Restriction map for
pF1KB8616
(Circular) (Six-base) MAPSORT of: pF1KB8616.seq Check: 593 from: 1 to: 4217
pF1KB8616 4217 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AatII G_ACGT'C
Cuts at: 510 510
Size: 4217
AccI GT'mk_AC
Cuts at: 1255 1255
Size: 4217
Acc65I G'GTAC_C
Cuts at: 591 1233 591
Size: 642 3575
AclI AA'CG_TT
Cuts at: 4057 4057
Size: 4217
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 626 1027 1139 2042 2474 3221 3489 521
Size: 105 401 112 903 432 747 268 1249
Fragments arranged by size:
1249 903 747 432 401 268 112 105
AflIII A'CryG_T
Cuts at: 1569 2673 3650 1569
Size: 1104 977 2136
Fragments arranged by size:
2136 1104 977
AgeI A'CCGG_T
Cuts at: 2605 2605
Size: 4217
AhdI GACnn_n'nnGTC
Cuts at: 1194 1194
Size: 4217
AlwNI CAG_nnn'CTG
Cuts at: 1522 3089 1522
Size: 1567 2650
ApaI G_GGCC'C
Cuts at: 568 568
Size: 4217
ApaLI G'TGCA_C
Cuts at: 2987 2987
Size: 4217
ApoI r'AATT_y
Cuts at: 223 408 850 1207 1221 3570 3826 223
Size: 185 442 357 14 2349 256 614
Fragments arranged by size:
2349 614 442 357 256 185 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 4217
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4217
AvaI C'yCGr_G
Cuts at: 145 1237 145
Size: 1092 3125
BamHI G'GATC_C
Cuts at: 1242 1242
Size: 4217
BanI G'GyrC_C
Cuts at: 591 1233 1647 1895 1930 591
Size: 642 414 248 35 2878
Fragments arranged by size:
2878 642 414 248 35
BanII G_rGCy'C
Cuts at: 137 568 1231 2261 137
Size: 431 663 1030 2093
Fragments arranged by size:
2093 1030 663 431
BbeI G_GCGC'C
Cuts at: 1651 1899 1651
Size: 248 3969
BbsI GAAGACnn'nnnn_
Cuts at: 1145 1145
Size: 4217
BciVI GTATCCnnnnn_n'
Cuts at: 2115 2876 4217 2115
Size: 761 1341 2115
Fragments arranged by size:
2115 1341 761
BclI T'GATC_A
Cuts at: 1737 1737
Size: 4217
BglII A'GATC_T
Cuts at: 257 1732 3769 257
Size: 1475 2037 705
Fragments arranged by size:
2037 1475 705
BlpI GC'TnA_GC
Cuts at: 1329 1329
Size: 4217
Bme1580I G_kGCm'C
Cuts at: 568 839 1842 1935 2991 568
Size: 271 1003 93 1056 1794
Fragments arranged by size:
1794 1056 1003 271 93
BmrI ACTGGGnnnn_n'
Cuts at: 826 1429 1510 1845 3989 826
Size: 603 81 335 2144 1054
Fragments arranged by size:
2144 1054 603 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 932 2584 932
Size: 1652 2565
Bpu10I CC'TnA_GC
Cuts at: 358 358
Size: 4217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1393 2272 2764 3062 1393
Size: 879 492 298 2548
Fragments arranged by size:
2548 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 920 3921 920
Size: 3001 1216
BsaAI yAC'GTr
Cuts at: 1570 1570
Size: 4217
BsaBI GATnn'nnATC
Cuts at: 6 1731 3768 6
Size: 1725 2037 455
Fragments arranged by size:
2037 1725 455
BsaHI Gr'CG_yC
Cuts at: 507 1648 1896 2598 4094 507
Size: 1141 248 702 1496 630
Fragments arranged by size:
1496 1141 702 630 248
BsaWI w'CCGG_w
Cuts at: 1406 1927 2605 2879 3026 3437 1406
Size: 521 678 274 147 411 2186
Fragments arranged by size:
2186 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1084 1084
Size: 4217
BseYI C'CCAG_C
Cuts at: 567 839 1642 2400 2977 3473 567
Size: 272 803 758 577 496 1311
Fragments arranged by size:
1311 803 758 577 496 272
BsiEI CG_ry'CG
Cuts at: 104 1759 1805 3013 104
Size: 1655 46 1208 1308
Fragments arranged by size:
1655 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 115 1231 2009 2199 2991 115
Size: 1116 778 190 792 1341
Fragments arranged by size:
1341 1116 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 115 137 568 839 1231 1842 1935 2009 2199
Size: 22 431 271 392 611 93 74 190
Cuts at: 2199 2261 2991 115
Size: 62 730 1341
Fragments arranged by size:
1341 730 611 431 392 271 190 93
74 62 22
BspEI T'CCGG_A
Cuts at: 3437 3437
Size: 4217
BspHI T'CATG_A
Cuts at: 3393 3393
Size: 4217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1267 1481 1783 2164 1267
Size: 214 302 381 3320
Fragments arranged by size:
3320 381 302 214
BsrBI CCG'CTC
Cuts at: 50 936 2509 2563 4047 4212 50
Size: 886 1573 54 1484 165 55
Fragments arranged by size:
1573 1484 886 165 55 54
BsrDI GCAATG_nn'
Cuts at: 290 707 897 2129 290
Size: 417 190 1232 2378
Fragments arranged by size:
2378 1232 417 190
BsrFI r'CCGG_y
Cuts at: 2215 2396 2605 2215
Size: 181 209 3827
Fragments arranged by size:
3827 209 181
BssSI C'ACGA_G
Cuts at: 2488 2846 2488
Size: 358 3859
BstAPI GCAn_nnn'nTGC
Cuts at: 1594 1594
Size: 4217
BstBI TT'CG_AA
Cuts at: 2578 2578
Size: 4217
BstXI CCAn_nnnn'nTGG
Cuts at: 502 910 502
Size: 408 3809
BstYI r'GATC_y
Cuts at: 1 257 1242 1709 1732 2067 2313 3325 3411
Size: 256 985 467 23 335 246 1012 86
Cuts at: 3411 3423 3769 1
Size: 12 346 449
Fragments arranged by size:
1012 985 467 449 346 335 256 246
86 23 12
BtgI C'CryG_G
Cuts at: 123 625 123
Size: 502 3715
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2167 2348 2511 2167
Size: 181 163 3873
Fragments arranged by size:
3873 181 163
BtsI GCAGTG_nn'
Cuts at: 1460 1460
Size: 4217
DraI TTT'AAA
Cuts at: 855 1216 855
Size: 361 3856
DrdI GACnn_nn'nnGTC
Cuts at: 506 1923 2781 506
Size: 1417 858 1942
Fragments arranged by size:
1942 1417 858
EaeI y'GGCC_r
Cuts at: 1802 1976 2367 2394 3739 1802
Size: 174 391 27 1345 2280
Fragments arranged by size:
2280 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1802 1802
Size: 4217
EarI CTCTTCn'nnn_
Cuts at: 524 901 926 2240 2450 524
Size: 377 25 1314 210 2291
Fragments arranged by size:
2291 1314 377 210 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2425 2735 2881 4027 2425
Size: 310 146 1146 2615
Fragments arranged by size:
2615 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1229 1229
Size: 4217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 626 1027 1139 2042 2474 3221 3489 521
Size: 105 401 112 903 432 747 268 1249
Fragments arranged by size:
1249 903 747 432 401 268 112 105
EcoO109I rG'GnC_Cy
Cuts at: 564 1356 564
Size: 792 3425
EcoRI G'AATT_C
Cuts at: 408 1207 1221 408
Size: 799 14 3404
Fragments arranged by size:
3404 799 14
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 4217
FspI TGC'GCA
Cuts at: 214 1998 214
Size: 1784 2433
HaeII r_GCGC'y
Cuts at: 198 1651 1899 2921 3900 198
Size: 1453 248 1022 979 515
Fragments arranged by size:
1453 1022 979 515 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 418 450 1122 1154 1180 1183 1212 1215 2059
Size: 32 672 32 26 3 29 3 844
Cuts at: 2059 2089 2091 2121 418
Size: 30 2 30 2514
Fragments arranged by size:
2514 844 672 32 32 30 30 29
26 3 3 2
HincII GTy'rAC
Cuts at: 1256 1256
Size: 4217
KasI G'GCGC_C
Cuts at: 1647 1895 1647
Size: 248 3969
KpnI G_GTAC'C
Cuts at: 595 1237 595
Size: 642 3575
MfeI C'AATT_G
Cuts at: 371 371
Size: 4217
MluI A'CGCG_T
Cuts at: 3650 3650
Size: 4217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2888 3072 2888
Size: 184 4033
MscI TGG'CCA
Cuts at: 1978 1978
Size: 4217
MslI CAynn'nnrTG
Cuts at: 805 962 2206 2333 805
Size: 157 1244 127 2689
Fragments arranged by size:
2689 1244 157 127
MspA1I CmG'CkG
Cuts at: 125 162 627 843 1014 1328 1642 2002 3015
Size: 37 465 216 171 314 314 360 1013
Cuts at: 3015 3260 125
Size: 245 1082
Fragments arranged by size:
1082 1013 465 360 314 314 245 216
171 37
NaeI GCC'GGC
Cuts at: 2398 2398
Size: 4217
NarI GG'CG_CC
Cuts at: 1648 1896 1648
Size: 248 3969
NdeI CA'TA_TG
Cuts at: 1001 1001
Size: 4217
NgoMIV G'CCGG_C
Cuts at: 2396 2396
Size: 4217
NspI r_CATG'y
Cuts at: 1270 1770 2677 1270
Size: 500 907 2810
Fragments arranged by size:
2810 907 500
PciI A'CATG_T
Cuts at: 2673 2673
Size: 4217
PfoI T'CCnGG_A
Cuts at: 444 444
Size: 4217
PmeI GTTT'AAAC
Cuts at: 1216 1216
Size: 4217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3356 3388 3356
Size: 32 4185
PsiI TTA'TAA
Cuts at: 1136 1136
Size: 4217
PspOMI G'GGCC_C
Cuts at: 564 564
Size: 4217
PstI C_TGCA'G
Cuts at: 1264 1949 1264
Size: 685 3532
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4217
PvuII CAG'CTG
Cuts at: 843 1014 1642 2002 843
Size: 171 628 360 3058
Fragments arranged by size:
3058 628 360 171
RsrII CG'GwC_CG
Cuts at: 2412 2412
Size: 4217
SacI G_AGCT'C
Cuts at: 1231 1231
Size: 4217
SacII CC_GC'GG
Cuts at: 126 628 126
Size: 502 3715
SalI G'TCGA_C
Cuts at: 1254 1254
Size: 4217
SapI GCTCTTCn'nnn_
Cuts at: 926 2240 2450 926
Size: 1314 210 2693
Fragments arranged by size:
2693 1314 210
SbfI CC_TGCA'GG
Cuts at: 1264 1264
Size: 4217
ScaI AGT'ACT
Cuts at: 750 3734 750
Size: 2984 1233
SfcI C'TryA_G
Cuts at: 33 458 1260 1945 2938 3129 33
Size: 425 802 685 993 191 1121
Fragments arranged by size:
1121 993 802 685 425 191
SfoI GGC'GCC
Cuts at: 1649 1897 1649
Size: 248 3969
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4217
SmaI CCC'GGG
Cuts at: 1239 1239
Size: 4217
SmlI C'TyrA_G
Cuts at: 1372 2287 2779 3041 1372
Size: 915 492 262 2548
Fragments arranged by size:
2548 915 492 262
SpeI A'CTAG_T
Cuts at: 67 438 67
Size: 371 3846
SphI G_CATG'C
Cuts at: 1270 1770 1270
Size: 500 3717
SspI AAT'ATT
Cuts at: 1119 1119
Size: 4217
StuI AGG'CCT
Cuts at: 767 767
Size: 4217
StyI C'CwwG_G
Cuts at: 717 1034 1351 1705 717
Size: 317 317 354 3229
Fragments arranged by size:
3229 354 317 317
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1800 1933 2392 2601 1800
Size: 133 459 209 3416
Fragments arranged by size:
3416 459 209 133
TatI w'GTAC_w
Cuts at: 548 748 3732 548
Size: 200 2984 1033
Fragments arranged by size:
2984 1033 200
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3734 3766 3734
Size: 32 4185
Tth111I GACn'n_nGTC
Cuts at: 2014 2014
Size: 4217
XbaI T'CTAG_A
Cuts at: 1248 1248
Size: 4217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1712 1712
Size: 4217
XmaI C'CCGG_G
Cuts at: 1237 1237
Size: 4217
ZraI GAC'GTC
Cuts at: 508 508
Size: 4217
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PciI PfoI PmeI PpiI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI NcoI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
XmnI