Restriction map for
pF1KB8591
(Circular) (Six-base) MAPSORT of: pF1KB8591.seq Check: 5327 from: 1 to: 5423
pF1KB8591 5423 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 2461 2461
Size: 5423
Acc65I G'GTAC_C
Cuts at: 2439 2439
Size: 5423
AclI AA'CG_TT
Cuts at: 5263 5263
Size: 5423
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1164 2291 3248 3680 4033 4695 1164
Size: 1127 957 432 353 662 1892
Fragments arranged by size:
1892 1127 957 662 432 353
AflIII A'CryG_T
Cuts at: 2398 2775 4575 4856 2398
Size: 377 1800 281 2965
Fragments arranged by size:
2965 1800 377 281
AhdI GACnn_n'nnGTC
Cuts at: 1216 1767 1216
Size: 551 4872
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1058 1058
Size: 5423
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 225 257 225
Size: 32 5391
AlwNI CAG_nnn'CTG
Cuts at: 646 1006 2728 4166 646
Size: 360 1722 1438 1903
Fragments arranged by size:
1903 1722 1438 360
ApaI G_GGCC'C
Cuts at: 2076 2076
Size: 5423
ApaLI G'TGCA_C
Cuts at: 4261 4261
Size: 5423
ApoI r'AATT_y
Cuts at: 2427 4776 5032 2427
Size: 2349 256 2818
Fragments arranged by size:
2818 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5423
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5423
AvaI C'yCGr_G
Cuts at: 135 142 350 872 937 2443 135
Size: 7 208 522 65 1506 3115
Fragments arranged by size:
3115 1506 522 208 65 7
BamHI G'GATC_C
Cuts at: 318 2448 318
Size: 2130 3293
BanI G'GyrC_C
Cuts at: 225 346 529 1901 2439 2853 3101 3136 225
Size: 121 183 1372 538 414 248 35 2512
Fragments arranged by size:
2512 1372 538 414 248 183 121 35
BanII G_rGCy'C
Cuts at: 811 1518 2076 2220 2388 2437 3467 811
Size: 707 558 144 168 49 1030 2767
Fragments arranged by size:
2767 1030 707 558 168 144 49
BbeI G_GCGC'C
Cuts at: 350 2857 3105 350
Size: 2507 248 2668
Fragments arranged by size:
2668 2507 248
BbsI GAAGACnn'nnnn_
Cuts at: 955 955
Size: 5423
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5389
BciVI GTATCCnnnnn_n'
Cuts at: 213 3321 4377 5423 213
Size: 3108 1056 1046 213
Fragments arranged by size:
3108 1056 1046 213
BclI T'GATC_A
Cuts at: 2943 2943
Size: 5423
BglI GCCn_nnn'nGGC
Cuts at: 112 768 112
Size: 656 4767
BglII A'GATC_T
Cuts at: 1536 2938 4975 1536
Size: 1402 2037 1984
Fragments arranged by size:
2037 1984 1402
BlpI GC'TnA_GC
Cuts at: 190 632 1517 1522 1878 2535 190
Size: 442 885 5 356 657 3078
Fragments arranged by size:
3078 885 657 442 356 5
Bme1580I G_kGCm'C
Cuts at: 230 1238 2076 3048 3141 4265 230
Size: 1008 838 972 93 1124 1388
Fragments arranged by size:
1388 1124 1008 972 838 93
BmrI ACTGGGnnnn_n'
Cuts at: 1098 1684 1904 2390 2635 2716 3051 5195 1098
Size: 586 220 486 245 81 335 2144 1326
Fragments arranged by size:
2144 1326 586 486 335 245 220 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 276 1537 3790 276
Size: 1261 2253 1909
Fragments arranged by size:
2253 1909 1261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 940 1008 1271 1639 1722 1826 1969 940
Size: 68 263 368 83 104 143 4394
Fragments arranged by size:
4394 368 263 143 104 83 68
Bpu10I CC'TnA_GC
Cuts at: 1007 1058 1007
Size: 51 5372
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 825 2599 3478 4192 4490 616
Size: 209 1774 879 714 298 1549
Fragments arranged by size:
1774 1549 879 714 298 209
BsaI GGTCTCn'nnnn_
Cuts at: 387 5127 387
Size: 4740 683
BsaAI yAC'GTr
Cuts at: 2776 2776
Size: 5423
BsaBI GATnn'nnATC
Cuts at: 6 2937 4974 6
Size: 2931 2037 455
Fragments arranged by size:
2931 2037 455
BsaHI Gr'CG_yC
Cuts at: 347 2154 2854 3102 3804 5300 347
Size: 1807 700 248 702 1496 470
Fragments arranged by size:
1807 1496 702 700 470 248
BsaWI w'CCGG_w
Cuts at: 2612 3133 3811 4222 4369 4643 2612
Size: 521 678 411 147 274 3392
Fragments arranged by size:
3392 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2242 2272 2370 2400 2242
Size: 30 98 30 5265
Fragments arranged by size:
5265 98 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 508 1773 2378 508
Size: 1265 605 3553
Fragments arranged by size:
3553 1265 605
BseYI C'CCAG_C
Cuts at: 655 780 810 1238 2361 2848 3606 4271 4679
Size: 125 30 428 1123 487 758 665 408
Cuts at: 4679 655
Size: 1399
Fragments arranged by size:
1399 1123 758 665 487 428 408 125
30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1907 1907
Size: 5423
BsiEI CG_ry'CG
Cuts at: 104 210 2965 3011 4241 104
Size: 106 2755 46 1230 1286
Fragments arranged by size:
2755 1286 1230 106 46
BsiHKAI G_wGCw'C
Cuts at: 1466 1518 1745 2437 3215 3405 4265 1466
Size: 52 227 692 778 190 860 2624
Fragments arranged by size:
2624 860 778 692 227 190 52
BsmBI CGTCTCn'nnnn_
Cuts at: 830 1657 830
Size: 827 4596
Bsp1286I G_dGCh'C
Cuts at: 230 811 1238 1466 1518 1745 2076 2220 2388
Size: 581 427 228 52 227 331 144 168
Cuts at: 2388 2437 3048 3141 3215 3405 3467 4265 230
Size: 49 611 93 74 190 62 798 1388
Fragments arranged by size:
1388 798 611 581 427 331 228 227
190 168 144 93 74 62 52 49
BspHI T'CATG_A
Cuts at: 3855 3855
Size: 5423
BspMI ACCTGCnnnn'nnnn_
Cuts at: 573 998 2473 2687 2989 3370 573
Size: 425 1475 214 302 381 2626
Fragments arranged by size:
2626 1475 425 381 302 214
BsrBI CCG'CTC
Cuts at: 50 194 221 2280 3715 3769 5253 5418 50
Size: 144 27 2059 1435 54 1484 165 55
Fragments arranged by size:
2059 1484 1435 165 144 55 54 27
BsrDI GCAATG_nn'
Cuts at: 497 3335 497
Size: 2838 2585
BsrFI r'CCGG_y
Cuts at: 111 3421 3602 111
Size: 3310 181 1932
Fragments arranged by size:
3310 1932 181
BssSI C'ACGA_G
Cuts at: 3694 4402 3694
Size: 708 4715
BstAPI GCAn_nnn'nTGC
Cuts at: 2800 2800
Size: 5423
BstBI TT'CG_AA
Cuts at: 3784 3784
Size: 5423
BstEII G'GTnAC_C
Cuts at: 1431 1959 1431
Size: 528 4895
BstXI CCAn_nnnn'nTGG
Cuts at: 723 818 1912 723
Size: 95 1094 4234
Fragments arranged by size:
4234 1094 95
BstYI r'GATC_y
Cuts at: 1 318 1536 2448 2915 2938 3273 3519 3825
Size: 317 1218 912 467 23 335 246 306
Cuts at: 3825 3837 3923 4975 1
Size: 12 86 1052 449
Fragments arranged by size:
1218 1052 912 467 449 335 317 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 422 422
Size: 5423
BtgI C'CryG_G
Cuts at: 797 907 1835 1973 797
Size: 110 928 138 4247
Fragments arranged by size:
4247 928 138 110
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3373 3554 3717 3373
Size: 181 163 5079
Fragments arranged by size:
5079 181 163
BtsI GCAGTG_nn'
Cuts at: 728 1508 2316 2666 728
Size: 780 808 350 3485
Fragments arranged by size:
3485 808 780 350
DraI TTT'AAA
Cuts at: 2422 2422
Size: 5423
DraIII CAC_nnn'GTG
Cuts at: 1431 2082 1431
Size: 651 4772
DrdI GACnn_nn'nnGTC
Cuts at: 3129 4473 3129
Size: 1344 4079
EaeI y'GGCC_r
Cuts at: 207 603 1014 1374 1647 2297 3008 3182 3573
Size: 396 411 360 273 650 711 174 391
Cuts at: 3573 3600 4945 207
Size: 27 1345 685
Fragments arranged by size:
1345 711 685 650 411 396 391 360
273 174 27
EagI C'GGCC_G
Cuts at: 207 3008 207
Size: 2801 2622
EarI CTCTTCn'nnn_
Cuts at: 3446 3656 3446
Size: 210 5213
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3631 4373 4519 5233 3631
Size: 742 146 714 3821
Fragments arranged by size:
3821 742 714 146
EcoICRI GAG'CTC
Cuts at: 1516 2435 1516
Size: 919 4504
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 940 1008 1164 1271 1639 1722 1826 1969 2291
Size: 68 156 107 368 83 104 143 322
Cuts at: 2291 3248 3680 4033 4695 940
Size: 957 432 353 662 1668
Fragments arranged by size:
1668 957 662 432 368 353 322 156
143 107 104 83 68
EcoNI CCTnn'n_nnAGG
Cuts at: 1681 1681
Size: 5423
EcoO109I rG'GnC_Cy
Cuts at: 139 663 933 1003 1686 2562 139
Size: 524 270 70 683 876 3000
Fragments arranged by size:
3000 876 683 524 270 70
EcoRI G'AATT_C
Cuts at: 2427 2427
Size: 5423
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3442 3442
Size: 5423
FspI TGC'GCA
Cuts at: 1850 3204 1850
Size: 1354 4069
HaeII r_GCGC'y
Cuts at: 350 1982 2857 3105 4335 5106 350
Size: 1632 875 248 1230 771 667
Fragments arranged by size:
1632 1230 875 771 667 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 310 342 1202 1234 1244 1276 1500 1532 1739
Size: 32 860 32 10 32 224 32 207
Cuts at: 1739 1753 1768 1771 1785 1800 2242 2274 2395
Size: 14 15 3 14 15 442 32 121
Cuts at: 2395 2427 3265 3295 3297 3327 310
Size: 32 838 30 2 30 2406
Fragments arranged by size:
2406 860 838 442 224 207 121 32
32 32 32 32 32 30 30 15
15 14 14 10 3 2
HincII GTy'rAC
Cuts at: 2462 2462
Size: 5423
KasI G'GCGC_C
Cuts at: 346 2853 3101 346
Size: 2507 248 2668
Fragments arranged by size:
2668 2507 248
KpnI G_GTAC'C
Cuts at: 2443 2443
Size: 5423
MluI A'CGCG_T
Cuts at: 4856 4856
Size: 5423
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1167 4182 4366 1167
Size: 3015 184 2224
Fragments arranged by size:
3015 2224 184
MscI TGG'CCA
Cuts at: 1376 2299 3184 1376
Size: 923 885 3615
Fragments arranged by size:
3615 923 885
MslI CAynn'nnrTG
Cuts at: 3412 3539 3412
Size: 127 5296
MspA1I CmG'CkG
Cuts at: 636 646 1369 1592 1846 2289 2534 2848 3208
Size: 10 723 223 254 443 245 314 360
Cuts at: 3208 3992 4237 636
Size: 784 245 1822
Fragments arranged by size:
1822 784 723 443 360 314 254 245
245 223 10
NaeI GCC'GGC
Cuts at: 113 3604 113
Size: 3491 1932
NarI GG'CG_CC
Cuts at: 347 2854 3102 347
Size: 2507 248 2668
Fragments arranged by size:
2668 2507 248
NcoI C'CATG_G
Cuts at: 1973 1973
Size: 5423
NgoMIV G'CCGG_C
Cuts at: 111 3602 111
Size: 3491 1932
NspI r_CATG'y
Cuts at: 1855 2402 2476 2976 4579 1855
Size: 547 74 500 1603 2699
Fragments arranged by size:
2699 1603 547 500 74
PasI CC'CwG_GG
Cuts at: 1966 1966
Size: 5423
PciI A'CATG_T
Cuts at: 2398 4575 2398
Size: 2177 3246
PflMI CCAn_nnn'nTGG
Cuts at: 1372 2082 2227 1372
Size: 710 145 4568
Fragments arranged by size:
4568 710 145
PfoI T'CCnGG_A
Cuts at: 1331 1331
Size: 5423
PmeI GTTT'AAAC
Cuts at: 2422 2422
Size: 5423
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 3869
Size: 32 5391
PpuMI rG'GwC_Cy
Cuts at: 139 663 933 1686 139
Size: 524 270 753 3876
Fragments arranged by size:
3876 753 524 270
PshAI GACnn'nnGTC
Cuts at: 263 470 263
Size: 207 5216
PspOMI G'GGCC_C
Cuts at: 2072 2072
Size: 5423
PstI C_TGCA'G
Cuts at: 1037 2470 3155 1037
Size: 1433 685 3305
Fragments arranged by size:
3305 1433 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5423
PvuII CAG'CTG
Cuts at: 646 1369 1592 1846 2848 3208 646
Size: 723 223 254 1002 360 2861
Fragments arranged by size:
2861 1002 723 360 254 223
RsrII CG'GwC_CG
Cuts at: 3618 3618
Size: 5423
SacI G_AGCT'C
Cuts at: 1518 2437 1518
Size: 919 4504
SalI G'TCGA_C
Cuts at: 2460 2460
Size: 5423
SanDI GG'GwC_CC
Cuts at: 139 139
Size: 5423
SapI GCTCTTCn'nnn_
Cuts at: 3446 3656 3446
Size: 210 5213
SbfI CC_TGCA'GG
Cuts at: 1037 2470 1037
Size: 1433 3990
ScaI AGT'ACT
Cuts at: 4940 4940
Size: 5423
SfcI C'TryA_G
Cuts at: 33 152 441 1033 1394 1841 2241 2466 3151
Size: 119 289 592 361 447 400 225 685
Cuts at: 3151 4119 4310 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 685 592 447 400 361 289
225 191 119
SfoI GGC'GCC
Cuts at: 348 2855 3103 348
Size: 2507 248 2668
Fragments arranged by size:
2668 2507 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5423
SmaI CCC'GGG
Cuts at: 137 144 352 2445 137
Size: 7 208 2093 3115
Fragments arranged by size:
3115 2093 208 7
SmlI C'TyrA_G
Cuts at: 595 804 2578 3493 4207 4469 595
Size: 209 1774 915 714 262 1549
Fragments arranged by size:
1774 1549 915 714 262 209
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5423
SphI G_CATG'C
Cuts at: 2476 2976 2476
Size: 500 4923
StyI C'CwwG_G
Cuts at: 1973 2557 2911 1973
Size: 584 354 4485
Fragments arranged by size:
4485 584 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1355 3006 3139 3598 3807 1355
Size: 1651 133 459 209 2971
Fragments arranged by size:
2971 1651 459 209 133
TatI w'GTAC_w
Cuts at: 4938 4938
Size: 5423
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1937 1969 2321 2353 4940 4972 1937
Size: 32 352 32 2587 32 2388
Fragments arranged by size:
2587 2388 352 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1690 3220 1690
Size: 1530 3893
XbaI T'CTAG_A
Cuts at: 2454 2454
Size: 5423
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1246 1697 1880 2918 1246
Size: 451 183 1038 3751
Fragments arranged by size:
3751 1038 451 183
XmaI C'CCGG_G
Cuts at: 135 142 350 2443 135
Size: 7 208 2093 3115
Fragments arranged by size:
3115 2093 208 7
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI BsiWI BsmI BspEI
BsrGI BssHII BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PsiI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI