Restriction map for
pF1KB8625
(Circular) (Six-base) MAPSORT of: pF1KB8625.seq Check: 3036 from: 1 to: 4106
pF1KB8625 4106 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1144 1144
Size: 4106
Acc65I G'GTAC_C
Cuts at: 1122 1122
Size: 4106
AclI AA'CG_TT
Cuts at: 1100 3946 1100
Size: 2846 1260
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 604 1001 1066 1931 2363 2716 3378 189
Size: 415 397 65 865 432 353 662 917
Fragments arranged by size:
917 865 662 432 415 397 353 65
AflIII A'CryG_T
Cuts at: 1458 3258 3539 1458
Size: 1800 281 2025
Fragments arranged by size:
2025 1800 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 118 150 118
Size: 32 4074
AlwNI CAG_nnn'CTG
Cuts at: 639 1411 2849 639
Size: 772 1438 1896
Fragments arranged by size:
1896 1438 772
ApaI G_GGCC'C
Cuts at: 413 413
Size: 4106
ApaLI G'TGCA_C
Cuts at: 2944 2944
Size: 4106
ApoI r'AATT_y
Cuts at: 924 1093 1110 3459 3715 924
Size: 169 17 2349 256 1315
Fragments arranged by size:
2349 1315 256 169 17
AseI AT'TA_AT
Cuts at: 20 20
Size: 4106
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
AvaI C'yCGr_G
Cuts at: 1126 1126
Size: 4106
AvrII C'CTAG_G
Cuts at: 594 594
Size: 4106
BamHI G'GATC_C
Cuts at: 1131 1131
Size: 4106
BanI G'GyrC_C
Cuts at: 722 1122 1536 1784 1819 722
Size: 400 414 248 35 3009
Fragments arranged by size:
3009 414 400 248 35
BanII G_rGCy'C
Cuts at: 333 413 740 773 802 1120 2150 333
Size: 80 327 33 29 318 1030 2289
Fragments arranged by size:
2289 1030 327 318 80 33 29
BbeI G_GCGC'C
Cuts at: 1540 1788 1540
Size: 248 3858
BbsI GAAGACnn'nnnn_
Cuts at: 316 316
Size: 4106
BciVI GTATCCnnnnn_n'
Cuts at: 531 2004 3060 4106 531
Size: 1473 1056 1046 531
Fragments arranged by size:
1473 1056 1046 531
BclI T'GATC_A
Cuts at: 1626 1626
Size: 4106
BglI GCCn_nnn'nGGC
Cuts at: 379 379
Size: 4106
BglII A'GATC_T
Cuts at: 1621 3658 1621
Size: 2037 2069
BlpI GC'TnA_GC
Cuts at: 161 1218 161
Size: 1057 3049
Bme1580I G_kGCm'C
Cuts at: 413 1731 1824 2948 413
Size: 1318 93 1124 1571
Fragments arranged by size:
1571 1318 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1318 1399 1734 3878 1318
Size: 81 335 2144 1546
Fragments arranged by size:
2144 1546 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 816 2473 816
Size: 1657 2449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 862 862
Size: 4106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 724 1282 2161 2875 3173 487
Size: 237 558 879 714 298 1420
Fragments arranged by size:
1420 879 714 558 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 394 3810 394
Size: 3416 690
BsaAI yAC'GTr
Cuts at: 1459 1459
Size: 4106
BsaBI GATnn'nnATC
Cuts at: 6 1064 1620 3657 6
Size: 1058 556 2037 455
Fragments arranged by size:
2037 1058 556 455
BsaHI Gr'CG_yC
Cuts at: 1537 1785 2487 3983 1537
Size: 248 702 1496 1660
Fragments arranged by size:
1660 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1068 1295 1816 2494 2905 3052 3326 1068
Size: 227 521 678 411 147 274 1848
Fragments arranged by size:
1848 678 521 411 274 227 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 118 148 787 817 118
Size: 30 639 30 3407
Fragments arranged by size:
3407 639 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 139 142 257 568 808 139
Size: 3 115 311 240 3437
Fragments arranged by size:
3437 311 240 115 3
BseYI C'CCAG_C
Cuts at: 497 1531 2289 2954 3362 497
Size: 1034 758 665 408 1241
Fragments arranged by size:
1241 1034 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 202
Size: 4106
BsiEI CG_ry'CG
Cuts at: 104 1648 1694 2924 104
Size: 1544 46 1230 1286
Fragments arranged by size:
1544 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 773 802 1120 1898 2088 2948 773
Size: 29 318 778 190 860 1931
Fragments arranged by size:
1931 860 778 318 190 29
Bsp1286I G_dGCh'C
Cuts at: 333 413 740 773 802 1120 1731 1824 1898
Size: 80 327 33 29 318 611 93 74
Cuts at: 1898 2088 2150 2948 333
Size: 190 62 798 1491
Fragments arranged by size:
1491 798 611 327 318 190 93 80
74 62 33 29
BspEI T'CCGG_A
Cuts at: 1068 1068
Size: 4106
BspHI T'CATG_A
Cuts at: 489 2538 489
Size: 2049 2057
BspMI ACCTGCnnnn'nnnn_
Cuts at: 620 1156 1370 1672 2053 620
Size: 536 214 302 381 2673
Fragments arranged by size:
2673 536 381 302 214
BsrBI CCG'CTC
Cuts at: 50 132 348 366 2398 2452 3936 4101 50
Size: 82 216 18 2032 54 1484 165 55
Fragments arranged by size:
2032 1484 216 165 82 55 54 18
BsrDI GCAATG_nn'
Cuts at: 2018 2018
Size: 4106
BsrFI r'CCGG_y
Cuts at: 2104 2285 2104
Size: 181 3925
BssSI C'ACGA_G
Cuts at: 169 801 2377 3085 169
Size: 632 1576 708 1190
Fragments arranged by size:
1576 1190 708 632
BstAPI GCAn_nnn'nTGC
Cuts at: 1483 1483
Size: 4106
BstBI TT'CG_AA
Cuts at: 2467 2467
Size: 4106
BstYI r'GATC_y
Cuts at: 1 1131 1598 1621 1956 2202 2508 2520 2606
Size: 1130 467 23 335 246 306 12 86
Cuts at: 2606 3658 1
Size: 1052 449
Fragments arranged by size:
1130 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 405 405
Size: 4106
BtgI C'CryG_G
Cuts at: 107 155 1001 107
Size: 48 846 3212
Fragments arranged by size:
3212 846 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2056 2237 2400 2056
Size: 181 163 3762
Fragments arranged by size:
3762 181 163
BtsI GCAGTG_nn'
Cuts at: 1349 1349
Size: 4106
DraI TTT'AAA
Cuts at: 1105 1105
Size: 4106
DrdI GACnn_nn'nnGTC
Cuts at: 658 1812 3156 658
Size: 1154 1344 1608
Fragments arranged by size:
1608 1344 1154
EaeI y'GGCC_r
Cuts at: 152 371 380 1691 1865 2256 2283 3628 152
Size: 219 9 1311 174 391 27 1345 630
Fragments arranged by size:
1345 1311 630 391 219 174 27 9
EagI C'GGCC_G
Cuts at: 1691 1691
Size: 4106
EarI CTCTTCn'nnn_
Cuts at: 319 431 2129 2339 319
Size: 112 1698 210 2086
Fragments arranged by size:
2086 1698 210 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 128 376 2314 3056 3202 3916 128
Size: 248 1938 742 146 714 318
Fragments arranged by size:
1938 742 714 318 248 146
EcoICRI GAG'CTC
Cuts at: 771 800 1118 771
Size: 29 318 3759
Fragments arranged by size:
3759 318 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 604 862 1001 1066 1931 2363 2716 3378
Size: 415 258 139 65 865 432 353 662
Cuts at: 3378 189
Size: 917
Fragments arranged by size:
917 865 662 432 415 353 258 139
65
EcoO109I rG'GnC_Cy
Cuts at: 409 410 1245 409
Size: 1 835 3270
Fragments arranged by size:
3270 835 1
EcoRI G'AATT_C
Cuts at: 924 1110 924
Size: 186 3920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2125 2125
Size: 4106
FspI TGC'GCA
Cuts at: 1887 1887
Size: 4106
HaeII r_GCGC'y
Cuts at: 1540 1788 3018 3789 1540
Size: 248 1230 771 1857
Fragments arranged by size:
1857 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 822 854 1948 1978 1980 2010 822
Size: 32 1094 30 2 30 2918
Fragments arranged by size:
2918 1094 32 30 30 2
HincII GTy'rAC
Cuts at: 1145 1145
Size: 4106
HindIII A'AGCT_T
Cuts at: 265 265
Size: 4106
KasI G'GCGC_C
Cuts at: 1536 1784 1536
Size: 248 3858
KpnI G_GTAC'C
Cuts at: 1126 1126
Size: 4106
MluI A'CGCG_T
Cuts at: 3539 3539
Size: 4106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 623 2865 3049 623
Size: 2242 184 1680
Fragments arranged by size:
2242 1680 184
MscI TGG'CCA
Cuts at: 154 382 1867 154
Size: 228 1485 2393
Fragments arranged by size:
2393 1485 228
MslI CAynn'nnrTG
Cuts at: 703 2095 2222 703
Size: 1392 127 2587
Fragments arranged by size:
2587 1392 127
MspA1I CmG'CkG
Cuts at: 300 626 717 1217 1531 1891 2675 2920 300
Size: 326 91 500 314 360 784 245 1486
Fragments arranged by size:
1486 784 500 360 326 314 245 91
NaeI GCC'GGC
Cuts at: 2287 2287
Size: 4106
NarI GG'CG_CC
Cuts at: 1537 1785 1537
Size: 248 3858
NcoI C'CATG_G
Cuts at: 107 155 1001 107
Size: 48 846 3212
Fragments arranged by size:
3212 846 48
NgoMIV G'CCGG_C
Cuts at: 2285 2285
Size: 4106
NspI r_CATG'y
Cuts at: 147 1159 1659 3262 147
Size: 1012 500 1603 991
Fragments arranged by size:
1603 1012 991 500
PasI CC'CwG_GG
Cuts at: 526 526
Size: 4106
PciI A'CATG_T
Cuts at: 3258 3258
Size: 4106
PmeI GTTT'AAAC
Cuts at: 1105 1105
Size: 4106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 118 150 2552 2584 118
Size: 32 2402 32 1640
Fragments arranged by size:
2402 1640 32 32
PshAI GACnn'nnGTC
Cuts at: 996 996
Size: 4106
PspOMI G'GGCC_C
Cuts at: 409 409
Size: 4106
PstI C_TGCA'G
Cuts at: 455 1153 1838 455
Size: 698 685 2723
Fragments arranged by size:
2723 698 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4106
PvuII CAG'CTG
Cuts at: 300 1531 1891 300
Size: 1231 360 2515
Fragments arranged by size:
2515 1231 360
RsrII CG'GwC_CG
Cuts at: 2301 2301
Size: 4106
SacI G_AGCT'C
Cuts at: 773 802 1120 773
Size: 29 318 3759
Fragments arranged by size:
3759 318 29
SalI G'TCGA_C
Cuts at: 1143 1143
Size: 4106
SapI GCTCTTCn'nnn_
Cuts at: 2129 2339 2129
Size: 210 3896
SbfI CC_TGCA'GG
Cuts at: 1153 1153
Size: 4106
ScaI AGT'ACT
Cuts at: 3623 3623
Size: 4106
SfcI C'TryA_G
Cuts at: 33 451 1149 1834 2802 2993 33
Size: 418 698 685 968 191 1146
Fragments arranged by size:
1146 968 698 685 418 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 379 379
Size: 4106
SfoI GGC'GCC
Cuts at: 1538 1786 1538
Size: 248 3858
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
SmaI CCC'GGG
Cuts at: 1128 1128
Size: 4106
SmlI C'TyrA_G
Cuts at: 502 739 1261 2176 2890 3152 502
Size: 237 522 915 714 262 1456
Fragments arranged by size:
1456 915 714 522 262 237
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4106
SphI G_CATG'C
Cuts at: 1159 1659 1159
Size: 500 3606
StyI C'CwwG_G
Cuts at: 107 155 383 594 1001 1085 1240 1594 107
Size: 48 228 211 407 84 155 354 2619
Fragments arranged by size:
2619 407 354 228 211 155 84 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1689 1822 2281 2490 1689
Size: 133 459 209 3305
Fragments arranged by size:
3305 459 209 133
TatI w'GTAC_w
Cuts at: 3621 3621
Size: 4106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 787 819 3623 3655 787
Size: 32 2804 32 1238
Fragments arranged by size:
2804 1238 32 32
Tth111I GACn'n_nGTC
Cuts at: 639 1903 639
Size: 1264 2842
XbaI T'CTAG_A
Cuts at: 1137 1137
Size: 4106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1601 1601
Size: 4106
XmaI C'CCGG_G
Cuts at: 1126 1126
Size: 4106
XmnI GAAnn'nnTTC
Cuts at: 199 1051 199
Size: 852 3254
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PmeI PpiI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AscI BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PpuMI PsiI PspXI PsrI SacII SanDI
SexAI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI