Restriction map for
pF1KB8619
(Circular) (Six-base) MAPSORT of: pF1KB8619.seq Check: 1466 from: 1 to: 4061
pF1KB8619 4061 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1099 1099
Size: 4061
Acc65I G'GTAC_C
Cuts at: 1077 1077
Size: 4061
AclI AA'CG_TT
Cuts at: 3901 3901
Size: 4061
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 330 1886 2318 2671 3333 330
Size: 1556 432 353 662 1058
Fragments arranged by size:
1556 1058 662 432 353
AflIII A'CryG_T
Cuts at: 1413 3213 3494 1413
Size: 1800 281 1980
Fragments arranged by size:
1980 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 390 960 1044 1366 2804 390
Size: 570 84 322 1438 1647
Fragments arranged by size:
1647 1438 570 322 84
ApaI G_GGCC'C
Cuts at: 695 695
Size: 4061
ApaLI G'TGCA_C
Cuts at: 2899 2899
Size: 4061
ApoI r'AATT_y
Cuts at: 139 1065 3414 3670 139
Size: 926 2349 256 530
Fragments arranged by size:
2349 926 530 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4061
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
AvaI C'yCGr_G
Cuts at: 1081 1081
Size: 4061
BamHI G'GATC_C
Cuts at: 1086 1086
Size: 4061
BanI G'GyrC_C
Cuts at: 443 633 1077 1491 1739 1774 443
Size: 190 444 414 248 35 2730
Fragments arranged by size:
2730 444 414 248 190 35
BanII G_rGCy'C
Cuts at: 244 695 1075 2105 244
Size: 451 380 1030 2200
Fragments arranged by size:
2200 1030 451 380
BbeI G_GCGC'C
Cuts at: 1495 1743 1495
Size: 248 3813
BbsI GAAGACnn'nnnn_
Cuts at: 735 735
Size: 4061
BciVI GTATCCnnnnn_n'
Cuts at: 1959 3015 4061 1959
Size: 1056 1046 1959
Fragments arranged by size:
1959 1056 1046
BclI T'GATC_A
Cuts at: 1581 1581
Size: 4061
BglI GCCn_nnn'nGGC
Cuts at: 499 925 499
Size: 426 3635
BglII A'GATC_T
Cuts at: 1576 3613 1576
Size: 2037 2024
BlpI GC'TnA_GC
Cuts at: 1173 1173
Size: 4061
Bme1580I G_kGCm'C
Cuts at: 224 638 695 1686 1779 2903 224
Size: 414 57 991 93 1124 1382
Fragments arranged by size:
1382 1124 991 414 93 57
BmgBI CAC'GTC
Cuts at: 372 372
Size: 4061
BmrI ACTGGGnnnn_n'
Cuts at: 573 1273 1354 1689 3833 573
Size: 700 81 335 2144 801
Fragments arranged by size:
2144 801 700 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 727
Size: 4061
Bpu10I CC'TnA_GC
Cuts at: 243 243
Size: 4061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1237 2116 2830 3128 1237
Size: 879 714 298 2170
Fragments arranged by size:
2170 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3765 3765
Size: 4061
BsaAI yAC'GTr
Cuts at: 225 1414 225
Size: 1189 2872
BsaBI GATnn'nnATC
Cuts at: 6 1575 3612 6
Size: 1569 2037 455
Fragments arranged by size:
2037 1569 455
BsaHI Gr'CG_yC
Cuts at: 1492 1740 2442 3938 1492
Size: 248 702 1496 1615
Fragments arranged by size:
1615 1496 702 248
BsaWI w'CCGG_w
Cuts at: 395 966 1250 1771 2449 2860 3007 3281 395
Size: 571 284 521 678 411 147 274 1175
Fragments arranged by size:
1175 678 571 521 411 284 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 700 715 730 745 700
Size: 15 15 15 4016
Fragments arranged by size:
4016 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 292 295 564 971 974 160
Size: 132 3 269 407 3 3247
Fragments arranged by size:
3247 407 269 132 3 3
BseYI C'CCAG_C
Cuts at: 1486 2244 2909 3317 1486
Size: 758 665 408 2230
Fragments arranged by size:
2230 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 119 463 1603 1649 2879 104
Size: 15 344 1140 46 1230 1286
Fragments arranged by size:
1286 1230 1140 344 46 15
BsiHKAI G_wGCw'C
Cuts at: 1075 1853 2043 2903 1075
Size: 778 190 860 2233
Fragments arranged by size:
2233 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 224 244 638 695 1075 1686 1779 1853 2043
Size: 20 394 57 380 611 93 74 190
Cuts at: 2043 2105 2903 224
Size: 62 798 1382
Fragments arranged by size:
1382 798 611 394 380 190 93 74
62 57 20
BspEI T'CCGG_A
Cuts at: 966 966
Size: 4061
BspHI T'CATG_A
Cuts at: 2493 2493
Size: 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1111 1325 1627 2008 1111
Size: 214 302 381 3164
Fragments arranged by size:
3164 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2353 2407 3891 4056 50
Size: 2303 54 1484 165 55
Fragments arranged by size:
2303 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1973 1973
Size: 4061
BsrFI r'CCGG_y
Cuts at: 2059 2240 2059
Size: 181 3880
BssSI C'ACGA_G
Cuts at: 2332 3040 2332
Size: 708 3353
BstAPI GCAn_nnn'nTGC
Cuts at: 450 617 1438 450
Size: 167 821 3073
Fragments arranged by size:
3073 821 167
BstBI TT'CG_AA
Cuts at: 2422 2422
Size: 4061
BstEII G'GTnAC_C
Cuts at: 591 591
Size: 4061
BstXI CCAn_nnnn'nTGG
Cuts at: 879 879
Size: 4061
BstYI r'GATC_y
Cuts at: 1 465 485 1086 1553 1576 1911 2157 2463
Size: 464 20 601 467 23 335 246 306
Cuts at: 2463 2475 2561 3613 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 601 467 464 449 335 306 246
86 23 20 12
BtgI C'CryG_G
Cuts at: 107 236 596 752 854 899 929 107
Size: 129 360 156 102 45 30 3239
Fragments arranged by size:
3239 360 156 129 102 45 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 999 2011 2192 2355 999
Size: 1012 181 163 2705
Fragments arranged by size:
2705 1012 181 163
BtsI GCAGTG_nn'
Cuts at: 409 796 1304 409
Size: 387 508 3166
Fragments arranged by size:
3166 508 387
DraI TTT'AAA
Cuts at: 1060 1060
Size: 4061
DraIII CAC_nnn'GTG
Cuts at: 591 591
Size: 4061
DrdI GACnn_nn'nnGTC
Cuts at: 1767 3111 1767
Size: 1344 2717
EaeI y'GGCC_r
Cuts at: 116 233 426 460 512 881 926 1646 1820
Size: 117 193 34 52 369 45 720 174
Cuts at: 1820 2211 2238 3583 116
Size: 391 27 1345 594
Fragments arranged by size:
1345 720 594 391 369 193 174 117
52 45 34 27
EagI C'GGCC_G
Cuts at: 116 460 1646 116
Size: 344 1186 2531
Fragments arranged by size:
2531 1186 344
EarI CTCTTCn'nnn_
Cuts at: 2084 2294 2084
Size: 210 3851
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2269 3011 3157 3871 2269
Size: 742 146 714 2459
Fragments arranged by size:
2459 742 714 146
EcoICRI GAG'CTC
Cuts at: 1073 1073
Size: 4061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 330 727 1886 2318 2671 3333 330
Size: 397 1159 432 353 662 1058
Fragments arranged by size:
1159 1058 662 432 397 353
EcoO109I rG'GnC_Cy
Cuts at: 387 555 691 692 1200 387
Size: 168 136 1 508 3248
Fragments arranged by size:
3248 508 168 136 1
EcoRI G'AATT_C
Cuts at: 139 1065 139
Size: 926 3135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 963 2080 963
Size: 1117 2944
FspI TGC'GCA
Cuts at: 1842 1842
Size: 4061
HaeII r_GCGC'y
Cuts at: 808 1495 1743 2973 3744 808
Size: 687 248 1230 771 1125
Fragments arranged by size:
1230 1125 771 687 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1903 1933 1935 1965 1903
Size: 30 2 30 3999
Fragments arranged by size:
3999 30 30 2
HincII GTy'rAC
Cuts at: 1100 1100
Size: 4061
KasI G'GCGC_C
Cuts at: 1491 1739 1491
Size: 248 3813
KpnI G_GTAC'C
Cuts at: 1081 1081
Size: 4061
MluI A'CGCG_T
Cuts at: 3494 3494
Size: 4061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2820 3004 2820
Size: 184 3877
MscI TGG'CCA
Cuts at: 428 883 928 1822 428
Size: 455 45 894 2667
Fragments arranged by size:
2667 894 455 45
MslI CAynn'nnrTG
Cuts at: 751 775 2050 2177 751
Size: 24 1275 127 2635
Fragments arranged by size:
2635 1275 127 24
MspA1I CmG'CkG
Cuts at: 207 1172 1486 1846 2630 2875 207
Size: 965 314 360 784 245 1393
Fragments arranged by size:
1393 965 784 360 314 245
NaeI GCC'GGC
Cuts at: 2242 2242
Size: 4061
NarI GG'CG_CC
Cuts at: 1492 1740 1492
Size: 248 3813
NcoI C'CATG_G
Cuts at: 107 752 929 107
Size: 645 177 3239
Fragments arranged by size:
3239 645 177
NgoMIV G'CCGG_C
Cuts at: 2240 2240
Size: 4061
NspI r_CATG'y
Cuts at: 1114 1614 3217 1114
Size: 500 1603 1958
Fragments arranged by size:
1958 1603 500
PasI CC'CwG_GG
Cuts at: 813 813
Size: 4061
PciI A'CATG_T
Cuts at: 3213 3213
Size: 4061
PfoI T'CCnGG_A
Cuts at: 389 677 704 389
Size: 288 27 3746
Fragments arranged by size:
3746 288 27
PmeI GTTT'AAAC
Cuts at: 1060 1060
Size: 4061
PmlI CAC'GTG
Cuts at: 225 225
Size: 4061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2507 2539 2507
Size: 32 4029
PpuMI rG'GwC_Cy
Cuts at: 387 555 387
Size: 168 3893
PspOMI G'GGCC_C
Cuts at: 691 691
Size: 4061
PstI C_TGCA'G
Cuts at: 308 791 1108 1793 308
Size: 483 317 685 2576
Fragments arranged by size:
2576 685 483 317
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4061
PvuII CAG'CTG
Cuts at: 207 1486 1846 207
Size: 1279 360 2422
Fragments arranged by size:
2422 1279 360
RsrII CG'GwC_CG
Cuts at: 2256 2256
Size: 4061
SacI G_AGCT'C
Cuts at: 1075 1075
Size: 4061
SalI G'TCGA_C
Cuts at: 1098 1098
Size: 4061
SanDI GG'GwC_CC
Cuts at: 555 555
Size: 4061
SapI GCTCTTCn'nnn_
Cuts at: 2084 2294 2084
Size: 210 3851
SbfI CC_TGCA'GG
Cuts at: 308 1108 308
Size: 800 3261
ScaI AGT'ACT
Cuts at: 3578 3578
Size: 4061
SfcI C'TryA_G
Cuts at: 33 304 570 787 1104 1789 2757 2948 33
Size: 271 266 217 317 685 968 191 1146
Fragments arranged by size:
1146 968 685 317 271 266 217 191
SfoI GGC'GCC
Cuts at: 1493 1741 1493
Size: 248 3813
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
SmaI CCC'GGG
Cuts at: 1083 1083
Size: 4061
SmlI C'TyrA_G
Cuts at: 1216 2131 2845 3107 1216
Size: 915 714 262 2170
Fragments arranged by size:
2170 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4061
SphI G_CATG'C
Cuts at: 1114 1614 1114
Size: 500 3561
StyI C'CwwG_G
Cuts at: 107 752 781 929 1195 1549 107
Size: 645 29 148 266 354 2619
Fragments arranged by size:
2619 645 354 266 148 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1644 1777 2236 2445 1644
Size: 133 459 209 3260
Fragments arranged by size:
3260 459 209 133
TatI w'GTAC_w
Cuts at: 944 3576 944
Size: 2632 1429
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 700 732 3578 3610 700
Size: 32 2846 32 1151
Fragments arranged by size:
2846 1151 32 32
Tth111I GACn'n_nGTC
Cuts at: 1858 1858
Size: 4061
XbaI T'CTAG_A
Cuts at: 1092 1092
Size: 4061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 159 1556 159
Size: 1397 2664
XmaI C'CCGG_G
Cuts at: 1081 1081
Size: 4061
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI