Restriction map for
pF1KB8599
(Circular) (Six-base) MAPSORT of: pF1KB8599.seq Check: 7778 from: 1 to: 3845
pF1KB8599 3845 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 883 883
Size: 3845
Acc65I G'GTAC_C
Cuts at: 861 861
Size: 3845
AclI AA'CG_TT
Cuts at: 3685 3685
Size: 3845
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 492 1670 2102 2455 3117 292
Size: 200 1178 432 353 662 1020
Fragments arranged by size:
1178 1020 662 432 353 200
AflIII A'CryG_T
Cuts at: 1197 2997 3278 1197
Size: 1800 281 1764
Fragments arranged by size:
1800 1764 281
AlwNI CAG_nnn'CTG
Cuts at: 1150 2588 1150
Size: 1438 2407
ApaLI G'TGCA_C
Cuts at: 2683 2683
Size: 3845
ApoI r'AATT_y
Cuts at: 849 3198 3454 849
Size: 2349 256 1240
Fragments arranged by size:
2349 1240 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3845
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3845
AvaI C'yCGr_G
Cuts at: 123 865 123
Size: 742 3103
BamHI G'GATC_C
Cuts at: 870 870
Size: 3845
BanI G'GyrC_C
Cuts at: 328 861 1275 1523 1558 328
Size: 533 414 248 35 2615
Fragments arranged by size:
2615 533 414 248 35
BanII G_rGCy'C
Cuts at: 859 1889 859
Size: 1030 2815
BbeI G_GCGC'C
Cuts at: 1279 1527 1279
Size: 248 3597
BciVI GTATCCnnnnn_n'
Cuts at: 1743 2799 3845 1743
Size: 1056 1046 1743
Fragments arranged by size:
1743 1056 1046
BclI T'GATC_A
Cuts at: 1365 1365
Size: 3845
BglII A'GATC_T
Cuts at: 1360 3397 1360
Size: 2037 1808
BlpI GC'TnA_GC
Cuts at: 957 957
Size: 3845
Bme1580I G_kGCm'C
Cuts at: 1470 1563 2687 1470
Size: 93 1124 2628
Fragments arranged by size:
2628 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 294 1057 1138 1473 3617 294
Size: 763 81 335 2144 522
Fragments arranged by size:
2144 763 522 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 3845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 459 629 638 221
Size: 238 170 9 3428
Fragments arranged by size:
3428 238 170 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1021 1900 2614 2912 1021
Size: 879 714 298 1954
Fragments arranged by size:
1954 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3549 3549
Size: 3845
BsaAI yAC'GTr
Cuts at: 157 1198 157
Size: 1041 2804
BsaBI GATnn'nnATC
Cuts at: 6 1359 3396 6
Size: 1353 2037 455
Fragments arranged by size:
2037 1353 455
BsaHI Gr'CG_yC
Cuts at: 1276 1524 2226 3722 1276
Size: 248 702 1496 1399
Fragments arranged by size:
1496 1399 702 248
BsaWI w'CCGG_w
Cuts at: 1034 1555 2233 2644 2791 3065 1034
Size: 521 678 411 147 274 1814
Fragments arranged by size:
1814 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 419 449 627 657 419
Size: 30 178 30 3607
Fragments arranged by size:
3607 178 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 583 583
Size: 3845
BseYI C'CCAG_C
Cuts at: 1270 2028 2693 3101 1270
Size: 758 665 408 2014
Fragments arranged by size:
2014 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1387 1433 2663 104
Size: 1283 46 1230 1286
Fragments arranged by size:
1286 1283 1230 46
BsiHKAI G_wGCw'C
Cuts at: 859 1637 1827 2687 859
Size: 778 190 860 2017
Fragments arranged by size:
2017 860 778 190
BsmI GAATG_Cn'
Cuts at: 769 769
Size: 3845
Bsp1286I G_dGCh'C
Cuts at: 859 1470 1563 1637 1827 1889 2687 859
Size: 611 93 74 190 62 798 2017
Fragments arranged by size:
2017 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2277 2277
Size: 3845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 895 1109 1411 1792 895
Size: 214 302 381 2948
Fragments arranged by size:
2948 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2137 2191 3675 3840 50
Size: 2087 54 1484 165 55
Fragments arranged by size:
2087 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1757 1757
Size: 3845
BsrFI r'CCGG_y
Cuts at: 1843 2024 1843
Size: 181 3664
BsrGI T'GTAC_A
Cuts at: 758 758
Size: 3845
BssSI C'ACGA_G
Cuts at: 404 2116 2824 404
Size: 1712 708 1425
Fragments arranged by size:
1712 1425 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1222 1222
Size: 3845
BstBI TT'CG_AA
Cuts at: 2206 2206
Size: 3845
BstYI r'GATC_y
Cuts at: 1 870 1337 1360 1695 1941 2247 2259 2345
Size: 869 467 23 335 246 306 12 86
Cuts at: 2345 3397 1
Size: 1052 449
Fragments arranged by size:
1052 869 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 292 107
Size: 185 3660
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 256 487 1795 1976 2139 256
Size: 231 1308 181 163 1962
Fragments arranged by size:
1962 1308 231 181 163
BtsI GCAGTG_nn'
Cuts at: 824 1088 824
Size: 264 3581
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 186 221 818 853 186
Size: 35 597 35 3178
Fragments arranged by size:
3178 597 35 35
DraI TTT'AAA
Cuts at: 844 844
Size: 3845
DrdI GACnn_nn'nnGTC
Cuts at: 1551 2895 1551
Size: 1344 2501
EaeI y'GGCC_r
Cuts at: 110 1430 1604 1995 2022 3367 110
Size: 1320 174 391 27 1345 588
Fragments arranged by size:
1345 1320 588 391 174 27
EagI C'GGCC_G
Cuts at: 1430 1430
Size: 3845
EarI CTCTTCn'nnn_
Cuts at: 789 1868 2078 789
Size: 1079 210 2556
Fragments arranged by size:
2556 1079 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2053 2795 2941 3655 2053
Size: 742 146 714 2243
Fragments arranged by size:
2243 742 714 146
EcoICRI GAG'CTC
Cuts at: 857 857
Size: 3845
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 292 459 492 629 638 1670 2102 2455
Size: 71 167 33 137 9 1032 432 353
Cuts at: 2455 3117 221
Size: 662 949
Fragments arranged by size:
1032 949 662 432 353 167 137 71
33 9
EcoO109I rG'GnC_Cy
Cuts at: 702 984 702
Size: 282 3563
EcoRI G'AATT_C
Cuts at: 849 849
Size: 3845
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1864 1864
Size: 3845
FspI TGC'GCA
Cuts at: 1626 1626
Size: 3845
HaeII r_GCGC'y
Cuts at: 1279 1527 2757 3528 1279
Size: 248 1230 771 1596
Fragments arranged by size:
1596 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 419 451 729 761 1687 1717 1719 1749 419
Size: 32 278 32 926 30 2 30 2515
Fragments arranged by size:
2515 926 278 32 32 30 30 2
HincII GTy'rAC
Cuts at: 132 884 132
Size: 752 3093
HindIII A'AGCT_T
Cuts at: 283 654 283
Size: 371 3474
KasI G'GCGC_C
Cuts at: 1275 1523 1275
Size: 248 3597
KpnI G_GTAC'C
Cuts at: 865 865
Size: 3845
MluI A'CGCG_T
Cuts at: 3278 3278
Size: 3845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 783 2604 2788 783
Size: 1821 184 1840
Fragments arranged by size:
1840 1821 184
MscI TGG'CCA
Cuts at: 112 1606 112
Size: 1494 2351
MslI CAynn'nnrTG
Cuts at: 1834 1961 1834
Size: 127 3718
MspA1I CmG'CkG
Cuts at: 764 956 1270 1630 2414 2659 764
Size: 192 314 360 784 245 1950
Fragments arranged by size:
1950 784 360 314 245 192
NaeI GCC'GGC
Cuts at: 2026 2026
Size: 3845
NarI GG'CG_CC
Cuts at: 1276 1524 1276
Size: 248 3597
NcoI C'CATG_G
Cuts at: 107 107
Size: 3845
NgoMIV G'CCGG_C
Cuts at: 2024 2024
Size: 3845
NspI r_CATG'y
Cuts at: 898 1398 3001 898
Size: 500 1603 1742
Fragments arranged by size:
1742 1603 500
PciI A'CATG_T
Cuts at: 2997 2997
Size: 3845
PmeI GTTT'AAAC
Cuts at: 844 844
Size: 3845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2291 2323 2291
Size: 32 3813
PpuMI rG'GwC_Cy
Cuts at: 702 702
Size: 3845
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 272 304 272
Size: 32 3813
PstI C_TGCA'G
Cuts at: 186 892 1577 186
Size: 706 685 2454
Fragments arranged by size:
2454 706 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3845
PvuII CAG'CTG
Cuts at: 764 1270 1630 764
Size: 506 360 2979
Fragments arranged by size:
2979 506 360
RsrII CG'GwC_CG
Cuts at: 2040 2040
Size: 3845
SacI G_AGCT'C
Cuts at: 859 859
Size: 3845
SalI G'TCGA_C
Cuts at: 882 882
Size: 3845
SapI GCTCTTCn'nnn_
Cuts at: 1868 2078 1868
Size: 210 3635
SbfI CC_TGCA'GG
Cuts at: 892 892
Size: 3845
ScaI AGT'ACT
Cuts at: 3362 3362
Size: 3845
SfcI C'TryA_G
Cuts at: 33 161 182 539 888 1573 2541 2732 33
Size: 128 21 357 349 685 968 191 1146
Fragments arranged by size:
1146 968 685 357 349 191 128 21
SfoI GGC'GCC
Cuts at: 1277 1525 1277
Size: 248 3597
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3845
SmaI CCC'GGG
Cuts at: 867 867
Size: 3845
SmlI C'TyrA_G
Cuts at: 1000 1915 2629 2891 1000
Size: 915 714 262 1954
Fragments arranged by size:
1954 915 714 262
SnaBI TAC'GTA
Cuts at: 157 157
Size: 3845
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3845
SphI G_CATG'C
Cuts at: 898 1398 898
Size: 500 3345
SspI AAT'ATT
Cuts at: 833 833
Size: 3845
StyI C'CwwG_G
Cuts at: 107 979 1333 107
Size: 872 354 2619
Fragments arranged by size:
2619 872 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 744 1428 1561 2020 2229 744
Size: 684 133 459 209 2360
Fragments arranged by size:
2360 684 459 209 133
TatI w'GTAC_w
Cuts at: 758 3360 758
Size: 2602 1243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3362 3394 3362
Size: 32 3813
Tth111I GACn'n_nGTC
Cuts at: 1642 1642
Size: 3845
XbaI T'CTAG_A
Cuts at: 876 876
Size: 3845
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 816 1340 816
Size: 524 3321
XmaI C'CCGG_G
Cuts at: 865 865
Size: 3845
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI
BfrBI BglI BmgBI BmtI Bpu10I BsgI BsiWI BsmBI
BspEI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI SacII SanDI SexAI SfiI
SgrAI SrfI StuI SwaI XhoI XmnI ZraI