Restriction map for
pF1KB8592
(Circular) (Six-base) MAPSORT of: pF1KB8592.seq Check: 6234 from: 1 to: 5480
pF1KB8592 5480 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2409 2409
Size: 5480
AccI GT'mk_AC
Cuts at: 2518 2518
Size: 5480
Acc65I G'GTAC_C
Cuts at: 2496 2496
Size: 5480
AclI AA'CG_TT
Cuts at: 5320 5320
Size: 5480
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 1417 1562 1871 3305 3737 4090 4752 749
Size: 668 145 309 1434 432 353 662 1477
Fragments arranged by size:
1477 1434 668 662 432 353 309 145
AflIII A'CryG_T
Cuts at: 299 524 2352 2832 4632 4913 299
Size: 225 1828 480 1800 281 866
Fragments arranged by size:
1828 1800 866 480 281 225
AlwNI CAG_nnn'CTG
Cuts at: 2785 4223 2785
Size: 1438 4042
ApaLI G'TGCA_C
Cuts at: 4318 4318
Size: 5480
ApoI r'AATT_y
Cuts at: 400 437 516 1023 1462 1923 2341 2466 2484
Size: 37 79 507 439 461 418 125 18
Cuts at: 2484 4833 5089 400
Size: 2349 256 791
Fragments arranged by size:
2349 791 507 461 439 418 256 125
79 37 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 5480
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5480
AvaI C'yCGr_G
Cuts at: 2500 2500
Size: 5480
BamHI G'GATC_C
Cuts at: 2505 2505
Size: 5480
BanI G'GyrC_C
Cuts at: 2496 2910 3158 3193 2496
Size: 414 248 35 4783
Fragments arranged by size:
4783 414 248 35
BanII G_rGCy'C
Cuts at: 2494 3524 2494
Size: 1030 4450
BbeI G_GCGC'C
Cuts at: 2914 3162 2914
Size: 248 5232
BbsI GAAGACnn'nnnn_
Cuts at: 934 1423 1527 2123 934
Size: 489 104 596 4291
Fragments arranged by size:
4291 596 489 104
BciVI GTATCCnnnnn_n'
Cuts at: 3378 4434 5480 3378
Size: 1056 1046 3378
Fragments arranged by size:
3378 1056 1046
BclI T'GATC_A
Cuts at: 2331 2451 3000 2331
Size: 120 549 4811
Fragments arranged by size:
4811 549 120
BfrBI ATG'CAT
Cuts at: 2026 2026
Size: 5480
BglII A'GATC_T
Cuts at: 1416 2995 5032 1416
Size: 1579 2037 1864
Fragments arranged by size:
2037 1864 1579
BlpI GC'TnA_GC
Cuts at: 2592 2592
Size: 5480
Bme1580I G_kGCm'C
Cuts at: 2380 3105 3198 4322 2380
Size: 725 93 1124 3538
Fragments arranged by size:
3538 1124 725 93
BmrI ACTGGGnnnn_n'
Cuts at: 2692 2773 3108 5252 2692
Size: 81 335 2144 2920
Fragments arranged by size:
2920 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 302 3847 302
Size: 3545 1935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 464
Size: 5480
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 829 868 1122 2656 3535 4249 4547 577
Size: 252 39 254 1534 879 714 298 1510
Fragments arranged by size:
1534 1510 879 714 298 254 252 39
BsaI GGTCTCn'nnnn_
Cuts at: 5184 5184
Size: 5480
BsaAI yAC'GTr
Cuts at: 2242 2833 2242
Size: 591 4889
BsaBI GATnn'nnATC
Cuts at: 6 2994 5031 6
Size: 2988 2037 455
Fragments arranged by size:
2988 2037 455
BsaHI Gr'CG_yC
Cuts at: 2911 3159 3861 5357 2911
Size: 248 702 1496 3034
Fragments arranged by size:
3034 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2669 3190 3868 4279 4426 4700 2669
Size: 521 678 411 147 274 3449
Fragments arranged by size:
3449 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 207 237 207
Size: 30 5450
BseYI C'CCAG_C
Cuts at: 1593 2905 3663 4328 4736 1593
Size: 1312 758 665 408 2337
Fragments arranged by size:
2337 1312 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1271 2406 2458 1271
Size: 1135 52 4293
Fragments arranged by size:
4293 1135 52
BsiEI CG_ry'CG
Cuts at: 104 3022 3068 4298 104
Size: 2918 46 1230 1286
Fragments arranged by size:
2918 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2494 3272 3462 4322 2494
Size: 778 190 860 3652
Fragments arranged by size:
3652 860 778 190
BsiWI C'GTAC_G
Cuts at: 1254 1254
Size: 5480
BsmI GAATG_Cn'
Cuts at: 364 1477 364
Size: 1113 4367
Bsp1286I G_dGCh'C
Cuts at: 2380 2494 3105 3198 3272 3462 3524 4322 2380
Size: 114 611 93 74 190 62 798 3538
Fragments arranged by size:
3538 798 611 190 114 93 74 62
BspHI T'CATG_A
Cuts at: 3912 3912
Size: 5480
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1286 2409 2530 2744 3046 3427 1286
Size: 1123 121 214 302 381 3339
Fragments arranged by size:
3339 1123 381 302 214 121
BsrBI CCG'CTC
Cuts at: 50 3772 3826 5310 5475 50
Size: 3722 54 1484 165 55
Fragments arranged by size:
3722 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 130 3392 130
Size: 3262 2218
BsrFI r'CCGG_y
Cuts at: 3478 3659 3478
Size: 181 5299
BssSI C'ACGA_G
Cuts at: 287 3751 4459 287
Size: 3464 708 1308
Fragments arranged by size:
3464 1308 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2056 2857 2056
Size: 801 4679
BstBI TT'CG_AA
Cuts at: 3841 3841
Size: 5480
BstEII G'GTnAC_C
Cuts at: 534 534
Size: 5480
BstXI CCAn_nnnn'nTGG
Cuts at: 251 251
Size: 5480
BstYI r'GATC_y
Cuts at: 1 1416 2505 2972 2995 3330 3576 3882 3894
Size: 1415 1089 467 23 335 246 306 12
Cuts at: 3894 3980 5032 1
Size: 86 1052 449
Fragments arranged by size:
1415 1089 1052 467 449 335 306 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3430 3611 3774 3430
Size: 181 163 5136
Fragments arranged by size:
5136 181 163
BtsI GCAGTG_nn'
Cuts at: 1337 2723 1337
Size: 1386 4094
DraI TTT'AAA
Cuts at: 872 1019 1163 2479 872
Size: 147 144 1316 3873
Fragments arranged by size:
3873 1316 147 144
DrdI GACnn_nn'nnGTC
Cuts at: 2125 3186 4530 2125
Size: 1061 1344 3075
Fragments arranged by size:
3075 1344 1061
EaeI y'GGCC_r
Cuts at: 173 3065 3239 3630 3657 5002 173
Size: 2892 174 391 27 1345 651
Fragments arranged by size:
2892 1345 651 391 174 27
EagI C'GGCC_G
Cuts at: 3065 3065
Size: 5480
EarI CTCTTCn'nnn_
Cuts at: 482 755 766 1061 1398 3503 3713 482
Size: 273 11 295 337 2105 210 2249
Fragments arranged by size:
2249 2105 337 295 273 210 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3688 4430 4576 5290 3688
Size: 742 146 714 3878
Fragments arranged by size:
3878 742 714 146
EcoICRI GAG'CTC
Cuts at: 2492 2492
Size: 5480
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 749 1417 1562 1871 3305 3737 4090 4752
Size: 285 668 145 309 1434 432 353 662
Cuts at: 4752 464
Size: 1192
Fragments arranged by size:
1434 1192 668 662 432 353 309 285
145
EcoO109I rG'GnC_Cy
Cuts at: 2619 2619
Size: 5480
EcoRI G'AATT_C
Cuts at: 1923 2484 1923
Size: 561 4919
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 473 511 568 3499 473
Size: 38 57 2931 2454
Fragments arranged by size:
2931 2454 57 38
FspI TGC'GCA
Cuts at: 3261 3261
Size: 5480
HaeII r_GCGC'y
Cuts at: 2914 3162 4392 5163 2914
Size: 248 1230 771 3231
Fragments arranged by size:
3231 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 332 364 775 807 808 840 3322 3352 3354
Size: 32 411 32 1 32 2482 30 2
Cuts at: 3354 3384 332
Size: 30 2428
Fragments arranged by size:
2482 2428 411 32 32 32 30 30
2 1
HincII GTy'rAC
Cuts at: 1251 2233 2519 1251
Size: 982 286 4212
Fragments arranged by size:
4212 982 286
HindIII A'AGCT_T
Cuts at: 741 994 741
Size: 253 5227
KasI G'GCGC_C
Cuts at: 2910 3158 2910
Size: 248 5232
KpnI G_GTAC'C
Cuts at: 2500 2500
Size: 5480
MfeI C'AATT_G
Cuts at: 351 842 351
Size: 491 4989
MluI A'CGCG_T
Cuts at: 4913 4913
Size: 5480
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 167 252 4239 4423 167
Size: 85 3987 184 1224
Fragments arranged by size:
3987 1224 184 85
MscI TGG'CCA
Cuts at: 175 3241 175
Size: 3066 2414
MslI CAynn'nnrTG
Cuts at: 431 529 2240 3469 3596 431
Size: 98 1711 1229 127 2315
Fragments arranged by size:
2315 1711 1229 127 98
MspA1I CmG'CkG
Cuts at: 2591 2905 3265 4049 4294 2591
Size: 314 360 784 245 3777
Fragments arranged by size:
3777 784 360 314 245
NaeI GCC'GGC
Cuts at: 3661 3661
Size: 5480
NarI GG'CG_CC
Cuts at: 2911 3159 2911
Size: 248 5232
NgoMIV G'CCGG_C
Cuts at: 3659 3659
Size: 5480
NsiI A_TGCA'T
Cuts at: 2028 2028
Size: 5480
NspI r_CATG'y
Cuts at: 528 1955 2356 2437 2533 3033 4636 528
Size: 1427 401 81 96 500 1603 1372
Fragments arranged by size:
1603 1427 1372 500 401 96 81
PciI A'CATG_T
Cuts at: 524 2352 4632 524
Size: 1828 2280 1372
Fragments arranged by size:
2280 1828 1372
PmeI GTTT'AAAC
Cuts at: 2479 2479
Size: 5480
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3926 3958 3926
Size: 32 5448
PsiI TTA'TAA
Cuts at: 956 1454 1868 956
Size: 498 414 4568
Fragments arranged by size:
4568 498 414
PstI C_TGCA'G
Cuts at: 1085 1446 1641 2134 2527 3212 1085
Size: 361 195 493 393 685 3353
Fragments arranged by size:
3353 685 493 393 361 195
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5480
PvuII CAG'CTG
Cuts at: 2905 3265 2905
Size: 360 5120
RsrII CG'GwC_CG
Cuts at: 3675 3675
Size: 5480
SacI G_AGCT'C
Cuts at: 2494 2494
Size: 5480
SalI G'TCGA_C
Cuts at: 2517 2517
Size: 5480
SapI GCTCTTCn'nnn_
Cuts at: 482 3503 3713 482
Size: 3021 210 2249
Fragments arranged by size:
3021 2249 210
SbfI CC_TGCA'GG
Cuts at: 2527 2527
Size: 5480
ScaI AGT'ACT
Cuts at: 282 4997 282
Size: 4715 765
SfcI C'TryA_G
Cuts at: 33 1081 1442 1613 1637 2130 2523 3208 4176
Size: 1048 361 171 24 493 393 685 968
Cuts at: 4176 4367 33
Size: 191 1146
Fragments arranged by size:
1146 1048 968 685 493 393 361 191
171 24
SfoI GGC'GCC
Cuts at: 2912 3160 2912
Size: 248 5232
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5480
SmaI CCC'GGG
Cuts at: 2502 2502
Size: 5480
SmlI C'TyrA_G
Cuts at: 556 808 883 1137 2635 3550 4264 4526 556
Size: 252 75 254 1498 915 714 262 1510
Fragments arranged by size:
1510 1498 915 714 262 254 252 75
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5480
SphI G_CATG'C
Cuts at: 2533 3033 2533
Size: 500 4980
StuI AGG'CCT
Cuts at: 551 551
Size: 5480
StyI C'CwwG_G
Cuts at: 369 2614 2968 369
Size: 2245 354 2881
Fragments arranged by size:
2881 2245 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3063 3196 3655 3864 3063
Size: 133 459 209 4679
Fragments arranged by size:
4679 459 209 133
TatI w'GTAC_w
Cuts at: 280 357 451 1854 4995 280
Size: 77 94 1403 3141 765
Fragments arranged by size:
3141 1403 765 94 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 207 239 4997 5029 207
Size: 32 4758 32 658
Fragments arranged by size:
4758 658 32 32
Tth111I GACn'n_nGTC
Cuts at: 3277 3277
Size: 5480
XbaI T'CTAG_A
Cuts at: 1887 2511 1887
Size: 624 4856
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2975 2975
Size: 5480
XmaI C'CCGG_G
Cuts at: 2500 2500
Size: 5480
XmnI GAAnn'nnTTC
Cuts at: 775 1193 775
Size: 418 5062
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PciI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BseRI BsmBI BspEI BsrGI BssHII BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI NcoI NdeI NheI NotI NruI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI