Restriction map for
pF1KB8594
(Circular) (Six-base) MAPSORT of: pF1KB8594.seq Check: 788 from: 1 to: 4553
pF1KB8594 4553 bp
With 182 enzymes: SgfI *
July 2, 2008 13:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 586 586
Size: 4553
AatII G_ACGT'C
Cuts at: 306 306
Size: 4553
AccI GT'mk_AC
Cuts at: 261 1591 261
Size: 1330 3223
Acc65I G'GTAC_C
Cuts at: 1569 1569
Size: 4553
AclI AA'CG_TT
Cuts at: 4393 4393
Size: 4553
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 2378 2810 3163 3825 260
Size: 2118 432 353 662 988
Fragments arranged by size:
2118 988 662 432 353
AflIII A'CryG_T
Cuts at: 1336 1905 3705 3986 1336
Size: 569 1800 281 1903
Fragments arranged by size:
1903 1800 569 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 140 140
Size: 4553
AlwNI CAG_nnn'CTG
Cuts at: 1858 3296 1858
Size: 1438 3115
ApaLI G'TGCA_C
Cuts at: 3391 3391
Size: 4553
ApoI r'AATT_y
Cuts at: 201 918 945 1027 1181 1369 1557 3906 4162
Size: 717 27 82 154 188 188 2349 256
Cuts at: 4162 201
Size: 592
Fragments arranged by size:
2349 717 592 256 188 188 154 82
27
AseI AT'TA_AT
Cuts at: 20 20
Size: 4553
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
AvaI C'yCGr_G
Cuts at: 1573 1573
Size: 4553
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 387 420 387
Size: 33 4520
BamHI G'GATC_C
Cuts at: 1578 1578
Size: 4553
BanI G'GyrC_C
Cuts at: 1569 1983 2231 2266 1569
Size: 414 248 35 3856
Fragments arranged by size:
3856 414 248 35
BanII G_rGCy'C
Cuts at: 636 1567 2597 636
Size: 931 1030 2592
Fragments arranged by size:
2592 1030 931
BbeI G_GCGC'C
Cuts at: 1987 2235 1987
Size: 248 4305
BbsI GAAGACnn'nnnn_
Cuts at: 506 1226 1322 506
Size: 720 96 3737
Fragments arranged by size:
3737 720 96
BbvCI CC'TCA_GC
Cuts at: 530 530
Size: 4553
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1210 1244 1210
Size: 34 4519
BciVI GTATCCnnnnn_n'
Cuts at: 388 2451 3507 4553 388
Size: 2063 1056 1046 388
Fragments arranged by size:
2063 1056 1046 388
BclI T'GATC_A
Cuts at: 2073 2073
Size: 4553
BglI GCCn_nnn'nGGC
Cuts at: 1088 1088
Size: 4553
BglII A'GATC_T
Cuts at: 2068 4105 2068
Size: 2037 2516
BlpI GC'TnA_GC
Cuts at: 1665 1665
Size: 4553
Bme1580I G_kGCm'C
Cuts at: 2178 2271 3395 2178
Size: 93 1124 3336
Fragments arranged by size:
3336 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 688 1765 1846 2181 4325 688
Size: 1077 81 335 2144 916
Fragments arranged by size:
2144 1077 916 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2920 2920
Size: 4553
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 113 113
Size: 4553
Bpu10I CC'TnA_GC
Cuts at: 530 530
Size: 4553
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1172 1294 1729 2608 3322 3620 1172
Size: 122 435 879 714 298 2105
Fragments arranged by size:
2105 879 714 435 298 122
BsaI GGTCTCn'nnnn_
Cuts at: 4257 4257
Size: 4553
BsaAI yAC'GTr
Cuts at: 1906 1906
Size: 4553
BsaBI GATnn'nnATC
Cuts at: 6 2067 4104 6
Size: 2061 2037 455
Fragments arranged by size:
2061 2037 455
BsaHI Gr'CG_yC
Cuts at: 303 1984 2232 2934 4430 303
Size: 1681 248 702 1496 426
Fragments arranged by size:
1681 1496 702 426 248
BsaWI w'CCGG_w
Cuts at: 1742 2263 2941 3352 3499 3773 1742
Size: 521 678 411 147 274 2522
Fragments arranged by size:
2522 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1326 1356 1326
Size: 30 4523
BseYI C'CCAG_C
Cuts at: 1217 1978 2736 3401 3809 1217
Size: 761 758 665 408 1961
Fragments arranged by size:
1961 761 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2095 2141 3371 104
Size: 1991 46 1230 1286
Fragments arranged by size:
1991 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 636 1344 1567 2345 2535 3395 636
Size: 708 223 778 190 860 1794
Fragments arranged by size:
1794 860 778 708 223 190
BsmI GAATG_Cn'
Cuts at: 213 1010 213
Size: 797 3756
Bsp1286I G_dGCh'C
Cuts at: 636 1344 1567 2178 2271 2345 2535 2597 3395
Size: 708 223 611 93 74 190 62 798
Cuts at: 3395 636
Size: 1794
Fragments arranged by size:
1794 798 708 611 223 190 93 74
62
BspHI T'CATG_A
Cuts at: 846 2985 846
Size: 2139 2414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 586 780 1603 1817 2119 2500 586
Size: 194 823 214 302 381 2639
Fragments arranged by size:
2639 823 381 302 214 194
BsrBI CCG'CTC
Cuts at: 50 2845 2899 4383 4548 50
Size: 2795 54 1484 165 55
Fragments arranged by size:
2795 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1206 2465 1206
Size: 1259 3294
BsrFI r'CCGG_y
Cuts at: 2551 2732 2551
Size: 181 4372
BssSI C'ACGA_G
Cuts at: 2824 3532 2824
Size: 708 3845
BstAPI GCAn_nnn'nTGC
Cuts at: 1930 1930
Size: 4553
BstBI TT'CG_AA
Cuts at: 2914 2914
Size: 4553
BstYI r'GATC_y
Cuts at: 1 1578 2045 2068 2403 2649 2955 2967 3053
Size: 1577 467 23 335 246 306 12 86
Cuts at: 3053 4105 1
Size: 1052 449
Fragments arranged by size:
1577 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 399 1462 107
Size: 292 1063 3198
Fragments arranged by size:
3198 1063 292
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2503 2684 2847 2503
Size: 181 163 4209
Fragments arranged by size:
4209 181 163
BtsI GCAGTG_nn'
Cuts at: 1527 1796 1527
Size: 269 4284
DraI TTT'AAA
Cuts at: 548 885 1552 548
Size: 337 667 3549
Fragments arranged by size:
3549 667 337
DrdI GACnn_nn'nnGTC
Cuts at: 2259 3603 2259
Size: 1344 3209
EaeI y'GGCC_r
Cuts at: 2138 2312 2703 2730 4075 2138
Size: 174 391 27 1345 2616
Fragments arranged by size:
2616 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2138 2138
Size: 4553
EarI CTCTTCn'nnn_
Cuts at: 279 957 1015 1055 2576 2786 279
Size: 678 58 40 1521 210 2046
Fragments arranged by size:
2046 1521 678 210 58 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2761 3503 3649 4363 2761
Size: 742 146 714 2951
Fragments arranged by size:
2951 742 714 146
EcoICRI GAG'CTC
Cuts at: 634 1565 634
Size: 931 3622
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 113 260 2378 2810 3163 3825 113
Size: 147 2118 432 353 662 841
Fragments arranged by size:
2118 841 662 432 353 147
EcoNI CCTnn'n_nnAGG
Cuts at: 311 311
Size: 4553
EcoO109I rG'GnC_Cy
Cuts at: 171 1692 171
Size: 1521 3032
EcoRI G'AATT_C
Cuts at: 1369 1557 1369
Size: 188 4365
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2572 2572
Size: 4553
FspI TGC'GCA
Cuts at: 1009 2334 1009
Size: 1325 3228
FspAI rTGC'GCAy
Cuts at: 1009 1009
Size: 4553
HaeII r_GCGC'y
Cuts at: 1987 2235 3465 4236 1987
Size: 248 1230 771 2304
Fragments arranged by size:
2304 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 225 257 526 558 1093 1125 2395 2425 2427
Size: 32 269 32 535 32 1270 30 2
Cuts at: 2427 2457 225
Size: 30 2321
Fragments arranged by size:
2321 1270 535 269 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 262 963 1592 262
Size: 701 629 3223
Fragments arranged by size:
3223 701 629
KasI G'GCGC_C
Cuts at: 1983 2231 1983
Size: 248 4305
KpnI G_GTAC'C
Cuts at: 1573 1573
Size: 4553
MluI A'CGCG_T
Cuts at: 3986 3986
Size: 4553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 810 3312 3496 810
Size: 2502 184 1867
Fragments arranged by size:
2502 1867 184
MscI TGG'CCA
Cuts at: 2314 2314
Size: 4553
MslI CAynn'nnrTG
Cuts at: 389 1073 2542 2669 389
Size: 684 1469 127 2273
Fragments arranged by size:
2273 1469 684 127
MspA1I CmG'CkG
Cuts at: 1664 1978 2338 3122 3367 1664
Size: 314 360 784 245 2850
Fragments arranged by size:
2850 784 360 314 245
NaeI GCC'GGC
Cuts at: 2734 2734
Size: 4553
NarI GG'CG_CC
Cuts at: 1984 2232 1984
Size: 248 4305
NcoI C'CATG_G
Cuts at: 107 399 107
Size: 292 4261
NgoMIV G'CCGG_C
Cuts at: 2732 2732
Size: 4553
NspI r_CATG'y
Cuts at: 1340 1606 2106 3709 1340
Size: 266 500 1603 2184
Fragments arranged by size:
2184 1603 500 266
PciI A'CATG_T
Cuts at: 1336 3705 1336
Size: 2369 2184
PmeI GTTT'AAAC
Cuts at: 1552 1552
Size: 4553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2999 3031 2999
Size: 32 4521
PpuMI rG'GwC_Cy
Cuts at: 171 171
Size: 4553
PsiI TTA'TAA
Cuts at: 752 752
Size: 4553
PstI C_TGCA'G
Cuts at: 597 1600 2285 597
Size: 1003 685 2865
Fragments arranged by size:
2865 1003 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4553
PvuII CAG'CTG
Cuts at: 1978 2338 1978
Size: 360 4193
RsrII CG'GwC_CG
Cuts at: 2748 2748
Size: 4553
SacI G_AGCT'C
Cuts at: 636 1567 636
Size: 931 3622
SalI G'TCGA_C
Cuts at: 260 1590 260
Size: 1330 3223
SapI GCTCTTCn'nnn_
Cuts at: 2576 2786 2576
Size: 210 4343
SbfI CC_TGCA'GG
Cuts at: 1600 1600
Size: 4553
ScaI AGT'ACT
Cuts at: 1137 4070 1137
Size: 2933 1620
SfcI C'TryA_G
Cuts at: 33 593 1596 2281 3249 3440 33
Size: 560 1003 685 968 191 1146
Fragments arranged by size:
1146 1003 968 685 560 191
SfoI GGC'GCC
Cuts at: 1985 2233 1985
Size: 248 4305
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
SmaI CCC'GGG
Cuts at: 1575 1575
Size: 4553
SmlI C'TyrA_G
Cuts at: 1187 1273 1708 2623 3337 3599 1187
Size: 86 435 915 714 262 2141
Fragments arranged by size:
2141 915 714 435 262 86
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4553
SphI G_CATG'C
Cuts at: 1606 2106 1606
Size: 500 4053
StyI C'CwwG_G
Cuts at: 107 399 450 637 1687 2041 107
Size: 292 51 187 1050 354 2619
Fragments arranged by size:
2619 1050 354 292 187 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1220 2136 2269 2728 2937 1220
Size: 916 133 459 209 2836
Fragments arranged by size:
2836 916 459 209 133
TatI w'GTAC_w
Cuts at: 1135 4068 1135
Size: 2933 1620
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4070 4102 4070
Size: 32 4521
Tth111I GACn'n_nGTC
Cuts at: 2350 2350
Size: 4553
XbaI T'CTAG_A
Cuts at: 628 1584 628
Size: 956 3597
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2048 2048
Size: 4553
XmaI C'CCGG_G
Cuts at: 1573 1573
Size: 4553
ZraI GAC'GTC
Cuts at: 304 304
Size: 4553
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AleI AloI ApaI AscI
AvrII BfrBI BmgBI BmtI BseRI BsgI BsiWI BsmBI
BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI