
Size: 4118
XmaI C'CCGG_G
Cuts at: 1138 1138
Size: 4118
XmnI GAAnn'nnTTC
Cuts at: 509 746 509
Size: 237 3881
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BbvCI BcgI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI
BspEI BsrGI BssHII BstXI BstZ17I Bsu36I BtgI ClaI
DraIII EcoNI EcoRV FseI FspAI MfeI NcoI NdeI
NheI NotI NruI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI