Restriction map for
pF1KB8628
(Circular) (Six-base) MAPSORT of: pF1KB8628.seq Check: 1095 from: 1 to: 5636
pF1KB8628 5636 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 876 885 2674 876
Size: 9 1789 3838
Fragments arranged by size:
3838 1789 9
Acc65I G'GTAC_C
Cuts at: 2652 2652
Size: 5636
AclI AA'CG_TT
Cuts at: 5476 5476
Size: 5636
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 929 2059 2389 2518 3461 3893 4246 4908
Size: 199 1130 330 129 943 432 353 662
Cuts at: 4908 730
Size: 1458
Fragments arranged by size:
1458 1130 943 662 432 353 330 199
129
AflIII A'CryG_T
Cuts at: 2988 4788 5069 2988
Size: 1800 281 3555
Fragments arranged by size:
3555 1800 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1458 1490 1458
Size: 32 5604
AlwNI CAG_nnn'CTG
Cuts at: 2412 2941 4379 2412
Size: 529 1438 3669
Fragments arranged by size:
3669 1438 529
ApaLI G'TGCA_C
Cuts at: 4474 4474
Size: 5636
ApoI r'AATT_y
Cuts at: 482 523 1796 2341 2640 4989 5245 482
Size: 41 1273 545 299 2349 256 873
Fragments arranged by size:
2349 1273 873 545 299 256 41
AseI AT'TA_AT
Cuts at: 20 2515 20
Size: 2495 3141
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5636
AvaI C'yCGr_G
Cuts at: 1753 2656 1753
Size: 903 4733
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1693 1726 1693
Size: 33 5603
BamHI G'GATC_C
Cuts at: 939 2661 939
Size: 1722 3914
BanI G'GyrC_C
Cuts at: 2652 3066 3314 3349 2652
Size: 414 248 35 4939
Fragments arranged by size:
4939 414 248 35
BanII G_rGCy'C
Cuts at: 2161 2487 2650 3680 2161
Size: 326 163 1030 4117
Fragments arranged by size:
4117 1030 326 163
BbeI G_GCGC'C
Cuts at: 3070 3318 3070
Size: 248 5388
BbsI GAAGACnn'nnnn_
Cuts at: 622 622
Size: 5636
BciVI GTATCCnnnnn_n'
Cuts at: 489 1694 3534 4590 5636 489
Size: 1205 1840 1056 1046 489
Fragments arranged by size:
1840 1205 1056 1046 489
BclI T'GATC_A
Cuts at: 3156 3156
Size: 5636
BglII A'GATC_T
Cuts at: 672 1176 3151 5188 672
Size: 504 1975 2037 1120
Fragments arranged by size:
2037 1975 1120 504
BlpI GC'TnA_GC
Cuts at: 2447 2748 2447
Size: 301 5335
Bme1580I G_kGCm'C
Cuts at: 3261 3354 4478 3261
Size: 93 1124 4419
Fragments arranged by size:
4419 1124 93
BmgBI CAC'GTC
Cuts at: 1010 2527 1010
Size: 1517 4119
BmrI ACTGGGnnnn_n'
Cuts at: 2422 2848 2929 3264 5408 2422
Size: 426 81 335 2144 2650
Fragments arranged by size:
2650 2144 426 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 650 1205 4003 650
Size: 555 2798 2283
Fragments arranged by size:
2798 2283 555
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 2437 2501 2638 158
Size: 2279 64 137 3156
Fragments arranged by size:
3156 2279 137 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1669 2812 3691 4405 4703 1669
Size: 1143 879 714 298 2602
Fragments arranged by size:
2602 1143 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1176 1411 5340 1176
Size: 235 3929 1472
Fragments arranged by size:
3929 1472 235
BsaAI yAC'GTr
Cuts at: 2989 2989
Size: 5636
BsaBI GATnn'nnATC
Cuts at: 6 3150 5187 6
Size: 3144 2037 455
Fragments arranged by size:
3144 2037 455
BsaHI Gr'CG_yC
Cuts at: 3067 3315 4017 5513 3067
Size: 248 702 1496 3190
Fragments arranged by size:
3190 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2825 3346 4024 4435 4582 4856 2825
Size: 521 678 411 147 274 3605
Fragments arranged by size:
3605 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1178 1507 1178
Size: 329 5307
BseYI C'CCAG_C
Cuts at: 3061 3819 4484 4892 3061
Size: 758 665 408 3805
Fragments arranged by size:
3805 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1728 1728
Size: 5636
BsiEI CG_ry'CG
Cuts at: 104 3178 3224 4454 104
Size: 3074 46 1230 1286
Fragments arranged by size:
3074 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 182 2650 3428 3618 4478 182
Size: 2468 778 190 860 1340
Fragments arranged by size:
2468 1340 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 182 2161 2487 2650 3261 3354 3428 3618 3680
Size: 1979 326 163 611 93 74 190 62
Cuts at: 3680 4478 182
Size: 798 1340
Fragments arranged by size:
1979 1340 798 611 326 190 163 93
74 62
BspHI T'CATG_A
Cuts at: 4068 4068
Size: 5636
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2686 2900 3202 3583 2686
Size: 214 302 381 4739
Fragments arranged by size:
4739 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3928 3982 5466 5631 50
Size: 3878 54 1484 165 55
Fragments arranged by size:
3878 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 697 1702 2441 3548 697
Size: 1005 739 1107 2785
Fragments arranged by size:
2785 1107 1005 739
BsrFI r'CCGG_y
Cuts at: 3634 3815 3634
Size: 181 5455
BsrGI T'GTAC_A
Cuts at: 2372 2372
Size: 5636
BssSI C'ACGA_G
Cuts at: 3907 4615 3907
Size: 708 4928
BstAPI GCAn_nnn'nTGC
Cuts at: 3013 3013
Size: 5636
BstBI TT'CG_AA
Cuts at: 3997 3997
Size: 5636
BstEII G'GTnAC_C
Cuts at: 1366 1857 1366
Size: 491 5145
BstYI r'GATC_y
Cuts at: 1 672 741 939 1176 2661 3128 3151 3486
Size: 671 69 198 237 1485 467 23 335
Cuts at: 3486 3732 4038 4050 4136 5188 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1485 1052 671 467 449 335 306 246
237 198 86 69 23 12
BstZ17I GTA'TAC
Cuts at: 877 877
Size: 5636
Bsu36I CC'TnA_GG
Cuts at: 2431 2431
Size: 5636
BtgI C'CryG_G
Cuts at: 504 2162 504
Size: 1658 3978
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3586 3767 3930 3586
Size: 181 163 5292
Fragments arranged by size:
5292 181 163
BtsI GCAGTG_nn'
Cuts at: 2879 2879
Size: 5636
DraI TTT'AAA
Cuts at: 608 867 1505 2635 608
Size: 259 638 1130 3609
Fragments arranged by size:
3609 1130 638 259
DraIII CAC_nnn'GTG
Cuts at: 2154 2154
Size: 5636
DrdI GACnn_nn'nnGTC
Cuts at: 3342 4686 3342
Size: 1344 4292
EaeI y'GGCC_r
Cuts at: 729 2091 3221 3395 3786 3813 5158 729
Size: 1362 1130 174 391 27 1345 1207
Fragments arranged by size:
1362 1345 1207 1130 391 174 27
EagI C'GGCC_G
Cuts at: 3221 3221
Size: 5636
EarI CTCTTCn'nnn_
Cuts at: 2204 2213 2486 3659 3869 2204
Size: 9 273 1173 210 3971
Fragments arranged by size:
3971 1173 273 210 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3844 4586 4732 5446 3844
Size: 742 146 714 4034
Fragments arranged by size:
4034 742 714 146
EcoICRI GAG'CTC
Cuts at: 2648 2648
Size: 5636
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 730 929 2059 2389 2437 2501 2518 2638
Size: 572 199 1130 330 48 64 17 120
Cuts at: 2638 3461 3893 4246 4908 158
Size: 823 432 353 662 886
Fragments arranged by size:
1130 886 823 662 572 432 353 330
199 120 64 48 17
EcoO109I rG'GnC_Cy
Cuts at: 1084 2775 1084
Size: 1691 3945
EcoRI G'AATT_C
Cuts at: 523 2640 523
Size: 2117 3519
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3655 3655
Size: 5636
FspI TGC'GCA
Cuts at: 3417 3417
Size: 5636
HaeII r_GCGC'y
Cuts at: 3070 3318 4548 5319 3070
Size: 248 1230 771 3387
Fragments arranged by size:
3387 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2350 2382 3478 3508 3510 3540 2350
Size: 32 1096 30 2 30 4446
Fragments arranged by size:
4446 1096 32 30 30 2
HincII GTy'rAC
Cuts at: 998 2675 998
Size: 1677 3959
HindIII A'AGCT_T
Cuts at: 315 1167 315
Size: 852 4784
KasI G'GCGC_C
Cuts at: 3066 3314 3066
Size: 248 5388
KpnI G_GTAC'C
Cuts at: 2656 2656
Size: 5636
MfeI C'AATT_G
Cuts at: 213 774 213
Size: 561 5075
MluI A'CGCG_T
Cuts at: 5069 5069
Size: 5636
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 333 731 785 1665 4395 4579 333
Size: 398 54 880 2730 184 1390
Fragments arranged by size:
2730 1390 880 398 184 54
MscI TGG'CCA
Cuts at: 2093 3397 2093
Size: 1304 4332
MslI CAynn'nnrTG
Cuts at: 350 3625 3752 350
Size: 3275 127 2234
Fragments arranged by size:
3275 2234 127
MspA1I CmG'CkG
Cuts at: 2747 3061 3421 4205 4450 2747
Size: 314 360 784 245 3933
Fragments arranged by size:
3933 784 360 314 245
NaeI GCC'GGC
Cuts at: 3817 3817
Size: 5636
NarI GG'CG_CC
Cuts at: 3067 3315 3067
Size: 248 5388
NcoI C'CATG_G
Cuts at: 504 2162 504
Size: 1658 3978
NgoMIV G'CCGG_C
Cuts at: 3815 3815
Size: 5636
NspI r_CATG'y
Cuts at: 816 996 1398 2088 2593 2689 3189 4792 816
Size: 180 402 690 505 96 500 1603 1660
Fragments arranged by size:
1660 1603 690 505 500 402 180 96
PasI CC'CwG_GG
Cuts at: 1661 1661
Size: 5636
PciI A'CATG_T
Cuts at: 4788 4788
Size: 5636
PflMI CCAn_nnn'nTGG
Cuts at: 510 2191 510
Size: 1681 3955
PfoI T'CCnGG_A
Cuts at: 1080 2478 2615 1080
Size: 1398 137 4101
Fragments arranged by size:
4101 1398 137
PmeI GTTT'AAAC
Cuts at: 2635 2635
Size: 5636
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4082 4114 4082
Size: 32 5604
PpuMI rG'GwC_Cy
Cuts at: 1084 1084
Size: 5636
PshAI GACnn'nnGTC
Cuts at: 2060 2060
Size: 5636
PstI C_TGCA'G
Cuts at: 1694 1986 2683 3368 1694
Size: 292 697 685 3962
Fragments arranged by size:
3962 697 685 292
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5636
PvuII CAG'CTG
Cuts at: 3061 3421 3061
Size: 360 5276
RsrII CG'GwC_CG
Cuts at: 3831 3831
Size: 5636
SacI G_AGCT'C
Cuts at: 2650 2650
Size: 5636
SalI G'TCGA_C
Cuts at: 2673 2673
Size: 5636
SapI GCTCTTCn'nnn_
Cuts at: 2486 3659 3869 2486
Size: 1173 210 4253
Fragments arranged by size:
4253 1173 210
SbfI CC_TGCA'GG
Cuts at: 2683 2683
Size: 5636
ScaI AGT'ACT
Cuts at: 5153 5153
Size: 5636
SexAI A'CCwGG_T
Cuts at: 1038 1679 1038
Size: 641 4995
SfcI C'TryA_G
Cuts at: 33 1690 1982 2175 2679 3364 4332 4523 33
Size: 1657 292 193 504 685 968 191 1146
Fragments arranged by size:
1657 1146 968 685 504 292 193 191
SfoI GGC'GCC
Cuts at: 3068 3316 3068
Size: 248 5388
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5636
SmaI CCC'GGG
Cuts at: 1755 2658 1755
Size: 903 4733
SmlI C'TyrA_G
Cuts at: 1648 2791 3706 4420 4682 1648
Size: 1143 915 714 262 2602
Fragments arranged by size:
2602 1143 915 714 262
SpeI A'CTAG_T
Cuts at: 67 1843 67
Size: 1776 3860
SphI G_CATG'C
Cuts at: 2689 3189 2689
Size: 500 5136
SspI AAT'ATT
Cuts at: 405 737 1227 1264 1611 1940 405
Size: 332 490 37 347 329 4101
Fragments arranged by size:
4101 490 347 332 329 37
StyI C'CwwG_G
Cuts at: 390 438 504 1401 2162 2530 2770 3124 390
Size: 48 66 897 761 368 240 354 2902
Fragments arranged by size:
2902 897 761 368 354 240 66 48
SwaI ATTT'AAAT
Cuts at: 1505 1505
Size: 5636
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3219 3352 3811 4020 3219
Size: 133 459 209 4835
Fragments arranged by size:
4835 459 209 133
TatI w'GTAC_w
Cuts at: 2078 2372 2519 5151 2078
Size: 294 147 2632 2563
Fragments arranged by size:
2632 2563 294 147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 290 322 1062 1094 5153 5185 290
Size: 32 740 32 4059 32 741
Fragments arranged by size:
4059 741 740 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1008 3433 1008
Size: 2425 3211
XbaI T'CTAG_A
Cuts at: 669 2667 669
Size: 1998 3638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3131 3131
Size: 5636
XmaI C'CCGG_G
Cuts at: 1753 2656 1753
Size: 903 4733
XmnI GAAnn'nnTTC
Cuts at: 1280 1280
Size: 5636
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
ApaI AscI AvrII BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BspEI BssHII BstXI
ClaI CspCI EcoNI EcoRV FseI FspAI HpaI NdeI
NheI NotI NruI NsiI PacI PmlI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
StuI XhoI ZraI