Restriction map for
pF1KB8631
(Circular) (Six-base) MAPSORT of: pF1KB8631.seq Check: 3263 from: 1 to: 4505
pF1KB8631 4505 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1488 1488
Size: 4505
AccI GT'mk_AC
Cuts at: 596 1028 1221 1543 596
Size: 432 193 322 3558
Fragments arranged by size:
3558 432 322 193
Acc65I G'GTAC_C
Cuts at: 1521 1521
Size: 4505
AclI AA'CG_TT
Cuts at: 4345 4345
Size: 4505
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 710 761 1043 1135 2330 2762 3115 3777
Size: 459 51 282 92 1195 432 353 662
Cuts at: 3777 251
Size: 979
Fragments arranged by size:
1195 979 662 459 432 353 282 92
51
AflIII A'CryG_T
Cuts at: 1857 3657 3938 1857
Size: 1800 281 2424
Fragments arranged by size:
2424 1800 281
AleI CACnn'nnGTG
Cuts at: 715 715
Size: 4505
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 258 1296 258
Size: 1038 3467
AlwNI CAG_nnn'CTG
Cuts at: 1810 3248 1810
Size: 1438 3067
ApaLI G'TGCA_C
Cuts at: 3343 3343
Size: 4505
ApoI r'AATT_y
Cuts at: 746 1509 3858 4114 746
Size: 763 2349 256 1137
Fragments arranged by size:
2349 1137 763 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4505
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4505
AvaI C'yCGr_G
Cuts at: 284 1525 284
Size: 1241 3264
BamHI G'GATC_C
Cuts at: 333 1530 333
Size: 1197 3308
BanI G'GyrC_C
Cuts at: 1521 1935 2183 2218 1521
Size: 414 248 35 3808
Fragments arranged by size:
3808 414 248 35
BanII G_rGCy'C
Cuts at: 197 321 494 1519 2549 197
Size: 124 173 1025 1030 2153
Fragments arranged by size:
2153 1030 1025 173 124
BbeI G_GCGC'C
Cuts at: 1939 2187 1939
Size: 248 4257
BbsI GAAGACnn'nnnn_
Cuts at: 201 201
Size: 4505
BbvCI CC'TCA_GC
Cuts at: 1415 1415
Size: 4505
BciVI GTATCCnnnnn_n'
Cuts at: 2403 3459 4505 2403
Size: 1056 1046 2403
Fragments arranged by size:
2403 1056 1046
BclI T'GATC_A
Cuts at: 2025 2025
Size: 4505
BglII A'GATC_T
Cuts at: 2020 4057 2020
Size: 2037 2468
BlpI GC'TnA_GC
Cuts at: 1617 1617
Size: 4505
Bme1580I G_kGCm'C
Cuts at: 2130 2223 3347 2130
Size: 93 1124 3288
Fragments arranged by size:
3288 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 678 1717 1798 2133 4277 678
Size: 1039 81 335 2144 906
Fragments arranged by size:
2144 1039 906 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2872 2872
Size: 4505
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 434
Size: 4505
Bpu10I CC'TnA_GC
Cuts at: 1415 1415
Size: 4505
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 1179 1681 2560 3274 3572 595
Size: 584 502 879 714 298 1528
Fragments arranged by size:
1528 879 714 584 502 298
BsaI GGTCTCn'nnnn_
Cuts at: 106 886 4209 106
Size: 780 3323 402
Fragments arranged by size:
3323 780 402
BsaAI yAC'GTr
Cuts at: 1206 1858 1206
Size: 652 3853
BsaBI GATnn'nnATC
Cuts at: 6 2019 4056 6
Size: 2013 2037 455
Fragments arranged by size:
2037 2013 455
BsaHI Gr'CG_yC
Cuts at: 1936 2184 2886 4382 1936
Size: 248 702 1496 2059
Fragments arranged by size:
2059 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1694 2215 2893 3304 3451 3725 1694
Size: 521 678 411 147 274 2474
Fragments arranged by size:
2474 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 179 209 378 408 1163 1193 179
Size: 30 169 30 755 30 3491
Fragments arranged by size:
3491 755 169 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 667 667
Size: 4505
BseYI C'CCAG_C
Cuts at: 158 179 324 1930 2688 3353 3761 158
Size: 21 145 1606 758 665 408 902
Fragments arranged by size:
1606 902 758 665 408 145 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 607
Size: 4505
BsiEI CG_ry'CG
Cuts at: 104 2047 2093 3323 104
Size: 1943 46 1230 1286
Fragments arranged by size:
1943 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1519 2297 2487 3347 1519
Size: 778 190 860 2677
Fragments arranged by size:
2677 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 197 321 494 1519 2130 2223 2297 2487 2549
Size: 124 173 1025 611 93 74 190 62
Cuts at: 2549 3347 197
Size: 798 1355
Fragments arranged by size:
1355 1025 798 611 190 173 124 93
74 62
BspHI T'CATG_A
Cuts at: 2937 2937
Size: 4505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 629 1488 1555 1769 2071 2452 629
Size: 859 67 214 302 381 2682
Fragments arranged by size:
2682 859 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 2797 2851 4335 4500 50
Size: 2747 54 1484 165 55
Fragments arranged by size:
2747 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 242 2417 242
Size: 2175 2330
BsrFI r'CCGG_y
Cuts at: 115 601 2503 2684 115
Size: 486 1902 181 1936
Fragments arranged by size:
1936 1902 486 181
BssSI C'ACGA_G
Cuts at: 2776 3484 2776
Size: 708 3797
BstAPI GCAn_nnn'nTGC
Cuts at: 630 1882 630
Size: 1252 3253
BstBI TT'CG_AA
Cuts at: 997 2866 997
Size: 1869 2636
BstYI r'GATC_y
Cuts at: 1 142 333 1233 1530 1997 2020 2355 2601
Size: 141 191 900 297 467 23 335 246
Cuts at: 2601 2907 2919 3005 4057 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 900 467 449 335 306 297 246
191 141 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 760 760
Size: 4505
BtgI C'CryG_G
Cuts at: 107 221 107
Size: 114 4391
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2455 2636 2799 2455
Size: 181 163 4161
Fragments arranged by size:
4161 181 163
BtsI GCAGTG_nn'
Cuts at: 240 1748 240
Size: 1508 2997
DraI TTT'AAA
Cuts at: 1504 1504
Size: 4505
DrdI GACnn_nn'nnGTC
Cuts at: 481 1036 2211 3555 481
Size: 555 1175 1344 1431
Fragments arranged by size:
1431 1344 1175 555
EaeI y'GGCC_r
Cuts at: 396 1315 2090 2264 2655 2682 4027 396
Size: 919 775 174 391 27 1345 874
Fragments arranged by size:
1345 919 874 775 391 174 27
EagI C'GGCC_G
Cuts at: 2090 2090
Size: 4505
EarI CTCTTCn'nnn_
Cuts at: 161 1001 2528 2738 161
Size: 840 1527 210 1928
Fragments arranged by size:
1928 1527 840 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2713 3455 3601 4315 2713
Size: 742 146 714 2903
Fragments arranged by size:
2903 742 714 146
EcoICRI GAG'CTC
Cuts at: 1517 1517
Size: 4505
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 434 710 761 1043 1135 2330 2762 3115
Size: 183 276 51 282 92 1195 432 353
Cuts at: 3115 3777 251
Size: 662 979
Fragments arranged by size:
1195 979 662 432 353 282 276 183
92 51
EcoO109I rG'GnC_Cy
Cuts at: 289 389 1644 289
Size: 100 1255 3150
Fragments arranged by size:
3150 1255 100
EcoRI G'AATT_C
Cuts at: 1509 1509
Size: 4505
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1411 2524 1411
Size: 1113 3392
FspI TGC'GCA
Cuts at: 835 2286 835
Size: 1451 3054
FspAI rTGC'GCAy
Cuts at: 835 835
Size: 4505
HaeII r_GCGC'y
Cuts at: 1939 2187 3417 4188 1939
Size: 248 1230 771 2256
Fragments arranged by size:
2256 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 179 211 538 570 885 917 1002 1034 1163
Size: 32 327 32 315 32 85 32 129
Cuts at: 1163 1195 2347 2377 2379 2409 179
Size: 32 1152 30 2 30 2275
Fragments arranged by size:
2275 1152 327 315 129 85 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 912 1544 912
Size: 632 3873
KasI G'GCGC_C
Cuts at: 1935 2183 1935
Size: 248 4257
KpnI G_GTAC'C
Cuts at: 1525 1525
Size: 4505
MluI A'CGCG_T
Cuts at: 3938 3938
Size: 4505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3264 3448 3264
Size: 184 4321
MscI TGG'CCA
Cuts at: 398 1317 2266 398
Size: 919 949 2637
Fragments arranged by size:
2637 949 919
MslI CAynn'nnrTG
Cuts at: 517 715 2494 2621 517
Size: 198 1779 127 2401
Fragments arranged by size:
2401 1779 198 127
MspA1I CmG'CkG
Cuts at: 179 324 454 551 1616 1930 2290 3074 3319
Size: 145 130 97 1065 314 360 784 245
Cuts at: 3319 179
Size: 1365
Fragments arranged by size:
1365 1065 784 360 314 245 145 130
97
NaeI GCC'GGC
Cuts at: 2686 2686
Size: 4505
NarI GG'CG_CC
Cuts at: 1936 2184 1936
Size: 248 4257
NcoI C'CATG_G
Cuts at: 107 107
Size: 4505
NgoMIV G'CCGG_C
Cuts at: 2684 2684
Size: 4505
NspI r_CATG'y
Cuts at: 834 1558 2058 3661 834
Size: 724 500 1603 1678
Fragments arranged by size:
1678 1603 724 500
PciI A'CATG_T
Cuts at: 3657 3657
Size: 4505
PmeI GTTT'AAAC
Cuts at: 1504 1504
Size: 4505
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2951 2983 2951
Size: 32 4473
PpuMI rG'GwC_Cy
Cuts at: 289 389 289
Size: 100 4405
PstI C_TGCA'G
Cuts at: 247 965 1272 1485 1552 2237 247
Size: 718 307 213 67 685 2515
Fragments arranged by size:
2515 718 685 307 213 67
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4505
PvuII CAG'CTG
Cuts at: 179 454 551 1930 2290 179
Size: 275 97 1379 360 2394
Fragments arranged by size:
2394 1379 360 275 97
RsrII CG'GwC_CG
Cuts at: 2700 2700
Size: 4505
SacI G_AGCT'C
Cuts at: 1519 1519
Size: 4505
SalI G'TCGA_C
Cuts at: 1542 1542
Size: 4505
SapI GCTCTTCn'nnn_
Cuts at: 2528 2738 2528
Size: 210 4295
SbfI CC_TGCA'GG
Cuts at: 1552 1552
Size: 4505
ScaI AGT'ACT
Cuts at: 1156 4022 1156
Size: 2866 1639
SfcI C'TryA_G
Cuts at: 33 243 275 961 1268 1481 1548 2233 3201
Size: 210 32 686 307 213 67 685 968
Cuts at: 3201 3392 33
Size: 191 1146
Fragments arranged by size:
1146 968 686 685 307 213 210 191
67 32
SfoI GGC'GCC
Cuts at: 1937 2185 1937
Size: 248 4257
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4505
SmaI CCC'GGG
Cuts at: 1527 1527
Size: 4505
SmlI C'TyrA_G
Cuts at: 610 1158 1660 2575 3289 3551 610
Size: 548 502 915 714 262 1564
Fragments arranged by size:
1564 915 714 548 502 262
SnaBI TAC'GTA
Cuts at: 1206 1206
Size: 4505
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4505
SphI G_CATG'C
Cuts at: 834 1558 2058 834
Size: 724 500 3281
Fragments arranged by size:
3281 724 500
StyI C'CwwG_G
Cuts at: 107 1639 1993 107
Size: 1532 354 2619
Fragments arranged by size:
2619 1532 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 546 2088 2221 2680 2889 546
Size: 1542 133 459 209 2162
Fragments arranged by size:
2162 1542 459 209 133
TatI w'GTAC_w
Cuts at: 380 1154 1436 4020 380
Size: 774 282 2584 865
Fragments arranged by size:
2584 865 774 282
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 378 410 4022 4054 378
Size: 32 3612 32 829
Fragments arranged by size:
3612 829 32 32
Tth111I GACn'n_nGTC
Cuts at: 2302 2302
Size: 4505
XbaI T'CTAG_A
Cuts at: 1536 1536
Size: 4505
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 321 2000 321
Size: 1679 2826
XmaI C'CCGG_G
Cuts at: 1525 1525
Size: 4505
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsmI BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI SspI StuI
SwaI XhoI XmnI ZraI